Increased Adenine Nucleotide Degradation in Skeletal Muscle Atrophy

Adenine nucleotides (AdNs: ATP, ADP, AMP) are essential biological compounds that facilitate many necessary cellular processes by providing chemical energy, mediating intracellular signaling, and regulating protein metabolism and solubilization. A dramatic reduction in total AdNs is observed in atrophic skeletal muscle across numerous disease states and conditions, such as cancer, diabetes, chronic kidney disease, heart failure, COPD, sepsis, muscular dystrophy, denervation, disuse, and sarcopenia. The reduced AdNs in atrophic skeletal muscle are accompanied by increased expression/activities of AdN degrading enzymes and the accumulation of degradation products (IMP, hypoxanthine, xanthine, uric acid), suggesting that the lower AdN content is largely the result of increased nucleotide degradation. Furthermore, this characteristic decrease of AdNs suggests that increased nucleotide degradation contributes to the general pathophysiology of skeletal muscle atrophy. In view of the numerous energetic, and non-energetic, roles of AdNs in skeletal muscle, investigations into the physiological consequences of AdN degradation may provide valuable insight into the mechanisms of muscle atrophy

Evidence for deceleration in the radio jets of GRS1915+105?

There is currently a clear discrepancy in the proper motions measured on different angular scales in the approaching radio jets of the black hole X-ray binary GRS1915+105. Lower velocities were measured with the Very Large Array (VLA) prior to 1996 than were subsequently found from higher-resolution observations made with the Very Long Baseline Array and the Multi-Element Radio Linked Interferometer Network. We initiated an observing campaign to use all three arrays to attempt to track the motion of the jet knots from the 2006 February outburst of the source, giving us unprecedented simultaneous coverage of all angular scales, from milliarcsecond scales out to arcsecond scales. The derived proper motion, which was dominated by the VLA measurements, was found to be 17.0 mas per day, demonstrating that there has been no significant permanent change in the properties of the jets since 1994. We find no conclusive evidence for deceleration of the jet knots, unless this occurs within 70 mas of the core. We discuss possible causes for the varying proper motions recorded in the literature.Comment: Accepted for publication in MNRAS. 13 pages, 10 figure

Reassessment of vertebrate ichnotaxa from the Upper Carboniferous ‘Fern Ledges’, Lancaster Formation, Saint John, New Brunswick, Canada

Vertebrate ichnotaxa described by George Frederic Matthew in 1910 from the Upper Carboniferous (Lower Pennsylvanian) ‘Fern Ledges’ of Saint John, New Brunswick, were dismissed as dubious trackways by previous authors. Thus, three new ichnospecies Matthew described appeared in the 1975 Treatise on Invertebrate Paleontology as “unrecognized or unrecognizable” and were mostly forgotten by vertebrate ichnologists. These traces include Hylopus (?) variabilis, Nanopus (?) vetustus and Bipezia bilobata. One ichnospecies, Hylopus (?) variabilis, here is retained as a valid tetrapod footprint ichnotaxon and reassigned to the ichnogenus Limnopus as a new combination, together with other poorly preserved specimens Matthew labeled, but never described. Nanopus (?) vetustus and Bipezia bilobata named by Matthew in the same paper, have been reexamined and remain as nomina dubia.

First e-VLBI observations of GRS 1915+105

We present results from the first successful open call e-VLBI science run, observing the X-ray binary GRS 1915+105. e-VLBI science allows the rapid production of VLBI radio maps, within hours of an observation rather than weeks, facilitating a decision for follow-up observations. A total of 6 telescopes observing at 5 GHz across the European VLBI Network (EVN) were correlated in real time at the Joint Institute for VLBI in Europe (JIVE). Constant data rates of 128 Mbps were transferred from each telescope, giving 4 TB of raw sampled data over the 12 hours of the whole experiment. Throughout this, GRS 1915+105 was observed for a total of 5.5 hours, producing 2.8 GB of visibilities of correlated data. A weak flare occurred during our observations, and we detected a slightly resolved component of 2.7 x 1.2 milliarcsecond with a position angle of 140 (+/-2) degrees. The peak brightness was 10.2 mJy per beam, with a total integrated radio flux of 11.1 mJy.Comment: Accepted for publication in MNRAS 4 pages, 3 figure

A transient relativistic radio jet from Cygnus X-1

We report the first observation of a transient relativistic jet from the canonical black hole candidate, Cygnus X-1, obtained with the Multi-Element Radio-Linked Interferometer Network (MERLIN). The jet was observed in only one of six epochs of MERLIN imaging of the source during a phase of repeated X-ray spectral transitions in 2004 Jan--Feb, and this epoch corresponded to the softest 1.5-12 keV X-ray spectrum. With only a single epoch revealing the jet, we cannot formally constrain its velocity. Nevertheless, several lines of reasoning suggest that the jet was probably launched 0.5-4.0 days before this brightening, corresponding to projected velocities of 0.2c < v_app < 1.6c, and an intrinsic velocity of > 0.3c. We also report the occurrence of a major radio flare from Cyg X-1, reaching a flux density of ~120 mJy at 15 GHz, and yet not associated with any resolvable radio emission, despite a concerted effort with MERLIN. We discuss the resolved jet in terms of the recently proposed 'unified model' for the disc-jet coupling in black hole X-ray binaries, and tentatively identify the 'jet line' for Cyg X-1. The source is consistent with the model in the sense that a steady jet appears to persist initially when the X-ray spectrum starts softening, and that once the spectral softening is complete the core radio emission is suppressed and transient ejecta / shock observed. However, there are some anomalies, and Cyg X-1 clearly does not behave like a normal black hole transient in progressing to the canonical soft / thermal state once the ejection event has happened.Comment: Accepted for publication in MNRA

Multiple relativistic outbursts of GRS 1915+105: radio emission and internal shocks

We present 5-GHz MERLIN radio images of the microquasar GRS 1915+105 during two separate outbursts in 2001 March and 2001 July, following the evolution of the jet components as they move outwards from the core of the system. Proper motions constrain the intrinsic jet speed to be >0.57c, but the uncertainty in the source distance prevents an accurate determination of the jet speed. No deceleration is observed in the jet components out to an angular separation of about 300mas. Linear polarisation is observed in the approaching jet component, with a gradual rotation in position angle and a decreasing fractional polarisation with time. Our data lend support to the internal shock model whereby the jet velocity increases leading to internal shocks in the pre-existing outflow before the jet switches off. The compact nuclear jet is seen to re-establish itself within two days, and is visible as core emission at all epochs. The energetics of the source are calculated for the possible range of distances; a minimum power of 1-10 per cent of the Eddington luminosity is required to launch the jet.Comment: 18 pages, 14 figures, accepted for publication in MNRAS. For higher-resolution versions of Figures 3, 5, and 12, see http://remote.science.uva.nl/~jmiller/grs1915/figures.htm

Controls on the composition and lability of dissolved organic matter in Siberia's Kolyma River basin

High-latitude northern rivers export globally significant quantities of dissolved organic carbon (DOC) to the Arctic Ocean. Climate change, and its associated impacts on hydrology and potential mobilization of ancient organic matter from permafrost, is likely to modify the flux, composition, and thus biogeochemical cycling and fate of exported DOC in the Arctic. This study examined DOC concentration and the composition of dissolved organic matter (DOM) across the hydrograph in Siberia's Kolyma River, with a particular focus on the spring freshet period when the majority of the annual DOC load is exported. The composition of DOM within the Kolyma basin was characterized using absorbance-derived measurements (absorbance coefficienta330, specific UV absorbance (SUVA254), and spectral slope ratio SR) and fluorescence spectroscopy (fluorescence index and excitation-emission matrices (EEMs)), including parallel factor analyses of EEMs. Increased surface runoff during the spring freshet led to DOM optical properties indicative of terrestrial soil inputs with high humic-like fluorescence, SUVA254, and low SRand fluorescence index (FI). Under-ice waters, in contrast, displayed opposing trends in optical properties representing less aromatic, lower molecular weight DOM. We demonstrate that substantial losses of DOC can occur via biological (∼30% over 28 days) and photochemical pathways (>29% over 14 days), particularly in samples collected during the spring freshet. The emerging view is therefore that of a more dynamic and labile carbon pool than previously thought, where DOM composition plays a fundamental role in controlling the fate and removal of DOC at a pan-Arctic scale

Luciferin Amides Enable in Vivo Bioluminescence Detection of Endogenous Fatty Acid Amide Hydrolase Activity

Firefly luciferase is homologous to fatty acyl-CoA synthetases. We hypothesized that the firefly luciferase substrate d-luciferin and its analogs are fatty acid mimics that are ideally suited to probe the chemistry of enzymes that release fatty acid products. Here, we synthesized luciferin amides and found that these molecules are hydrolyzed to substrates for firefly luciferase by the enzyme fatty acid amide hydrolase (FAAH). In the presence of luciferase, these molecules enable highly sensitive and selective bioluminescent detection of FAAH activity in vitro, in live cells, and in vivo. The potency and tissue distribution of FAAH inhibitors can be imaged in live mice, and luciferin amides serve as exemplary reagents for greatly improved bioluminescence imaging in FAAH-expressing tissues such as the brain

A weak compact jet in a soft state of Cygnus X-1

We present evidence for the presence of a weak compact jet during a soft X-ray state of Cygnus X-1. Very-high-resolution radio observations were taken with the VLBA, EVN and MERLIN during a hard-to-soft spectral state change, showing the hard state jet to be suppressed by a factor of about 3-5 in radio flux and unresolved to direct imaging observations (i.e. < 1 mas at 4 cm). High time-resolution X-ray observations with the RXTE-PCA were also taken during the radio monitoring period, showing the source to make the transition from the hard state to a softer state (via an intermediate state), although the source may never have reached the canonical soft state. Using astrometric VLBI analysis and removing proper motion, parallax and orbital motion signatures, the residual positions show a scatter of ~0.2 mas (at 4 cm) and ~3 mas (at 13 cm) along the position angle of the known jet axis; these residuals suggest there is a weak unresolved outflow, with varying size or opacity, during intermediate and soft X-ray states. Furthermore, no evidence was found for extended knots or shocks forming within the jet during the state transition, suggesting the change in outflow rate may not be sufficiently high to produce superluminal knots.Comment: Accepted in MNRAS; 4 figures and 1 tabl