Sharpening the predictions of big-bang nucleosynthesis

Motivated by the recent measurement of the primeval abundance of deuterium, we re-examine the nuclear inputs to big-bang nucleosynthesis (BBN). Using Monte-Carlo realization of the nuclear cross-section data to directly estimate the theoretical uncertainties for the yields of D, 3-He and 7-Li, we show that previous estimates were a factor of 2 too large. We sharpen the BBN determination of the baryon density based upon deuterium, rho_B = (3.6 +/- 0.4) * 10^{-31} g/cm^3 (Omega_B h^2 = 0.019 +/- 0.0024), which leads to a predicted 4-He abundance, Y_P = 0.246 +/- 0.0014 and a stringent limit to the equivalent number of light neutrino species: N_nu < 3.20 (all at 95% cl). The predicted 7-Li abundance, 7-Li/H = (3.5 + 1.1 - 0.9) * 10^{-10}, is higher than that observed in pop II stars, (1.7 +/- 0.3) * 10^{-10} (both, 95% cl). We identify key reactions and the energies where further work is needed.Comment: 5 pages, 4 figures (epsfig), REVTeX; submitted to Phys. Rev. Let

Elliptic and hyperelliptic magnetohydrodynamic equilibria

The present study is a continuation of a previous one on "hyperelliptic" axisymmetric equilibria started in [Tasso and Throumoulopoulos, Phys. Plasmas 5, 2378 (1998)]. Specifically, some equilibria with incompressible flow nonaligned with the magnetic field and restricted by appropriate side conditions like "isothermal" magnetic surfaces, "isodynamicity" or P + B^2/2 constant on magnetic surfaces are found to be reducible to elliptic integrals. The third class recovers recent equilibria found in [Schief, Phys. Plasmas 10, 2677 (2003)]. In contrast to field aligned flows, all solutions found here have nonzero toroidal magnetic field on and elliptic surfaces near the magnetic axis.Comment: 9 page

New Models of Tetrahymena Telomerase RNA from Experimentally Derived Constraints and Modeling

The telomerase ribonucleoprotein complex ensures complete replication of eukaryotic chromosomes. Telomerase RNA, TER, provides the template for replicating the G-rich strand of telomeric DNA, provides an anchor site for telomerase-associated proteins, and participates in catalysis through several incompletely characterized mechanisms. A major impediment towards understanding its non-templating roles is the absence of high content structural information for TER within the telomerase complex. Here, we used selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) to examine the structure of Tetrahymena TER free in solution and bound to tTERT in the minimal telomerase RNP. We discovered a striking difference in the two conformations and established direct evidence for base pair triples in the tTER pseudoknot. We then used SHAPE data, previously published FRET data, and biochemical inference to model the structure of tTER using discrete molecular dynamics simulations. The resulting tTER structure was docked with a homology model of tTERT to characterize the conformational changes of tTER that attend binding to tTERT. Free in solution, tTER appears to contain four pairing regions: stems I, II, and IV, which are present in the commonly accepted structure, and stem III, a large paired region that encompasses the template and pseudoknot domains. Our interpretation of the data and subsequent modeling affords a molecular model for telomerase assemblage in which a large stem III of tTER unwinds to allow proper association of the template with the tTERT active site and formation of the pseudoknot. Additionally, analysis of our SHAPE data and previous enzymatic footpinting allows us to propose a model for stem-loop IV function in which tTERT is activated by binding stem IV in the major grove of the helix-capping loop

A 2MASS All-Sky View of the Sagittarius Dwarf Galaxy: Variation of the Metallicity Distribution Function Along the Sagittarius Stream

We present reliable measurements of the metallicity distribution function (MDF) at different points along the tidal stream of the Sagittarius (Sgr) dwarf spheroidal (dSph) galaxy, based on high resolution, echelle spectroscopy of candidate M giant members of the Sgr system. The Sgr MDF is found to evolve significantly from a median [Fe/H] ~-0.4 in the core to ~-1.1 dex over a Sgr leading arm length representing ~2.5-3.0 Gyr of dynamical (i.e. tidal stripping) age. This is direct evidence that there can be significant chemical differences between current dSph satellites and the bulk of the stars they have contributed to the halo. Our results suggest that Sgr experienced a significant change in binding energy over the past several Gyr, which has substantially decreased its tidal boundary across a radial range over which there must have been a significant metallicity gradient in the progenitor galaxy. By accounting for MDF variation along the debris arms, we approximate the MDF Sgr would have had several Gyr ago. We also analyze the MDF of a moving group of M giants we previously discovered towards the North Galactic Cap having opposite radial velocities to the infalling Sgr leading arm stars there and propose that most of these represent Sgr trailing arm stars overlapping the Sgr leading arm in this part of the sky. If so, these trailing arm stars further demonstrate the strong MDF evolution within the Sgr stream.Comment: The new version is a much-expanded version moved from ApJL to ApJ and will appear in Dec. 1 editio

The pseudophosphatase MK-STYX interacts with G3BP and decreases stress granule formation

MK-STYX [MAPK (mitogen-activated protein kinase) phospho-serine/threonine/tyrosine-binding protein] is a pseudophosphatase member of the dual-specificity phosphatase subfamily of the PTPs (protein tyrosine phosphatases). MK-STYX is catalytically inactive due to the absence of two amino acids from the signature motif that are essential for phosphatase activity. The nucleophilic cysteine residue and the adjacent histidine residue, which are conserved in all active dual-specificity phosphatases, are replaced by serine and phenylalanine residues respectively in MK-STYX. Mutations to introduce histidine and cysteine residues into the active site of MK-STYX generated an active phosphatase. Using MS, we identified G3BP1 [Ras-GAP (GTPase-activating protein) SH3 (Src homology 3) domain-binding protein-1], a regulator of Ras signalling, as a binding partner of MK-STYX. We observed that G3BP1 bound to native MK-STYX; however, binding to the mutant catalytically active form of MK-STYX was dramatically reduced. G3BP1 is also an RNA-binding protein with endoribonuclease activity that is recruited to ‘stress granules’ after stress stimuli. Stress granules are large subcellular structures that serve as sites of mRNA sorting, in which untranslated mRNAs accumulate. We have shown that expression of MK-STYX inhibited stress granule formation induced either by aresenite or expression of G3BP itself; however, the catalytically active mutant MK-STYX was impaired in its ability to inhibit G3BP-induced stress granule assembly. These results reveal a novel facet of the function of a member of the PTP family, illustrating a role for MK-STYX in regulating the ability of G3BP1 to integrate changes in growth-factor stimulation and environmental stress with the regulation of protein synthesis

MiRNA Profile Associated with Replicative Senescence, Extended Cell Culture, and Ectopic Telomerase Expression in Human Foreskin Fibroblasts

Senescence is a highly regulated process that limits cellular replication by enforcing a G1 arrest in response to various stimuli. Replicative senescence occurs in response to telomeric DNA erosion, and telomerase expression can offset replicative senescence leading to immortalization of many human cells. Limited data exists regarding changes of microRNA (miRNA) expression during senescence in human cells and no reports correlate telomerase expression with regulation of senescence-related miRNAs. We used miRNA microarrays to provide a detailed account of miRNA profiles for early passage and senescent human foreskin (BJ) fibroblasts as well as early and late passage immortalized fibroblasts (BJ-hTERT) that stably express the human telomerase reverse transcriptase subunit hTERT. Selected miRNAs that were differentially expressed in senescence were assayed for expression in quiescent cells to identify miRNAs that are specifically associated with senescence-associated growth arrest. From this group of senescence-associated miRNAs, we confirmed the ability of miR-143 to induce growth arrest after ectopic expression in young fibroblasts. Remarkably, miR-143 failed to induce growth arrest in BJ-hTERT cells. Importantly, the comparison of late passage immortalized fibroblasts to senescent wild type fibroblasts reveals that miR-146a, a miRNA with a validated role in regulating the senescence associated secretory pathway, is also regulated during extended cell culture independently of senescence. The discovery that miRNA expression is impacted by expression of ectopic hTERT as well as extended passaging in immortalized fibroblasts contributes to a comprehensive understanding of the connections between telomerase expression, senescence and processes of cellular aging

Disgust sensitivity relates to attitudes toward gay men and lesbian women across 31 nations

Previous work has reported a relation between pathogen-avoidance motivations and prejudice toward various social groups, including gay men and lesbian women. It is currently unknown whether this association is present across cultures, or specific to North America. Analyses of survey data from adult heterosexuals ( N = 11,200) from 31 countries showed a small relation between pathogen disgust sensitivity (an individual-difference measure of pathogen-avoidance motivations) and measures of antigay attitudes. Analyses also showed that pathogen disgust sensitivity relates not only to antipathy toward gay men and lesbians, but also to negativity toward other groups, in particular those associated with violations of traditional sexual norms (e.g., prostitutes). These results suggest that the association between pathogen-avoidance motivations and antigay attitudes is relatively stable across cultures and is a manifestation of a more general relation between pathogen-avoidance motivations and prejudice towards groups associated with sexual norm violations

Big-bang Nucleosynthesis Enters the Precision Era

The last parameter of big-bang nucleosynthesis, the baryon density, is being pinned down by measurements of the deuterium abundance in high-redshift hydrogen clouds. When it is determined, it will fix the primeval light-element abundances. D, ^3He and ^7Li will become ``tracers'' for the study of Galactic and stellar chemical evolution, and big-bang nucleosynthesis will become an even sharper probe of particle physics, e.g., the bound to the number of light neutrino species will be tightened significantly. Two key tests of the consistency of the standard theory are on the horizon: an independent, high-precision determination of the baryon density from anisotropy of the cosmic background radiation and a precision determination of the primeval $^4$He abundance.Comment: 19 pages Latex; 8 eps figures; submitted to Rev Mod Phys (Colloquia

Consistent patterns of common species across tropical tree communities

Trees structure the Earth’s most biodiverse ecosystem, tropical forests. The vast number of tree species presents a formidable challenge to understanding these forests, including their response to environmental change, as very little is known about most tropical tree species. A focus on the common species may circumvent this challenge. Here we investigate abundance patterns of common tree species using inventory data on 1,003,805 trees with trunk diameters of at least 10 cm across 1,568 locations1,2,3,4,5,6 in closed-canopy, structurally intact old-growth tropical forests in Africa, Amazonia and Southeast Asia. We estimate that 2.2%, 2.2% and 2.3% of species comprise 50% of the tropical trees in these regions, respectively. Extrapolating across all closed-canopy tropical forests, we estimate that just 1,053 species comprise half of Earth’s 800 billion tropical trees with trunk diameters of at least 10 cm. Despite differing biogeographic, climatic and anthropogenic histories7, we find notably consistent patterns of common species and species abundance distributions across the continents. This suggests that fundamental mechanisms of tree community assembly may apply to all tropical forests. Resampling analyses show that the most common species are likely to belong to a manageable list of known species, enabling targeted efforts to understand their ecology. Although they do not detract from the importance of rare species, our results open new opportunities to understand the world’s most diverse forests, including modelling their response to environmental change, by focusing on the common species that constitute the majority of their trees.Publisher PDFPeer reviewe