Enrichment and heating of the intracluster medium by ejection from galaxies

Results of N-body + hydrodynamic simulations designed to model the formation and evolution of clusters of galaxies and intracluster gas are presented. Clusters of galaxies are the largest bound, relaxed objects in the universe. They are strong x-ray emitters; this radiation originates through thermal bremsstrahlung from a diffuse plasma filling the space between cluster galaxies, the intracluster medium or ICM. From observations, one can infer that the mass of the ICM is comparable to or greater than the mass of all the galaxies in the cluster, and that the ratio of mass in hot gas to mass in galaxies, M(sub ICM)/M(sub STARS), increases with the richness of the cluster. Spectroscopic studies of cluster x-ray emission show heavy element emission lines. While M(sub ICM)/M(sub STARS) is greater than or equal to 1 implies that most of the ICM is primordial in nature, the discovery of heavy elements indicates that some of the gas must have been processed through galaxies. Galaxy evolution thus directly impacts cluster evolution

Weak Gravitational Lensing and Cluster Mass Estimates

Hierarchical theories of structure formation predict that clusters of galaxies should be embedded in a web like structure, with filaments emanating from them to large distances. The amount of mass contained within such filaments near a cluster can be comparable to the collapsed mass of the cluster itself. Diffuse infalling material also contains a large amount of mass. Both these components can contribute to the cluster weak lensing signal. This ``projection bias'' is maximized if a filament lies close to the line-of-sight to a cluster. Using large--scale numerical simulations of structure formation in a cosmological constant dominated cold dark matter model, we show that the projected mass typically exceeds the actual mass by several tens of percent. This effect is significant for attempts to estimate cluster masses through weak lensing observations, and will affect weak lensing surveys aimed at constructing the cluster mass function.Comment: 4 pages, 3 figures. LaTeX2e, uses emulateapj.sty and onecolfloat.sty. To be submitted to the Astrophysical Journal Letter

A field evaluation of the Hardy TB MODS Kit™ for the rapid phenotypic diagnosis of tuberculosis and multi-drug resistant tuberculosis.

BACKGROUND: Even though the WHO-endorsed, non-commercial MODS assay offers rapid, reliable TB liquid culture and phenotypic drug susceptibility testing (DST) at lower cost than any other diagnostic, uptake has been patchy. In part this reflects misperceptions about in-house assay quality assurance, but user convenience of one-stop procurement is also important. A commercial MODS kit was developed by Hardy Diagnostics (Santa Maria, CA, USA) with PATH (Seattle, WA, USA) to facilitate procurement, simplify procedures through readymade media, and enhance safety with a sealing silicone plate lid. Here we report the results from a large-scale field evaluation of the MODS kit in a government service laboratory. METHODS & FINDINGS: 2446 sputum samples were cultured in parallel in Lowenstein-Jensen (LJ), conventional MODS and in the MODS kit. MODS kit DST was compared with conventional MODS (direct) DST and proportion method (indirect) DST. 778 samples (31.8%) were Mycobacterium tuberculosis culture-positive. Compared to conventional MODS the sensitivity, specificity, positive, and negative predictive values (95% confidence intervals) of the MODS Kit were 99.3% (98.3-99.8%), 98.3% (97.5-98.8%), 95.8% (94.0-97.1%), and 99.7% (99.3-99.9%). Median (interquartile ranges) time to culture-positivity (and rifampicin and isoniazid DST) was 10 (9-13) days for conventional MODS and 8.5 (7-11) for MODS Kit (p<0.01). Direct rifampicin and isoniazid DST in MODS kit was almost universally concordant with conventional MODS (97.9% agreement, 665/679 evaluable samples) and reference indirect DST (97.9% agreement, 687/702 evaluable samples). CONCLUSIONS: MODS kit delivers performance indistinguishable from conventional MODS and offers a convenient, affordable alternative with enhanced safety from the sealing silicone lid. The availability in the marketplace of this platform, which conforms to European standards (CE-marked), readily repurposed for second-line DST in the near future, provides a fresh opportunity for improving equity of access to TB diagnosis and first and second-line DST in settings where the need is greatest

The Effect of the Cosmic Web on Cluster Weak Lensing Mass Estimates

In modern hierarchical theories of structure formation, rich clusters of galaxies form at the vertices of a weblike distribution of matter, with filaments emanating from them to large distances and with smaller objects forming and draining in along these filaments. The amount of mass contained in structure near the cluster can be comparable to the collapsed mass of the cluster itself. As the lensing kernel is quite broad along the line of sight around cluster lenses with typical redshifts near z=0.5, structures many Mpc away from the cluster are essentially at the same location as the cluster itself, when considering their effect on the cluster's weak lensing signal. We use large-scale numerical simulations of structure formation in a Lambda-dominated cold dark matter model to quantify the effect that large-scale structure near clusters has upon the cluster masses deduced from weak lensing analysis. A correction for the scatter in possible observed lensing masses should be included when interpreting mass functions from weak lensing surveys.Comment: 14 pages, 11 figures. LaTeX2e, uses emulateapj.sty and onecolfloat.st

The Ews-ERG Fusion Protein Can Initiate Neoplasia from Lineage-Committed Haematopoietic Cells

The EWS-ERG fusion protein is found in human sarcomas with the chromosomal translocation t(21;22)(q22;q12), where the translocation is considered to be an initiating event in sarcoma formation within uncommitted mesenchymal cells, probably long-lived progenitors capable of self renewal. The fusion protein may not therefore have an oncogenic capability beyond these progenitors. To assess whether EWS-ERG can be a tumour initiator in cells other than mesenchymal cells, we have analysed Ews-ERG fusion protein function in a cellular environment not typical of that found in human cancers, namely, committed lymphoid cells. We have used Ews-ERG invertor mice having an inverted ERG cDNA cassette flanked by loxP sites knocked in the Ews intron 8, crossed with mice expressing Cre recombinase under the control of the Rag1 gene to give conditional, lymphoid-specific expression of the fusion protein. Clonal T cell neoplasias arose in these mice. This conditional Ews gene fusion model of tumourigenesis shows that Ews-ERG can cause haematopoietic tumours and the precursor cells are committed cells. Thus, Ews-ERG can function in cells that do not have to be pluripotent progenitors or mesenchymal cells

Differences in white matter detected by ex vivo 9.4T MRI are associated with axonal changes in the R6/1 model of Huntington’s Disease

White matter (WM) volume loss has been reported in people with Huntington’s disease (HD), but the cellular basis of this deficit remains to be elucidated. To address this, we assessed ex vivo WM microstructure in the transgenic R6/1 mouse model of HD with magnetic resonance imaging (MRI) and studied the neurobiological basis of the MRI brain signals with histological and electron microscopy analyses in a separate cohort of age- and sex-matched mice. Differences in the macromolecular proton fraction (MPF) from quantitative magnetization transfer (qMT) as a proxy myelin measure, and the intra-axonal signal fraction (FR) from the composite hindered and restricted model of diffusion (CHARMED) as a proxy marker of axon density, were assessed alongside diffusion tensor imaging (DTI) parameters. A tractometry approach was employed to inspect region-specific differences across the corpus callosum (CC). Furthermore, voxel-based morphometry (VBM) and tract-based spatial statistics (TBSS) were used to explore brain-wise WM macro- and microstructure abnormalities. To gain insight into disease-associated impairments in attentional and visuospatial processing, a third cohort of age-matched mice was assessed with the 5-choice serial reaction time task (5-CSRTT). We report cognitive impairments in R6/1 mice and, by evaluating MRI and light and electron microscopy results, we show that this HD mouse model presents disruptions in axonal morphology (i.e. less complex, thinner axons) and organization (i.e. more densely packed axons). Furthermore, we show that, at least early in disease progression, R6/1 mice present a reduction in the expression or content of myelin-associated proteins without significant alterations in the structure of myelin sheaths. Finally, our findings indicate that neuroinflammation-driven glial and axonal swelling might also affect this mouse model early in disease progression. Crucially, we demonstrate the potential of FR, an in vivo estimate of axon density, as a novel MRI biomarker of HD-associated changes in WM microstructure

The Canadian Cluster Comparison Project: weak lensing masses and SZ scaling relations

The Canadian Cluster Comparison Project is a comprehensive multi-wavelength survey targeting 50 massive X-ray selected clusters of galaxies to examine baryonic tracers of cluster mass and to probe the cluster-to-cluster variation in the thermal properties of the hot intracluster medium. In this paper we present the weak lensing masses, based on the analysis of deep wide-field imaging data obtained using the Canada-France-Hawaii-Telescope. The final sample includes two additional clusters that were located in the field-of-view. We take these masses as our reference for the comparison of cluster properties at other wavelengths. In this paper we limit the comparison to published measurements of the Sunyaev-Zel'dovich effect. We find that this signal correlates well with the projected lensing mass, with an intrinsic scatter of 12\pm5% at ~r_2500, demonstrating it is an excellent proxy for cluster mass.Comment: 14 pages, 5 figures, submitted to MNRA

A spontaneous mutation in MutL-Homolog 3 (HvMLH3) affects synapsis and crossover resolution in the barley desynaptic mutant des10

Although meiosis is evolutionarily conserved, many of the underlying mechanisms show species-specific differences. These are poorly understood in large genome plant species such as barley (Hordeum vulgare) where meiotic recombination is very heavily skewed to the ends of chromosomes. The characterization of mutant lines can help elucidate how recombination is controlled. We used a combination of genetic segregation analysis, cytogenetics, immunocytology and 3D imaging to genetically map and characterize the barley meiotic mutant DESYNAPTIC 10 (des10). We identified a spontaneous exonic deletion in the orthologue of MutL-Homolog 3 (HvMlh3) as the causal lesion. Compared with wild-type, des10 mutants exhibit reduced recombination and fewer chiasmata, resulting in the loss of obligate crossovers and leading to chromosome mis-segregation. Using 3D structured illumination microscopy (3D-SIM), we observed that normal synapsis progression was also disrupted in des10, a phenotype that was not evident with standard confocal microscopy and that has not been reported with Mlh3 knockout mutants in Arabidopsis. Our data provide new insights on the interplay between synapsis and recombination in barley and highlight the need for detailed studies of meiosis in nonmodel species. This study also confirms the importance of early stages of prophase I for the control of recombination in large genome cereals.Isabelle Colas, Malcolm Macaulay, James D. Higgins, Dylan Phillips, Abdellah Barakate ... Robbie Waugh ... et al

Assessment of Select Climate Change Impacts on U.S. National Security

This report examines climate change impacts to U.S. national security by quantifying select impacts globally at the national level and identifying countries that are both at high risk from projected climate change and possess risk factors associated with political instability. Exposure to global sea‐level rise risk exposure is quantified by identifying low‐elevation coastal zones (LECZ), at 1, 3, 5, 7, 9, 10 and 12 meters of elevation. Countries with high risk factors for instability that also have the most people exposed to sea‐level rise include China, Philippines, India, and Indonesia. Those with the greatest percentage of population so exposed include Philippines, Egypt, and Indonesia. Within these countries, Egypt has especially high rates of population growth within the LECZ. Aggregate climate change vulnerability is quantified by using an index that takes into account both projected temperature change and adaptive capacity. For countries with high risk factors for instability, the most vulnerable countries are South Africa, Nepal, Morocco, Bangladesh, Tunisia, Paraguay, Yemen, Sudan and Côte d’Ivoire. Water scarcity is examined by comparing numbers of people living under conditions of water in the present with three future scenarios – one in which the climate remains unchanged but population changes; one in which population changes but the climate remains static; and one in which both population and climate change. Countries with high risk factors for instability that are projected to have the biggest increases in water scarcity are Mozambique, Côte d’Ivoire, Nigeria, Iraq, Guatemala, Zimbabwe, Ethiopia, Somalia, China, Syria and Algeria