National Aeronautics and Space Administration (NASA) Environmental Control and Life Support (ECLS) Integrated Roadmap Development

Although NASA is currently considering a number of future human space exploration mission concepts, detailed mission requirements and vehicle architectures remain mostly undefined, making technology investment strategies difficult to develop and sustain without a top-level roadmap to serve as a guide. This paper documents the process and results of an effort to define a roadmap for Environmental Control and Life Support Systems (ECLSS) capabilities required to enhance the long-term operation of the International Space Station (ISS) as well as enable beyond-Low Earth Orbit (LEO) human exploration missions. Three generic mission types were defined to serve as a basis for developing a prioritized list of needed capabilities and technologies. Those are 1) a short duration micro-gravity mission; 2) a long duration microgravity mission; and 3) a long duration partial gravity (surface) exploration mission. To organize the effort, a functional decomposition of ECLSS was completed starting with the three primary functions: atmosphere, water, and solid waste management. Each was further decomposed into sub-functions to the point that current state-of-the-art (SOA) technologies could be tied to the sub-function. Each technology was then assessed by NASA subject matter experts as to its ability to meet the functional needs of each of the three mission types. When SOA capabilities were deemed to fall short of meeting the needs of one or more mission types, those gaps were prioritized in terms of whether or not the corresponding capabilities enable or enhance each of the mission types. The result was a list of enabling and enhancing capability needs that can be used to guide future ECLSS development, as well as a list of existing hardware that is ready to go for exploration-class missions. A strategy to fulfill those needs over time was then developed in the form of a roadmap. Through execution of this roadmap, the hardware and technologies intended to meet exploration needs will, in many cases, directly benefit the ISS operational capability, benefit the Multi-Purpose Crew Vehicle (MPCV), and guide long-term technology investments for longer duration missions

National Aeronautics and Space Administration (NASA) Environmental Control and Life Support (ECLS) Capability Roadmap Development for Exploration

NASA is considering a number of future human space exploration mission concepts. Although detailed requirements and vehicle architectures remain mostly undefined, near-term technology investment decisions need to be guided by the anticipated capabilities needed to enable or enhance the mission concepts. This paper describes a roadmap that NASA has formulated to guide the development of Environmental Control and Life Support Systems (ECLSS) capabilities required to enhance the long-term operation of the International Space Station (ISS) and enable beyond-Low Earth Orbit (LEO) human exploration missions. Three generic mission types were defined to serve as a basis for developing a prioritized list of needed capabilities and technologies. Those are 1) a short duration micro gravity mission; 2) a long duration transit microgravity mission; and 3) a long duration surface exploration mission. To organize the effort, ECLSS was categorized into three major functional groups (atmosphere, water, and solid waste management) with each broken down into sub-functions. The ability of existing, flight-proven state-of-the-art (SOA) technologies to meet the functional needs of each of the three mission types was then assessed. When SOA capabilities fell short of meeting the needs, those "gaps" were prioritized in terms of whether or not the corresponding capabilities enable or enhance each of the mission types. The resulting list of enabling and enhancing capability gaps can be used to guide future ECLSS development. A strategy to fulfill those needs over time was then developed in the form of a roadmap. Through execution of this roadmap, the hardware and technologies needed to enable and enhance exploration may be developed in a manner that synergistically benefits the ISS operational capability, supports Multi-Purpose Crew Vehicle (MPCV) development, and sustains long-term technology investments for longer duration missions. This paper summarizes NASA s ECLSS capability roadmap development process, findings, and recommendatio

Environmental Control and Life Support (ECLS) Integrated Roadmap Development

This white paper documents a roadmap for development of Environmental Control and Life Support (ECLS) Systems (ECLSS) capabilities required to enable beyond-Low Earth Orbit (LEO) Exploration missions. In many cases, the execution of this Exploration-based roadmap will directly benefit International Space Station (ISS) operational capability by resolving known issues and/or improving overall system reliability. In addition, many of the resulting products will be applicable across multiple Exploration elements such as Multi-Purpose Crew Vehicle (MPCV), Multi-Mission Space Exploration Vehicle (MMSEV), Deep Space Habitat (DSH), and Landers. Within the ECLS community, this white paper will be a unifying tool that will improve coordination of resources, common hardware, and technologies. It will help to align efforts to focus on the highest priority needs that will produce life support systems for future human exploration missions that will simply run in the background, requiring minimal crew interaction

Promoting Healthy Decision-Making via Natural Environment Exposure: Initial Evidence and Future Directions

Research within psychology and other disciplines has shown that exposure to natural environments holds extensive physiological and psychological benefits. Adding to the health and cognitive benefits of natural environments, evidence suggests that exposure to nature also promotes healthy human decision-making. Unhealthy decision-making (e.g., smoking, non-medical prescription opioid misuse) and disorders associated with lack of impulse control [e.g., tobacco use, opioid use disorder (OUD)], contribute to millions of preventable deaths annually (i.e., 6 million people die each year of tobacco-related illness worldwide, deaths from opioids from 2002 to 2017 have more than quadrupled in the United States alone). Impulsive and unhealthy decision-making also contributes to many pressing environmental issues such as climate change. We recently demonstrated a causal link between visual exposure to nature (e.g., forests) and improved self-control (i.e., decreased impulsivity) in a laboratory setting, as well as the extent to which nearby nature and green space exposure improves self-control and health decisions in daily life outside of the experimental laboratory. Determining the benefits of nearby nature for self-controlled decision-making holds theoretical and applied implications for the design of our surrounding environments. In this article, we synergize the overarching results of recent research endeavors in three domains including the effects of nature exposure on (1) general health-related decision-making, (2) health and decision-making relevant for application to addiction related processes (e.g., OUD), and (3) environmentally relevant decision-making. We also discuss key future directions and conclusions

RIG-I agonist SLR10 promotes macrophage M1 polarization during influenza virus infection

RationaleA family of short synthetic, triphosphorylated stem-loop RNAs (SLRs) have been designed to activate the retinoic-acid-inducible gene I (RIG-I) pathway and induce a potent interferon (IFN) response, which may have therapeutic potential. We investigated immune response modulation by SLR10. We addressed whether RIG-I pathway activation with SLR10 leads to protection of nonsmoking (NS) and cigarette smoke (CS)-exposed mice after influenza A virus (IAV) infection.MethodsMice were given 25 µg of SLR10 1 day before IAV infection. We compared the survival rates and host immune responses of NS and CS-exposed mice following challenge with IAV.ResultsSLR10 significantly decreased weight loss and increased survival rates in both NS and CS-exposed mice during IAV infection. SLR10 administration repaired the impaired proinflammatory response in CS-exposed mice without causing more lung injury in NS mice as assessed by physiologic measurements. Although histopathologic study revealed that SLR10 administration was likely to result in higher pathological scores than untreated groups in both NS and CS mice, this change was not enough to increase lung injury evaluated by lung-to-body weight ratio. Both qRT-PCR on lung tissues and multiplex immunoassay on bronchoalveolar lavage fluids (BALFs) showed that most IFNs and proinflammatory cytokines were expressed at lower levels in SLR10-treated NS mice than control-treaded NS mice at day 5 post infection (p.i.). Remarkably, proinflammatory cytokines IL-6, IL-12, and GM-CSF were increased in CS-exposed mice by SLR10 at day 5 p.i. Significantly, SLR10 elevated the ratio of the two chemokines (CXCL9 and CCL17) in BALFs, suggesting macrophages were polarized to classically activated (M1) status. In vitro testing also found that SLR10 not only stimulated human alveolar macrophage polarization to an M1 phenotype, but also reversed cigarette smoke extract (CSE)-induced M2 to M1 polarization.ConclusionsOur data show that SLR10 administration in mice is protective for both NS and CS-exposed IAV-infected mice. Mechanistically, SLR10 treatment promoted M1 macrophage polarization in the lung during influenza infection. The protective effects by SLR10 may be a promising intervention for therapy for infections with viruses, particularly those with CS-enhanced susceptibility to adverse outcomes

Gene expression profiling of human alveolar macrophages infected by B. anthracis spores demonstrates TNF-α and NF-κb are key components of the innate immune response to the pathogen

<p>Abstract</p> <p>Background</p> <p><it>Bacillus anthracis</it>, the etiologic agent of anthrax, has recently been used as an agent of bioterrorism. The innate immune system initially appears to contain the pathogen at the site of entry. Because the human alveolar macrophage (HAM) plays a key role in lung innate immune responses, studying the HAM response to <it>B. anthracis </it>is important in understanding the pathogenesis of the pulmonary form of this disease.</p> <p>Methods</p> <p>In this paper, the transcriptional profile of <it>B. anthracis </it>spore-treated HAM was compared with that of mock-infected cells, and differentially expressed genes were identified by Affymetrix microarray analysis. A portion of the results were verified by Luminex protein analysis.</p> <p>Results</p> <p>The majority of genes modulated by spores were upregulated, and a lesser number were downregulated. The differentially expressed genes were subjected to Ingenuity Pathway analysis, the Database for Annotation, Visualization and Integrated Discovery (DAVID) analysis, the Promoter Analysis and Interaction Network Toolset (PAINT) and Oncomine analysis. Among the upregulated genes, we identified a group of chemokine ligand, apoptosis, and, interestingly, keratin filament genes. Central hubs regulating the activated genes were TNF-α, NF-κB and their ligands/receptors. In addition to TNF-α, a broad range of cytokines was induced, and this was confirmed at the level of translation by Luminex multiplex protein analysis. PAINT analysis revealed that many of the genes affected by spores contain the binding site for c-Rel, a member of the NF-κB family of transcription factors. Other transcription regulatory elements contained in many of the upregulated genes were c-Myb, CP2, Barbie Box, E2F and CRE-BP1. However, many of the genes are poorly annotated, indicating that they represent novel functions. Four of the genes most highly regulated by spores have only previously been associated with head and neck and lung carcinomas.</p> <p>Conclusion</p> <p>The results demonstrate not only that TNF-α and NF-κb are key components of the innate immune response to the pathogen, but also that a large part of the mechanisms by which the alveolar macrophage responds to <it>B. anthracis </it>are still unknown as many of the genes involved are poorly annotated.</p

The International Space Station (ISS) Port 1 (P1) External Active Thermal Control System (EATCS) Ammonia Leak

From 2011 to 2017, the crew onboard the International Space Station (ISS) was at risk of dire consequences due to an external ammonia leak. Ammonia is used in the External Active Thermal Control System (EATCS) to cool the pressurized modules and external electrical systems. Engineers at NASA's Johnson Space Center (JSC) initially detected the leak in one of two cooling loops by monitoring the system ammonia inventory decay over time. White flakes seen on High Definition (HD) cameras were also thought to be associated with the leakage but not confirmed. Initially, the leak was small enough that the ammonia inventory and system operations were not in jeopardy. However, the leak began to accelerate to the point where troubleshooting and corrective action were vital to the sustainability of the ISS. Therefore, it became imperative that the leak be located and repaired for ISS operations to continue. No tools were readily available on the ISS to locate such a leak when it was initially detected, however NASA engineers were already in the process of developing a new device for this purpose called the Robotic External Leak Locator (RELL). The RELL is a robotic instrument package with a mass spectrometer and an ion pressure gauge. Initial checkout operations with RELL happened to coincide with the increasing leak, and ammonia vapors were measured around the P1 EATCS Radiator #3 flexible jumper hoses. The leak stopped after the radiator and its flexible hoses were remotely isolated from the loop and the ammonia from the isolated segment was vented to space. Astronauts conducted a spacewalk that successfully removed the hoses, which were returned to ground for further investigation. The purpose of this paper is to review the leak detection and isolation efforts, investigation results, lessons learned and the recovery plan

The International Space Station (ISS) Port 1 (P1) External Active Thermal Control System (EATCS) Ammonia Leak

Ammonia is used in the Starboard 1 (S1) and Port 1 (P1) External Active Thermal Control System (EATCS) to cool the pressurized modules, and some of the external electrical power distribution hardware. Leaks that develop in these critical cooling systems that deplete in-line tanks can ultimately result in loss of cooling, which can have devastating impacts to the mission, science and crew onboard the ISS. A slow ammonia leak was initially observed from the P1 EATCS in 2011, but later in 2013 the leak rate began to accelerate. The ammonia inventory eventually began to decay exponentially, raising concerns that the inventory could drop to levels where the system would not be operational.The Robotic External Leak Locator (RELL) was built and launched to the ISS to detect and help locate ammonia leaks using the ISS Robotic Arm and remote ground operator control without constant crew involvement. RELL pinpointed the ammonia leak to the two flexible jumper hose assemblies connecting one of two fluid loops in one of the three deployable radiators to the P1 EATCS. The ammonia inside the two hose assemblies and that radiator fluid loop was isolated and vented to space in 2017. This stopped the leak and an Extravehicular Activity was conducted to remove the two hose assemblies so they could be returned to ground for further Test, Teardown and Evaluation (TT&E). The purpose of this presentation is to discuss this leakage scenario and the TT&E efforts

Stem Cell Emergence and Hemopoietic Activity Are Incompatible in Mouse Intraembryonic Sites

In the mouse embryo, the generation of candidate progenitors for long-lasting hemopoiesis has been reported in the paraaortic splanchnopleura (P-Sp)/aorta-gonad-mesonephros (AGM) region. Here, we address the following question: can the P-Sp/AGM environment support hemopoietic differentiation as well as generate stem cells, and, conversely, are other sites where hemopoietic differentiation occurs capable of generating stem cells? Although P-Sp/AGM generates de novo hemopoietic stem cells between 9.5 and 12.5 days post coitus (dpc), we show here that it does not support hemopoietic differentiation. Among mesoderm-derived sites, spleen and omentum were shown to be colonized by exogenous cells in the same fashion as the fetal liver. Cells colonizing the spleen were multipotent and pursued their evolution to committed progenitors in this organ. In contrast, the omentum, which was colonized by lymphoid-committed progenitors that did not expand, cannot be considered as a hemopoietic organ. From these data, stem cell generation appears incompatible with hemopoietic activity. At the peak of hemopoietic progenitor production in the P-Sp/AGM, between 10.5 and 11.5 dpc, multipotent cells were found at the exceptional frequency of 1 out of 12 total cells and 1 out of 4 AA4.1+ cells. Thus, progenitors within this region constitute a pool of undifferentiated hemopoietic cells readily accessible for characterization

New views on the emission and structure of the solar transition region

The Sun is the only star that we can spatially resolve and it can be regarded as a fundamental plasma laboratory of astrophysics. The solar transition region (TR), the layer between the solar chromosphere and corona, plays an important role in solar wind origin and coronal heating. Recent high-resolution observations made by SOHO, TRACE, and Hinode indicate that the TR is highly nonuniform and magnetically structured. Through a combination of spectroscopic observations and magnetic field extrapolations, the TR magnetic structures and plasma properties have been found to be different in coronal holes and in the quiet Sun. In active regions, the TR density and temperature structures also differ in sunspots and the surrounding plage regions. Although the TR is believed to be a dynamic layer, quasi-steady flows lasting from several hours to several days are often present in the quiet Sun, coronal holes, and active regions, indicating some kind of plasma circulation/convection in the TR and corona. The emission of hydrogen Lyman lines, which originates from the lower TR, has also been intensively investigated in the recent past. Observations show clearly that the flows and dynamics in the middle and upper TR can greatly modify the Lyman line profiles.Comment: This paper has been withdrawn by the authors. This is a repetition of another record in ADS: New Astronomy Reviews, Volume 54, Issue 1-2, p. 13-3