Effects of Thyroxine Exposure on Osteogenesis in Mouse Calvarial Pre-Osteoblasts

The incidence of craniosynostosis is one in every 1,800-2500 births. The gene-environment model proposes that if a genetic predisposition is coupled with environmental exposures, the effects can be multiplicative resulting in severely abnormal phenotypes. At present, very little is known about the role of gene-environment interactions in modulating craniosynostosis phenotypes, but prior evidence suggests a role for endocrine factors. Here we provide a report of the effects of thyroid hormone exposure on murine calvaria cells. Murine derived calvaria cells were exposed to critical doses of pharmaceutical thyroxine and analyzed after 3 and 7 days of treatment. Endpoint assays were designed to determine the effects of the hormone exposure on markers of osteogenesis and included, proliferation assay, quantitative ALP activity assay, targeted qPCR for mRNA expression of Runx2, Alp, Ocn, and Twist1, genechip array for 28,853 targets, and targeted osteogenic microarray with qPCR confirmations. Exposure to thyroxine stimulated the cells to express ALP in a dose dependent manner. There were no patterns of difference observed for proliferation. Targeted RNA expression data confirmed expression increases for Alp and Ocn at 7 days in culture. The genechip array suggests substantive expression differences for 46 gene targets and the targeted osteogenesis microarray indicated 23 targets with substantive differences. 11 gene targets were chosen for qPCR confirmation because of their known association with bone or craniosynostosis (Col2a1, Dmp1, Fgf1, 2, Igf1, Mmp9, Phex, Tnf, Htra1, Por, and Dcn). We confirmed substantive increases in mRNA for Phex, FGF1, 2, Tnf, Dmp1, Htra1, Por, Igf1 and Mmp9, and substantive decreases for Dcn. It appears thyroid hormone may exert its effects through increasing osteogenesis. Targets isolated suggest a possible interaction for those gene products associated with calvarial suture growth and homeostasis as well as craniosynostosis. © 2013 Cray et al

The Ross Sea Dipole-temperature, snow accumulation and sea ice variability in the Ross Sea region, Antarctica, over the past 2700 years

High-resolution, well-dated climate archives provide an opportunity to investigate the dynamic interactions of climate patterns relevant for future projections. Here, we present data from a new, annually dated ice core record from the eastern Ross Sea, named the Roosevelt Island Climate Evolution (RICE) ice core. Comparison of this record with climate reanalysis data for the 1979-2012 interval shows that RICE reliably captures temperature and snow precipitation variability in the region. Trends over the past 2700 years in RICE are shown to be distinct from those in West Antarctica and the western Ross Sea captured by other ice cores. For most of this interval, the eastern Ross Sea was warming (or showing isotopic enrichment for other reasons), with increased snow accumulation and perhaps decreased sea ice concentration. However, West Antarctica cooled and the western Ross Sea showed no significant isotope temperature trend. This pattern here is referred to as the Ross Sea Dipole. Notably, during the Little Ice Age, West Antarctica and the western Ross Sea experienced colder than average temperatures, while the eastern Ross Sea underwent a period of warming or increased isotopic enrichment. From the 17th century onwards, this dipole relationship changed. All three regions show current warming, with snow accumulation declining in West Antarctica and the eastern Ross Sea but increasing in the western Ross Sea. We interpret this pattern as reflecting an increase in sea ice in the eastern Ross Sea with perhaps the establishment of a modern Roosevelt Island polynya as a local moisture source for RICE

A 50,000-year record of lake-level variations and overflow from Owens Lake, eastern California, USA

A continuous lake-level curve was constructed for Owens Lake, eastern California by integrating lake-core data and shoreline geomorphology with new wind-wave and sediment entrainment modeling of lake-core sedimentology. This effort enabled refinement of the overflow history and development of a better understanding of the effects of regional and global climate variability on lake levels of the paleo-Owens River system during the last 50,000 years. The elevations of stratigraphic sites, plus lake bottom and spillway positions were corrected for vertical tectonic deformation using a differential fault-block model to estimate the absolute hydrologic change of the watershed-lake system. New results include 14C dating of mollusk shells in shoreline deposits, plus post-IR-IRSL dating of a suite of five beach ridges and OSL dating of spillway alluvial and deltaic deposits in deep boreholes. Geotechnical data show the overflow area is an entrenched channel that had erodible sills composed of unconsolidated fluvial-deltaic and alluvial sediment at elevations of ∼1113–1165 m above mean sea level. Owens Lake spilled most of the time at or near minimum sill levels, controlled by a bedrock sill at ∼1113 m. Nine major transgressions at ∼40.0, 38.7, 23.3, 19.3, 15.6, 13.8, 12.8, 11.6, and 10.6 ka reached levels ∼10–45 m above the bedrock sill. Several major regressions at or below the bedrock sill from 36.9 to 28.5 ka, and at ∼17.8, 12.9, and 10.4–8.8 ka indicate little to no overflow during these times. The latest period of overflow occurred ∼10–20 m above the bedrock sill from ∼8.4 to 6.4 ka that was followed by closed basin conditions after ∼6.4 ka. Previous lake core age-depth models were revised by accounting for sediment compaction and using no reservoir correction for open basin conditions, thereby reducing discrepancies between Owens Lake shoreline and lake-core proxy records. The integrated analysis provides a continuous 50 ka lake-level record of hydroclimate variability along the south-central Sierra Nevada that is consistent with other shoreline and speleothem records in the southwestern U.S

Evolution of mean ocean temperature in Marine Isotope Stages 5-4

Abstract. Deglaciations are characterized by relatively fast and near-synchronous changes in ice sheet volume, ocean temperature, and atmospheric greenhouse gas concentrations, but glacial inceptions occur more gradually. Understanding the evolution of ice sheet, ocean, and atmospheric conditions from interglacial to glacial maximum provides important insight into the interplay of these components of our climate system. Using noble gas measurements in ancient ice samples, we reconstruct mean ocean temperature (MOT) from 74 to 59.5 ka BP, covering the Marine Isotope Stage (MIS) 5-4 boundary, MIS 4, and part of the MIS 4-3 transition. Comparing this MOT reconstruction to previously published MOT reconstructions from the last glacial cycle, we find that the majority of interglacial-glacial ocean cooling occurred across MIS 5, and MOT reached full glacial levels by MIS 4 (−2.7 ± 0.3 °C relative to the Holocene). Comparing MOT to contemporaneous records of CO2 and benthic ?18O, we find that ocean cooling and the solubility pump can explain most of the CO2 drawdown and increase in ?18O across MIS 5. The timing of ocean warming and cooling in our record indicates that millennial scale climate variability plays a crucial role in setting mean ocean temperature during this interval, as seen during other periods, such as the last deglaciation

Evolution of mean ocean temperature in Marine Isotope Stage 4

Deglaciations are characterized by relatively fast and near-synchronous changes in ice sheet volume, ocean temperature, and atmospheric greenhouse gas concentrations, but glacial inception occurs more gradually. Understanding the evolution of ice sheet, ocean, and atmosphere conditions from interglacial to glacial maximum provides insight into the interplay of these components of the climate system. Using noble gas measurements in ancient ice samples, we reconstruct mean ocean temperature (MOT) from 74 to 59.7 ka, covering the Marine Isotope Stage (MIS) 5a–4 boundary, MIS 4, and part of the MIS 4–3 transition. Comparing this MOT reconstruction to previously published MOT reconstructions from the last and penultimate deglaciation, we find that the majority of the last interglacial–glacial ocean cooling must have occurred within MIS 5. MOT reached equally cold conditions in MIS 4 as in MIS 2 (−2.7 ± 0.3 ∘C relative to the Holocene, −0.1 ± 0.3 ∘C relative to MIS 2). Using a carbon cycle model to quantify the CO2 solubility pump, we show that ocean cooling can explain most of the CO2 drawdown (32 ± 4 of 40 ppm) across MIS 5. Comparing MOT to contemporaneous records of benthic δ18O, we find that ocean cooling can also explain the majority of the δ18O increase across MIS 5 (0.7 ‰ of 1.3 ‰). The timing of ocean warming and cooling in the record and the comparison to coeval Antarctic isotope data suggest an intimate link between ocean heat content, Southern Hemisphere high-latitude climate, and ocean circulation on orbital and millennial timescales.</p