The association of polymorphic alleles of folate metabolism genes with ivf and et failures

The purpose of the study was to determine the effects of polymorphic variants of folate cycle genes with pregnancy rate in IVF and ET protocols.Materials and methods: a comparative is satisfied an open prospective cohort study in 87 patients with IVF failure and 46 patients, who became pregnant and gave birth after IVF and ET. All patients performed a survey of genetic polymorphisms of MTHFR C677T, MTR A2756G and MTRR A66G genes. Results. Revealed that patients in the group with IVF failed statistically significant increase in the proportion of polymorphic allele 677T (p = 0,030, OR -1,71 (0,96-3,03)), and allele 677T with allele 66G combination (p = 0,049, OR -1,86 (0,87-3,96)).Conclusions. The presence of homozygous or heterozygous form of mutation of the gene MTHFR (genotype 677C/T and 677ТЯ), as well as any form of combination of this mutation with a mutation of the gene MTRR (genotype 66A/G, 66G/G) is associated with a reduction in the effectiveness of IVF and ET.Цель работы заключалась в определении влияния полиморфных вариантов генов фолатного цикла на частоту наступления беременности в протоколе ЭКО и ПЭ. Материалы и методы: выполнено открытое сравнительное проспективное когортное исследование 87 пациенток с неудачами ЭКО и ПЭ и 46 пациенток, забеременевших и родивших в результате ЭКО и ПЭ. Всем пациенткам выполнено обследование на генетические полиморфизмы генов MTHFR (С677Т), MTR (A2756G), MTRR (A66G). Результаты. Выявлено, что у пациенток из группы с неудачной попыткой ЭКО и ПЭ отмечается достоверное увеличение доли полиморфного аллеля 677Т (р=0,030, OR -1,71 (0,96-3,03)), а так же увеличение доли сочетания аллеля 677Т с аллелем 66G (р=0,049. OR - 1,86 (0,87-3,96)).Выводы. Наличие гомозиготной или гетерозиготной формы мутации гена MTHFR (генотип 677СЯ и 677ТЯ), а так же сочетание любой формы данной мутации с мутацией гена MTRR (генотип 66A/G, 66G/G) ассоциировано со снижением эффективности программы ЭКО и ПЭ

The Influence of Socio-Cultural Factors on Oocyte Donors’ Motivations and Disclosure Decisions

Received 9 June 2022 © 2022. Accepted 23 September 2022. Published online 10 October 2022.The study focuses on the motivations of Russian oocyte donors and the socio-cultural factors affecting these motivations. We conducted 16 semi-structured interviews with actual or prospective oocyte donors. All of them were patients of two fertility clinics located in the city of Yekaterinburg (Russia) planning to become oocyte donors within the period from 2 weeks to 3 months. We built a profile of a Russian oocyte donor: it is a 26-year-old married woman with at least one child, she has a secondary vocational education and a low income. All the women in our study displayed multiple motivations: apart from the interest in a financial reward and purely altruistic motivations, for many women the decisive factor is their desire to help their friends or relatives struggling with infertility. Interestingly, almost all of our respondents described their decision to donate as an attempt to move past a traumatic situation they once endured and to achieve closure by doing something really important and good. For many women, oocyte donation becomes a way to boost their self-esteem, to feel more significant and to promote their personal autonomy. As for barriers to donation, one of the most important is associated with the donors’ unwillingness to make their identity known and to share this information even with their close circle of friends and relatives. For the majority of donors, anonymity is crucial. The disclosure of this information, in their view, will cause controversy in the donor’s family and immediate circle of friends at present and will threaten their privacy in the future. The prospective donors are also concerned about the negative public attitudes or lack of understanding.The study was conducted within the framework of the RSF grant No. 22-28-01694 dated December 20, 2021

Geometric estimation of volcanic eruption column height from GOES-R near-limb imagery-Part 2: Case studies

In a companion paper (Horváth et al., 2021), we introduced a new technique to estimate volcanic eruption column height from extremely oblique near-limb geostationary views. The current paper demonstrates and validates the technique in a number of recent eruptions, ranging from ones with weak columnar plumes to subplinian events with massive umbrella clouds and overshooting tops that penetrate the stratosphere. Due to its purely geometric nature, the new method is shown to be unaffected by the limitations of the traditional brightness temperature method, such as height underestimation in subpixel and semitransparent plumes, ambiguous solutions near the tropopause temperature inversion, or the lack of solutions in undercooled plumes. The side view height estimates were in good agreement with plume heights derived from ground-based video and satellite stereo observations, suggesting they can be a useful complement to established techniques

Endothelial function, regulation of angiogenesis and embryonic central hemodynamics in ART-conceived pregnancies

This study was undertaken to compare the concentrations of pro- and anti-angiogenic growth factors, nitric oxide (NO) stable metabolites in maternal serum and embryonic left ventricular (LV) isovolumic relaxation time (IRT, ms) during the first trimester in two groups of women: with pregnancy conceived by assisted reproductive technologies (ART, nј39) and normally conceived (control group, nј68) pregnancy. The concentration of vasoconstrictor endothelin 1 was 45.5 times more in ART than in control group. On the contrary, the concentrations of NO stable metabolites in ART were 1.9 times less than in control women. The assessment of angiogenic suppressors in ART women demonstrates the decrease in s-endoglin concentration was 1.6 times and in soluble receptor to vascular endothelial growth factor concentration was 2.0 times in comparison with control group. There was a significant increase in LV IRT in ART embryos in comparison to control ones. These data suggest significant changes in pro-antiangiogenic factors balance and increase in vascular impedance in ART-conceived embryos

Prognosis treatment exodus parturients with multiple organ failure

Objective: То study the dynamics on the basis of the basic parameters of homeostasis in patients with multiple organ failure after complicated delivery to identify the most informative clinical and laboratory parameters that predict treatment outcome. Material and Methods: a retrospective cohort study that included 173 women with multiple organ failure after complicated delivery. Divided into 3 groups: group 1 (n = 86) - the surviving patients with intact uterus, group 2 (n = 67) - the surviving patients who had undergone hysterectomy; third group (n = 20) - the dead woman in childbirth. Assessment of patients was carried out on set of clinical and laboratory signs, according to standard research in resuscitation and intensive care admission (2-3 days after birth) on the 5th day after birth and on the 10th day. Results and discussion. Noteworthy significantly shorter period of delivery patients, who subsequently managed to maintain reproductive function (33,86 + 0,56 weeks.). Women with a favorable outcome later, less time spent in hospitals 1 and 2 levels (2,88 + 0,37). Patients of group 1 had a lesion on the average of 2 systems, two groups of woman in childbirth - 3 systems, and subsequently died (group 3) – 4 systems. In the study of the basic parameters of homeostasis in parturients dynamics have been identified and formulated criteria for informative decision rule forecast preservation of reproductive function, which can guide the work of practitioners.Цель работы: на основании изучения динамики основных параметров гомеостаза у пациенток с полиорганной недостаточностью после осложненных родов выявить наиболее информативные клинико-лабораторные показатели, позволяющие прогнозировать исход лечения. Материал и методы: проведено ретроспективное когортное исследование, в которое были включены 173 женщины с полиорганной недостаточностью после осложненных родов. Выделены 3 группы: 1-я группа (п=86)- выжившие пациентки с сохраненной маткой: 2-я группа (п=67)- выжившие пациентки, перенесшие гистерэктомию; 3-я группа (п=20)- умершие родильницы. Оценка состояния пациенток проводилась по совокупности клиниколабораторных признаков, согласно стандарту исследования в отделениях реанимации и интенсивной терапии при поступлении (2-3 сутки после родов), на 5-е сутки после родов и на 10-е сутки. Результаты и обсуждение. Обращает на себя внимание достоверно меньший срок родоразрешения пациенток, у которых в дальнейшем удалось сохранить репродуктивную функцию (33,86+0,56 нед.). Женщины, с благоприятным исходом впоследствии, меньшее время пробыли в ЛПУ 1 и 2 уровня (2,88+0,37). Пациентки 1 группы имели поражение в среднем 2-х систем, родильницы 2 группы - 3-х систем, а умершие впоследствии (3 группа) - 4-х систем. При исследовании основных параметров гомеостаза родильниц в динамике были выявлены информативные критерии и сформулировано решающее правило прогноза сохранения репродуктивной функции, которым могут руководствоваться в своей работе практические врачи

Measurement of the CKM angle γ from a combination of B±→Dh± analyses

A combination of three LHCb measurements of the CKM angle γ is presented. The decays B±→D K± and B±→Dπ± are used, where D denotes an admixture of D0 and D0 mesons, decaying into K+K−, π+π−, K±π∓, K±π∓π±π∓, K0Sπ+π−, or K0S K+K− final states. All measurements use a dataset corresponding to 1.0 fb−1 of integrated luminosity. Combining results from B±→D K± decays alone a best-fit value of γ =72.0◦ is found, and confidence intervals are set γ ∈ [56.4,86.7]◦ at 68% CL, γ ∈ [42.6,99.6]◦ at 95% CL. The best-fit value of γ found from a combination of results from B±→Dπ± decays alone, is γ =18.9◦, and the confidence intervals γ ∈ [7.4,99.2]◦ ∪ [167.9,176.4]◦ at 68% CL are set, without constraint at 95% CL. The combination of results from B± → D K± and B± → Dπ± decays gives a best-fit value of γ =72.6◦ and the confidence intervals γ ∈ [55.4,82.3]◦ at 68% CL, γ ∈ [40.2,92.7]◦ at 95% CL are set. All values are expressed modulo 180◦, and are obtained taking into account the effect of D0–D0 mixing

Differential branching fraction and angular analysis of the decay B0→K∗0μ+μ−

The angular distribution and differential branching fraction of the decay B 0→ K ∗0 μ + μ − are studied using a data sample, collected by the LHCb experiment in pp collisions at s√=7 TeV, corresponding to an integrated luminosity of 1.0 fb−1. Several angular observables are measured in bins of the dimuon invariant mass squared, q 2. A first measurement of the zero-crossing point of the forward-backward asymmetry of the dimuon system is also presented. The zero-crossing point is measured to be q20=4.9±0.9GeV2/c4 , where the uncertainty is the sum of statistical and systematic uncertainties. The results are consistent with the Standard Model predictions

Measurement of the ratio of branching fractions BR(B0 -> K*0 gamma)/BR(Bs0 -> phi gamma)

The ratio of branching fractions of the radiative B decays B0 -> K*0 gamma and Bs0 -> phi gamma has been measured using 0.37 fb-1 of pp collisions at a centre of mass energy of sqrt(s) = 7 TeV, collected by the LHCb experiment. The value obtained is BR(B0 -> K*0 gamma)/BR(Bs0 -> phi gamma) = 1.12 +/- 0.08 ^{+0.06}_{-0.04} ^{+0.09}_{-0.08}, where the first uncertainty is statistical, the second systematic and the third is associated to the ratio of fragmentation fractions fs/fd. Using the world average for BR(B0 -> K*0 gamma) = (4.33 +/- 0.15) x 10^{-5}, the branching fraction BR(Bs0 -> phi gamma) is measured to be (3.9 +/- 0.5) x 10^{-5}, which is the most precise measurement to date.Comment: 15 pages, 1 figure, 2 table

Measurement of the relative rate of prompt χc0, χc1 and χc2 production at √s=7TeV

Prompt production of charmonium χc0, χc1 and χc2 mesons is studied using proton-proton collisions at the LHC at a centre-of-mass energy of √s=7TeV. The χc mesons are identified through their decay to J/ψγ, with J/ψ→μ+mu− using photons that converted in the detector. A data sample, corresponding to an integrated luminosity of 1.0fb−1 collected by the LHCb detector, is used to measure the relative prompt production rate of χc1 and χc2 in the rapidity range 2.0<y<4.5 as a function of the J/ψ transverse momentum from 3 to 20 GeV/c. First evidence for χc0 meson production at a hadron collider is also presented