WordMelodies: supporting children with visual impairment in learning literacy

We present WordMelodies, an inclusive, cross platform, mobile app that supports children with visual impairments in the acquisition of basic literacy skills through 8 different exercises. WordMelodies has been designed and evaluated by three domain experts in assistive technologies and education for children with visual impairments. After three design and evaluation iterations the app is fully accessible, except for one limitation of the cross platform development toolkit used

SmartWheels: Detecting urban features for wheelchair users’ navigation

People with mobility impairments have heterogeneous needs and abilities while moving in an urban environment and hence they require personalized navigation instructions. Providing these instructions requires the knowledge of urban features like curb ramps, steps or other obstacles along the way. Since these urban features are not available from maps and change in time, crowdsourcing this information from end-users is a scalable and promising solution. However, it is inconvenient for wheelchair users to input data while on the move. Hence, an automatic crowdsourcing mechanism is needed. In this contribution we present SmartWheels, a solution to detect urban features by analyzing inertial sensors data produced by wheelchair movements. Activity recognition techniques are used to process the sensors data stream. SmartWheels is evaluated on data collected from 17 real wheelchair users navigating in a controlled environment (10 users) and in-the-wild (7 users). Experimental results show that SmartWheels is a viable solution to detect urban features, in particular by applying specific strategies based on the confidence assigned to predictions by the classifier

Trace elements in stomach oil of Scopoli's shearwater (Calonectris diomedea) from Linosa's colony

Calonectris diomedea is a colonial Procellariiform breeding on Mediterranean islands. The stomach oil produced during chick rearing is a peculiar trait of this species. The composition of the stomach oil is likely to reflect the composition of the prey ingested and might reveal the contaminants uptake with prey becoming a possible tool for the marine pollution monitoring. We examined the concentration of 15 trace elements by ICP-MS and direct mercury analyser. The principal component analysis revealed a heterogeneous pattern of metal concentration, showing a significant separation between samples collected 20 and 70 days after hatching. The data obtained in this work give preliminary information on the feeding habits and breeding ecology of Linosa's colony of Scopoli's shearwater. The trace metals variability found suggest that the stomach oil may have a role as trophic markers to understand predator-prey relationships and to have evidence on the accumulation of pollutants in the latter

The NATO project : nanoparticle-based countermeasures for microgravity-induced osteoporosis

Recent advances in nanotechnology applied to medicine and regenerative medicine have an enormous and unexploited potential for future space and terrestrial medical applications. The Nanoparticles and Osteoporosis (NATO) project aimed to develop innovative countermeasures for secondary osteoporosis affecting astronauts after prolonged periods in space microgravity. Calcium- and Strontium-containing hydroxyapatite nanoparticles (nCa-HAP and nSr-HAP, respectively) were previously developed and chemically characterized. This study constitutes the first investigation of the effect of the exogenous addition of nCa-HAP and nSr-HAP on bone remodeling in gravity (1\u2009g), Random Positioning Machine (RPM) and onboard International Space Station (ISS) using human bone marrow mesenchymal stem cells (hBMMSCs). In 1\u2009g conditions, nSr-HAP accelerated and improved the commitment of cells to differentiate towards osteoblasts, as shown by the augmented alkaline phosphatase (ALP) activity and the up-regulation of the expression of bone marker genes, supporting the increased extracellular bone matrix deposition and mineralization. The nSr-HAP treatment exerted a protective effect on the microgravity-induced reduction of ALP activity in RPM samples, and a promoting effect on the deposition of hydroxyapatite crystals in either ISS or 1\u2009g samples. The results indicate the exogenous addition of nSr-HAP could be potentially used to deliver Sr to bone tissue and promote its regeneration, as component of bone substitute synthetic materials and additive for pharmaceutical preparation or food supplementary for systemic distribution

Synchronized onset of nuclear and cell surface modifications in U937 cells during apoptosis

In this study we investigated the relationship between nuclear and cell surface modifications (i.e. blebbing, phosphatidylserine [PS] and sugar residues exposure) in a monocytic cell line, U937, during apoptosis induced by oxidative stress (1mM H2O2) or inhibition of protein synthesis (10 mg/ml puromycin). Dying cells were simultaneously observed for nuclear modifications, presence of superficial blebs and plasma membrane alterations. Morphological analysis performed by conventional fluorescence microscopy, or by transmission and scanning electron microscopy showed that the courses of nuclear and membrane alterations occured concomitantly, but the phenotype was dependent on the stage of the apoptotic process and the type of apoptogenic inducer used. The progression of apoptosis in U937 cells beyond early stages resulted in the extensive formation of blebs which concomitantly lost some typical markers of apoptosis, such as PS and sugar residues. Therefore, the modality by which the nucleus condenses, or the amount and the pattern of distribution of PS on the cell surface were, for each cell line, strictly related to the apoptogenic inducer. The morphological data reported in the present paper should lead to a more precise quantification of apoptosis by improving the detection of apoptotic cells in vivo (i.e. in tissue, organs), which is a crucial point in the evaluation of efficiency of antiproliferative drugs, such as antiblastic or immunosuppressive compounds

Robust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells.

The epicardium has emerged as a multipotent cardiovascular progenitor source with therapeutic potential for coronary smooth muscle cell, cardiac fibroblast (CF) and cardiomyocyte regeneration, owing to its fundamental role in heart development and its potential ability to initiate myocardial repair in injured adult tissues. Here, we describe a chemically defined method for generating epicardium and epicardium-derived smooth muscle cells (EPI-SMCs) and CFs from human pluripotent stem cells (HPSCs) through an intermediate lateral plate mesoderm (LM) stage. HPSCs were initially differentiated to LM in the presence of FGF2 and high levels of BMP4. The LM was robustly differentiated to an epicardial lineage by activation of WNT, BMP and retinoic acid signalling pathways. HPSC-derived epicardium displayed enhanced expression of epithelial- and epicardium-specific markers, exhibited morphological features comparable with human foetal epicardial explants and engrafted in the subepicardial space in vivo. The in vitro-derived epicardial cells underwent an epithelial-to-mesenchymal transition when treated with PDGF-BB and TGFβ1, resulting in vascular SMCs that displayed contractile ability in response to vasoconstrictors. Furthermore, the EPI-SMCs displayed low density lipoprotein uptake and effective lowering of lipoprotein levels upon treatment with statins, similar to primary human coronary artery SMCs. Cumulatively, these findings suggest that HPSC-derived epicardium and EPI-SMCs could serve as important tools for studying human cardiogenesis, and as a platform for vascular disease modelling and drug screening.This work was supported by the British Heart Foundation (BHF) [NH/11/1/28922], by the UK Medical Research Council and BHF [G1000847 to S.S. and R.A.P.), and by Cambridge Hospitals National Institute for Health Research Biomedical Research Centre funding (S.S. and R.A.P.). D.I. is on a University of Cambridge Commonwealth Scholarship. S.S. and F.S. are supported by the BHF [FS/13/29/ 30024]. L.G. is supported by the BHF [RM/l3/3/30159]. W.G.B. and V.L.M. are supported by BHF PhD studentships [FS/11/77/29327 and FS/10/48/28674].This is the final published version. It first appeared at http://dev.biologists.org/content/early/2015/03/25/dev.119271.abstract

Evidence for X-ray synchrotron emission from simultaneous mid-IR to X-ray observations of a strong Sgr A* flare

This paper reports measurements of Sgr A* made with NACO in L' -band (3.80 um), Ks-band (2.12 um) and H-band (1.66 um) and with VISIR in N-band (11.88 um) at the ESO VLT, as well as with XMM-Newton at X-ray (2-10 keV) wavelengths. On 4 April, 2007, a very bright flare was observed from Sgr A* simultaneously at L'-band and X-ray wavelengths. No emission was detected using VISIR. The resulting SED has a blue slope (beta > 0 for nuL_nu ~ nu^beta, consistent with nuL_nu ~ nu^0.4) between 12 micron and 3.8 micron. For the first time our high quality data allow a detailed comparison of infrared and X-ray light curves with a resolution of a few minutes. The IR and X-ray flares are simultaneous to within 3 minutes. However the IR flare lasts significantly longer than the X-ray flare (both before and after the X-ray peak) and prominent substructures in the 3.8 micron light curve are clearly not seen in the X-ray data. From the shortest timescale variations in the L'-band lightcurve we find that the flaring region must be no more than 1.2 R_S in size. The high X-ray to infrared flux ratio, blue nuL_nu slope MIR to L' -band, and the soft nuL_nu spectral index of the X-ray flare together place strong constraints on possible flare emission mechanisms. We find that it is quantitatively difficult to explain this bright X-ray flare with inverse Compton processes. A synchrotron emission scenario from an electron distribution with a cooling break is a more viable scenario.Comment: ApJ, 49 pages, 9 figure