Weather-based models for predicting grape powdery mildew (Uncinula necator (Schwein) Burrill) epidemics

El oídio de la vid (Vitis vinifera L.), causado por Uncinula necator (Schwein) Burrill, es una enfermedad fúngica relevante en viñedos de la provincia de Mendoza, ocasionando pérdidas de producción y degradación de la calidad del fruto cosechado. Durante seis campañas agrícolas (2001/02; 2002/03; 2005/06; 2007/08; 2008/09; 2009/10), en un parral del cultivar susceptible Chenín (EEA INTA Mendoza, Luján de Cuyo), se observó la evolución de la incidencia del oídio en racimos (como tasa de incremento epidémica diaria), desde floración hasta envero. A partir de registros térmico-hídricos horarios (monitoreados con sensores ubicados en la parte superior del canopeo) se calcularon variables meteorológicas en los 15 días previos a cada observación del progreso de la epidemia. Con variables que integran efectos de la temperatura, humedad del aire y frecuencia de precipitaciones ligeras sobre el incremento de la enfermedad en función del tiempo, se ajustaron modelos de regresión logística para estimar las probabilidades de ocurrencia de tasas epidémicas categorizadas (severa, moderada y nula), logrando una precisión de predicción máxima de 92,5% (modelo bivariado, N=40). Las predicciones del modelo univariado (seleccionado por "Stepwise"; precisión de predicción: 87,5%) se validaron satisfactoriamente con la curva epidémica observada en 2000/2001. Los valores estimados por estos modelos podrían complementar a las observaciones epidémicas y emitir alarmas regionales que sustenten la toma de decisión de control químico.Powdery mildew, caused by Uncinula necator (Schwein) Burrill is one of the most damaging disease affecting grapevines (Vitis vinifera L.) in Mendoza and worldwide, reducing yield and fruit quality. Throughout six growing seasons (2001/02; 2002/03; 2005/06; 2007/08; 2008/09; 2009/10), powdery mildew incidence values (expressed as daily epidemic increment rates) were observed in clusters from flowering to onset of ripening (change of color of the grape berries), in a grapevine planted with the susceptible Chenin cultivar at the EEA INTA Mendoza (Luján de Cuyo). From hourly values of thermal-moisture elements recorded by upper canopy sensors, meteorological variables were calculated in the 15 previous days of each epidemic observation. Using variables which integrate the effects of air temperature and humidity and frequency of light precipitations over the epidemic progress, logistic models were adjusted for estimating the probability of occurrrence of severe, moderate and nil epidemic increment rates, reaching maximum prediction accuracy of 92.5% (two variable models, N=40). Predicted values by the univariate model (selected by Stepwise procedure; prediction accuracy: 87.5%) were validated with the epidemic curve observed during 2000/2001 growing season. These model predictions could complement epidemic observations, giving regional scale to warning systems for improving the decision making process regarding disease chemical control.Fil: Oriolani, Enrique J. A.. Instituto Nacional de Tecnología Agropecuaria (Argentina). Estación Experimental Agropecuaria Mendoza.Fil: Moschini, Ricardo C.. Instituto de Clima y Agua. CIRN. INTA Castelar.Fil: Salas, Sergio. Instituto Nacional de Tecnología Agropecuaria (Argentina). Estación Experimental Agropecuaria Mendoza.Fil: Martinez, Malvina I.. Instituto de Clima y Agua. CIRN. INTA Castelar.Fil: Banchero, Santiago. Instituto de Clima y Agua. CIRN. INTA Castelar

Serendipitous discovery of RR Lyrae stars in the Leo V ultra-faint galaxy

During the analysis of RR Lyrae stars discovered in the High cadence Transient Survey (HiTS) taken with the Dark Energy Camera at the 4-m telescope at Cerro Tololo Inter-American Observatory, we found a group of three very distant, fundamental mode pulsator RR Lyrae (type ab). The location of these stars agrees with them belonging to the Leo V ultra-faint satellite galaxy, for which no variable stars have been reported to date. The heliocentric distance derived for Leo V based on these stars is 173 +/- 5 kpc. The pulsational properties (amplitudes and periods) of these stars locate them within the locus of the Oosterhoff II group, similar to most other ultra-faint galaxies with known RR Lyrae stars. This serendipitous discovery shows that distant RR Lyrae stars may be used to search for unknown faint stellar systems in the outskirts of the Milky Way.Comment: Accepted in ApJ Letter

Genotoxic evidences of glyphosate and chlorpyriphos on eisenia fetida coelomocytes

The organophosphorus herbicide glyphosate (GLY) and the organophosphate insecticide chlorpyriphos (CPF) are key pesticides in modern management cultures worldwide. Sublethal toxicity of the commercial herbicide formulation Roundup® and the insecticide formulation Terfos® were evaluated on Eisenia fetida coelomocytes exposed under in vivo and ex vivo laboratory conditions. Induction of DNA single-strand breaks evaluated by the single cell gel electrophoresis assay and coelomocyte viability as well as alterations in coelomocyte trophic indexes were employed as endpoints for genotoxicity and cytotoxicity, respectively. Specimens were exposed at concentrations corresponding to recommended pesticide field application rate, and endpoints were evaluated after 7 and 14 days of treatment (in vivo exposure). In addition, coelomocytes were exposed to aqueous leachate of pesticide-contaminated soils during 1 h (ex vivo exposure). Earthworms exposed to Roundup® and Terfos® showed an increased frequency of DNA damage. Also, a decrease of coelomocyte viability and decrease of trophic indexes were observed in all treatments. The results demonstrate that either GLY- and CPF-based formulations exerted genotoxic as well as cytotoxic effects in coelomocytes of E. fetida exposed in vivo and ex vivo.Fil: Curieses, Silvana Patricia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sáenz, María Elena. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alberdi, José Luis. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; ArgentinaFil: Martinez, Ricardo Santiago. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Di Marzio, Walter Dario. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Anômala contribuição bilateral do extensor longo do polegar e fusão dos músculos do primeiro compartimento dorsal do punho

Knowledge of the anatomical variations of the muscles of the first dorsal compartments of the wrist is clinically relevant to De Quervain's tenosynovitis and to reconstructive surgeries. In the literature, there are many reports of the presence of multiple insertion tendons in the first dorsal compartment of the wrist, but few reports describe occurrences of fusion and muscle contributions. This case report describes an anomalous bilateral contribution of the extensor pollicis longus. This anomalous contribution was found through a slender auxiliary tendon that crossed laterally under the extensor retinaculum, entered the first dorsal compartment of the wrist and merged with the tendon of the extensor pollicis brevis muscle. In the same cadaver in which this contribution was present, there was atypical muscle fusion of the abductor pollicis longus muscle and extensor pollicis brevis muscle. In conclusion, anomalous bilateral contribution of the extensor pollicis longus muscle and atypical muscle fusion, concomitant with a variant insertion pattern, are the highlight of this case report. Furthermore, it is concluded that additional tendons may be effectively used in reconstructive surgeries, but that there is a need for knowledge of the possible numerical and positional variations of these tendons, with a view to making more effective surgical plans.O conhecimento das variações anatômicas dos músculos do primeiro compartimento dorsal do punho é clinicamente relevante na tenossinovite de De Quervain e nas cirurgias reconstrutivas Na literatura encontram-se inúmeros relatos da presença de múltiplos tendões de inserção no primeiro compartimento dorsal do punho, mas são poucos os relatos que descrevem a ocorrência de fusão e de contribuições musculares. Este relato de caso descreve uma contribuição anômala bilateral do extensor longo do polegar. A anômala contribuição foi encontrada por um tendão auxiliar delgado que cruzou lateralmente sob o retináculo dos extensores, entrou no primeiro compartimento dorsal do punho e fundiu-se com o tendão do músculo extensor curto do polegar. No mesmo cadáver foi encontrada a presença dessa contribuição e uma fusão muscular atípica do músculo abdutor longo do polegar (APL) com o extensor curto do polegar (EPB). Em conclusão, a anômala contribuição bilateral do músculo extensor longo do polegar e a fusão muscular atípica, concomitante com um padrão de inserção variante, é o destaque deste relato de caso. Ainda, conclui que os tendões adicionais podem ser efetivamente usados nas cirurgias reconstrutivas e alerta sobre a necessidade de conhecimento das possíveis variações numéricas e posicionais desses tendões, com vistas a planejamentos cirúrgicos mais eficazes

Predicción de epidemias del oídio de la vid (Uncinula necator (Schwein) Burrill) mediante modelos basados en factores meteorológicos

Powdery mildew, caused by Uncinula necator (Schwein) Burrill is one of the most damaging disease affecting grapevines (Vitis vinifera L.) in Mendoza and worldwide, reducing yield and fruit quality. Throughout six growing seasons (2001/02; 2002/03; 2005/06; 2007/08; 2008/09; 2009/10), powdery mildew incidence values (expressed as daily epidemic increment rates) were observed in clusters from flowering to onset of ripening (change of color of the grape berries), in a grapevine planted with the susceptible Chenin cultivar at the EEA INTA Mendoza (Luján de Cuyo). From hourly values of thermal-moisture elements recorded by upper canopy sensors, meteorological variables were calculated in the 15 previous days of each epidemic observation. Using variables which integrate the effects of air temperature and humidity and frequency of light precipitations over the epidemic progress, logistic models were adjusted for estimating the probability of occurrrence of severe, moderate and nil epidemic increment rates, reaching maximum prediction accuracy of 92.5% (two variable models, N=40). Predicted values by the univariate model (selected by Stepwise procedure; prediction accuracy: 87.5%) were validated with the epidemic curve observed during 2000/2001 growing season. These model predictions could complement epidemic observations, giving regional scale to warning systems for improving the decision making process regarding disease chemical control.El oídio de la vid (Vitis vinifera L.), causado por Uncinula necator (Schwein) Burrill, es una enfermedad fúngica relevante en viñedos de la provincia de Mendoza, ocasionando pérdidas de producción y degradación de la calidad del fruto cosechado. Durante seis campañas agrícolas (2001/02; 2002/03; 2005/06; 2007/08; 2008/09; 2009/10), en un parral del cultivar susceptible Chenín (EEA INTA Mendoza, Luján de Cuyo), se observó la evolución de la incidencia del oídio en racimos (como tasa de incremento epidémica diaria), desde floración hasta envero. A partir de registros térmico-hídricos horarios (monitoreados con sensores ubicados en la parte superior del canopeo) se calcularon variables meteorológicas en los 15 días previos a cada observación del progreso de la epidemia. Con variables que integran efectos de la temperatura, humedad del aire y frecuencia de precipitaciones ligeras sobre el incremento de la enfermedad en función del tiempo, se ajustaron modelos de regresión logística para estimar las probabilidades de ocurrencia de tasas epidémicas categorizadas (severa, moderada y nula), logrando una precisión de predicción máxima de 92,5% (modelo bivariado, N=40). Las predicciones del modelo univariado (seleccionado por "Stepwise"; precisión de predicción: 87,5%) se validaron satisfactoriamente con la curva epidémica observada en 2000/2001. Los valores estimados por estos modelos podrían complementar a las observaciones epidémicas y emitir alarmas regionales que sustenten la toma de decisión de control químico

Los siete monstruos mitologicos

Anais do I Encontro de Iniciação Científica e de Extensão da Unila – Extensão: Grupo temático “Águas da Integração” - 05/06/12 - 08h00 às 18h00 - Unila-Centro - Salas 10 e 11 - 2o PisoEn el marco del programa aguas de la integración, se encuentra la investigación sobre el universo cosmológico de los guaraníes, esta es una búsqueda sobre las manifestaciones artísticas y culturales de los guaraníes y de como ellos conciben el universo, la vida, la naturaleza, la comunidad y la familia, la belleza de las cosas que nos rodean. Cuales son las formas en las ellos la interpretan, su manera de pensar y de como observan la vida. Una de las formas particulares de observar la vida y que llamo la atención de nuestra investigación son los mitos y leyendas, que han sido pasados de generación en generación de forma verbal, poseyendo la capacidad de enseñar y dar pensamientos de erudición para la vida cotidiana. Así como el cuidado de la naturaleza, la comunidad y de la familia, tratando hasta en muchas ocasiones de explicar lo que existe dentro de la naturaleza, como también las relaciones interpersonales y muchas otras cosas mas. Sus manifestaciones culturales como la danza, el contar mitos y leyendas, sus artesanías, lengua, costumbres entre otras tantas cosas, nosotros presentamos también la realidad como al mismo tiempo como ellos los guaraníes perciben la suya. Pero sobre todo enfatizamos sobre los mitos y las leyendas, de como estas hasta el día de hoy influyen en la cultura de esta región del Paraná. Para nosotros demostrar de cuanta importancia tiene esto para nuestra cultura y de tratar de no olvidar nuestras raíces y de mostrar a todos sobre la base en las cuales se fomenta la cultura paraguaya y también de la región del Paraná en la cual habitamos actualmente, el cual posee una innumerable riqueza cultural y que posee características que otras regiones no poseen. Hablaremos de forma continua sobre los mitos y leyendas, de sus principales actores y como estos singulares personajes en sus actividades y acciones, tienen algo que mostrarnos y enseñarnos a medida que transcurran las presentaciones de los mismos. Es importante destacar que muchos de estos mitos y leyendas están en guaraní y que para poder hacerlos comprender tuvimos que realizar adaptaciones y traducciones al portugués, para la mejor compresión y discernimiento de todo lo presentado.Universidade Federal da Integração Latino-Americana (UNILA

Heart transplantation in amyloidosis: Clinical and imaging manifestations

Objetivo: Describir las características clínicas e imagenológicas de los pacientes con trasplante cardiaco por amiloidosis en una institución de la comunidad. Método: Serie de casos descriptiva de pacientes consecutivos receptores de trasplante cardiaco con amiloidosis en un centro médico. Se incluyeron todos los pacientes con diagnóstico de amiloidosis con compromiso cardiaco receptores de trasplante cardiaco en el periodo de noviembre de 2008a febrero de 2021. Resultados: Se incluyeron 16 pacientes con una edad media de 59.9 años (± 10.2) y el 81.25 % (n = 13) eran de sexo masculino. Según el tipo de amiloidosis, 12 pacientes recibieron el trasplante por amiloidosis por cadenas livianas (AL) y 4 por amiloidosis por transtiretina (ATTR). Las formas más frecuentes de presentación clínica fueron sobrecarga izquierda (50 %) y shock cardiogénico (32 %). La mitad recibieron el trasplante estando en lista de emergencia. La fracción de eyección promedio previa al trasplante fue del 43% (± 16). Presentaron disfunción del ventrículo derecho 14 de los 16 pacientes. El hallazgo más común en la resonancia magnética cardiaca fue el patrón de realce tardío de gadolinio subendocárdico difuso, con anulación del pool sanguíneo. La realización del trasplante cardiaco permitió a la mitad de los pacientes con amiloidosis AL (n = 6) la posibilidad de recibir trasplante de médula ósea en un segundo tiempo. Conclusiones: En la actualidad, el trasplante cardiaco se ha convertido en una opción para pacientes con insuficiencia cardiaca por amiloidosis, tanto AL como ATTR. En los pacientes con amiloidosis AL incluso puede permitir en un segundo tiempo el trasplante de médula ósea.Objective: To describe the clinical and imaging characteristics of heart transplantation patients due to amyloidosis in a community institution. Method: Descriptive case series of consecutive heart transplantation patients with amyloidosis in a medical center. All patients with diagnosis of amyloidosis with cardiac compromise receiving heart transplantation, performed in the period November 2008 to February 2021, were included in the analysis. Results: A total of 16 patients were included. The mean age was 59.9 years (± 10.2). 81.25% (n = 13) were male. According to the type of amyloidosis, 12 patients were transplanted for AL amyloidosis and 4 for ATTR amyloidosis. The most frequent clinical presentations were left overload (50%) and cardiogenic shock (32%). The mean ejection fraction prior to transplantation was 43% (± 16), 14 of the 16 patients had right ventricular dysfunction. The most common finding on cardiac magnetic resonance was the diffuse subendocardial late gadolinium enhancement pattern, with cancellation of the blood pool. The heart transplantation gave 6 patients the chance to receive a bone marrow transplantation afterwards. Conclusions: Heart transplantation has become an option for patients with heart failure due to AL and ATTR amyloidosis. In patients with AL amyloidosis, it might even allow bone marrow transplantation in a second stage.Fil: Decotto, Santiago. Hospital Italiano; ArgentinaFil: Villanueva, Eugenia. Hospital Italiano; ArgentinaFil: Pérez de Arenaza, Diego. Hospital Italiano; ArgentinaFil: Nucifora, Elsa Mercedes. Hospital Italiano; ArgentinaFil: Aguirre, Maria Adela. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Posadas Martinez, Maria Lourdes. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marenchino, Ricardo G.. Hospital Italiano; ArgentinaFil: Belziti, César. Hospital Italiano; Argentin

Associations among MHC genes, latitude, and avian malaria infections in the rufous-collared sparrow (Zonotrichia capensis).

The major histocompatibility complex (MHC) is a genetic region in jawed vertebrates that contains key genes involved in the immune response. Associations between the MHC and avian malaria infections in wild birds have been observed and mainly explored in the Northern Hemisphere, while a general lack of information remains in the Southern Hemisphere. Here, we investigated the associations between the MHC genes and infections with Plasmodium and Haemoproteus blood parasites along a latitudinal gradient in South America. We sampled 93 rufous-collared sparrows (Zonotrichia capensis) individuals from four countries, Colombia, Ecuador, Peru, and Chile, and estimated MHC-I and MHC-II allele diversity. We detected between 1-4 (MHC-I) and 1-6 (MHC-II) amino acidic alleles per individual, with signs of positive selection. We obtained generalized additive mixed models to explore the associations between MHC-I and MHC-II diversity and latitude. We also explored the relationship between infection status and latitude/biome. We found a non-linear association between the MHC-II amino acidic allele diversity and latitude. Individuals from north Chile presented a lower MHC genetic diversity than those from other locations. We also found an association between deserts and xeric shrublands and a lower prevalence of Haemoproteus parasites. Our results support a lower MHC genetic in arid or semi-arid habitats in the region with the lower prevalence of Haemoproteus parasites

MYH10 activation rescues contractile defects in arrhythmogenic cardiomyopathy (ACM).

The most prevalent genetic form of inherited arrhythmogenic cardiomyopathy (ACM) is caused by mutations in desmosomal plakophilin-2 (PKP2). By studying pathogenic deletion mutations in the desmosomal protein PKP2, here we identify a general mechanism by which PKP2 delocalization restricts actomyosin network organization and cardiac sarcomeric contraction in this untreatable disease. Computational modeling of PKP2 variants reveals that the carboxy-terminal (CT) domain is required for N-terminal domain stabilization, which determines PKP2 cortical localization and function. In mutant PKP2 cells the expression of the interacting protein MYH10 rescues actomyosin disorganization. Conversely, dominant-negative MYH10 mutant expression mimics the pathogenic CT-deletion PKP2 mutant causing actin network abnormalities and right ventricle systolic dysfunction. A chemical activator of non-muscle myosins, 4-hydroxyacetophenone (4-HAP), also restores normal contractility. Our findings demonstrate that activation of MYH10 corrects the deleterious effect of PKP2 mutant over systolic cardiac contraction, with potential implications for ACM therapy.This study was supported by MCIU grant BFU2016-75144-R and PID2020- 116935RB-I00, and by a “la Caixa” Banking Foundation grant under the project code HR18-00304” to J.A.B.; The study was also supported by the “Ayudas a la Investigación Cátedra Real Madrid-Universidad Europea” (2017/RM01). C.M.-L. and S.S. hold MCIU predoctoral contracts BES-2017-079715, and BES-2017-079707 respectively. R.G. acknowledges funding from the European Research Council under grant ERCAG-340177 (3DNanoMech) and from the MCIU under grant MAT2016- 76507-R. The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación (MCIN) and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence, grant CEX2020-001041-S funded by MICIN/AEI/10.13039/501100011033. The microscopy experiments were carried out at the Dynamic Microscopy and Image Unit, CNIC, ICTS-ReDib, co-financed by MCIN/AEI /10.13039/ 501100011033 and FEDER “A way of making Europe” (#ICTS-2018-04- CNIC-16). Imaris full analysis were carried out at the Microscopy & Dynamic Imaging, CNIC, ICTS-ReDib, co-funded by MCIN/AEI /10.13039/501100011033. Biomedical Imaging has been conducted at the Advanced Imaging Unit of the CNIC (Centro Nacional de Investigaciones Cardiovasculares Carlos III), Madrid, Spain. This project used the ReDIB ICTS infrastructure TRIMA@CNIC, Ministerio de Ciencia e Innovación (MCIN).S

Insulin regulates neurovascular coupling through astrocytes

Mice with insulin receptor (IR)-deficient astrocytes (GFAP-IR knockout [KO] mice) show blunted responses to insulin and reduced brain glucose uptake, whereas IRdeficient astrocytes show disturbed mitochondrial responses to glucose. While exploring the functional impact of disturbed mitochondrial function in astrocytes, we observed that GFAP-IR KO mice show uncoupling of brain blood flow with glucose uptake. Since IR-deficient astrocytes show higher levels of reactive oxidant species (ROS), this leads to stimulation of hypoxia-inducible factor-1¿ and, consequently, of the vascular endothelial growth factor angiogenic pathway. Indeed, GFAP-IR KO mice show disturbed brain vascularity and blood flow that is normalized by treatment with the antioxidant N-acetylcysteine (NAC). NAC ameliorated high ROS levels, normalized angiogenic signaling and mitochondrial function in IR-deficient astrocytes, and normalized neurovascular coupling in GFAP-IR KO mice. Our results indicate that by modulating glucose uptake and angiogenesis, insulin receptors in astrocytes participate in neurovascular coupling.We are thankful to M.Garcia and R. Cañadas for technical support. This work was funded by Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED) (Instituto de Salud CarlosIII, Spain) to I.T.A., A.G., and T.I.; an Inter-CIBER project (PIE14/00061) to I.T.A.that forms part of the projects PID2019-104376RB-I00 (I.T.A.) and RTI2018-094887-B-I00 (M.N.) funded by MCIN/AEI/10.13039/501100011033; a grant from Junta de Andalucia Consejería de Economía y Conocimiento (P18-RT-2233 to A.G.) cofinanced by Programa Operativo FEDER 2014–2020; a grant from Instituto de Salud Carlos III Spain (cofinanced by FEDER funds from the European Union; PI21/00915 to A.G.); Grant PID2020-115218RB-I00 to T.I. funded by Ministerio de Ciencia e Innovación/Agencia Española de Investigación (MCIN/AEI/10.13039/501100011033); and a grant from Comunidad de Madrid through the European Social Fund (ESF)–financed programme Neurometabolismo-Comunidad de Madrid (NEUROMETAB-CM) (B2017/BMD-3700 to I.T.A.and T.I.). M.N. was also supported by the Spanish Ministry of Science and Innovation (Ramón y Cajal RYC-2016-20414). J.P.-U. was contracted by CIBERNED