Short-term effects of impurities in the CO2 stream injected into fractured carbonates.

After the Paris Agreement in which 195 countries are involved, the Carbon Capture and Storage (CCS) is now an accepted technology in the United Nations Framework Convention on Climate Change (UNFCCC). In Spain, Fundación Ciudad de la Energía (CIUDEN) has successfully completed the full CCS chain, being CO2 captured in the Technology Development Centre in Cubillos del Sil (León, Spain) whereas that it is geologically stored in a deep saline aquifer, formed by fractured carbonates with poor matrix porosity, located in the Technology Development Plant (TDP) at Hontomín (Burgos, Spain). The results of the field tests, in which up to 150 tons of CO2 and synthetic air (5 %v of N2 and O2) were co-injected on site, are analyzed in this paper comparing the operational parameters gained during the injection of impure CO2 (pressures, temperatures and flow ranges) with its corresponding baseline previously determined (i.e. 1,500 tons of pure CO2 were injected during the reservoir hydraulic characterization). Besides that, the geochemical reactivity analysis of impure CO2 injected in this saline aquifer and its correlation with the results from laboratory tests were assessed. As main conclusions from laboratory scale results, a porosity diminution was measured after the injection of CO2 with 5 %v of SO2; apart from that, without SO2, the effluent pH was in the range of 7-8 whereas in case of CO2 and SO2, a pH of 1-2 was obtained. Otherwise and focused on field scale tests, a density decrease was detected comparing the base case (pure CO2) with the CO2 injection containing 5.1 %v of synthetic air. On the other hand, Ca2+, SO42-, Mg2+ and K+ migration effects in the rock were also detected and analyzed

Group 10 Metal Benzene-1,2-dithiolate Derivatives in the Synthesis of Coordination Polymers Containing Potassium Countercations

This document is the Accepted Manuscript version of a Published Work that appeared in final form in Inorganic Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/abs/10.1021/acs.inorgchem.7b01775The use of theoretical calculations has allowed us to predict the coordination behavior of dithiolene [M(SC6H4S)2]2- (M = Ni, Pd, Pt) entities, giving rise to the first organometallic polymers {[K2(μ-H2O)2][Ni(SC6H4S)2]}n and {[K2(μ-H2O)2(thf)]2[K2(μ-H2O)2(thf)2][Pd3(SC6H4S)6]}n by one-pot reactions of the corresponding d10 metal salts, 1,2-benzenedithiolene, and KOH. The polymers are based on σ,π interactions between potassium atoms and [M(SC6H4S)2]2- (M = Ni, Pd) entities. In contrast, only σ interactions are observed when the analogous platinum derivative is used instead, yielding the coordination polymer {[K2(μ-thf)2][Pt(SC6H4S)2]}nThis work was supported in part by the MICINN (grants MAT2016-77608-C3-1-P and MAT2016-75883-C2-1-P), Generalitat Valenciana (PrometeoII/2014/076), and ISIC. J.I.M. acknowledges financial support by the “Ramon y Cajal ́ ” Program of MINECO (RYC-2015-17730

Estudio del desarrollo corporal de bovinos cruzados Wagyu-Brahman en el trópico húmedo, Panamá: Study of body development of Wagyu-Brahman crossbred cattle in the humid tropics, Panama

El objetivo principal fue estudiar el desarrollo corporal de bovinos cruzados Wagyu-Brahman bajo condiciones del trópico húmedo. Los datos provienen de la EEG-CMO-IDIAP (2008-2014). Las variables dependientes fueron: peso al nacimiento (PN), peso vivo (PVKG), cambio de peso vivo (CPV), tasa relativa de crecimiento (TRC), largo corporal (LC), altura a la cadera (AC) y perímetro torácico (PT). Los grupos raciales del padre (GRP) fueron: BRH y WG. Los grupos raciales de la madre (GRM) fueron: BRH, CRUCE, WG50 y WGF1. Los grupos raciales de la progenie (GRANI) fueron; WG25, WG50, WG75 y WGF1. Los datos se analizaron mediante un modelo lineal mixto donde año (YR) y época dentro del año [EP(YR)] fueron efectos aleatorios. El GRP y GRM(GRP) fueron efectos fijos. Además, PVKG, LC, AC y PT de cada GRANI fueron regresados con un modelo no lineal de Brody con la edad. Progenies de BRH tuvieron mayor PN (7,6 %), CPV (20,4 %) y TRC (10,8 %). El GRP como WG, y GRM como WGF1, BRH y CRUCE tuvieron mayor PN (31,5 kg), CPV (0,535 kg/d) y TRC (0,42 %), respectivamente. El modelo de Brody ajustó bien (P&lt;0,01) a los datos de cada GRANI. Se concluye que las variables del desarrollo corporal de los animales fueron afectadas por GRP y GRM(GRP) y que los efectos aleatorios de YR y EP(YR) permitieron reducir sesgos. El modelo no lineal de Brody ajustó bien a los datos de las variables de desarrollo corporal.El objetivo principal fue estudiar el desarrollo corporal de bovinos cruzados Wagyu-Brahman bajo condiciones del trópico húmedo. Los datos provienen de la EEG-CMO-IDIAP (2008-2014). Las variables dependientes fueron: peso al nacimiento (PN), peso vivo (PVKG), cambio de peso vivo (CPV), tasa relativa de crecimiento (TRC), largo corporal (LC), altura a la cadera (AC) y perímetro torácico (PT). Los grupos raciales del padre (GRP) fueron: BRH y WG. Los grupos raciales de la madre (GRM) fueron: BRH, CRUCE, WG50 y WGF1. Los grupos raciales de la progenie (GRANI) fueron; WG25, WG50, WG75 y WGF1. Los datos se analizaron mediante un modelo lineal mixto donde año (YR) y época dentro del año [EP(YR)] fueron efectos aleatorios. El GRP y GRM(GRP) fueron efectos fijos. Además, PVKG, LC, AC y PT de cada GRANI fueron regresados con un modelo no lineal de Brody con la edad. Progenies de BRH tuvieron mayor PN (7,6 %), CPV (20,4 %) y TRC (10,8 %). El GRP como WG, y GRM como WGF1, BRH y CRUCE tuvieron mayor PN (31,5 kg), CPV (0,535 kg/d) y TRC (0,42 %), respectivamente. El modelo de Brody ajustó bien (P<0,01) a los datos de cada GRANI. Se concluye que las variables del desarrollo corporal de los animales fueron afectadas por GRP y GRM(GRP) y que los efectos aleatorios de YR y EP(YR) permitieron reducir sesgos. El modelo no lineal de Brody ajustó bien a los datos de las variables de desarrollo corporal.The main objective was to study the body development of crossbred Wagyu-Brahman bovines under humid tropical conditions. Data came from EEG-CMO-IDIAP (2008-2024). Dependent variables were birth weight (PN), live weight (PVKG), live weight change (CPV), relative growth rate (TRC), body length (LC), hip height (AC), and thorax perimeter (PT). Sire breed group (GRP) were BRH and WG. Dam breed group (GRM) were BRH, CRUCE, WG50, and WGF1. Progeny breed group (GRANI) were WG25, WG50, WG75, and WGF1. Data were analyzed by a mixed linear model where year (YR) and season within year [EP(YR)] were randoms and GRP and GRM(GRP) were fixed. Besides, PVKG, LC, AC, and PT for each GRANI were regressed with a Brody non-linear model on age. Brahman progenies had higher PN (7,6 %), CPV (20,4 %) and TRC (10,8 %). The GRP like WG, and GRM like WGF1, BRH, and CRUCE had higher PN (31,5 kg), CPV (0,535 kg/d), and TRC (0,42 %), respectively. Brody non-linear model adjusted well (P<0,01) to data of each GRANI. It was concluded that body development variables of the animals were affected by GRP and GRM(GRP) and random effects of YR and EP(YR) allowed to reduce bias. The Brody non-linear model adjusted well to the body development data

The prognostic impact of minimal residual disease in patients with chronic lymphocytic leukemia requiring first-line therapy.

A proportion of patients with chronic lymphocytic leukemia achieve a minimal residual disease negative status after therapy. We retrospectively evaluated the impact of minimal residual disease on the outcome of 255 consecutive patients receiving any front-line therapy in the context of a detailed prognostic evaluation, including assessment of IGHV, TP53, NOTCH1 and SF3B1 mutations. The median follow-up was 73 months (range, 2-202) from disease evaluation. The median treatment-free survival durations for patients achieving a complete response without or with minimal residual disease, a partial response and no response were 76, 40, 11 and 11 months, respectively (P<0.001). Multivariate analysis revealed that three variables had a significant impact on treatment-free survival: minimal residual disease (P<0.001), IGHV status (P<0.001) and β2-microglobulin levels (P=0.012). With regards to overall survival, factors predictive of an unfavorable outcome were minimal residual disease positivity (P=0.014), together with advanced age (P<0.001), unmutated IGHV status (P=0.001), TP53 mutations (P<0.001) and elevated levels of β2-microglobulin (P=0.003). In conclusion, for patients requiring front-line therapy, achievement of minimal residual disease negativity is associated with significantly prolonged treatment-free and overall survival irrespective of other prognostic markers or treatment administered

Proteasome Dysfunction Associated to Oxidative Stress and Proteotoxicity in Adipocytes Compromises Insulin Sensitivity in Human Obesity

AIMS: Obesity is characterized by a low-grade systemic inflammatory state and adipose tissue (AT) dysfunction, which predispose individuals to the development of insulin resistance (IR) and metabolic disease. However, a subset of obese individuals, referred to as metabolically healthy obese (MHO) individuals, are protected from obesity-associated metabolic abnormalities. Here, we aim at identifying molecular factors and pathways in adipocytes that are responsible for the progression from the insulin-sensitive to the insulin-resistant, metabolically unhealthy obese (MUHO) phenotype. RESULTS: Proteomic analysis of paired samples of adipocytes from subcutaneous (SC) and omental (OM) human AT revealed that both types of cells are altered in the MUHO state. Specifically, the glutathione redox cycle and other antioxidant defense systems as well as the protein-folding machinery were dysregulated and endoplasmic reticulum stress was increased in adipocytes from IR subjects. Moreover, proteasome activity was also compromised in adipocytes of MUHO individuals, which was associated with enhanced accumulation of oxidized and ubiquitinated proteins in these cells. Proteasome activity was also impaired in adipocytes of diet-induced obese mice and in 3T3-L1 adipocytes exposed to palmitate. In line with these data, proteasome inhibition significantly impaired insulin signaling in 3T3-L1 adipocytes. INNOVATION: This study provides the first evidence of the occurrence of protein homeostasis deregulation in adipocytes in human obesity, which, together with oxidative damage, interferes with insulin signaling in these cells. CONCLUSION: Our results suggest that proteasomal dysfunction and impaired proteostasis in adipocytes, resulting from protein oxidation and/or misfolding, constitute major pathogenic mechanisms in the development of IR in obesity.IMIBIC/Universidad de Córdoba-SCAI (ProteoRed, PRB2-ISCIII)MINECO/FEDERJunta de Andalucía/FEDERCIBERobn(Instituto de Salud Carlos III

Composición arquitectónica: Historia, Teoría, Crítica y Patrimonio de la Arquitectura

La presente Memoria realiza balance de todas las asignaturas del área de conocimiento Composición Arquitectónica (acCA), una vez que se han implantado los cinco cursos del plan de Grado en Arquitectura (2010) y que este se está sustituyendo por el nuevo Grado en Fundamentos en Arquitectura (2014). Las asignaturas en ambos planes son prácticamente las mismas tanto en contenidos como en posición en los distintos cursos. Esta tarea se efectúa como chequeo de los objetivos trazados hace cinco años cuando se diseñó una nueva estructura docente y un nuevo método de aprendizaje (teórico y práctico) con el fin de adaptar la titulación de Arquitectura al nuevo EEES. De este modo, se repasan, para todas las asignaturas, los contenidos previstos en los descriptores de las Memorias de Grado, así como el modo de impartirlos, el sistema de prácticas, el proceso de evaluación y los resultados obtenidos. Se aprovecha estos años de experiencia para señalar las variaciones introducidas en las materias para ajustarlas a la realidad del tiempo disponible, del número de alumnos, de los avances tecnológicos y del presente social y cultural. Esta Memoria da cuenta de la satisfacción del profesorado y del alumnado, pero también señala sus limitaciones

Galaxy clusters and groups in the ALHAMBRA Survey

We present a catalogue of 348 galaxy clusters and groups with $0.2<z<1.2$ selected in the 2.78 $deg^2$ ALHAMBRA Survey. The high precision of our photometric redshifts, close to $1\%$, and the wide spread of the seven ALHAMBRA pointings ensure that this catalogue has better mass sensitivity and is less affected by cosmic variance than comparable samples. The detection has been carried out with the Bayesian Cluster Finder (BCF), whose performance has been checked in ALHAMBRA-like light-cone mock catalogues. Great care has been taken to ensure that the observable properties of the mocks photometry accurately correspond to those of real catalogues. From our simulations, we expect to detect galaxy clusters and groups with both $70\%$ completeness and purity down to dark matter halo masses of $M_h\sim3\times10^{13}\rm M_{\odot}$ for $z<0.85$. Cluster redshifts are expected to be recovered with $\sim0.6\%$ precision for $z<1$. We also expect to measure cluster masses with $\sigma_{M_h|M^*_{CL}}\sim0.25-0.35\, dex$ precision down to $\sim3\times10^{13}\rm M_{\odot}$, masses which are $50\%$ smaller than those reached by similar work. We have compared these detections with previous optical, spectroscopic and X-rays work, finding an excellent agreement with the rates reported from the simulations. We have also explored the overall properties of these detections such as the presence of a colour-magnitude relation, the evolution of the photometric blue fraction and the clustering of these sources in the different ALHAMBRA fields. Despite the small numbers, we observe tentative evidence that, for a fixed stellar mass, the environment is playing a crucial role at lower redshifts (z$<$0.5).Comment: Accepted for publication in MNRAS. Catalogues and figures available online and under the following link: http://bascaso.net46.net/ALHAMBRA_clusters.htm

High prevalence and mortality due to Histoplasma capsulatum in the Brazilian Amazon: An autopsy study

Background: Histoplasmosis is acquired by inhalation of spores of the dimorphic fungus Histoplasma spp. Although this pathogen is distributed worldwide, it is more prevalent in the Americas. However, the real burden of histoplasmosis remains undefined in many endemic regions. Methodology: We conducted a series of 61 autopsies to individuals who died in a hospital in the Brazilian Amazon focused on infectious diseases. We performed a detailed histological and microbiological evaluation with genetic characterization of Histoplasma strains with the aim to evaluate the contribution of histoplasmosis to morbidity and mortality. Additionally, we assessed the clinicopathological correlation. Principal findings: Evidence of Histoplasma infection was detected in 21 patients (34%). Eight cases were disseminated infections, all of them occurred in HIV-positive patients. Six cases were localized histoplasmosis, limited to the lungs. In seven patients Histoplasma DNA was detected by PCR in patients with no histological lesions. Histoplasma infection was detected in 38% of HIV-positive patients and was a major contributor to death in 22% of them. Lungs, liver and spleen were affected in all cases of disseminated histoplasmosis. Phylogenetic analysis of the strains suggested a high diversity of Histoplasma species circulating in the Brazilian Amazon. Histoplasmosis was clinically missed in 75% of the disseminated infections. Conclusions: The high incidence of histoplasmosis, the low index of clinical suspicion, and the severity of the disseminated disease highlight the need of proactively implementing sensitive routine screening methods for this pathogen in endemic areas. Antifungal prophylaxis against Histoplasma should be encouraged in the severely immunocompromised HIV patients in these areas. In conclusion, substantial mortality is associated with disseminated histoplasmosis among HIV-positive patients in the Brazilian Amazon

Structure-based statistical analysis of transmembrane helices

Recent advances in determination of the high-resolution structure of membrane proteins now enable analysis of the main features of amino acids in transmembrane (TM) segments in comparison with amino acids in water-soluble helices. In this work, we conducted a large-scale analysis of the prevalent locations of amino acids by using a data set of 170 structures of integral membrane proteins obtained from the MPtopo database and 930 structures of water-soluble helical proteins obtained from the protein data bank. Large hydrophobic amino acids (Leu, Val, Ile, and Phe) plus Gly were clearly prevalent in TM helices whereas polar amino acids (Glu, Lys, Asp, Arg, and Gln) were less frequent in this type of helix. The distribution of amino acids along TM helices was also examined. As expected, hydrophobic and slightly polar amino acids are commonly found in the hydrophobic core of the membrane whereas aromatic (Trp and Tyr), Pro, and the hydrophilic amino acids (Asn, His, and Gln) occur more frequently in the interface regions. Charged amino acids are also statistically prevalent outside the hydrophobic core of the membrane, and whereas acidic amino acids are frequently found at both cytoplasmic and extra-cytoplasmic interfaces, basic amino acids cluster at the cytoplasmic interface. These results strongly support the experimentally demonstrated biased distribution of positively charged amino acids (that is, the so-called the positive-inside rule) with structural data

Factors associated with the clinical outcome of patients with relapsed/refractory CD19+acute lymphoblastic leukemia treated with ARI-0001 CART19-cell therapy

The prognosis of patients with relapsed/refractory (R/R) acute lymphoblastic leukemia (ALL) remains poor, particularly for those relapsing after allogeneic hema-topoietic cell transplantation (alloHCT). Novel agents such as inotuzumab ozogamicin or blinatumomab achieve increased response rates, but these are generally transient unless followed by alloHCT. Chimeric antigen receptors (CAR) targeting CD19 have shown promising results in R/R ALL, and one of these products (tisagenlecleucel) has been approved for the treatment of patients with R/R ALL up to 25 years of age