Assessment of the specificity of cardiopulmonary response during tethered swimming using a new snorkel device

This study aimed at comparing maximal oxygen uptake (VO2max), maximal heart rate (HRmax), and anaerobic threshold (AT) obtained from tethered swimming (SW) and three other testing procedures: cycling (CY), running (RU), and arm cranking (AC). Variables were assessed in 12 trained male swimmers by a portable gas analyzer connected to a modified snorkel system to allow expired gases collection during swimming. Athletes exhibited a higher VO2max during the SW test as compared to the CY and the AC tests. There was no significant difference in VO2max between the SW and the RU test, but the Bland and Altman plot highlighted a poor agreement between results. Moreover, AT occurred at higher workloads during SW in comparison to the other tests. These results do not support the use of any unspecific testing procedures to estimate VO2max, HRmax, and AT for swimming

Efficacy of Relaxin on functional recovery of post stroke patients

Background. Relaxin is a peptide hormone that exerts specific effects on cardiovascular system and human brain, leading to the hypothesis that this hormone may play a protective role against CVD and integration and modulation of behavioral activation. We aimed to demonstrate the efficacy of Relaxin on functional recovery of post-stroke patients. Methods. Patients admitted within a Rehabilitation Unit suffering from stroke have been evaluated. Patients have been randomized to RLX (40 mcg/d) plus rehabilitation vs a control group that underwent only rehabilitation. A preliminary analysis of 36 patients at 20 and 40 days was made using the mRS for global function, the Functional Independent Measure (FIM) for daily activity and Trail Making Test (TMT) for cognitive function. Results. Eighteen patients (age 72 (64-79), M 56%) randomized to RLX plus rehabilitation were compared to 18 patients (age 68 (64-78), M 50%) that underwent only rehabilitation. There was no difference between the two groups in terms of risk factors, stroke syndromes and etiology. At admission the two groups showed the same characteristics in terms of functional aspects (mRS, FIM; p ns) and cognitive function (TMT; p ns). After 20 days (T1) the treatment group (RLX+rehabilitation) showed no differences between the two groups (FIM 78 vs 69; p ns), while after 40 days (T2) patients treated with RLX+R showed an excellent recovery (FIM 96 vs 75; p0.001). In terms of cognitive function patients RLX+R revealed a better performance at T1 ( TMT 3.5 vs 2; p 0.002) and still better at T2 (TMT 4 vs 2; p 0.001). These results have been confirmed in terms of global function both at T1 (mRS 2.5 vs 3; p0.001) and T2 (mRS 2 vs 3; p <0.001) . Conclusion. Relaxin showed in this analysis a positive effects on stroke patient’s recovery, thus offering the broad therapeutic potential role of RLX as new drug in post-stroke patients

Efficacy and safety of oral porcine relaxin (pRLX) in adjunct to physical exercise in the treatment of peripheral arterial disease (PAD)

Introduction PAD medical therapy has a number of limitations. RLX showed promises in experimental model mainly through NO release. Our study is the first to evaluate the efficacy and safety of RLX in PAD. Materials-Methods eligible PAD La fontaine IIa-IIb patients were randomized in 2 groups. Group A was treated with physical therapy plus oral pRLX, 20 ug b.i.d for 12 weeks, group B received physical therapy alone. Pain Free Walking Distance (PFWD) and Maximum Walking Distance (MWD) at 3 and 12 wks and at follow up 3 months after treatment interruption were performed. Results The percentage increases of PFWD in group B were 23±9, 65±17, and 35±4 respectively at 3 and at 12 weeks. and 3 months after termination. In Group A showed significantly higher percentage increases: 74±16 p<0.01, 168±28 p<0.001, and 122±15 p<0.001 at the corresponding time points. The percentage increases of MWD in the B group were 29±7, 55±10 and 54±8 at the above time points, while in the A group were 55±10 p<0.001, and 99±12 p<0.001. The RLX patients referred a better physical and mental status. No adverse events during or after the treatment were recorded. Comment-RLX resulted very effective in PAD. Our results may suggest that the observed functional benefits should come not only from hemodynamic improvement but also from positive vascular remodeling

Genetic loci linked to Type 1 Diabetes and Multiple Sclerosis families in Sardinia

<p>Abstract</p> <p>Background</p> <p>The Mediterranean island of Sardinia has a strikingly high incidence of the autoimmune disorders Type 1 Diabetes (T1D) and Multiple Sclerosis (MS). Furthermore, the two diseases tend to be co-inherited in the same individuals and in the same families. These observations suggest that some unknown autoimmunity variant with relevant effect size could be fairly common in this founder population and could be detected using linkage analysis.</p> <p>Methods</p> <p>To search for T1D and MS loci as well as any that predispose to both diseases, we performed a whole genome linkage scan, sequentially genotyping 593 microsatellite marker loci in 954 individuals distributed in 175 Sardinian families. In total, 413 patients were studied; 285 with T1D, 116 with MS and 12 with both disorders. Model-free linkage analysis was performed on the genotyped samples using the Kong and Cox logarithm of odds (LOD) score statistic.</p> <p>Results</p> <p>In T1D, aside from the HLA locus, we found four regions showing a lod-score ≥1; 1p31.1, 6q26, 10q21.2 and 22q11.22. In MS we found three regions showing a lod-score ≥1; 1q42.2, 18p11.21 and 20p12.3. In the combined T1D-MS scan for shared autoimmunity loci, four regions showed a LOD >1, including 6q26, 10q21.2, 20p12.3 and 22q11.22. When we typed more markers in these intervals we obtained suggestive evidence of linkage in the T1D scan at 10q21.2 (LOD = 2.1), in the MS scan at 1q42.2 (LOD = 2.5) and at 18p11.22 (LOD = 2.6). When all T1D and MS families were analysed jointly we obtained suggestive evidence in two regions: at 10q21.1 (LOD score = 2.3) and at 20p12.3 (LOD score = 2.5).</p> <p>Conclusion</p> <p>This suggestive evidence of linkage with T1D, MS and both diseases indicates critical chromosome intervals to be followed up in downstream association studies.</p

Genome-wide Analyses Identify KIF5A as a Novel ALS Gene

To identify novel genes associated with ALS, we undertook two lines of investigation. We carried out a genome-wide association study comparing 20,806 ALS cases and 59,804 controls. Independently, we performed a rare variant burden analysis comparing 1,138 index familial ALS cases and 19,494 controls. Through both approaches, we identified kinesin family member 5A (KIF5A) as a novel gene associated with ALS. Interestingly, mutations predominantly in the N-terminal motor domain of KIF5A are causative for two neurodegenerative diseases: hereditary spastic paraplegia (SPG10) and Charcot-Marie-Tooth type 2 (CMT2). In contrast, ALS-associated mutations are primarily located at the C-terminal cargo-binding tail domain and patients harboring loss-of-function mutations displayed an extended survival relative to typical ALS cases. Taken together, these results broaden the phenotype spectrum resulting from mutations in KIF5A and strengthen the role of cytoskeletal defects in the pathogenesis of ALS.Peer reviewe

Oltre il Segno/OltreMare

La realizzazione di un volume contenente le incisioni scelte all’interno della Scuola di Grafica d’Arte dell’Accademia di Belle Arti di Palermo, coordinata dai Proff. Giovanni D’Alessandro e Riccardo Mazzarino rappresenta motivo di orgoglio e di soddisfazione per la nostra Istituzione che costruisce i percorsi didattici dei propri corsi a partire dall’esperienza laboratoriale. L’incisione grafica è tra le tecniche artistiche più antiche ma nel contempo più contemporanee. La gestualità intrinseca al segno, che si manifesta nella carta, svela universi della visione inaspettati.(Mario Zito - Direttore dell’Accademia di Belle Arti di Palermo) Il segno è il risultato di un gesto a volte deciso, a volte contorto, a volte leggero, i cui risultati spesso sono inattesi e sorprendenti. Il volume contiene esemplari di incisioni fortemente caratterizzanti della scuola di Grafica d’Arte che vanta all’interno del proprio corso di studi docenti-artisti che consapevoli della ricchezza del loro bagaglio esperienziale offrono agli studenti gli strumenti necessari per far sì che l’arte del saper fare artigianale, si trasformi in mera poetica artistica

Visibilità delle figure femminili dell'universo mafioso: un'indagine attraverso la rassegna stampa nell'arco di un ventennio

Un utile strumento di analisi attraverso cui esplorare l'evoluzione nel tempo del ruolo delle figure femminili nella criminalità organizzata, è quello che si avvale della rassegna stampa tematica, raccolta nel periodo compreso tra il 1980 e il 2001. La scelta del periodo da prendere in esame - strettamente connessa agli orientamenti più generali del progetto di ricerca e, comunque, vincolata dalla necessità di delimitare l'ambito di reperimento e raccolta del materiale - è stata guidata dalla considerazione che proprio il ventennio compreso tra gli anni '80 e i nostri giorni - come emerge dalle indagini e dalle riflessioni finora prodotte in questo settore - sia da ritenere il più interessante per lo studio del fenomeno, in quanto proprio in questa fase la partecipazione delle donne alle attività criminali di stampo mafioso sembra divenuta più visibile e frequente

Multidisciplinary tephrochronological correlation of marker events in the eastern Tyrrhenian Sea between 48 and 105ka

The basal portion (2.92–5.08 m from core top) of the CET1 core located in a bathyal area of the eastern Tyrrhenian Sea is the subject of a multidisciplinary investigation, encompassing tephrostratigraphy, quantitative analyses of planktonic foraminifera and calcareous nannofossil assemblages, and δ18O measurements, supported by a 40Ar/39Ar age determination. Calcareous nannofossil assemblages allowed the attribution of the analyzed sediments to the biozone MMN21a, and the succession spans from more than 48 to ca. 105 ka, although there are at least two stratigraphic gaps and minor resedimentation episodes. A paleoclimatic reconstruction obtained via δ18O and planktonic foraminifera data identifies several of the major climatic events that occurred in the investigated time span. A total of 13 visible tephra layers and cryptotephras are recognized and correlated with their volcanic sources (Campanian Volcanic Zone and Pantelleria volcano) and, when possible, with well-known and dated events or with widespread marker tephras. Using a combination of biostratigraphic, oxygen isotope, and tephrostratigraphic correlations, we develop a chronostratigraphy for the main climatic events and tephra layers in the core and pinpoint tephra markers for the climatic events comprised between GS 22 and GI 24 in the eastern Tyrrhenian Sea