Signature whistle productions, development, and perception in free-ranging bottlenose dolphins

Data from behavioral observations and acoustic recordings of free-ranging bottlenose dolphins (Tursiops truncatus) were analyzed to determine whether signature whistles occur in the wild, and to determine when whistle crystallization occurs in calves. The study animals were part of a resident community of bottlenose dolphins in the waters near Sarasota, Florida. Three mother-calf pairs and their associates were recorded for a total of 141.25 hours between May and August of 1994 and 1995. Each whistle was conservatively classified into one of four categories: signature, probable signature, upsweep, or other. Overall whistle production varied significantly with both activity and group size. Whistles occurred more frequently than expected during socializing and in groups of over five dolphins, and occurred less frequently than expected during travelling and in groups of two dolphins. Signature and probable signature whistles made up approximately 52% of all whistles produced by these free-ranging bottlenose dolphins. Each of the three calves studied developed its whistle prior to four months of age. Each mother’s signature whistle comprised approximately 3% of the whistles recorded during the first year of her calf’s life, regardless of social or auditory environment, which varied greatly among the calves. One calf developed a signature whistle similar to that of its mother. To evaluate whether certain acoustic features of whistles are perceptually important to dolphins, 44 playback experiments were conducted with temporarily captured dolphins near Sarasota, Florida in June of 2000 and 2001. Response measures included number of head turns toward or away from the speaker, echolocation bouts, and whistles. In a modified habituation-dishabituation design, habituation to the first stimulus did not occur. Additionally, there were low overall response rates to most synthetic stimuli. There were no significant differences in responses to whistles containing differing numbers of loops or differing contours, with the exception that head turn rates were higher in response to modified synthetic upsweeps than to several other synthetic stimuli. In addition, whistle response rates were significantly higher to known, natural whistle stimuli than to synthetic upsweeps. These results suggest that natural whistles and modified upsweeps contain whistle features not adequately conveyed by other synthetic stimuli

Connecting the study of wild influenza with the potential for pandemic disease

Continuing outbreaks of pathogenic (H5N1) and pandemic (SOIVH1N1) influenza have underscored the need to understand the origin, characteristics, and evolution of novel influenza A virus (IAV) variants that pose a threat to human health. In the last 4–5 years, focus has been placed on the organization of large-scale surveillance programs to examine the phylogenetics of avian influenza virus (AIV) and host–virus relationships in domestic and wild animals. Here we review the current gaps in wild animal and environmental surveillance and the current understanding of genetic signatures in potentially pandemic strains.National Institute of Allergy and Infectious Diseases (U.S.) (Contract HHSN266200700010C)Massachusetts Institute of Technolog

GenoCAD for iGEM: a grammatical approach to the design of standard-compliant constructs

One of the foundations of synthetic biology is the project to develop libraries of standardized genetic parts that could be assembled quickly and cheaply into large systems. The limitations of the initial BioBrick standard have prompted the development of multiple new standards proposing different avenues to overcome these shortcomings. The lack of compatibility between standards, the compliance of parts with only some of the standards or even the type of constructs that each standard supports have significantly increased the complexity of assembling constructs from standardized parts. Here, we describe computer tools to facilitate the rigorous description of part compositions in the context of a rapidly changing landscape of physical construction methods and standards. A context-free grammar has been developed to model the structure of constructs compliant with six popular assembly standards. Its implementation in GenoCAD makes it possible for users to quickly assemble from a rich library of genetic parts, constructs compliant with any of six existing standards

Sheep Updates 2006 - part 3

This session covers six papers from different authors: GRAZING 1. Making better use of clover, Karen Venning and Andrew Thompson, Department of Primary Industries, Victoria 2. Grazing systems demonstration to optimise pasture utilisation and stocking rate, Mike Hyder, Sue-Ellen Shaw, Kelly Hill and Ron McTaggart, Department of Agriculture and Food Western Australia. 3. Know your audience to increase their rate of practice change - Lifetime Wool as an example, Gus Rose, Department of Agriculture and Food Western Australia, Carolyn Kabore, Kazresearch REPRODUCTION 4. Lifetime Wool - Ewe Management Guidlines, Mandy Curnow, Department of Agriculture and Food Western Australia 5. Achieving the best reproductive performance from your hoggets, Kenyon PR, Morris ST, West DM, Perkins NR, Pinchbeck GL., Institute of Veterinary, Animal and Biomedical Sciences, Massey University, New Zealand. 6. Lifetime Wool: Twin futures, Dr Ralph Behrendt, Department of Primary Industries, Victori

Mobilizing Community based Organizations to Address Obesity: Our Communities, Our Solutions

Purpose: The purpose of the focus groups is to: 1) explore existing thought patterns and lived experiences about eating habits and daily activity of African American (AA) children at risk for childhood obesity, 2) acquire an in-depth explanation of participants’ attitudes towards factors related to childhood obesity encompassing their social norms, dietary habits, and environmental influences, and 3) explore rationales for differences between children and parental perspectives of ideal health. Method: Using a Community Based Participatory Research (CPBR) framework, the HAUL research team, comprised of public health professionals, local policy makers, physicians, and community leaders, will facilitate 20 focus groups (10 among children and 10 among their parents) with community members accessing HAUL services. Focus group results will be used to develop a larger population based survey to further investigate obesity related risk factors. Use of Geographic Information System (GIS) mapping will identify resources (parks, fitness centers, healthy food markets, health clinics, etc.) in the residential communities of the target population. Results: At the time of submission, 6 focus groups have been completed (28 participants, 14 children, 14 adults). The primary themes identifying differences in responses of parents and children address proximity of access to healthy food choices, food type consumed by children regularly, and children’s preference for fast food versus home cooked meals. These themes will guide the development of the “UT-HAUL Healthy Houston Initiative”, a pilot program seeking to increase healthy decision making and access to wellness services among AAs. Conclusions: “UT-HAUL Healthy Houston Initiative” is the initial course of action in addressing societal factors, renowned for breeding social injustice commonplace in AA populations. Our goal is to expand the Urban League movement\u27s efficacy by identifying data that will combat social injustices while strengthening the core of the communities we serve

Drug-resistant genotypes and multi-clonality in Plasmodium falciparum analysed by direct genome sequencing from peripheral blood of malaria patients.

Naturally acquired blood-stage infections of the malaria parasite Plasmodium falciparum typically harbour multiple haploid clones. The apparent number of clones observed in any single infection depends on the diversity of the polymorphic markers used for the analysis, and the relative abundance of rare clones, which frequently fail to be detected among PCR products derived from numerically dominant clones. However, minority clones are of clinical interest as they may harbour genes conferring drug resistance, leading to enhanced survival after treatment and the possibility of subsequent therapeutic failure. We deployed new generation sequencing to derive genome data for five non-propagated parasite isolates taken directly from 4 different patients treated for clinical malaria in a UK hospital. Analysis of depth of coverage and length of sequence intervals between paired reads identified both previously described and novel gene deletions and amplifications. Full-length sequence data was extracted for 6 loci considered to be under selection by antimalarial drugs, and both known and previously unknown amino acid substitutions were identified. Full mitochondrial genomes were extracted from the sequencing data for each isolate, and these are compared against a panel of polymorphic sites derived from published or unpublished but publicly available data. Finally, genome-wide analysis of clone multiplicity was performed, and the number of infecting parasite clones estimated for each isolate. Each patient harboured at least 3 clones of P. falciparum by this analysis, consistent with results obtained with conventional PCR analysis of polymorphic merozoite antigen loci. We conclude that genome sequencing of peripheral blood P. falciparum taken directly from malaria patients provides high quality data useful for drug resistance studies, genomic structural analyses and population genetics, and also robustly represents clonal multiplicity

"Older Adults with ASD: The Consequences of Aging." Insights from a series of special interest group meetings held at the International Society for Autism Research 2016-2017

A special interest group (SIG) entitled "Older Adults with ASD: The Consequences of Aging" was held at the International Society for Autism Research (INSAR) annual meetings in 2016 and 2017. The SIG and subsequent meetings brought together, for the first time, international delegates who were members of the autistic community, researchers, practitioners and service providers. Based on aging autism research that is already underway in UK, Europe, Australia and North America, discussions focussed on conceptualising the parameters of aging when referring to autism, and the measures that are appropriate to use with older adults when considering diagnostic assessment, cognitive factors and quality of life in older age. Thus, the aim of this SIG was to progress the research agenda on current and future directions for autism research in the context of aging. A global issue on how to define 'aging' when referring to ASD was at the forefront of discussions. The ‘aging’ concept can in principle refer to all developmental transitions. However, in this paper we focus on the cognitive and physical changes that take place from mid-life onwards. Accordingly, it was agreed that aging and ASD research should focus on adults over the age of 50 years, given the high rates of co-occurring physical and mental health concerns and increased risk of premature death in some individuals. Moreover, very little is known about the cognitive change, care needs and outcomes of autistic adults beyond this age. Discussions on the topics of diagnostic and cognitive assessments, and of quality of life and well-being were explored through shared knowledge about which measures are currently being used and which background questions should be asked to obtain comprehensive and informative developmental and medical histories. Accordingly, a survey was completed by SIG delegates who were representatives of international research groups across four continents, and who are currently conducting studies with older autistic adults. Considerable overlap was identified across different research groups in measures of both autism and quality of life, which pointed to combining data and shared learnings as the logical next step. Regarding the background questions that were asked, the different research groups covered similar topics but the groups differed in the way these questions were formulated when working with autistic adults across a range of cognitive abilities. It became clear that continued input from individuals on the autism spectrum is important to ensure that questionnaires used in ongoing and future are accessible and understandable for people across the whole autistic spectrum, including those with limited verbal abilities

Gender equality, resilience to climate change, and the design of livestock projects for rural livelihoods

Currently, there is growing interest in how livestock projects can contribute to resilience to the effects of climate change. In this article we recommend a shift away from gross productivity to sustainability, via the use of thrifty local breeds, with an additional emphasis on improving survival of young animals. These animals, due to their local adaptations, are more likely to be resilient to climate change. There is a gender dimension to these proposals, since smaller animals and local breeds are more likely to be perceived by communities as suitable for husbandry by women. We recommend a re-orientation towards an explicit gender-equality focus for these projects

Quantitative Measurements of Alternating Finger Tapping in Parkinson's Disease Correlate With UPDRS Motor Disability and Reveal the Improvement in Fine Motor Control From Medication and Deep Brain Stimulation

Abstract: The Unified Parkinson's Disease Rating Scale (UP-DRS) is the primary outcome measure in most clinical trials of Parkinson's disease (PD) therapeutics. Each subscore of the motor section (UPDRS III) compresses a wide range of motor performance into a coarse-grained scale from 0 to 4; the assessment of performance can also be subjective. Quantitative digitography (QDG) is an objective, quantitative assessment of digital motor control using a computer-interfaced musical keyboard. In this study, we show that the kinematics of a repetitive alternating finger-tapping (RAFT) task using QDG correlate with the UPDRS motor score, particularly with the bradykinesia subscore, in 33 patients with PD. We show that dopaminergic medication and an average of 9.5 months of bilateral subthalamic nucleus deep brain stimulation (B-STN DBS) significantly improve UPDRS and QDG scores but may have different effects on certain kinematic parameters. This study substantiates the use of QDG to measure motor outcome in trials of PD therapeutics and shows that medication and B-STN DBS both improve fine motor control