MOLECULAR EVALUATION OF ANTISTAPHYLOCOCCAL SECONDARY METABOLITES OF BACTERIAL ISOLATES FROM HOSPITAL WASTEWATER ENVIRONMENT

Objective: Antibiotics revolutionized medicine in the 20th century, and have together with vaccination led to the near eradication of diseases such as tuberculosis in the developed world. Their effectiveness and easy access led to overuse, especially in livestock raising, prompting bacteria to develop resistance. The lantibiotics form a particular group among the antimicrobial peptides and are characterized by unique structural features. They are produced by a group of bacteria against some gram-positive bacteria. The present study was designed to screen for the lantibiotic producing organisms from the hospital samples.Methods: The bacterial isolates were identified based on Bergey's manual of bacteriology and screened for the epidermin gene by polymerase chain reaction amplification.Results: Of the 21 isolates screened, only 10 of them showed positive amplification for the epigene. Based on the biochemical characteristics, the isolates obtained were identified and labeled.Conclusion: Further study on the purification of the compound need to be done. Some bacterial samples may not have the epidermin gene which does not show amplification, this confers that the epidermin gene is absent in certain organisms

Transabdominal pre-peritoneal mesh repair versus Lichtenstein's hernioplasty

BACKGROUND: In the era of minimal invasive surgery, hernia repair has seen a paradigm shift from open to laparoscopic technique. However, superiority of laparoscopic repair over open repair is still controversial. Available literatures have shown laparoscopic technique to be better in term of post-operative pain and early return to work. METHODS: In this prospective study, a total of 102 patients aged 21-78 years with inguinal hernia were recruited and enrolled into two comparative groups, namely Lichtenstein's and Transabdominal pre-peritoneal, and each group containing 51 participants. Hospital ethical committee approval and written informed consent from patients was obtained. Primarily, the duration of operation, post-operative pain and complication, and quality of life after surgery was compared between the two different approaches of hernia repair. RESULTS: The study results demonstrated a statistically significant superiority of trans abdominal pre-peritoneal repair over Lichtenstein's hernioplasty in terms of post operative pain (2.00±0.63 vs 3.90±0.74 VAS score, P valu