The glycolytic enzyme phosphofructokinase-1 assembles into filaments.

Despite abundant knowledge of the regulation and biochemistry of glycolytic enzymes, we have limited understanding on how they are spatially organized in the cell. Emerging evidence indicates that nonglycolytic metabolic enzymes regulating diverse pathways can assemble into polymers. We now show tetramer- and substrate-dependent filament assembly by phosphofructokinase-1 (PFK1), which is considered the "gatekeeper" of glycolysis because it catalyzes the step committing glucose to breakdown. Recombinant liver PFK1 (PFKL) isoform, but not platelet PFK1 (PFKP) or muscle PFK1 (PFKM) isoforms, assembles into filaments. Negative-stain electron micrographs reveal that filaments are apolar and made of stacked tetramers oriented with exposed catalytic sites positioned along the edge of the polymer. Electron micrographs and biochemical data with a PFKL/PFKP chimera indicate that the PFKL regulatory domain mediates filament assembly. Quantified live-cell imaging shows dynamic properties of localized PFKL puncta that are enriched at the plasma membrane. These findings reveal a new behavior of a key glycolytic enzyme with insights on spatial organization and isoform-specific glucose metabolism in cells

Listening to a conversation with aggressive content expands the interpersonal space

The distance individuals maintain between themselves and others can be defined as ‘interpersonal space’. This distance can be modulated both by situational factors and individual characteristics. Here we investigated the influence that the interpretation of other people interaction, in which one is not directly involved, may have on a person’s interpersonal space. In the current study we measured, for the first time, whether the size of interpersonal space changes after listening to other people conversations with neutral or aggressive content. The results showed that the interpersonal space expands after listening to a conversation with aggressive content relative to a conversation with a neutral content. This finding suggests that participants tend to distance themselves from an aggressive confrontation even if they are not involved in it. These results are in line with the view of the interpersonal space as a safety zone surrounding one’s body

Enhancing Perceived Safety in Human–Robot Collaborative Construction Using Immersive Virtual Environments

Advances in robotics now permit humans to work collaboratively with robots. However, humans often feel unsafe working alongside robots. Our knowledge of how to help humans overcome this issue is limited by two challenges. One, it is difficult, expensive and time-consuming to prototype robots and set up various work situations needed to conduct studies in this area. Two, we lack strong theoretical models to predict and explain perceived safety and its influence on human–robot work collaboration (HRWC). To address these issues, we introduce the Robot Acceptance Safety Model (RASM) and employ immersive virtual environments (IVEs) to examine perceived safety of working on tasks alongside a robot. Results from a between-subjects experiment done in an IVE show that separation of work areas between robots and humans increases perceived safety by promoting team identification and trust in the robot. In addition, the more participants felt it was safe to work with the robot, the more willing they were to work alongside the robot in the future.University of Michigan Mcubed Grant: Virtual Prototyping of Human-Robot Collaboration in Unstructured Construction EnvironmentsPeer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/145620/1/You et al. forthcoming in AutCon.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145620/4/You et al. 2018.pdfDescription of You et al. 2018.pdf : Published Versio

Enfermagem moderna: a ordem do cuidado Enfermería Moderna: el orden del cuidado Modern nursing: the order of care

Com o propósito de colocar em foco a enfermagem moderna, faço neste ensaio uma leitura das condições de possibilidade do surgimento do regime de práticas da enfermagem. Apoiada por textos de Michel Foucault e identificada com os Estudos Culturais resgato fragmentos da história da enfermagem para reconstituir a organização de seu regime de práticas, num determinado momento histórico e no ambiente hospitalar. Conto a história de algumas das personagens desse enredo mostrando que o passado pode ser um produto do presente, já que encerra uma escrita da história construída e moldada pela vontade de seus autores. Destaco a enfermagem moderna como uma prática de cuidados que se profissionaliza e constrói um saber próprio que pretende assegurar independência profissional.<br>Con el propósito de colocar en foco la enfermería moderna, hago en este ensayo una lectura de las condiciones de posibilidad del surgimiento del régimen de prácticas de la enfermería. Apoyada por textos de Michel Foucault e identificada con los Estudios Culturales rescato fragmentos de la historia de la enfermería para reconstituir la organización de su régimen de prácticas, en un determinado momento histórico y en el ambiente hospitalario. Cuento la historia de algunos de los personajes de ese enredo mostrando que el pasado puede ser un producto del presente, ya que termina una escritura de la historia construida y moldeada por las ganas de sus autores. Destaco la enfermería moderna como una práctica de cuidados que se profesionaliza y construye un saber propio que pretende asegurar independencia profesional.<br>With the purpose of placing modern nursing into focus, in this essay I go over the possible conditions for the emergence of nursing practice systems. Based on texts by Michel Foucault and identified with Cultural Studies, I reclaim fragments from nursing history in order to put back together the organization of its practice system at a given historical moment and in a hospital environment. I tell the story of some of this plot's characters in an effort to show that the past can become the product of the present, once it encompasses historical writings devised and set up by the will of its authors. I highlight modern nursing as a practice of care that becomes professional and builds its own knowledge intent on ensuring professional independence