Data-Driven Development of a Roadside Safety Marketing Campaign for Tree Removal – Phase I

Since the 1970s, tree crashes have been one of the most common and deadly fixed-object fatal crash types, resulting in more than 3,000 fatal crashes and 3,500 fatalities each year. While fatal tree crashes could be prevented by removing trees adjacent to the roadway, an extensive national tree removal project would be unnecessary, cost-prohibitive, and would experience significant political resistance. The Midwest Pooled Fund Program jointly funded a research study to develop marketing methods and approaches which would focus on tree removal, replacement, or relocation in the most critical areas. Researchers conducted an extensive background investigation into tree crashes, other available studies that reviewed and analyzed tree crash data, and various state DOT and local safety-related marketing campaigns. Researchers also investigated state and local recommendations for clear zone requirements adjacent to various road classes, and issued a survey to state DOTs to obtain local perspectives of marketing and advertising plans. Finally, researchers collected over 400,000 tree and utility pole crashes from 12 different states over a five-year period to analyze the crash data and tabulate results. Draft marketing and advertising plans were developed to demonstrate the type of safety advertising techniques and messages which could be used to inform and influence the public regarding the danger of roadside trees and the importance of tree removal

CABLE-TO-POST ATTACHMENTS FOR A NON-PROPRIETARY HIGH-TENSION CABLE BARRIER – PHASE II

The research objectives reported herein were based on further development of cable-to-post attachment hardware for use in the non-proprietary high-tension cable barrier system. Specifically, this project aimed to develop and evaluate alternative attachment designs for the bolted, tabbed bracket and evaluate the use of a larger diameter, keeper rod for use in the top cable attachment. From a group of over 25 alternative attachment concepts, two tabbed bracket designs were selected for evaluation through dynamic component testing: 1) the lateral shear plate attachment and 2) the drop-in shear plate attachment. The drop-in shear plate concept did not perform as intended, as the vertical release load was too high and the lateral release loads were too low. Alternatively, the lateral shear plate design provided vertical and lateral release loads very similar to the original bolted, tabbed bracket V10. However, concerns were raised over the lack of rigidity in the attachment and the ease of installations of the shear plate. Thus, it was recommended that the lateral shear plate design be modified and reevaluated prior to use in the full-scale system. Dynamic bogie or component testing of the 3/16-in. (4.8-mm) diameter brass keeper rod provided a quick cable release similar to the previous design. The increased strength of the rod also showed promise in distributing impact loads more uniformly to adjacent posts and helping reduce system deflections. Thus, the 3/16-in. (4.8-mm) diameter brass rod was recommended for use in the new high-tension cable barrier system

Isotopen- und Spurenelementuntersuchungen von geologischen Systemen

Thermal Ionization Mass Spectrometry (TIMS) and Synchrotron X-Ray Fluoresence (SYXRF) are two methods for analyzing the isotope and trace element compositions of geological materials (e.g. rocks and fluids), in order to reconstruct their origin and history. We present several examples of how geological problems can be solved using these analytical methods: 1) age-dating of the oceanic crust beneath Gran Canaria (Canary Islands) using the Sm-Nd isotopic system, 2) tracing the origin of volcanic seamounts, ridges and ophiolites along the Pacific margin of Costa Rica with radiogenic isotope systems (Sr, Nd and Pb), 3) evaluating the role of seawater alteration on the composition of the oceanic crust using B isotopes, and 4) determining the input of halogens and other elements into the stratosphere during volcanic eruptions and assessing their effect on the ozone layer

Using a CO2 Surgical Laser for Piglet Castration to Reduce Pain and Inflammation, and to Improve Wound Healing

The objectives of this preliminary study were to determine the ability of a CO2 surgical laser to 1) reduce pain, 2) reduce inflammation, and 3) improve wound healing of piglets undergoing surgical castration. Two-day old male Yorkshire × Landrace piglets were used and randomly assigned to one of three treatments (n = 10 piglets/treatment group): surgical castration with the CO2 laser, surgical castration with a scalpel, or sham (uncastrated control). Piglets were video recorded in their pens for 1 h pre-procedure and from 0-2, 6-8, and at 24 h post-procedure for behavior scoring. Surgical site images were collected at baseline, 0, 8, 24, 48, 72, 96, 120, 144, and 168 h post-castration for wound healing assessment. Infrared thermography (IRT) images of the surgical site were also taken at baseline, 0, 0.5, 8, and 24 h post-procedure to assess inflammation. Finally, blood was collected from each piglet at baseline and 0.5 h post-castration to assess cortisol levels, prostaglandin E metabolite (PGEM), and pig-major acute phase protein (pig-MAP) concentration. Laser-castrated piglets displayed more pain behav­iors across the observation period than scalpel-castrated piglets (P = 0.049). Laser-castrated piglets also displayed significantly more agonistic behavior than both scalpel-castrated and sham piglets (P = 0.005 and P = 0.036, respectively); yet, laser-castrated piglets had significantly lower temperatures at the site of incision compared to scalpel-castrated piglets (P = 0.0211). There was no significant difference in wound healing or any of the blood parameters assessed between laser-castrated and scalpel-castrated piglets. There was evidence of thermal tissue damage on the scrotum of piglets that were castrated using the CO2 laser. This may have resulted in the unremarkable healing time and the increased pain behavior observed in this study. The surgical laser technique should be refined before conclusions can be made regarding the utility of a CO2 laser for piglet castration

Counter-current chromatography for the separation of terpenoids: A comprehensive review with respect to the solvent systems employed

Copyright @ 2014 The Authors.This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.Natural products extracts are commonly highly complex mixtures of active compounds and consequently their purification becomes a particularly challenging task. The development of a purification protocol to extract a single active component from the many hundreds that are often present in the mixture is something that can take months or even years to achieve, thus it is important for the natural product chemist to have, at their disposal, a broad range of diverse purification techniques. Counter-current chromatography (CCC) is one such separation technique utilising two immiscible phases, one as the stationary phase (retained in a spinning coil by centrifugal forces) and the second as the mobile phase. The method benefits from a number of advantages when compared with the more traditional liquid-solid separation methods, such as no irreversible adsorption, total recovery of the injected sample, minimal tailing of peaks, low risk of sample denaturation, the ability to accept particulates, and a low solvent consumption. The selection of an appropriate two-phase solvent system is critical to the running of CCC since this is both the mobile and the stationary phase of the system. However, this is also by far the most time consuming aspect of the technique and the one that most inhibits its general take-up. In recent years, numerous natural product purifications have been published using CCC from almost every country across the globe. Many of these papers are devoted to terpenoids-one of the most diverse groups. Naturally occurring terpenoids provide opportunities to discover new drugs but many of them are available at very low levels in nature and a huge number of them still remain unexplored. The collective knowledge on performing successful CCC separations of terpenoids has been gathered and reviewed by the authors, in order to create a comprehensive document that will be of great assistance in performing future purifications. © 2014 The Author(s)

A mixed treatment comparison meta-analysis of metaphylaxis treatments for bovine respiratory disease in beef cattle

Citation: Abell, K. M., Theurer, M. E., Larson, R. L., White, B. J., & Apley, M. (2017). A mixed treatment comparison meta-analysis of metaphylaxis treatments for bovine respiratory disease in beef cattle. Journal of Animal Science, 95(2), 626-635. doi:10.2527/jas2016.1062The objective of this project was to evaluate the effects of antimicrobials approved for parenteral metaphylactic use in feeder and stocker calves on morbidity and mortality for bovine respiratory disease with the use of a mixed treatment comparison meta-analysis. An initial literature review was conducted in April 2016 through Pubmed, Agricola, and CAB (Commonwealth Agricultural Bureau) for randomized controlled trials for metaphylaxis antimicrobial administered parentally to incoming feedlot or stocker calves within 48 h of arrival. The final list of publications included 29 studies, with a total of 37 trials. There were 8 different metaphylactic antimicrobials. Final event outcomes were categorized into bovine respiratory disease (BRD) morbidity cumulative incidence d 1 to <= 60 of the feeding period, BRD morbidity cumulative incidence d 1 to closeout of the feeding period, BRD mortality cumulative incidence d 1 to closeout of the feeding period, and BRD retreatment cumulative incidence morbidity d 1 to closeout of the feeding period. Network meta-analysis combined direct and indirect evidence for all the event outcomes to determine mean odds ratio (OR) with 95% credibility intervals (CrIs) for all metaphylactic antimicrobial comparisons. The "upper tier" treatment arms for morbidity d 1 to <= 60 included tulathromycin, gamithromycin, and tilmicosin. For BRD mortality cumulative incidence d 1 to closeout and BRD retreatment morbidity d 1 to closeout, classifying the treatment arms into tiers was not possible due to overlapping 95% CrIs. The results of this project accurately identified differences between metaphylactic antimicrobials, and metaphylactic antimicrobial options appear to offer different outcomes on BRD morbidity and mortality odds in feedlot cattle

Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity

Ubiquitination is initiated by transfer of ubiquitin (Ub) from a ubiquitin-activating enzyme (E1) to a ubiquitin-conjugating enzyme (E2), producing a covalently linked intermediate (E2-Ub)(1). Ubiquitin ligases (E3s) of the 'really interesting new gene' (RING) class recruit E2-Ub via their RING domain and then mediate direct transfer of ubiquitin to substrates(2). By contrast, 'homologous to E6-AP carboxy terminus' (HECT) E3 ligases undergo a catalytic cysteine-dependent transthiolation reaction with E2-Ub, forming a covalent E3-Ub intermediate(3,4). Additionally, RING-between-RING (RBR) E3 ligases have a canonical RING domain that is linked to an ancillary domain. This ancillary domain contains a catalytic cysteine that enables a hybrid RING-HECT mechanism(5). Ubiquitination is typically considered a post-translational modification of lysine residues, as there are no known human E3 ligases with non-lysine activity. Here we perform activity-based protein profiling of HECT or RBR-like E3 ligases and identify the neuron-associated E3 ligase MYCBP2 (also known as PHR1) as the apparent single member of a class of RING-linked E3 ligase with esterification activity and intrinsic selectivity for threonine over serine. MYCBP2 contains two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates. Crystallographic characterization of this class of E3 ligase, which we designate RING-Cys-relay (RCR), provides insights into its mechanism and threonine selectivity. These findings implicate non-lysine ubiquitination in cellular regulation of higher eukaryotes and suggest that E3 enzymes have an unappreciated mechanistic diversity