Affinity modulation of platelet integrin alphaIIbbeta3 by beta3-endonexin, a selective binding partner of the beta3 integrin cytoplasmic tail.

Platelet agonists increase the affinity state of integrin alphaIIbbeta3, a prerequisite for fibrinogen binding and platelet aggregation. This process may be triggered by a regulatory molecule(s) that binds to the integrin cytoplasmic tails, causing a structural change in the receptor. beta3-Endonexin is a novel 111-amino acid protein that binds selectively to the beta3 tail. Since beta3-endonexin is present in platelets, we asked whether it can affect alphaIIbbeta3 function. When beta3-endonexin was fused to green fluorescent protein (GFP) and transfected into CHO cells, it was found in both the cytoplasm and the nucleus and could be detected on Western blots of cell lysates. PAC1, a fibrinogen-mimetic mAb, was used to monitor alphaIIbbeta3 affinity state in transfected cells by flow cytometry. Cells transfected with GFP and alphaIIbbeta3 bound little or no PAC1. However, those transfected with GFP/beta3-endonexin and alphaIIbbeta3 bound PAC1 specifically in an energy-dependent fashion, and they underwent fibrinogen-dependent aggregation. GFP/beta3-endonexin did not affect levels of surface expression of alphaIIbbeta3 nor did it modulate the affinity of an alphaIIbbeta3 mutant that is defective in binding to beta3-endonexin. Affinity modulation of alphaIIbbeta3 by GFP/beta3-endonexin was inhibited by coexpression of either a monomeric beta3 cytoplasmic tail chimera or an activated form of H-Ras. These results demonstrate that beta3-endonexin can modulate the affinity state of alphaIIbbeta3 in a manner that is structurally specific and subject to metabolic regulation. By analogy, the adhesive function of platelets may be regulated by such protein-protein interactions at the level of the cytoplasmic tails of alphaIIbbeta3

RVSPY -- Radial Velocity Survey for Planets around Young Stars. Target characterization and high-cadence survey

We introduce our Radial Velocity Survey for Planets around Young stars (RVSPY), characterise our target stars, and search for substellar companions at orbital separations smaller than a few au from the host star. We use the FEROS spectrograph to obtain high signal-to-noise spectra and time series of precise radial velocities (RVs) of 111 stars most of which are surrounded by debris discs. Our target stars have spectral types between early F and late K, a median age of 400 Myr, and a median distance of 45 pc. We determine for all target stars their basic stellar parameters and present the results of the high-cadence RV survey and activity characterization. We achieve a median single-measurement RV precision of 6 m/s and derive the short-term intrinsic RV scatter of our targets (median 22 m/s), which is mostly caused by stellar activity and decays with age from >100 m/s at 500 Myr. We discover six previously unknown close companions with orbital periods between 10 and 100 days, three of which are low-mass stars, and three are in the brown dwarf mass regime. We detect no hot companion with an orbital period <10 days down to a median mass limit of ~1 M_Jup for stars younger than 500 Myr, which is still compatible with the established occurrence rate of such companions around main-sequence stars. We find significant RV periodicities between 1.3 and 4.5 days for 14 stars, which are, however, all caused by rotational modulation due to starspots. We also analyse the TESS photometric time series data and find significant periodicities for most of the stars. For 11 stars, the photometric periods are also clearly detected in the RV data. We also derive stellar rotation periods ranging from 1 to 10 days for 91 stars, mostly from TESS data. From the intrinsic activity-related short-term RV jitter, we derive the expected mass-detection thresholds for longer-period companions.Comment: 24 pages, 14 figures, 4 tables; Accepted for publication in A&

Globular cluster systems in fossil groups: NGC6482, NGC1132 and ESO306-017

We study the globular cluster (GC) systems in three representative fossil group galaxies: the nearest (NGC6482), the prototype (NGC1132) and the most massive known to date (ESO306-017). This is the first systematic study of GC systems in fossil groups. Using data obtained with the Hubble Space Telescope Advanced Camera for Surveys in the F475W and F850LP filters, we determine the GC color and magnitude distributions, surface number density profiles, and specific frequencies. In all three systems, the GC color distribution is bimodal, the GCs are spatially more extended than the starlight, and the red population is more concentrated than the blue. The specific frequencies seem to scale with the optical luminosities of the central galaxy and span a range similar to that of the normal bright elliptical galaxies in rich environments. We also analyze the galaxy surface brightness distributions to look for deviations from the best-fit S\'ersic profiles; we find evidence of recent dynamical interaction in all three fossil group galaxies. Using X-ray data from the literature, we find that luminosity and metallicity appear to correlate with the number of GCs and their mean color, respectively. Interestingly, although NGC6482 has the lowest mass and luminosity in our sample, its GC system has the reddest mean color, and the surrounding X-ray gas has the highest metallicity.Comment: 16 pages, 13 figures. Accepted for publication in A&

Two fossil groups of galaxies at z~0.4 in the COSMOS: accelerated stellar-mass build-up, different progenitors

We report on 2 fossil groups of galaxies at z=0.425 and 0.372 discovered in the Cosmic Evolution Survey (COSMOS) area. Selected as X-ray extended sources, they have total masses (M_200) of 1.9(+/-0.41)E13 and 9.5(+/-0.42)E13 M_sun, respectively, as obtained from a recent X-ray luminosity-mass scaling relation. The lower mass system appears isolated, whereas the other sits in a well-known large-scale structure (LSS) populated by 27 other X-ray emitting groups. The identification as fossil is based on the i-band photometry of all the galaxies with a photo-z consistent with that of the group at the 2-sigma confidence level and within a projected group-centric distance equal to 0.5R_200, and i_AB<=22.5-mag limited spectroscopy. Both fossil groups exhibit high stellar-to-total mass ratios compared to all the X-ray selected groups of similar mass at 0.3<=z<=0.5 in the COSMOS. At variance with the composite galaxy stellar mass functions (GSMFs) of similarly massive systems, both fossil group GSMFs are dominated by passively evolving galaxies down to M^stars~1E10 M_sun (according to the galaxy broad-band spectral energy distributions). The relative lack of star-forming galaxies with 1E10<=M^stars<=1E11 M_sun is confirmed by the galaxy distribution in the b-r vs i color-magnitude diagram. Hence, the 2 fossil groups appear as more mature than the coeval, similarly massive groups. Their overall star formation activity ended rapidly after an accelerated build up of the total stellar mass; no significant infall of galaxies with M^stars>=1E10 M_sun took place in the last 3 to 6 Gyr. This similarity holds although the 2 fossil groups are embedded in two very different density environments of the LSS, which suggests that their galaxy populations were shaped by processes that do not depend on the LSS. However, their progenitors may do so. ...Comment: 12 pages, 5 color figures, 1 table; to be published in the MNRA

The Fornax Cluster VLT Spectroscopic Survey. I – VIMOS spectroscopy of compact stellar systems in the Fornax core region

We present the results of a wide spectroscopic survey aimed at detecting extragalactic globular clusters (GCs) in the core of the Fornax cluster. About 4500 low resolution spectra (from 4800 to 10000 Å) were observed in 25 VLT/VIMOS masks covering the central 1 deg2 around the dominant galaxy NGC 1399 corresponding to ∼175 kpc galactocentric radius. We describe the methodology used for data reduction and data analysis. We found a total of 387 unique physical objects (372 GCs and 15 ultra compact dwarfs) in the field covered by our observations. Most of these objects lie in the region of NGC1399 halo, with only 10% likely belonging to other giant galaxies 48 of these objects have a literature counterpart. The new VIMOS dataset is complementary to the many GC catalogues already present in the literature and it brings the total number of tracer particles around NGC 1399 to more than 1130 objects. With this comprehensive radial velocity sample we have found that the velocity dispersion of the GC population (equally for red and blue GC populations) shows a relatively sharp increase from low velocity dispersion (∼250 − 350 km s$−1$) to high velocity dispersion (∼300 − 400 km s$−1$) at projected radius of ≈10 arcmin (∼60 kpc) from the galaxy centre. This suggests that at a projected radius of ≈60 kpc both blue and red GC populations begin to be governed by the dominating Fornax cluster potential, rather than by the central NGC 1399 galaxy potential. This kinematic evidence corroborates similar results found using surface brightness analysis and planetary nebulae kinematics

Quantification of cAMP and cGMP analogs in intact cells: pitfalls in enzyme immunoassays for cyclic nucleotides

Immunoassays are routinely used as research tools to measure intracellular cAMP and cGMP concentrations. Ideally, this application requires antibodies with high sensitivity and specificity. The present work evaluates the cross-reactivity of commercially available cyclic nucleotide analogs with two non-radioactive and one radioactive cAMP and cGMP immunoassay. Most of the tested cyclic nucleotide analogs showed low degree competition with the antibodies; however, with Rp-cAMPS, 8-Br-cGMP and 8-pCPT-cGMP, a strong cross-reactivity with the corresponding cAMP and cGMP, respectively, immunoassays was observed. The determined EIA-binding constants enabled the measurement of the intracellular cyclic nucleotide concentrations and revealed a time- and lipophilicity-dependent cell membrane permeability of the compounds in the range of 10–30% of the extracellular applied concentration, thus allowing a more accurate prediction of the intracellular analog levels in a given experiment

Radial Velocity Survey for Planets around Young Stars (RVSPY). Target characterisation and high-cadence survey

We introduce our Radial Velocity Survey for Planets around Young stars (RVSPY), characterise our target stars, and search for substellar companions at orbital separations smaller than a few au from the host star. We use the FEROS spectrograph to obtain high signal-to-noise spectra and time series of precise radial velocities (RVs) of 111 stars most of which are surrounded by debris discs. Our target stars have spectral types between early F and late K, a median age of 400 Myr, and a median distance of 45 pc. We determine for all target stars their basic stellar parameters and present the results of the high-cadence RV survey and activity characterization. We achieve a median single-measurement RV precision of 6 m/s and derive the short-term intrinsic RV scatter of our targets (median 22 m/s), which is mostly caused by stellar activity and decays with age from >100 m/s at 500 Myr. We discover six previously unknown close companions with orbital periods between 10 and 100 days, three of which are low-mass stars, and three are in the brown dwarf mass regime. We detect no hot companion with an orbital period <10 days down to a median mass limit of ~1 M_Jup for stars younger than 500 Myr, which is still compatible with the established occurrence rate of such companions around main-sequence stars. We find significant RV periodicities between 1.3 and 4.5 days for 14 stars, which are, however, all caused by rotational modulation due to starspots. We also analyse the TESS photometric time series data and find significant periodicities for most of the stars. For 11 stars, the photometric periods are also clearly detected in the RV data. We also derive stellar rotation periods ranging from 1 to 10 days for 91 stars, mostly from TESS data. From the intrinsic activity-related short-term RV jitter, we derive the expected mass-detection thresholds for longer-period companions

Extending the evolution of the stellar mass-size relation at z < 2 to low stellar mass galaxies from HFF and CANDELS

Galaxie

Star formation properties of Hickson Compact Groups based on deep Ha imaging

Galaxie