156 research outputs found
UV irradiation induces homologous recombination genes in the model archaeon, Halobacterium sp. NRC-1
BACKGROUND: A variety of strategies for survival of UV irradiation are used by cells, ranging from repair of UV-damaged DNA, cell cycle arrest, tolerance of unrepaired UV photoproducts, and shielding from UV light. Some of these responses involve UV-inducible genes, including the SOS response in bacteria and an array of genes in eukaryotes. To address the mechanisms used in the third branch of life, we have studied the model archaeon, Halobacterium sp. strain NRC-1, which tolerates high levels of solar radiation in its natural hypersaline environment. RESULTS: Cells were irradiated with 30–70 J/m(2 )UV-C and an immunoassay showed that the resulting DNA damage was largely repaired within 3 hours in the dark. Under such conditions, transcriptional profiling showed the most strongly up-regulated gene was radA1, the archaeal homolog of rad51/recA, which was induced 7-fold. Additional genes involved in homologous recombination, such as arj1 (recJ-like exonuclease), dbp (eukaryote-like DNA binding protein of the superfamily I DNA and RNA helicases), and rfa3 (replication protein A complex), as well as nrdJ, encoding for cobalamin-dependent ribonucleotide reductase involved in DNA metabolism, were also significantly induced in one or more of our experimental conditions. Neither prokaryotic nor eukaryotic excision repair gene homologs were induced and there was no evidence of an SOS-like response. CONCLUSION: These results show that homologous recombination plays an important role in the cellular response of Halobacterium sp. NRC-1 to UV damage. Homologous recombination may permit rescue of stalled replication forks, and/or facilitate recombinational repair. In either case, this provides a mechanism for the observed high-frequency recombination among natural populations of halophilic archaea
X-ray Absorption Near-Edge Structure calculations with pseudopotentials. Application to K-edge in diamond and alpha-quartz
We present a reciprocal-space pseudopotential scheme for calculating X-ray
absorption near-edge structure (XANES) spectra. The scheme incorporates a
recursive method to compute absorption cross section as a continued fraction.
The continued fraction formulation of absorption is advantageous in that it
permits the treatment of core-hole interaction through large supercells
(hundreds of atoms). The method is compared with recently developed
Bethe-Salpeter approach. The method is applied to the carbon K-edge in diamond
and to the silicon and oxygen K-edges in alpha-quartz for which polarized XANES
spectra were measured. Core-hole effects are investigated by varying the size
of the supercell, thus leading to information similar to that obtained from
cluster size analysis usually performed within multiple scattering
calculations.Comment: 11 pages, 4 figure
The high-mass star-forming region IRAS18182-1433
Submillimeter Array 1.3mm line and continuum observations toward the young
massive star-forming region IRAS18182-1433 are presented. The data are
complemented with short-spacing CO(2-1) observations and SiO(1-0) data from the
VLA. Multiple massive outflows emanate from the mm continuum peak. The CO(2-1)
data reveal a quadrupolar outflow system consisting of two outflows inclined by
\~90 degrees. One outflow exhibits a cone-like red-shifted morphology with a
jet-like blue-shifted counterpart where a blue counter-cone can only be
tentatively identified. The SiO(1-0) data suggest the presence of a third
outflow. Analyzing the 12CO/13CO line ratios indicates decreasing CO line
opacities with increasing velocities. The other seven detected molecular
species - also high-density tracers like CH3CN, CH3OH, HCOOCH3 - are all ~1-2''
offset from the mm continuum peak, but spatially associated with a strong
molecular outflow peak and a cm emission feature indicative of a thermal jet.
This spatial displacement between the molecular lines and the mm continuum
emission could be either due to an unresolved sub-source at the position of the
cm feature, or the outflow/jet itself alters the chemistry of the core
enhancing the molecular abundances toward that region. A temperature estimate
based on the CH3CN(12_k-11_k) lines suggests temperatures of the order 150K. A
velocity analysis of the high-density tracing molecules reveals that at the
given spatial resolution none of them shows any coherent velocity structure
which would be consistent with a rotating disk. We discuss this lack of
rotation signatures and attribute it to intrinsic difficulties to
observationally isolate massive accretion disks from the surrounding dense gas
envelopes and the molecular outflows.Comment: Accepted for Astronomy and Astrophysics, 13 pages 13 figures. A
version with high-resolution images can be found at
http://www.mpia.de/homes/beuther/papers.htm
Quantum Monte Carlo simulations of solids
Published versio
Angle-resolved photoemission spectroscopy of the cuprate superconductors
This paper reviews the most recent ARPES results on the cuprate
superconductors and their insulating parent and sister compounds, with the
purpose of providing an updated summary of the extensive literature in this
field. The low energy excitations are discussed with emphasis on some of the
most relevant issues, such as the Fermi surface and remnant Fermi surface, the
superconducting gap, the pseudogap and d-wave-like dispersion, evidence of
electronic inhomogeneity and nano-scale phase separation, the emergence of
coherent quasiparticles through the superconducting transition, and many-body
effects in the one-particle spectral function due to the interaction of the
charge with magnetic and/or lattice degrees of freedom. The first part of the
paper introduces photoemission spectroscopy in the context of strongly
interacting systems, along with an update on the state-of-the-art
instrumentation. The second part provides a brief overview of the scientific
issues relevant to the investigation of the low energy electronic structure by
ARPES. The rest of the paper is devoted to the review of experimental results
from the cuprates and the discussion is organized along conceptual lines:
normal-state electronic structure, interlayer interaction, superconducting gap,
coherent superconducting peak, pseudogap, electron self energy and collective
modes. Within each topic, ARPES data from the various copper oxides are
presented.Comment: Reviews of Modern Physics, in press. A HIGH-QUALITY pdf file is
available at http://www.physics.ubc.ca/~damascel/RMP_ARPES.pd
Sloan Digital Sky Survey IV: Mapping the Milky Way, Nearby Galaxies, and the Distant Universe
We describe the Sloan Digital Sky Survey IV (SDSS-IV), a project encompassing three major spectroscopic programs. The Apache Point Observatory Galactic Evolution Experiment 2 (APOGEE-2) is observing hundreds of thousands of Milky Way stars at high resolution and high signal-to-noise ratios in the near-infrared. The Mapping Nearby Galaxies at Apache Point Observatory (MaNGA) survey is obtaining spatially resolved spectroscopy for thousands of nearby galaxies (median ). The extended Baryon Oscillation Spectroscopic Survey (eBOSS) is mapping the galaxy, quasar, and neutral gas distributions between and 3.5 to constrain cosmology using baryon acoustic oscillations, redshift space distortions, and the shape of the power spectrum. Within eBOSS, we are conducting two major subprograms: the SPectroscopic IDentification of eROSITA Sources (SPIDERS), investigating X-ray AGNs and galaxies in X-ray clusters, and the Time Domain Spectroscopic Survey (TDSS), obtaining spectra of variable sources. All programs use the 2.5 m Sloan Foundation Telescope at the Apache Point Observatory; observations there began in Summer 2014. APOGEE-2 also operates a second near-infrared spectrograph at the 2.5 m du Pont Telescope at Las Campanas Observatory, with observations beginning in early 2017. Observations at both facilities are scheduled to continue through 2020. In keeping with previous SDSS policy, SDSS-IV provides regularly scheduled public data releases; the first one, Data Release 13, was made available in 2016 July
Sloan Digital Sky Survey IV: mapping the Milky Way, nearby galaxies, and the distant universe
We describe the Sloan Digital Sky Survey IV (SDSS-IV), a project encompassing three major spectroscopic programs. The Apache Point Observatory Galactic Evolution Experiment 2 (APOGEE-2) is observing hundreds of thousands of Milky Way stars at high resolution and high signal-to-noise ratios in the near-infrared. The Mapping Nearby Galaxies at Apache Point Observatory (MaNGA) survey is obtaining spatially resolved spectroscopy for thousands of nearby galaxies (median ). The extended Baryon Oscillation Spectroscopic Survey (eBOSS) is mapping the galaxy, quasar, and neutral gas distributions between and 3.5 to constrain cosmology using baryon acoustic oscillations, redshift space distortions, and the shape of the power spectrum. Within eBOSS, we are conducting two major subprograms: the SPectroscopic IDentification of eROSITA Sources (SPIDERS), investigating X-ray AGNs and galaxies in X-ray clusters, and the Time Domain Spectroscopic Survey (TDSS), obtaining spectra of variable sources. All programs use the 2.5 m Sloan Foundation Telescope at the Apache Point Observatory; observations there began in Summer 2014. APOGEE-2 also operates a second near-infrared spectrograph at the 2.5 m du Pont Telescope at Las Campanas Observatory, with observations beginning in early 2017. Observations at both facilities are scheduled to continue through 2020. In keeping with previous SDSS policy, SDSS-IV provides regularly scheduled public data releases; the first one, Data Release 13, was made available in 2016 July
Sloan Digital Sky Survey IV : mapping the Milky Way, nearby galaxies, and the distant universe
We describe the Sloan Digital Sky Survey IV (SDSS-IV), a project encompassing three major spectroscopic programs. The Apache Point Observatory Galactic Evolution Experiment 2 (APOGEE-2) is observing hundreds of thousands of Milky Way stars at high resolution and high signal-to-noise ratios in the near-infrared. The Mapping Nearby Galaxies at Apache Point Observatory (MaNGA) survey is obtaining spatially resolved spectroscopy for thousands of nearby galaxies (median z ~ 0.03). The extended Baryon Oscillation Spectroscopic Survey (eBOSS) is mapping the galaxy, quasar, and neutral gas distributions between z ~ 0.6 and 3.5 to constrain cosmology using baryon acoustic oscillations, redshift space distortions, and the shape of the power spectrum. Within eBOSS, we are conducting two major subprograms: the SPectroscopic IDentification of eROSITA Sources (SPIDERS), investigating X-ray AGNs and galaxies in X-ray clusters, and the Time Domain Spectroscopic Survey (TDSS), obtaining spectra of variable sources. All programs use the 2.5 m Sloan Foundation Telescope at the Apache Point Observatory; observations there began in Summer 2014. APOGEE-2 also operates a second near-infrared spectrograph at the 2.5 m du Pont Telescope at Las Campanas Observatory, with observations beginning in early 2017. Observations at both facilities are scheduled to continue through 2020. In keeping with previous SDSS policy, SDSS-IV provides regularly scheduled public data releases; the first one, Data Release 13, was made available in 2016 July
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