Fast analysis of relevant contaminants mixture in commercial shellfish

One of the major challenges currently faced is to develop systematic ways of addressing chemical mixtures in environmental assessment. With this purpose, a simple, rapid, and sensitive method for the detection and quantification of a mixture of relevant contaminants in molluscs has been developed. The method is based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) and Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry (UHPLC-HRMS). It includes a mixture of 23 compounds formed by pesticides, endocrine disruptors and pharmaceuticals (metolachlor, simazine, desethylatrazine, atrazine, thiabendazole, diazinon, malathion, bentazone, MCPA, propanil, acetamiprid, imidacloprid, caffeine, bisphenol A, triclosan, triclocarban, methylparaben, ethylparaben, propylparaben, 1H-benzotriazole, sulfamethoxazole, venlafaxine and carbamazepine). The method was developed and validated in 4 different types of shellfish of high commercial interest such as mussel (Mytilus galloprovincialis), oyster (Crassostrea gigas), cockle (Cerastoderma edule) and razor shell (Solen marginatus). The mean percentage of recoveries obtained for all the compounds in each mollusc type (intra-specie) ranged from 96% to 107% showing the good performance of the method developed. The relative standard deviation was under 10% for the intra-day and 17% inter-day analyses. Method detection limits and method quantification limits were below 10 ng/g dry weight for all the species and compounds targeted. Finally, the method was applied to aquaculture samples, oysters and cockles, from Ebro Delta (Spain), after some episodes of mortality occurred in 2017. A high level of bisphenol A was detected in C. edule which may explain the mortality suffered by this organism. C. gigas presented low levels of metolachlor, bentazone, acetamiprid, and methylparaben.info:eu-repo/semantics/acceptedVersio

QuEChERS-based analytical methods developed for LC-MS/MS multiresidue determination of pesticides in representative crop fatty matrices: Olives and sunflower seeds

Oilseed crops are greatly extended all over the world. Their high fat content can interfere during pesticide multiresidue analysis through liquid chromatography-tandem mass spectrometry (LC-MS/MS). This work aimed at overcoming this issue by developing and validating two QuEChERS-based methods for LC-MS/MS determination of 42 pesticides in two fatty food matrices: olives and sunflower seeds. Optimization of the extraction method was achieved following a 26-2 fractional factorial design in a highly cost-effective way. Validation of the multi-residue methods demonstrated improved limits of detection, below the established maximum residue levels (MRLs) for almost all compounds, good precision, and trueness, in compliance with SANTE guidelines. Application of these methods to the analysis of real samples from the Iberian Peninsula showed the presence of some pesticides of relevant environmental concern, including four compounds contained in the Pesticide Action Network International list of highly hazardous pesticides, found at levels between 0.03 ng/g and 104 ng/g

Determination of steroid estrogens in wastewater by high performance liquid chromatography-tandem mass spectrometry

This paper discusses the requirement for, and presents an analytical procedure for, the determination of four unconjugated steroid hormones and a conjugated steroid (estrone-3-sulfate) in wastewaters. The method quantifies the steroids by LC/MS/MS following solid phase extraction and a two stage clean-up procedure. Samples were extracted using C18 cartridges and eluates were then purified by gel permeation chromatography, followed by a further clean-up step on an aminopropyl cartridge. The limits of detection achieved were 0.2 ng l-1 for estriol, 17β-estradiol and 17α-ethinylestradiol, and 0.1 ng l-1 for estrone and the conjugate. The robustness of the method was demonstrated by achieving recoveries of >83% for all steroids in settled sewage and final effluent samples with relative standard deviations of 0.5 - 12%. The method was used to analyse a range of samples from a wastewater treatment works in south east England which demonstrated a >80% removal for estrone, estradiol and estriol with little impact on concentrations of ethinylestradiol or the conjugate

Occurrence and risk assessment of pesticides in a Mediterranean Basin with strong agricultural pressure (Guadiana Basin: Southern of Portugal)

The study aimed to assess the occurrence and the environmental risk of a group of 51 selected pesticides in the Guadiana Basin (a biodiversity hotspot, in the Mediterranean). The most abundant pesticides were bentazone and 2,4-D, while terbuthylazine together with terbutryn constituted the most ubiquitous pesticides. Eighteen out of the 38 pesticides detected are no longer approved in Europe, and 5 of them are included in the list of priority substances. The risk assessment showed that azinphos ethyl, diflufenican, irganol, imidacloprid, and oxadiazon occurred occasionally, but always in concentrations above their respective ecotoxicological threshold value. Contrary, bentazone, terbuthylazine, and terbutryn presented a high risk in most of the sampled locations and periods. The site-specific risk assessment showed a spatial and temporal pattern, with a higher risk occurring mainly in intermittent streams, in the drought period. The presence of pesticides banned from the EU market since 2009 showed the importance of improving the monitoring process, to identify the main sources of pollution and the fate of these emerging compounds. The results showed the need of implementing actions to improve the sustainable use of pesticides in agricultural areas,workingwith farmers and management entities to reduce the contamination of aquatic ecosystems. Transboundarywater governance is also required to solve potential transboundary contamination problems

Diagnóstico precoce de gestação na égua através da ultra-sonografia

Cento e onze períodos de gestação, em éguas Puro Sangue de Corrida, foram diagnosticados com ultra-som 14 dias após a última cobertura e sua evolução acompanhada até completar 50 dias. O crescimento, as alterações de forma da vesícula embrionária, a visualização do embrião e outros parâmetros a serem levados em consideração por ocasião da avaliação do desenvolvimento da gestação são aqui relatados

Diagnóstico precoce de gestação na égua através da ultra-sonografia

In this paper, one hundred and eleven gestational periods in thoroughbred mares were diagnosed by the ultrasound technique 14 days after the last mating and followed up to 50 days. The increase in size, changes in the shape of the embryonic vesicle the embryo observation and other parameters of importance for the correct evaluation of the gestacional status are described.Cento e onze períodos de gestação, em éguas Puro Sangue de Corrida, foram diagnosticados com ultra-som 14 dias após a última cobertura e sua evolução acompanhada até completar 50 dias. O crescimento, as alterações de forma da vesícula embrionária, a visualização do embrião e outros parâmetros a serem levados em consideração por ocasião da avaliação do desenvolvimento da gestação são aqui relatados

Effects of uterine flushing on post-partum conception rate in throughbred mares

Avaliou-se o efeito da lavagem uterina pós-parto com solução de NaCl a 0,9% ou iodo-povidine a 0,4% em éguas Puro-Sangue de Corrida (PSC), em relação aos índices de concepção pós-parto (p.p.). Foram comparadas as taxas de concepção de 27 éguas, sem qualquer manipulação uterina (Controle), 57 éguas com solução de NaCl (I), 36 éguas com iodopovidine (II) e 15 éguas apresentando retenção de placenta, que sofreram lavagem uterina com as duas soluções. Concluiu-se que as lavagens uterinas p.p., independentemente da substância utilizada, influenciaram negativamente a involução e a preparação do útero para a concepção, só estando indicadas em éguas com retenção de placenta, desde que aplicadas até o 3º. ou 4º. dia p.p.The effect of uterine lavage with a 0.9% NaCl or 0.4% povidine iodine solution was evaluated on the foal heat conception rate of 93 thoroughbred mares. Their conception rate were compared to the conception rate of 27 Thoroughbred mares without any manipulation of the uterus. Another 15 mares with p.p. retained placenta were submited to uterine lavage with the same solutions and their reproductive performance were accompanied. It was observed that the p.p. uterine lavage, independent of the solution used, impaired the uterine involution and the conception rates; because of its inflammatory action it is suggested that the uterine lavage could be indicated only in mares with retained placenta and, if the procedure is performed until day 3 or 4 after foaling

Synapsin II Is Involved in the Molecular Pathway of Lithium Treatment in Bipolar Disorder

Bipolar disorder (BD) is a debilitating psychiatric condition with a prevalence of 1–2% in the general population that is characterized by severe episodic shifts in mood ranging from depressive to manic episodes. One of the most common treatments is lithium (Li), with successful response in 30–60% of patients. Synapsin II (SYN2) is a neuronal phosphoprotein that we have previously identified as a possible candidate gene for the etiology of BD and/or response to Li treatment in a genome-wide linkage study focusing on BD patients characterized for excellent response to Li prophylaxis. In the present study we investigated the role of this gene in BD, particularly as it pertains to Li treatment. We investigated the effect of lithium treatment on the expression of SYN2 in lymphoblastoid cell lines from patients characterized as excellent Li-responders, non-responders, as well as non-psychiatric controls. Finally, we sought to determine if Li has a cell-type-specific effect on gene expression in neuronal-derived cell lines. In both in vitro models, we found SYN2 to be modulated by the presence of Li. By focusing on Li-responsive BD we have identified a potential mechanism for Li response in some patients

La Prospección de placeres del oro y otros minerales densos

[eng] Alluvial prospecting is the most widely used method in heavy mineral exploration. Heavy minerals are resistant to meteoric agents. Economically, the most important heavy minerals are: precious metals (gold, silver and platinum group minerals), gems (diamonds, rubies, sapphires, emeralds, etc), the tin base metal group (cassiterite), technologic metals such as titanium (ilmenite and rutile), tungsten (wolframite and scheelite), zirconium (zircon) and the rare earths. The most usual techniques used in alluvial prospecting and the different criteria employed in exploration, mineral benefit and evaluation of alluvial ore deposits known as placers are presented.[cat] La prospecció al.luvionar es un mètode excepcional per la recerca de minerals de interès econòmic, com los minerals densos d'elevada resistència física i química. Entre ells son d' interès els metalls preciosos (or, plata i platí), gemmes (diamant, rubí, zèfirs, maragdes, aigua-marina, etc.), metalls de base com l'estany (cassiterites), minerals tecnològics com el titani (ilmenites i rutils), wolfram (volframita i scheelita), zirconi (zircó), de terres rares (monacites), etc.. En aquest text, és presenten las tècniques mas usuals en Prospecció al.luvionar com la batea, així com els distints criteris a seguir per dur a termini una prospecció, un benefici i una avaluació dels seus jaciments que es coneixen com ¿placeres¿.[spa] La prospección aluvionar es un método excepcional para la búsqueda de minerales de interés económico, como los minerales densos de elevada resistencia físico-química. Entre ellos son de interés los metales preciosos (oro, plata y platinoides), gemas (diamante, rubíes, zafiros, esmeraldas, aguamarinas, etc.), metales de base como el estaño (casiteritas), minerales tecnológicos como el titanio (ilmenitas y rutilos), wólfram (volframita y scheelita), circonio (circón), de tierras raras (monacitas), etc.. En este texto, se presentan las técnicas mas usuales en prospección aluvionar como la batea, así como los distintos criterios a seguir para llevar a cabo una prospección, un beneficio y una evaluación de sus yacimientos que se conocen como ¿placeres¿