Alien Registration- Lennon, Stephen E. (Fort Fairfield, Aroostook County)

https://digitalmaine.com/alien_docs/36513/thumbnail.jp

Denitrification by sulfur-oxidizing bacteria in a eutrophic lake

Understanding the mechanistic controls of microbial denitrification is of central importance to both environmental microbiology and ecosystem ecology. Loss of nitrate (NO3 −) is often attributed to carbon-driven (heterotrophic) denitrification. However, denitrification can also be coupled to sulfur (S) oxidation by chemolithoautotrophic bacteria. In the present study, we used an in situ stable isotope (15NO3 −) tracer addition in combination with molecular approaches to understand the contribution of sulfur-oxidizing bacteria to the reduction of NO3 − in a eutrophic lake. Samples were incubated across a total dissolved sulfide (H2S) gradient (2 to 95 μM) between the lower epilimnion and the upper hypolimnion. Denitrification rates were low at the top of the chemocline (4.5 m) but increased in the deeper waters (5.0 and 5.5 m), where H2S was abundant. Concomitant with increased denitrification at depths with high sulfide was the production of sulfate (SO4 2−), suggesting that the added NO3 − was used to oxidize H2S to SO4 2−. Alternative nitrate removal pathways, including dissimilatory nitrate reduction to ammonium (DNRA) and anaerobic ammonium oxidation (anammox), did not systematically change with depth and accounted for 1 to 15% of the overall nitrate loss. Quantitative PCR revealed that bacteria of the Sulfurimonas genus that are known denitrifiers increased in abundance in response to NO3 − addition in the treatments with higher H2S. Stoichiometric estimates suggest that H2S oxidation accounted for more than half of the denitrification at the depth with the highest sulfide concentration. The present study provides evidence that microbial coupling of S and nitrogen (N) cycling is likely to be important in eutrophic freshwater ecosystems

STIS UV spectroscopy of early B supergiants in M31

We analyze STIS spectra in the 1150-1700 Angstrom wavelength range obtained for six early B supergiants in the neighboring galaxy M31. Because of their likely high (nearly solar) abundance, these stars were originally chosen to be directly comparable to their Galactic counterparts, and represent a much-needed addition to our current sample of B-type supergiants, in our efforts to study the dependence of the Wind Momentum-Luminosity Relationship on spectral type and metallicity. As a first step to determine wind momenta we fit the P-Cygni profiles of the resonance lines of N V, Si IV and C IV with standard methods, and derive terminal velocities for all of the STIS targets. From these lines we also derive ionic stellar wind column densities. Our results are compared with those obtained previously in Galactic supergiants, and confirm earlier claims of `normal' wind line intensities and terminal velocities in M31. For half of the sample we find evidence for an enhanced maximum turbulent velocity when compared to Galactic counterparts.Comment: 15 pages, 9 figures, 2 tables. Accepted for publication in The Astrophysical Journa

LGP2 plays a critical role in sensitizing mda-5 to activation by double-stranded RNA.

The DExD/H box RNA helicases retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation associated gene-5 (mda-5) sense viral RNA in the cytoplasm of infected cells and activate signal transduction pathways that trigger the production of type I interferons (IFNs). Laboratory of genetics and physiology 2 (LGP2) is thought to influence IFN production by regulating the activity of RIG-I and mda-5, although its mechanism of action is not known and its function is controversial. Here we show that expression of LGP2 potentiates IFN induction by polyinosinic-polycytidylic acid [poly(I:C)], commonly used as a synthetic mimic of viral dsRNA, and that this is particularly significant at limited levels of the inducer. The observed enhancement is mediated through co-operation with mda-5, which depends upon LGP2 for maximal activation in response to poly(I:C). This co-operation is dependent upon dsRNA binding by LGP2, and the presence of helicase domain IV, both of which are required for LGP2 to interact with mda-5. In contrast, although RIG-I can also be activated by poly(I:C), LGP2 does not have the ability to enhance IFN induction by RIG-I, and instead acts as an inhibitor of RIG-I-dependent poly(I:C) signaling. Thus the level of LGP2 expression is a critical factor in determining the cellular sensitivity to induction by dsRNA, and this may be important for rapid activation of the IFN response at early times post-infection when the levels of inducer are low

Supernova Remnants in the Sedov Expansion Phase: Thermal X-Ray Emission

Improved calculations of X-ray spectra for supernova remnants (SNRs) in the Sedov-Taylor phase are reported, which for the first time include reliable atomic data for Fe L-shell lines. This new set of Sedov models also allows for a partial collisionless heating of electrons at the blast wave and for energy transfer from ions to electrons through Coulomb collisions. X-ray emission calculations are based on the updated Hamilton-Sarazin spectral model. The calculated X-ray spectra are succesfully interpreted in terms of three distribution functions: the electron temperature and ionization timescale distributions, and the ionization timescale averaged electron temperature distribution. The comparison of Sedov models with a frequently used single nonequilibrium ionization (NEI) timescale model reveals that this simple model is generally not an appropriate approximation to X-ray spectra of SNRs. We find instead that plane-parallel shocks provide a useful approximation to X-ray spectra of SNRs, particularly for young SNRs. Sedov X-ray models described here, together with simpler plane shock and single ionization timescale models, have been implemented as standard models in the widely used XSPEC v11 spectral software package.Comment: 19 pages, 11 figures, submitted to Astrophysical Journa

The Chemical Composition of Two Supergiants in the Dwarf Irregular Galaxy WLM

[Abridged] The chemical composition of two stars in WLM has been determined from high quality UVES data obtained at the VLT UT2 (program 65.N-0375). The model atmospheres analysis shows that they have the same metallicity, [Fe/H] = -0.38 +/-0.20, and [Mg/Fe] = -0.24 +/-0.16. This result suggests that the [alpha(Mg)/Fe] ratio in WLM may be suppressed relative to solar abundances (also supported by differential abundances relative to similar stars in NGC6822 and the SMC). The absolute Mg abundance, [Mg/H] = -0.62 is high relative to what is expected from the nebulae though, where two independent spectroscopic analyses of the HII regions in WLM yield [O/H] = -0.89. Intriguingly, the oxygen abundance determined from the OI 6158 feature in one WLM star is [O/H] = -0.21 +/-0.10, corresponding to five times higher than the nebular oxygen abundance. This is the first time that a significant difference between young stellar and nebular oxygen abundances has been found, and presently, there is no simple explanation for this difference. If the stellar abundances reflect the true composition of WLM, then this galaxy lies well above the metallicity-luminosity relationship for dwarf irregular galaxies. It also suggests that WLM is more chemically evolved than currently interpreted from its color-magnitude diagram.Comment: 27 pages, 7 tables, 10 figures. accepted for publication in the Astronomical Journa

Expression of Mir-21 and Mir-143 in Cervical Specimens Ranging from Histologically Normal through to Invasive Cervical Cancer

MicroRNA expression is severely disrupted in carcinogenesis, however limited evidence is available validating results from cell-line models in human clinical cancer specimens. MicroRNA-21 (mir-21) and microRNA-143 (mir-143) have previously been identified as significantly deregulated in a range of cancers including cervical cancer. Our goal was to investigate the expression patterns of several well-studied microRNA species in cervical samples and compare the results to cell line samples.We measured the expression of mir-21 and mir-143 in 142 formalin-fixed, paraffin embedded (FFPE) cervical biopsy tissue blocks, collected from Dantec Oncology Clinic, Dakar, Senegal. MicroRNA expression analysis was performed using Taqman-based real-time PCR assays. Protein immunohistochemical staining was also performed to investigate target protein expression on 72 samples. We found that mir-21 expression increased with worsening clinical diagnosis but that mir-143 was not correlated with histology. These observations were in stark contrast to previous reports involving cervical cancer cell lines in which mir-143 was consistently down-regulated but mir-21 largely unaffected. We also identified, for the first time, that cytoplasmic expression of Programmed Cell Death Protein 4 PDCD4; a known target of mir-21) was significantly lower in women with invasive cervical carcinoma (ICC) in comparison to those with cervical intraepithelial neoplasia (2-3) or carcinoma in situ (CIN2-3/CIS), although there was no significant correlation between mir-21 and PDCD4 expression, despite previous studies identifying PDCD4 transcript as a known mir-21 target.Whilst microRNA biomarkers have a number of promising features, more studies on expression levels in histologically defined clinical specimens are required to investigate clinical relevance of discovery-based studies. Mir-21 may be of some utility in predictive screening, given that we observed a significant correlation between mir-21 expression level and worsening histological diagnosis of cervical cancer

Long-term genetic consequences of mammal reintroductions into an Australian conservation reserve

Available online 05 January 2018Reintroduction programs aim to restore self-sustaining populations of threatened species to their historic range. However, demographic restoration may not reflect genetic restoration, which is necessary for the long-term persistence of populations. Four threatened Australian mammals, the greater stick-nest rat (Leporillus conditor), greater bilby (Macrotis lagotis), burrowing bettong (Bettongia lesueur) and western barred bandicoot (Perameles bougainville), were reintroduced at Arid Recovery Reserve in northern South Australia over the last 18 years. These reintroductions have been deemed successful based on population growth and persistence, however the genetic consequences of the reintroductions are not known. We generated large single nucleotide polymorphism (SNP) datasets for each species currently at Arid Recovery and compared them to samples collected from founders. We found that average genetic diversity in all populations at the Arid Recovery Reserve are close to, or exceeding, the levels measured in the founders. Increased genetic diversity in two species was achieved by admixing slightly diverged and inbred source populations. Our results suggest that genetic diversity in translocated populations can be improved or maintained over relatively long time frames, even in small conservation reserves, and highlight the power of admixture as a tool for conservation management.Lauren C. White, Katherine E. Moseby, Vicki A. Thomson, Stephen C. Donnellan, Jeremy J. Austi

Multiple novel prostate cancer susceptibility signals identified by fine-mapping of known risk loci among Europeans

Genome-wide association studies (GWAS) have identified numerous common prostate cancer (PrCa) susceptibility loci. We have fine-mapped 64 GWAS regions known at the conclusion of the iCOGS study using large-scale genotyping and imputation in 25 723 PrCa cases and 26 274 controls of European ancestry. We detected evidence for multiple independent signals at 16 regions, 12 of which contained additional newly identified significant associations. A single signal comprising a spectrum of correlated variation was observed at 39 regions; 35 of which are now described by a novel more significantly associated lead SNP, while the originally reported variant remained as the lead SNP only in 4 regions. We also confirmed two association signals in Europeans that had been previously reported only in East-Asian GWAS. Based on statistical evidence and linkage disequilibrium (LD) structure, we have curated and narrowed down the list of the most likely candidate causal variants for each region. Functional annotation using data from ENCODE filtered for PrCa cell lines and eQTL analysis demonstrated significant enrichment for overlap with bio-features within this set. By incorporating the novel risk variants identified here alongside the refined data for existing association signals, we estimate that these loci now explain ∼38.9% of the familial relative risk of PrCa, an 8.9% improvement over the previously reported GWAS tag SNPs. This suggests that a significant fraction of the heritability of PrCa may have been hidden during the discovery phase of GWAS, in particular due to the presence of multiple independent signals within the same regio

Global wealth disparities drive adherence to COVID-safe pathways in head and neck cancer surgery

Peer reviewe