Using geomorphic and biological indicators of coastal uplift for the evaluation of paleoseismicity and

The westernmost part of the Gulf of Corinth (Greece) is an area of very fast extension (~15 mm/yr according to geodetic measurements) and active normal faulting, accompanied by intense coastal uplift and high seismicity. This study presents geomorphic and biological evidence of Holocene coastal uplift at the western extremity of the Gulf, where such evidence was previously unknown. Narrow shore platforms (benches) and rare notches occur mainly on Holocene littoral conglomerates of uplifting small fan deltas. They are perhaps the only primary paleoseismic evidence likely to provide information on earthquake recurrence at coastal faults in the specific part of the Rift system, whereas dated marine fauna can provide constraints on average Holocene coastal uplift rate. The types of geomorphic and biological evidence identified are not ideal, and there are limitations and pitfalls involved in their evaluation. In a first approach, 5 uplifted paleoshorelines may be indentified, at 0.4- 0.7, 1.0-1.3, 1.4-1.7, 2.0-2.3 and 2.8-3.4 m a.m.s.l. They probably formed after 1728 or 2250 Cal. B.P. (depending on the marine reservoir correction used in the calibration of measured radiocarbon ages). A most conservative estimate for the average coastal uplift rate during the Late Holocene is 1.6 or 1.9 mm/yr minimum (with different amounts of reservoir correction). Part of the obtained radiocarbon ages of Lithophaga sp. allows for much higher Holocene uplift rates, of the order of 3-4 mm/yr, which cannot be discarded given that similar figures exist in the bibliography on Holocene and Pleistocene uplift at neighbouring areas. They should best be cross-checked by further studies though. That the identified paleoshoreline record corresponds to episodes of coastal uplift only, cannot be demonstrated beyond all doubt by independent evidence, but it appears the most likely interpretation, given the geological and active-tectonic context and, what is known about eustatic sea-level fluctuations in the Mediterranean. Proving that the documented uplifts were abrupt (i.e., arguably coseismic), is equally difficult, but reasonably expected and rather probable. Five earthquakes in the last ca. 2000 yrs on the coastal fault zone responsible for the uplift, compare well with historical seismicity and the results of recent on-fault paleoseismological studies at the nearby Eliki fault zone. Exact amounts of coseismic uplift cannot be determined precisely, unless the rate of uniform ("regional") non-seismic uplift of Northern Peloponnesus at the specific part of the Corinth Rift is somehow constrained

Geomorphological, stratigraphic and geochronological evidence of fast Pleistocene coastal uplift in the westernmost part of the Corinth Gulf Rift (Greece)

Rapid extension and active normal faulting in the western extremity of the Corinth Gulf are accompanied by fast coastal uplift.We investigate Pleistocene uplift west of Aigion, by attempting to date remains of marine terraces and sedimentary sequences by calcareous nannoplankton and U-series analyses. Net uplift initiated recently, due to abandonment of an older rift-bounding fault zone and increase in activity on the presently active, coastal fault zone. This change apparently coincides with an abrupt slow down (or, termination) of secondary fault block tilting within the broader hangingwall block of the older zone, indicated by an angular unconformity that dates in the early part ofMIS10 ( 390–350 ka BP, preferably, in the earlier part of this period). Net uplift driven by the coastal zone resulted in the formation of MIS9c (330 ka) and younger terraces. The formation of the unconformity and the initiation of net uplift coincide temporally with a 300–400 ka unconformity recognized by recent studies in a wide area offshore Aigion i.e. they could be part of an evolutionary event that affected the entirewestern part of the Corinth Rift or, a large area therein. Uplift rate estimates at four locations are discussed with reference to the morphotectonic context of differential uplift of secondary fault blocks, and the context of possible increase in uplift ratewith time. Themost reliable and most useful estimate for uplift rate at the longitude of the studied transect is 1.74–1.85mm/year (time-averaged estimate for the last 240 ka, based on calcareous nannoplankton and sequence-stratigraphic interpretation

The Pkn22 Ser/Thr kinase in Nostoc PCC 7120: role of FurA and NtcA regulators and transcript profiling under nitrogen starvation and oxidative stress

International audienceBackground: The filamentous cyanobacterium Nostoc sp. strain PCC 7120 can fix N2 when combined nitrogen is not available. Furthermore, it has to cope with reactive oxygen species generated as byproducts of photosynthesis and respiration. We have previously demonstrated the synthesis of Ser/Thr kinase Pkn22 as an important survival response of Nostoc to oxidative damage. In this study we wished to investigate the possible involvement of this kinase in signalling peroxide stress and nitrogen deprivation. Results: Quantitative RT-PCR experiments revealed that the pkn22 gene is induced in response to peroxide stress and to combined nitrogen starvation. Electrophoretic motility assays indicated that the pkn22 promoter is recognized by the global transcriptional regulators FurA and NtcA. Transcriptomic analysis comparing a pkn22-insertion mutant and the wild type strain indicated that this kinase regulates genes involved in important cellular functions such as photosynthesis, carbon metabolism and iron acquisition. Since metabolic changes may lead to oxidative stress, we investigated whether this is the case with nitrogen starvation. Our results rather invalidate this hypothesis thereby suggesting that the function of Pkn22 under nitrogen starvation is independent of its role in response to peroxide stress. Conclusions: Our analyses have permitted a more complete functional description of Ser/Thr kinase in Nostoc. We have decrypted the transcriptional regulation of the pkn22 gene, and analysed the whole set of genes under the control of this kinase in response to the two environmental changes often encountered by cyanobacteria in their natural habitat: oxidative stress and nitrogen deprivation

Differential Phosphorylation of Ribosomal Proteins in Arabidopsis thaliana Plants during Day and Night

Protein synthesis in plants is characterized by increase in the translation rates for numerous proteins and central metabolic enzymes during the day phase of the photoperiod. The detailed molecular mechanisms of this diurnal regulation are unknown, while eukaryotic protein translation is mainly controlled at the level of ribosomal initiation complexes, which also involves multiple events of protein phosphorylation. We characterized the extent of protein phosphorylation in cytosolic ribosomes isolated from leaves of the model plant Arabidopsis thaliana harvested during day or night. Proteomic analyses of preparations corresponding to both phases of the photoperiod detected phosphorylation at eight serine residues in the C-termini of six ribosomal proteins: S2-3, S6-1, S6-2, P0-2, P1 and L29-1. This included previously unknown phosphorylation of the 40S ribosomal protein S6 at Ser-231. Relative quantification of the phosphorylated peptides using stable isotope labeling and mass spectrometry revealed a 2.2 times increase in the day/night phosphorylation ratio at this site. Phosphorylation of the S6-1 and S6-2 variants of the same protein at Ser-240 increased by the factors of 4.2 and 1.8, respectively. The 1.6 increase in phosphorylation during the day was also found at Ser-58 of the 60S ribosomal protein L29-1. It is suggested that differential phosphorylation of the ribosomal proteins S6-1, S6-2 and L29-1 may contribute to modulation of the diurnal protein synthesis in plants

Role of Plastid Protein Phosphatase TAP38 in LHCII Dephosphorylation and Thylakoid Electron Flow

Regulation of photosynthesis efficiency involves reversible phosphorylation of the light-harvesting complex through the activity of the newly identified phosphatase TAP38

Inactivation of genes coding for mitochondrial Nd7 and Nd9 complex I subunits in Chlamydomonas reinhardtii. Impact of complex I loss on respiration and energetic metabolism.

In Chlamydomonas, unlike in flowering plants, genes coding for Nd7 (NAD7/49kDa) and Nd9 (NAD9/30kDa) core subunits of mitochondrial respiratory-chain complex I are nucleus-encoded. Both genes possess all the features that facilitate their expression and proper import of the polypeptides in mitochondria. By inactivating their expression by RNA interference or insertional mutagenesis, we show that both subunits are required for complex I assembly and activity. Inactivation of complex I impairs the cell growth rate, reduces the respiratory rate, leads to lower intracellular ROS production and lower expression of ROS scavenging enzymes, and is associated to a diminished capacity to concentrate CO2 without compromising photosynthetic capacity.Peer reviewe

Inactivation of genes coding for mitochondrial Nd7 and Nd9 complex I subunits in Chlamydomonas reinhardtii. Impact of complex I loss on respiration and energetic metabolism.

In Chlamydomonas, unlike in flowering plants, genes coding for Nd7 (NAD7/49kDa) and Nd9 (NAD9/30kDa) core subunits of mitochondrial respiratory-chain complex I are nucleus-encoded. Both genes possess all the features that facilitate their expression and proper import of the polypeptides in mitochondria. By inactivating their expression by RNA interference or insertional mutagenesis, we show that both subunits are required for complex I assembly and activity. Inactivation of complex I impairs the cell growth rate, reduces the respiratory rate, leads to lower intracellular ROS production and lower expression of ROS scavenging enzymes, and is associated to a diminished capacity to concentrate CO2 without compromising photosynthetic capacity.Peer reviewe

Evaluation and mangement of fungal risk in cystic fibrosis: first results of a national French study

Date du colloque&nbsp;: 06/2009</p