Angular momentum evolution in laser-plasma accelerators

The transverse properties of an electron beam are characterized by two quantities, the emittance which indicates the electron beam extend in the phase space and the angular momentum which allows for non-planar electron trajectories. Whereas the emittance of electron beams produced in laser- plasma accelerator has been measured in several experiments, their angular momentum has been scarcely studied. It was demonstrated that electrons in laser-plasma accelerator carry some angular momentum, but its origin was not established. Here we identify one source of angular momentum growth and we present experimental results showing that the angular momentum content evolves during the acceleration

Size structure of phytoplankton biomass in the equatorial Atlantic Ocean

Abstract-The size structure of chlorophyll a (Chl a) and phaeopigments ((20, (10, (3, <2, and <1 ym) has been studied along three transects (4O, 23", and 35OW) covering the entire equatorial Atlantic Ocean, with special attention to the small sizes (<3 pm). Everywhere in the studied area, even in the equatorial upwelling, the bulk of Chl a is within organisms which pass through a 3 pm Nuclepore filter. The vertical distribution of the (1 pm Chl a is closely related to the depth of the nitracline. In the nitrate-depleted mixed layer, the (1 ym Chl a always dominates, and represents 71% of the total Chl a on the average. At the top of the nitracline, the < 1 ym Chl a concentration is maximum but represents only 50% of the total Chl a. In the nitrate-rich waters, whatever the depth, the percentage of (1 pm Chl a is everywhere (50% and decreases downwards. At the bottom of the photic zone, the < 1 prn Chl a values are nearly zero. When integrated over the whole euphotic layer, the < 1 ym Chl a represents about 25% of the total Chl a when nitrate is present at surface; this percentage reaches 60% when the top of the nitracline deepens to 100 m depth. Preliminary measurements of photosynthetic activity (light gradient and time course experiments) indicate that the < 1 ym fraction contains actively photosynthetic organisms but also organisms which are able to fix CO1 in the darkness in a significant proportion. The roles of sinking, nutrients, and light in the vertical distribution of picoplankton are discussed. Our results indicate that the size distribution of Chl a (and especially the relationship between abundance of picoplankton and nitrate distribution) is the same throughout the whole equatorial (MALONE, 1980). Recent studies have focused attention on a smaller size class of autotrophic organisms-the picoplankton ((2 pm, according to SIEBURTH et al., 1978). WATERBURY et al. (1979) and JOHNSON and SIEBURTH (1 979) show the existence of small unicellular cyanobacteria (procaryotes) with a widespread geographical distribution and the ability to achieve considerable densities. Recently, LI et al. (1983) in the eastern tropical Pacific, and PLATT et al. (1983) in the tropical North Atlantic (west of Azores) concluded that autotrophic picoplankton (< 1 prn) are capable of supplying 60% (range 20 to 80%) of the total primary production in the open ecosystem. These two last results are extremely important because they modify our concepts on the biological structure of the pelagic ecosystem, but they are limited in space and time. We .I

DIET : new developments and recent results

Among existing grid middleware approaches, one simple, powerful, and flexibleapproach consists of using servers available in different administrative domainsthrough the classic client-server or Remote Procedure Call (RPC) paradigm.Network Enabled Servers (NES) implement this model also called GridRPC.Clients submit computation requests to a scheduler whose goal is to find aserver available on the grid. The aim of this paper is to give an overview of anNES middleware developed in the GRAAL team called DIET and to describerecent developments. DIET (Distributed Interactive Engineering Toolbox) is ahierarchical set of components used for the development of applications basedon computational servers on the grid.Parmi les intergiciels de grilles existants, une approche simple, flexible et performante consiste a utiliser des serveurs disponibles dans des domaines administratifs différents à travers le paradigme classique de l’appel de procédure àdistance (RPC). Les environnements de ce type, connus sous le terme de Network Enabled Servers, implémentent ce modèle appelé GridRPC. Des clientssoumettent des requêtes de calcul à un ordonnanceur dont le but consiste àtrouver un serveur disponible sur la grille.Le but de cet article est de donner un tour d’horizon d’un intergiciel développédans le projet GRAAL appelé DIET 1. DIET (Distributed Interactive Engineering Toolbox) est un ensemble hiérarchique de composants utilisés pour ledéveloppement d’applications basées sur des serveurs de calcul sur la grille

Innate Immune Function in Placenta and Cord Blood of Hepatitis C – Seropositive Mother-Infant Dyads

Vertical transmission accounts for the majority of pediatric cases of hepatitis C viral (HCV) infection. In contrast to the adult population who develop persistent viremia in ∼80% of cases following exposure, the rate of mother-to-child transmission (2–6%) is strikingly low. Protection from vertical transmission likely requires the coordination of multiple components of the immune system. Placenta and decidua provide a direct connection between mother and infant. We hypothesized that innate immune responses would differ across the three compartments (decidua, placenta and cord blood) and that hepatitis C exposure would modify innate immunity in these tissues. The study was comprised of HCV-infected and healthy control mother and infant pairs from whom cord blood, placenta and decidua were collected with isolation of mononuclear cells. Multiparameter flow cytometry was performed to assess the phenotype, intracellular cytokine production and cytotoxicity of the cells. In keeping with a model where the maternal-fetal interface provides antiviral protection, we found a gradient in proportional frequencies of NKT and γδ-T cells being higher in placenta than cord blood. Cytotoxicity of NK and NKT cells was enhanced in placenta and placental NKT cytotoxicity was further increased by HCV infection. HCV exposure had multiple effects on innate cells including a decrease in activation markers (CD69, TRAIL and NKp44) on NK cells and a decrease in plasmacytoid dendritic cells in both placenta and cord blood of exposed infants. In summary, the placenta represents an active innate immunological organ that provides antiviral protection against HCV transmission in the majority of cases; the increased incidence in preterm labor previously described in HCV-seropositive mothers may be related to enhanced cytotoxicity of NKT cells

Tetrameric Complexes of Human Histocompatibility Leukocyte Antigen (HLA)-G Bind to Peripheral Blood Myelomonocytic Cells

The nonclassical MHC class I molecule human histocompatibility leukocyte antigen (HLA)-G is selectively expressed on fetal trophoblast tissue at the maternal–fetal interface in pregnancy. It has long been suggested that HLA-G may inhibit maternal natural killer (NK) cells through interaction with particular NK cell receptors (KIRs). To investigate interactions of HLA-G, we constructed phycoerythrin-labeled tetrameric complexes of HLA-G refolded with a self-peptide. These HLA-G tetramers failed to bind to NK cells and cells transfected with CD94/NKG2 and killer immunoglobulin-like NK receptors. In contrast, HLA-G tetramers did bind to peripheral blood monocytes, staining a CD16+CD14mid subset with greater intensity. On transfectants, HLA-G tetramers bound to inhibitory immunoglobulin-like transcript (ILT)2 and ILT4 receptors. However, staining in the presence of antibodies reactive with ILT receptors revealed that the interaction of HLA-G tetramers with blood monocytes was largely due to binding to ILT4. These results suggest that the primary role of HLA-G may be the modulation of myelomonocytic cell behavior in pregnancy

CD160-Associated CD8 T-Cell Functional Impairment Is Independent of PD-1 Expression.

Expression of co-inhibitory molecules is generally associated with T-cell dysfunction in chronic viral infections such as HIV or HCV. However, their relative contribution in the T-cell impairment remains unclear. In the present study, we have evaluated the impact of the expression of co-inhibitory molecules such as 2B4, PD-1 and CD160 on the functions of CD8 T-cells specific to influenza, EBV and CMV. We show that CD8 T-cell populations expressing CD160, but not PD-1, had reduced proliferation capacity and perforin expression, thus indicating that the functional impairment in CD160+ CD8 T cells may be independent of PD-1 expression. The blockade of CD160/CD160-ligand interaction restored CD8 T-cell proliferation capacity, and the extent of restoration directly correlated with the ex vivo proportion of CD160+ CD8 T cells suggesting that CD160 negatively regulates TCR-mediated signaling. Furthermore, CD160 expression was not up-regulated upon T-cell activation or proliferation as compared to PD-1. Taken together, these results provide evidence that CD160-associated CD8 T-cell functional impairment is independent of PD-1 expression

Surplus Photosynthetic Antennae Complexes Underlie Diagnostics of Iron Limitation in a Cyanobacterium

Chlorophyll fluorescence from phytoplankton provides a tool to assess iron limitation in the oceans, but the physiological mechanism underlying the fluorescence response is not understood. We examined fluorescence properties of the model cyanobacterium Synechocystis PCC6803 and a ΔisiA knock-out mutant of the same species grown under three culture conditions which simulate nutrient conditions found in the open ocean: (1) nitrate and iron replete, (2) limiting-iron and high-nitrate, representative of natural high-nitrate, low-chlorophyll regions, and (3) iron and nitrogen co-limiting. We show that low variable fluorescence, a key diagnostic of iron limitation, results from synthesis of antennae complexes far in excess of what can be accommodated by the iron-restricted pool of photosynthetic reaction centers. Under iron and nitrogen co-limiting conditions, there are no excess antennae complexes and variable fluorescence is high. These results help to explain the well-established fluorescence characteristics of phytoplankton in high-nutrient, low-chlorophyll ocean regions, while also accounting for the lack of these properties in low-iron, low-nitrogen regions. Importantly, our results complete the link between unique molecular consequences of iron stress in phytoplankton and global detection of iron stress in natural populations from space

Relationship of Weather Types on the Seasonal and Spatial Variability of Rainfall, Runoff, and Sediment Yield in the Western Mediterranean Basin

Rainfall is the key factor to understand soil erosion processes, mechanisms, and rates. Most research was conducted to determine rainfall characteristics and their relationship with soil erosion (erosivity) but there is little information about how atmospheric patterns control soil losses, and this is important to enable sustainable environmental planning and risk prevention. We investigated the temporal and spatial variability of the relationships of rainfall, runoff, and sediment yield with atmospheric patterns (weather types, WTs) in the western Mediterranean basin. For this purpose, we analyzed a large database of rainfall events collected between 1985 and 2015 in 46 experimental plots and catchments with the aim to: (i) evaluate seasonal differences in the contribution of rainfall, runoff, and sediment yield produced by the WTs; and (ii) to analyze the seasonal efficiency of the different WTs (relation frequency and magnitude) related to rainfall, runoff, and sediment yield. The results indicate two different temporal patterns: the first weather type exhibits (during the cold period: autumn and winter) westerly flows that produce the highest rainfall, runoff, and sediment yield values throughout the territory; the second weather type exhibits easterly flows that predominate during the warm period (spring and summer) and it is located on the Mediterranean coast of the Iberian Peninsula. However, the cyclonic situations present high frequency throughout the whole year with a large influence extended around the western Mediterranean basin. Contrary, the anticyclonic situations, despite of its high frequency, do not contribute significantly to the total rainfall, runoff, and sediment (showing the lowest efficiency) because of atmospheric stability that currently characterize this atmospheric pattern. Our approach helps to better understand the relationship of WTs on the seasonal and spatial variability of rainfall, runoff and sediment yield with a regional scale based on the large dataset and number of soil erosion experimental stations.Spanish Government (Ministry of Economy and Competitiveness, MINECO) and FEDER Projects: CGL2014 52135-C3-3-R, ESP2017-89463-C3-3-R, CGL2014-59946-R, CGL2015-65569-R, CGL2015-64284-C2-2-R, CGL2015-64284-C2-1-R, CGL2016-78075-P, GL2008-02879/BTE, LEDDRA 243857, RECARE-FP7, CGL2017-83866-C3-1-R, and PCIN-2017-061/AEI. Dhais Peña-Angulo received a “Juan de la Cierva” postdoctoral contract (FJCI-2017-33652 Spanish Ministry of Economy and Competitiveness, MEC). Ana Lucia acknowledge the "Brigitte-Schlieben-Lange-Programm". The “Geoenvironmental Processes and Global Change” (E02_17R) was financed by the Aragón Government and the European Social Fund. José Andrés López-Tarazón acknowledges the Secretariat for Universities and Research of the Department of the Economy and Knowledge of the Autonomous Government of Catalonia for supporting the Consolidated Research Group 2014 SGR 645 (RIUS- Fluvial Dynamics Research Group). Artemi Cerdà thank the funding of the OCDE TAD/CRP JA00088807. José Martínez-Fernandez acknowledges the project Unidad de Excelencia CLU-2018-04 co-funded by FEDER and Castilla y León Government. Ane Zabaleta is supported by the Hydro-Environmental Processes consolidated research group (IT1029-16, Basque Government). This paper has the benefit of the Lab and Field Data Pool created within the framework of the COST action CONNECTEUR (ES1306)

Reduction of Natural Killer but Not Effector CD8 T Lymphoyctes in Three Consecutive Cases of Severe/Lethal H1N1/09 Influenza A Virus Infection

Background: The cause of severe disease in some patients infected with pandemic influenza A virus is unclear. Methodology/Principal Findings: We present the cellular immunology profile in the blood, and detailed clinical (and postmortem) findings of three patients with rapidly progressive infection, including a pregnant patient who died. The striking finding is of reduction in natural killer (NK) cells but preservation of activated effector CD8 T lymphocytes; with viraemia in the patient who had no NK cells. Comparison with control groups suggests that the reduction of NK cells is unique to these severely ill patients. Conclusion/Significance: Our report shows markedly reduced NK cells in the three patients that we sampled and raises the hypothesis that NK may have a more significant role than T lymphocytes in controlling viral burden when the host is confronted with a new influenza A virus subtype