Nuevo método para acelerar la curación de la enfermedad de Perthes

Se presenta el caso de un paciente de 13 años de edad, con el diagnóstico de enfermedad de Perthes avanzada, atendido en el servicio de Ortopedia y Traumatología del Hospital Universitario Celia Sánchez Manduley, en el que se empleó por primera vez el tratamiento quirúrgico convencional asociado al empleo de la oxigenación hiperbárica. La evolución del paciente fue satisfactoria, la nueva combinación de tratamientos, logró reducir el período de evolución de la enfermedad en seis meses.A case of 13 years old patient with advanced Perthes disease is presented. The child was admitted to the orthopedics and traumathology service of the University Hospital Celia Sánchez Manduley. Surgical treatment was associated to hiperbaric oxynation therapy. The patient's outcome was satisfactory. The new treatment combination was able to reduce the evolution's period of disease in six months

Experimental and theoretical study of α–Eu2(MoO4)3 under compression

The compression process in the α-phase of europium trimolybdate was revised employing several experimental techniques. X-ray diffraction (using synchrotron and laboratory radiation sources), Raman scattering and photoluminescence experiments were performed up to a maximum pressure of 21 GPa. In addition, the crystal structure and Raman mode frequencies have been studied by means of first-principles density functional based methods. Results suggest that the compression process of α-Eu2(MoO4)3 can be described by three stages. Below 8 GPa, the α-phase suffers an isotropic contraction of the crystal structure. Between 8 and 12 GPa, the compound undergoes an anisotropic compression due to distortion and rotation of the MoO4 tetrahedra. At pressures above 12 GPa, the amorphization process starts without any previous occurrence of a crystalline-crystalline phase transition in the whole range of pressure. This behavior clearly differs from the process of compression and amorphization in trimolybdates with β′-phase and tritungstates with α-phase.We thank Diamond Light Source for access to beamline I15 (EE1746) that contributed to the results presented here. Part of the diffraction measurements were performed at the 'Servicio Integrado de Difraccion de Rayos X (SIDIX)' of University of La Laguna. This work has been supported by Ministerio de Economia y Competitividad of Spain (MINECO) for the research projects through the National Program of Materials (MAT2010-21270-C04-01/02/03/04, MAT2013-46649-C41/2/3/4-P and MAT2013-43319-P), the Consolider-Ingenio 2010 MALTA (CSD2007-00045), the project of Generalitat Valenciana (GVA-ACOMP/2014/243) and by the European Union FEDER funds. C Guzman-Afonso wishes to thank ACIISI and FSE for a fellowship. J A Sans thanks the FPI and 'Juan de la Cierva' programs for fellowships.Guzmán-Afonso, C.; León-Luis, S.; Sans-Tresserras, JÁ.; González -Silgo, C.; Rodríguez-Hernández, P.; Radescu, S.;  muñoz, A.... (2015). Experimental and theoretical study of α–Eu2(MoO4)3 under compression. Journal of Physics: Condensed Matter. 27(46):465401-1-465401-11. https://doi.org/10.1088/0953-8984/27/46/465401S465401-1465401-11274

Relationship between tobacco, cagA and vacA i1 virulence factors and bacterial load in patients infected by Helicobacter pylori

Background and Aim Several biological and epidemiological studies support a relationship between smoking and Helicobacter pylori (H. pylori) to increase the risk of pathology. However, there have been few studies on the potential synergistic association between specific cagA and vacA virulence factors and smoking in patients infected by Helicobacter pylori. We studied the relationship between smoking and cagA, vacA i1 virulence factors and bacterial load in H. pylori infected patients. Methods Biopsies of the gastric corpus and antrum from 155 consecutive patients in whom there was clinical suspicion of infection by H. pylori were processed. In 106 patients H. pylori infection was detected. Molecular methods were used to quantify the number of microorganisms and presence of cagA and vacA i1 genes. A standardized questionnaire was used to obtain patients’ clinical data and lifestyle variables, including tobacco and alcohol consumption. Adjusted Odds Ratios (ORadjusted) were estimated by unconditional logistic regression. Results cagA was significantly associated with active-smoking at endoscope: ORadjusted 4.52. Evidence of association was found for vacA i1 (ORadjusted 3.15). Bacterial load was higher in active-smokers, although these differences did not yield statistical significance (median of 262.2 versus 79.4 copies of H. pylori per cell). Conclusions The association between smoking and a higher risk of being infected by a virulent bacterial population and with higher bacterial load, support a complex interaction between H. pylori infection and environmental factors

Protection of Spanish Ibex (Capra pyrenaica) against Bluetongue Virus Serotypes 1 and 8 in a Subclinical Experimental Infection

Many wild ruminants such as Spanish ibex (Capra pyrenaica) are susceptible to Bluetongue virus (BTV) infection, which causes disease mainly in domestic sheep and cattle. Outbreaks involving either BTV serotypes 1 (BTV-1) and 8 (BTV-8) are currently challenging Europe. Inclusion of wildlife vaccination among BTV control measures should be considered in certain species. In the present study, four out of fifteen seronegative Spanish ibexes were immunized with a single dose of inactivated vaccine against BTV-1, four against BTV-8 and seven ibexes were non vaccinated controls. Seven ibexes (four vaccinated and three controls) were inoculated with each BTV serotype. Antibody and IFN-gamma responses were evaluated until 28 days after inoculation (dpi). The vaccinated ibexes showed significant (P<0.05) neutralizing antibody levels after vaccination compared to non vaccinated ibexes. The non vaccinated ibexes remained seronegative until challenge and showed neutralizing antibodies from 7 dpi. BTV RNA was detected in the blood of non vaccinated ibexes from 2 to the end of the study (28 dpi) and in target tissue samples obtained at necropsy (8 and 28 dpi). BTV-1 was successfully isolated on cell culture from blood and target tissues of non vaccinated ibexes. Clinical signs were unapparent and no gross lesions were found at necropsy. Our results show for the first time that Spanish ibex is susceptible and asymptomatic to BTV infection and also that a single dose of vaccine prevents viraemia against BTV-1 and BTV-8 replication

Oxidative Stress in Wild Boars Naturally and Experimentally Infected with Mycobacterium bovis

Reactive oxygen and nitrogen species (ROS-RNS) are important defence substances involved in the immune response against pathogens. An excessive increase in ROS-RNS, however, can damage the organism causing oxidative stress (OS). The organism is able to neutralise OS by the production of antioxidant enzymes (AE); hence, tissue damage is the result of an imbalance between oxidant and antioxidant status. Though some work has been carried out in humans, there is a lack of information about the oxidant/antioxidant status in the presence of tuberculosis (TB) in wild reservoirs. In the Mediterranean Basin, wild boar (Sus scrofa) is the main reservoir of TB. Wild boar showing severe TB have an increased risk to Mycobacterium spp. shedding, leading to pathogen spreading and persistence. If OS is greater in these individuals, oxidant/antioxidant balance in TB-affected boars could be used as a biomarker of disease severity. The present work had a two-fold objective: i) to study the effects of bovine TB on different OS biomarkers (namely superoxide dismutase (SOD), catalasa (CAT), glutathione peroxidase (GPX), glutathione reductase (GR) and thiobarbituric acid reactive substances (TBARS)) in wild boar experimentally challenged with Mycobacterium bovis, and ii) to explore the role of body weight, sex, population and season in explaining the observed variability of OS indicators in two populations of free-ranging wild boar where TB is common. For the first objective, a partial least squares regression (PLSR) approach was used whereas, recursive partitioning with regression tree models (RTM) were applied for the second. A negative relationship between antioxidant enzymes and bovine TB (the more severe lesions, the lower the concentration of antioxidant biomarkers) was observed in experimentally infected animals. The final PLSR model retained the GPX, SOD and GR biomarkers and showed that 17.6% of the observed variability of antioxidant capacity was significantly correlated with the PLSR X's component represented by both disease status and the age of boars. In the samples from free-ranging wild boar, however, the environmental factors were more relevant to the observed variability of the OS biomarkers than the TB itself. For each OS biomarker, each RTM was defined as a maximum by one node due to the population effect. Along the same lines, the ad hoc tree regression on boars from the population with a higher prevalence of severe TB confirmed that disease status was not the main factor explaining the observed variability in OS biomarkers. It was concluded that oxidative damage caused by TB is significant, but can only be detected in the absence of environmental variation in wild boar

Antimicrobial resistance in indicator Escherichia coli isolates from free-ranging livestock and sympatric wild ungulates in a natural environment (Northeastern Spain)

Antimicrobial resistance was assessed in indicator Escherichia coli isolates from free-ranging livestock and sympatric wild boar (Sus scrofa) and Iberian ibex (Capra pyrenaica) in a National Game Reserve in northeastern Spain. The frequency of antimicrobial resistance was low (0% to 7.9%). However, resistance to an extended-spectrum cephalosporin and fluoroquinolones was detected

Reference values of blood parameters using an immediate analysis method in Assaf ewes

13 páginas, 3 tablas, 2 figuras.[EN]Several hematologic parameters of interest in sheep production are studied. A rapid and simple method of blood analysis using whole (no heparinised) blood and a portable clinical analyzer have been used. This kind of analysis can be carried out in the farm, time of analysis being 2 minutes per sample. When results are compared to those in the bibliography, differences among breeds are revealed. Our data also suggest differences between animals within the same breed attributed to their age and physiological status. Values observed in the present study are proposed as reference values for Assaf breed animals.[ES] Se realiza un estudio de diversos parámetros sanguíneos de interés en ganado ovino y se utiliza un método de análisis rápido y sencillo utilizando sangre entera (sin anticoagulante) y un analizador clínico portátil de contrastada validez. El análisis puede realizarse en la explotación y el tiempo de análisis es de dos minutos por muestra. La comparación con los resultados de la bibliografía pone de manifiesto las diferencias entre razas, y los datos propios destacan las diferencias entre individuos en función de su edad y estado fisiológico. Se proponen valores de referencia para la raza Assaf.Este trabajo ha sido realizado por el Instituto Tecnológico Agrario de Castilla y León y el Instituto de Ganadería de Montaña (CSIC-ULE) dentro del Proyecto 2010-1287: “Producción ovina láctea adaptada a altos estándares de calidad” financiado por el Instituto Tecnológico Agrario de Castilla y León (Consejería de Agricultura y Ganadería, Junta de Castilla y León).Peer reviewe

Hematological and biochemical blood parameters in ewes and lambs assaf sheep

4 páginas, 2 tablas.-- Trabajo presentado al XXXVI Congreso de la Sociedad Española de Ovinotecnia y Caprinotecnia (SEOC) (Donostia, San Sebastián, España, 6 al 7 de octubre del 2011)[EN] In order to evaluate and characterize the main hematological (hematocrit and hemoglobin) and biochemical (pH, glucose, Na, K, Cl, PCO2, HCO3, BE, BUN, TCO2 y ANGAP) blood parameters of Assaf sheep breed, 36 ewes (at the second month of milking) and 30 lambs (one year old) were bled from the jugular vein and the obtained blood analyzed. A single standard mix of food was used to ad libitum feeding in the case of the ewes and ad libitum or restricted feeding in the case of the lambs. An I-Stat® Blood Analyser was used as a simple, fast and effective tool for the analysis of the blood parameters. Results are shown in average data, differences between groups and range (maximum and minimum) for each of the parameters analyzed.[ES] Con objeto de evaluar y caracterizar los principales parámetros hematológicos (hematocrito y hemoglobina) y bioquímicos (pH, glucosa, Na, K, Cl, PCO2, HCO3, BE, BUN, TCO2 y ANGAP) sanguíneos de ovejas de raza Assaf se extrajo sangre de la vena yugular de 36 ovejas en el segundo mes de lactación y 30 corderas de aproximadamente un año de edad. Se utilizó una ración estándar en mezcla única, ofrecida a voluntad a las ovejas y restringida y a voluntad a las corderas. Para realizar los análisis se utilizó el analizador sanguíneo I-Stat ® que permite determinar de una manera sencilla, rápida y eficaz los principales parámetros sanguíneos.Este trabajo ha sido realizado por el Instituto Tecnológico Agrario de Castilla y León y el Instituto de Ganadería de Montaña-CSIC, dentro del Proyecto titulado “Producción Ovina Láctea adaptada a altos estándares de calidad”, (PEP 2010/1287)Peer reviewe

Nd3+-doped Ca3Ga2Ge3O12 garnet: A new optical pressure sensor

The following article appeared in Journal of Applied Physics 113.21 (2013): 213517 and may be found at http://scitation.aip.org/content/aip/journal/jap/113/21/10.1063/1.4809217A pressure-induced shift of the emission spectrum corresponding to the near infrared 4F3/2 → 4I9/2 transition of Nd3+ ions in a calcium gadolinium germanium garnet was obtained in the interval from ambient conditions up to 23 GPa in order to test its suitability as an optical pressure sensor. Although several Nd3+ non-equivalent centers are present in this garnet, which complicates the assignation of the optical transitions, the R1,R2 → Z5 transitions are unequivocally characterised and fit the requirements of an ideal optical pressure sensor. Results obtained for these emission peaks indicate large pressure coefficients of -8.8 and -10.8 cm -1 GPa-1; meanwhile, the rest of the R1,R 2 → Z1-4 emissions remain almost unchanged under pressure. This behaviour is ascribed to the influence of the crystal-field at high pressure on the Z5 Stark level of the ground state and can be easily reproduced exclusively by varying the cubic term of fourth rank of the crystal-field Hamiltonian, which accounts for the Nd3+ ions and is related to medium Nd3+-oxygen distances. These coefficients are larger than those found for the R-lines of Cr3+ in ruby, -7.56 cm-1 GPa-1, suggesting that this system may be a good candidate for a luminescence pressure sensor.This work has been partially supported by MICINN (MAT2010-21270-C04-02, The MALTA Consolider CSD2007-0045 and the National Program of Infrastructure), and by EU-FEDER funds. S. F. León-Luis also wishes to thank MICINN for the FPI Grant (BES-2008-003353)