In Vivo Functional Genomic Studies of Sterol Carrier Protein-2 Gene in the Yellow Fever Mosquito

A simple and efficient DNA delivery method to introduce extrachromosomal DNA into mosquito embryos would significantly aid functional genomic studies. The conventional method for delivery of DNA into insects is to inject the DNA directly into the embryos. Taking advantage of the unique aspects of mosquito reproductive physiology during vitellogenesis and an in vivo transfection reagent that mediates DNA uptake in cells via endocytosis, we have developed a new method to introduce DNA into mosquito embryos vertically via microinjection of DNA vectors in vitellogenic females without directly manipulating the embryos. Our method was able to introduce inducible gene expression vectors transiently into F0 mosquitoes to perform functional studies in vivo without transgenic lines. The high efficiency of expression knockdown was reproducible with more than 70% of the F0 individuals showed sufficient gene expression suppression (<30% of the controls' levels). At the cohort level, AeSCP-2 expression knockdown in early instar larvae resulted in detectable phenotypes of the expression deficiency such as high mortality, lowered fertility, and distorted sex ratio after induction of AeSCP-2 siRNA expression in vivo. The results further confirmed the important role of AeSCP-2 in the development and reproduction of A. aegypti. In this study, we proved that extrachromosaomal transient expression of an inducible gene from a DNA vector vertically delivered via vitellogenic females can be used to manipulate gene expression in F0 generation. This new method will be a simple and efficient tool for in vivo functional genomic studies in mosquitoes

The Comet Interceptor Mission

Here we describe the novel, multi-point Comet Interceptor mission. It is dedicated to the exploration of a little-processed long-period comet, possibly entering the inner Solar System for the first time, or to encounter an interstellar object originating at another star. The objectives of the mission are to address the following questions: What are the surface composition, shape, morphology, and structure of the target object? What is the composition of the gas and dust in the coma, its connection to the nucleus, and the nature of its interaction with the solar wind? The mission was proposed to the European Space Agency in 2018, and formally adopted by the agency in June 2022, for launch in 2029 together with the Ariel mission. Comet Interceptor will take advantage of the opportunity presented by ESA's F-Class call for fast, flexible, low-cost missions to which it was proposed. The call required a launch to a halo orbit around the Sun-Earth L2 point. The mission can take advantage of this placement to wait for the discovery of a suitable comet reachable with its minimum ΔV capability of 600 ms-1. Comet Interceptor will be unique in encountering and studying, at a nominal closest approach distance of 1000 km, a comet that represents a near-pristine sample of material from the formation of the Solar System. It will also add a capability that no previous cometary mission has had, which is to deploy two sub-probes - B1, provided by the Japanese space agency, JAXA, and B2 - that will follow different trajectories through the coma. While the main probe passes at a nominal 1000 km distance, probes B1 and B2 will follow different chords through the coma at distances of 850 km and 400 km, respectively. The result will be unique, simultaneous, spatially resolved information of the 3-dimensional properties of the target comet and its interaction with the space environment. We present the mission's science background leading to these objectives, as well as an overview of the scientific instruments, mission design, and schedule

The Comet Interceptor Mission

Here we describe the novel, multi-point Comet Interceptor mission. It is dedicated to the exploration of a little-processed long-period comet, possibly entering the inner Solar System for the first time, or to encounter an interstellar object originating at another star. The objectives of the mission are to address the following questions: What are the surface composition, shape, morphology, and structure of the target object? What is the composition of the gas and dust in the coma, its connection to the nucleus, and the nature of its interaction with the solar wind? The mission was proposed to the European Space Agency in 2018, and formally adopted by the agency in June 2022, for launch in 2029 together with the Ariel mission. Comet Interceptor will take advantage of the opportunity presented by ESA’s F-Class call for fast, flexible, low-cost missions to which it was proposed. The call required a launch to a halo orbit around the Sun-Earth L2 point. The mission can take advantage of this placement to wait for the discovery of a suitable comet reachable with its minimum ΔV capability of 600 ms−1. Comet Interceptor will be unique in encountering and studying, at a nominal closest approach distance of 1000 km, a comet that represents a near-pristine sample of material from the formation of the Solar System. It will also add a capability that no previous cometary mission has had, which is to deploy two sub-probes – B1, provided by the Japanese space agency, JAXA, and B2 – that will follow different trajectories through the coma. While the main probe passes at a nominal 1000 km distance, probes B1 and B2 will follow different chords through the coma at distances of 850 km and 400 km, respectively. The result will be unique, simultaneous, spatially resolved information of the 3-dimensional properties of the target comet and its interaction with the space environment. We present the mission’s science background leading to these objectives, as well as an overview of the scientific instruments, mission design, and schedule

Observation of lightning-induced signals on the summit of La Grande Montagne: HF measurements

A ground-based version of the IME-HF analyzer, developed for the French TARANIS mission, was connected to a magnetic loop antenna and used for broadband measurements of lightning-induced signals in the frequency range from 5 kHz to 36 MHz. A sampling frequency of 80 MHz allows examining submicrosecond timing properties of recorded horizontal magnetic-field waveforms related to different lightning phenomena. The instrumentation is placed in a quiet electromagnetic environment of an external measurement site of the Laboratoire Souterrain à Bas Bruit (LSBB) on the summit of La Grande Montagne (1028 m, 43.94N, 5.48E). We present results of measurements recorded during two years of operation. We concentrate our attention on signals radiated by in-cloud processes which are difficult to detect in situ or optically. We also analyze a fine structure of the magnetic-field waveforms from different types of return strokes in order to investigate currents flowing in the lightning channels. After the launch of the TARANIS satellite the ground-based measurements will complement the observations from space

Share of Discontinuities in the Ozone Concentration Data from Three Reanalyses

Ozone is a very important trace gas in the stratosphere and, thus, we need to know its time evolution over the globe. However, ground-based measurements are rare, especially in the Southern Hemisphere, and while satellite observations provide broader spatial coverage generally, they are not available everywhere. On the other hand, reanalysis data have regular spatial and temporal structure, which is beneficial for trend analysis, but temporal discontinuities might exist in the data. These discontinuities may influence the results of trend studies. The aim of this paper is to detect discontinuities in ozone data of the following reanalyses: MERRA-2, ERA-5 and JRA-55 with the help of the Pettitt, the Buishand, and the Standard Normal Homogeneity tests above the 500 hPa level. The share of discontinuities varies from 30% to 70% and they are strongly layer dependent. The share of discontinuities is the lowest for JRA-55. Differences between reanalyses were found to be larger than differences between homogeneity tests within one reanalysis. Another aim of this paper is to test the ability of homogeneity tests to detect the discontinuities in 2004 and 2015, when changes in versions of satellite data took place. We showed the discontinuities in 2004 are better detected than those in 2015

Electron acceleration above thunderclouds

The work of MF andAMis sponsored by the Natural Environment Research Council (NERC) under grant NE/H024921/1. IK, OS, RL, and LU are supported by the international cooperation program of the ASCR grant M10042120 and by the GACR project 205-09-1253. JB is supported by the Earth-system project TAMOP-4.2.2.C-11/1/KONV-2012-0015 sponsored by the EU and European Social Foundation. OV is supported by the Spanish Ministry of Science and Innovation under project AYA2011-29936-C05-04. ChH acknowledges an ERC starting grant from the European Union.The acceleration of electrons results in observable electromagnetic waves which can be used for remote sensing. Here, we make use of similar to 4 Hz-66 MHz radio waves emitted by two consecutive intense positive lightning discharges to investigate their impact on the atmosphere above a thundercloud. It is found that the first positive lightning discharge initiates a sprite where electrons are accelerated during the exponential growth and branching of the sprite streamers. This preconditioned plasma above the thundercloud is subsequently exposed to a second positive lightning discharge associated with a bouncing-wave discharge. This discharge process causes a re-brightening of the existing sprite streamers above the thundercloud and initiates a subsequent relativistic electron beam.Publisher PDFPeer reviewe

The RPW Time Domain Sampler (TDS) on Solar Orbiter: In-flight performance and first data

International audienceThe Radio and Plasma Wave instrument (RPW) for Solar Orbiter includes a Time Domain Sampler sub-unit (TDS) designed to capture electromagnetic waveform measurements of high-frequency plasma waves and antenna voltage spikes associated with dust impacts. TDS will digitize three components of the electric field and one magnetic component at 524 kHz sampling rate and scan the obtained signal for plasma waves and dust impact signatures. The main science target of TDS are Langmuir waves observed in the solar wind in association with Type II and Type III solar bursts, interplanetary shocks, magnetic holes, and other phenomena. In this poster, we present the scientific data products provided by the TDS instrument and discuss the first data obtained during the commissioning phase. The first data will be used to evaluate the actual performance of the RPW TDS instrument