Tubulin cofactors and Arl2 are cage-like chaperones that regulate the soluble αβ-tubulin pool for microtubule dynamics.

Microtubule dynamics and polarity stem from the polymerization of αβ-tubulin heterodimers. Five conserved tubulin cofactors/chaperones and the Arl2 GTPase regulate α- and β-tubulin assembly into heterodimers and maintain the soluble tubulin pool in the cytoplasm, but their physical mechanisms are unknown. Here, we reconstitute a core tubulin chaperone consisting of tubulin cofactors TBCD, TBCE, and Arl2, and reveal a cage-like structure for regulating αβ-tubulin. Biochemical assays and electron microscopy structures of multiple intermediates show the sequential binding of αβ-tubulin dimer followed by tubulin cofactor TBCC onto this chaperone, forming a ternary complex in which Arl2 GTP hydrolysis is activated to alter αβ-tubulin conformation. A GTP-state locked Arl2 mutant inhibits ternary complex dissociation in vitro and causes severe defects in microtubule dynamics in vivo. Our studies suggest a revised paradigm for tubulin cofactors and Arl2 functions as a catalytic chaperone that regulates soluble αβ-tubulin assembly and maintenance to support microtubule dynamics

Originally Asymmetric Dark Matter

We propose a scenario with a fermion dark matter, where the dark matter particle used to be the Dirac fermion, but it takes the form of the Majorana fermion at a late time. The relic number density of the dark matter is determined by the dark matter asymmetry generated through the same mechanism as leptogenesis when the dark matter was the Dirac fermion. After efficient dark matter annihilation processes have frozen out, a phase transition of a scalar field takes place and generates Majorana mass terms to turn the dark matter particle into the Majorana fermion. In order to address this scenario in detail, we propose two simple models. The first one is based on the Standard Model (SM) gauge group and the dark matter originates the $SU(2)_L$ doublet Dirac fermion, analogous to the Higgsino-like neutralino in supersymmetric models. We estimate the spin-independent/dependent elastic scattering cross sections of this late-time Majorana dark matter with a proton and find the possibility to discover it by the direct and/or indirect dark matter search experiments in the near future. The second model is based on the $B-L$ gauged extension of the SM, where the dark matter is a SM singlet. Although this model is similar to the so-called Higgs portal dark matter scenario, the spin-independent elastic scattering cross section can be large enough to detect this dark matter in future experiments.Comment: 24 pages. Final journal versio

Lack of SARS Transmission among Public Hospital Workers, Vietnam

The severe acute respiratory syndrome (SARS) outbreak in Vietnam was amplified by nosocomial spread within hospital A, but no transmission was reported in hospital B, the second of two designated SARS hospitals. Our study documents lack of SARS-associated coronavirus transmission to hospital B workers, despite variable infection control measures and the use of personal protective equipment

A mutli-technique search for the most primitive CO chondrites

As part of a study to identify the most primitive COs and to look for weakly altered CMs amongst the COs, we have conducted a multi-technique study of 16 Antarctic meteorites that had been classified as primitive COs. For this study, we have determined: (1) the bulk H, C and N abundances and isotopes, (2) bulk O isotopic compositions, (3) bulk modal mineralogies, and (4) for some selected samples the abundances and compositions of their insoluble organic matter (IOM). Two of the 16 meteorites do appear to be CMs – BUC 10943 seems to be a fairly typical CM, while MIL 090073 has probably been heated. Of the COs, DOM 08006 appears to be the most primitive CO identified to date and is quite distinct from the other members of its pairing group. The other COs fall into two groups that are less primitive than DOM 08006 and ALH 77307, the previously most primitive CO. The first group is composed of members of the DOM 08004 pairing group, except DOM 08006. The second group is composed of meteorites belonging to the MIL 03377 and MIL 07099 pairing groups. These two pairing groups should probably be combined. There is a dichotomy in the bulk O isotopes between the primitive (all Antarctic finds) and the more metamorphosed COs (mostly falls). This dichotomy can only partly be explained by the terrestrial weathering experienced by the primitive Antarctic samples. It seems that the more equilibrated samples interacted to a greater extent with 16O-poor material, probably water, than the more primitive meteorites

Towards Viral Genome Annotation Standards, Report from the 2010 NCBI Annotation Workshop

Improvements in DNA sequencing technologies portend a new era in virology and could possibly lead to a giant leap in our understanding of viral evolution and ecology. Yet, as viral genome sequences begin to fill the world’s biological databases, it is critically important to recognize that the scientific promise of this era is dependent on consistent and comprehensive genome annotation. With this in mind, the NCBI Genome Annotation Workshop recently hosted a study group tasked with developing sequence, function, and metadata annotation standards for viral genomes. This report describes the issues involved in viral genome annotation and reviews policy recommendations presented at the NCBI Annotation Workshop

Face Mask Use and Control of Respiratory Virus Transmission in Households

Mask use is associated with low adherence, but adherent mask users are significantly protected against seasonal disease

A Notch updated

Cell–cell signaling mediated by the Notch receptor is iteratively involved in numerous developmental contexts, and its dysregulation has been associated with inherited genetic disorders and cancers. The core components of the signaling pathway have been identified for some time, but the study of the modulation of the pathway in different cellular contexts has revealed many layers of regulation. These include complex sugar modifications in the extracellular domain as well as transit of Notch through defined cellular compartments, including specific endosomes

Measuring the tensor to scalar ratio from CMB B-modes in presence of foregrounds

Abreg: We investigate the impact of polarized foreground emission on the performances of future CMB experiments in measuring the tensor-to-scalar ratio r. We design a component separation pipeline, based on the Smica method, aimed at estimating r and the foreground contamination from the data with no prior assumption on the frequency dependence or spatial distribution of the foregrounds. We derive error bars accounting for the uncertainty on foreground contribution. We use the current knowledge of galactic and extra-galactic foregrounds as implemented in the Planck Sky Model (PSM), to build simulations of the sky emission for various experimental setups. Our method, permits us to detect r = 0.1 at more than 3 sigma from B-modes only with Planck data, and r = 0.001 at 6 sigma for the most ambitious designs of the future EPIC probe. We find that all-sky experiments permit a proper measurement of the reionization bump despite the large scale foreground emission and are nearly insensitive to contamination from point sources and lensing if their statistical contribution can be modelled accurately. Investigating the observation of a small but clean part of the sky, we show that diffuse foregrounds remain a concern for a sensitive ground-based experiment with a limited frequency coverage when measuring r < 0.1, but are dealt with efficiently by a deep field space mission which is in return quite sensitive to lensing. Our results do not significantly depend on the overall level and frequency dependence of the diffused foreground model.Comment: 18 p. submitted to A&

Azimuthal Angle Correlations for Rapidity Separated Hadron Pairs in d+Au Collisions at sqrt(s_NN) = 200 GeV

We report on two-particle azimuthal angle correlations between charged hadrons at forward/backward (deuteron/gold going direction) rapidity and charged hadrons at mid-rapidity in deuteron-gold (d+Au) and proton-proton (p+p) collisions at sqrt(s_NN) = 200 GeV. Jet structures are observed in the correlations which we quantify in terms of the conditional yield and angular width of away side partners. The kinematic region studied here samples partons in the gold nucleus carrying nucleon momentum fraction x~0.1 to x~0.01. Within this range, we find no x dependence of the jet structure in d+Au collisions.Comment: 330 authors, 6 pages text, 4 figures, no tables. Submitted to Phys. Rev. Lett. Plain text data tables for the points plotted in figures for this and previous PHENIX publications are (or will be) publicly available at http://www.phenix.bnl.gov/papers.htm

Centrality dependence of charged hadron production in deuteron+gold and nucleon+gold collisions at sqrt(s_NN)=200 GeV

We present transverse momentum (p_T) spectra of charged hadrons measured in deuteron-gold and nucleon-gold collisions at \sqrts = 200 GeV for four centrality classes. Nucleon-gold collisions were selected by tagging events in which a spectator nucleon was observed in one of two forward rapidity detectors. The spectra and yields were investigated as a function of the number of binary nucleon-nucleon collisions, \nu, suffered by deuteron nucleons. A comparison of charged particle yields to those in p+p collisions show that the yield per nucleon-nucleon collision saturates with \nu for high momentum particles. We also present the charged hadron to neutral pion ratios as a function of p_T.Comment: 330 authors, 15 pages text, 16 figures, 3 tables. Submitted to Phys. Rev. Lett. v2 has minor changes to reflect revisions during review process. Plain text data tables for the points plotted in figures for this and previous PHENIX publications are (or will be) publicly available at http://www.phenix.bnl.gov/papers.htm