403 research outputs found
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Surfaces: An interdisciplinary project to understand and enhance health in the vulnerable rainforests of Papua New Guinea
Background
New Guinea has the third largest tropical rainforest on Earth. However, one quarter of the forests of Papua New Guinea (PNG, New Guinea’s eastern half) have been cleared or degraded, nearly half through commercial logging.Sustainable development requires supporting good health (Sustainable Development Goal [SDG] 3) and protecting life on land (SDG 15). To remote communities in PNG with low levels of health provision, these goals can seem in conflict. Logging companies’ offer of roads and income can partly extinguish the remoteness that bars access to health services, making desire for health a driver for forest destruction and erosion of health related ecosystem services. Conservation success thus requires synergies be developed with delivery of other SDGs, particularly those pertaining to health. We aim to provide a model of integrated health and conservation in PNGs rainforests.
Methods
We are mapping and piloting biological, anthropological, and medical methods to address SDGs on health and biodiversity, focusing first on scabies and fungal diseases. At Wanang, team members have a long term collaboration with nine clans with unmet health needs who collectively chose to preserve their 10,000 hectare forest whilst surrounding communities allowed logging. Similar collaborations are being developed along an altitudinal transect on Mt.Wilheim (4,509m). Stage 1 of Surfaces will (i) systematically map evidence on integrated conservation and health programmes, (ii) conduct clinical examinations and rapid anthropological assessments to understand medical needs, and survey skin disease, and (iii) produce a case study of the Wanang agreement, based on interviews with participants. This will lay the foundation for a multi-year health intervention and interdisciplinary study.
Findings
We are in the projects’ early stages (so do not yet have findings), and would appreciate advice and suggestions of collaboration from others in the Planetary Health community.
Funding
Sussex Sustainability Research Programme, University of Sussex, UK.
Contributions
All authors have commented on multiple drafts and approved the final version of the abstract for publication.
Conflicts of interest
We declare we have no conflicts of interest.
Acknowledgments
We thank the projects partner communities; New Guinea Binatang Research Centre; and our advisory group
Ability-Based Methods for Personalized Keyboard Generation
This study introduces an ability-based method for personalized keyboard
generation, wherein an individual's own movement and human-computer interaction
data are used to automatically compute a personalized virtual keyboard layout.
Our approach integrates a multidirectional point-select task to characterize
cursor control over time, distance, and direction. The characterization is
automatically employed to develop a computationally efficient keyboard layout
that prioritizes each user's movement abilities through capturing directional
constraints and preferences. We evaluated our approach in a study involving 16
participants using inertial sensing and facial electromyography as an access
method, resulting in significantly increased communication rates using the
personalized keyboard (52.0 bits/min) when compared to a generically optimized
keyboard (47.9 bits/min). Our results demonstrate the ability to effectively
characterize an individual's movement abilities to design a personalized
keyboard for improved communication. This work underscores the importance of
integrating a user's motor abilities when designing virtual interfaces.Comment: 20 pages, 7 figure
Microguards and micromessengers of the genome
The regulation of gene expression is of fundamental importance to maintain organismal function and integrity and requires a multifaceted and highly ordered sequence of events. The cyclic nature of gene expression is known as ‘transcription dynamics’. Disruption or perturbation of these dynamics can result in significant fitness costs arising from genome instability, accelerated ageing and disease. We review recent research that supports the idea that an important new role for small RNAs, particularly microRNAs (miRNAs), is in protecting the genome against short-term transcriptional fluctuations, in a process we term ‘microguarding’. An additional emerging role for miRNAs is as ‘micromessengers’—through alteration of gene expression in target cells to which they are trafficked within microvesicles. We describe the scant but emerging evidence that miRNAs can be moved between different cells, individuals and even species, to exert biologically significant responses. With these two new roles, miRNAs have the potential to protect against deleterious gene expression variation from perturbation and to themselves perturb the expression of genes in target cells. These interactions between cells will frequently be subject to conflicts of interest when they occur between unrelated cells that lack a coincidence of fitness interests. Hence, there is the potential for miRNAs to represent both a means to resolve conflicts of interest, as well as instigate them. We conclude by exploring this conflict hypothesis, by describing some of the initial evidence consistent with it and proposing new ideas for future research into this exciting topic
Health service needs and perspectives of remote forest communities in Papua New Guinea: study protocol for combined clinical and rapid anthropological assessments with parallel treatment of urgent cases
Introduction
Our project follows community requests for health service incorporation into conservation collaborations in the rainforests of Papua New Guinea (PNG). This protocol is for health needs assessments, our first step in coplanning medical provision in communities with no existing health data.
Methods and analysis
The study includes clinical assessments and rapid anthropological assessment procedures (RAP) exploring the health needs and perspectives of partner communities in two areas, conducted over 6 weeks fieldwork. First, in Wanang village (population c.200), which is set in lowland rainforest. Second, in six communities (population c.3000) along an altitudinal transect up the highest mountain in PNG, Mount Wilhelm. Individual primary care assessments incorporate physical examinations and questioning (providing qualitative and quantitative data) while RAP includes focus groups, interviews and field observations (providing qualitative data). Given absence of in-community primary care, treatments are offered alongside research activity but will not form part of the study. Data are collected by a research fellow, primary care clinician and two PNG research technicians. After quantitative and qualitative analyses, we will report: ethnoclassifications of disease, causes, symptoms and perceived appropriate treatment; community rankings of disease importance and service needs; attitudes regarding health service provision; disease burdens and associations with altitudinal-related variables and cultural practices. To aid wider use study tools are in online supplemental file, and paper and ODK versions are available free from the corresponding author.
Ethics and dissemination
Challenges include supporting informed consent in communities with low literacy and diverse cultures, moral duties to provide treatment alongside research in medically underserved areas while minimising risks of therapeutic misconception and inappropriate inducement, and PNG research capacity building. Brighton and Sussex Medical School (UK), PNG Institute of Medical Research and PNG Medical Research Advisory Committee have approved the study. Dissemination will be via journals, village meetings and plain language summaries
Zeptomole Electrochemical Detection of Metallothioneins
Thiol-rich peptides and proteins possess a large number of biological activities and may serve as markers for numerous health problems including cancer. Metallothionein (MT), a small molecular mass protein rich in cysteine, may be considered as one of the promising tumour markers. The aim of this paper was to employ chronopotentiometric stripping analysis (CPSA) for highly sensitive detection of MT.In this study, we used adsorptive transfer stripping technique coupled with CPSA for detection of cysteine, glutathione oxidized and reduced, phytochelatin, bovine serum albumin, and metallothionein. Under the optimal conditions, we were able to estimate detection limits down to tens of fg per ml. Further, this method was applied to detect metallothioneins in blood serum obtained from patients with breast cancer and in neuroblastoma cells resistant and sensitive to cisplatin in order to show the possible role of metallothioneins in carcinogenesis. It was found that MT level in blood serum was almost twice higher as compared to the level determined in healthy individuals.This paper brings unique results on the application of ultra-sensitive electroanalytical method for metallothionein detection. The detection limit and other analytical parameters are the best among the parameters of other techniques. In spite of the fact that the paper is mainly focused on metallothionein, it is worth mentioning that successful detection of other biologically important molecules is possible by this method. Coupling of this method with simple isolation methods such as antibody-modified paramagnetic particles may be implemented to lab-on-chip instrument
A framework to assess the quality and impact of bioinformatics training across ELIXIR.
ELIXIR is a pan-European intergovernmental organisation for life science that aims to coordinate bioinformatics resources in a single infrastructure across Europe; bioinformatics training is central to its strategy, which aims to develop a training community that spans all ELIXIR member states. In an evidence-based approach for strengthening bioinformatics training programmes across Europe, the ELIXIR Training Platform, led by the ELIXIR EXCELERATE Quality and Impact Assessment Subtask in collaboration with the ELIXIR Training Coordinators Group, has implemented an assessment strategy to measure quality and impact of its entire training portfolio. Here, we present ELIXIR's framework for assessing training quality and impact, which includes the following: specifying assessment aims, determining what data to collect in order to address these aims, and our strategy for centralised data collection to allow for ELIXIR-wide analyses. In addition, we present an overview of the ELIXIR training data collected over the past 4 years. We highlight the importance of a coordinated and consistent data collection approach and the relevance of defining specific metrics and answer scales for consortium-wide analyses as well as for comparison of data across iterations of the same course
Spatial covariance of herbivorous and predatory guilds of forest canopy arthropods along a latitudinal gradient
In arthropod community ecology, species richness studies tend to be prioritised over those investigating patterns of abundance. Consequently, the biotic and abiotic drivers of arboreal arthropod abundance are still relatively poorly known. In this cross-continental study, we employ a theoretical framework in order to examine patterns of covariance among herbivorous and predatory arthropod guilds. Leaf-chewing and leaf-mining herbivores, and predatory ants and spiders, were censused on > 1000 trees in nine 0.1 ha forest plots. After controlling for tree size and season, we found no negative pairwise correlations between guild abundances per plot, suggestive of weak signals of both inter-guild competition and top-down regulation of herbivores by predators. Inter-guild interaction strengths did not vary with mean annual temperature, thus opposing the hypothesis that biotic interactions intensify towards the equator. We find evidence for the bottom-up limitation of arthropod abundances via resources and abiotic factors, rather than for competition and predation.publishedVersio
The case for open science: rare diseases.
The premise of Open Science is that research and medical management will progress faster if data and knowledge are openly shared. The value of Open Science is nowhere more important and appreciated than in the rare disease (RD) community. Research into RDs has been limited by insufficient patient data and resources, a paucity of trained disease experts, and lack of therapeutics, leading to long delays in diagnosis and treatment. These issues can be ameliorated by following the principles and practices of sharing that are intrinsic to Open Science. Here, we describe how the RD community has adopted the core pillars of Open Science, adding new initiatives to promote care and research for RD patients and, ultimately, for all of medicine. We also present recommendations that can advance Open Science more globally
Arthropod distribution in a tropical rainforest: tackling a four dimensional puzzle
Quantifying the spatio-temporal distribution of arthropods in tropical rainforests represents a first step towards scrutinizing the global distribution of biodiversity on Earth. To date most studies have focused on narrow taxonomic groups or lack a design that allows partitioning of the components of diversity. Here, we consider an exceptionally large dataset (113,952 individuals representing 5,858 species), obtained from the San Lorenzo forest in Panama, where the phylogenetic breadth of arthropod taxa was surveyed using 14 protocols targeting the soil, litter, understory, lower and upper canopy habitats, replicated across seasons in 2003 and 2004. This dataset is used to explore the relative influence of horizontal, vertical and seasonal drivers of arthropod distribution in this forest. We considered arthropod abundance, observed and estimated species richness, additive decomposition of species richness, multiplicative partitioning of species diversity, variation in species composition, species turnover and guild structure as components of diversity. At the scale of our study (2km of distance, 40m in height and 400 days), the effects related to the vertical and seasonal dimensions were most important. Most adult arthropods were collected from the soil/litter or the upper canopy and species richness was highest in the canopy. We compared the distribution of arthropods and trees within our study system. Effects related to the seasonal dimension were stronger for arthropods than for trees. We conclude that: (1) models of beta diversity developed for tropical trees are unlikely to be applicable to tropical arthropods; (2) it is imperative that estimates of global biodiversity derived from mass collecting of arthropods in tropical rainforests embrace the strong vertical and seasonal partitioning observed here; and (3) given the high species turnover observed between seasons, global climate change may have severe consequences for rainforest arthropods1012CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQSolVin-Solvay SA; Smithsonian Institution; Smithsonian Tropical Research Institute; United Nations Environment Programme; Smithsonian Institution; Smithsonian National Museum of Natural History; European Science Foundation (ESF); Global Canopy Programme; Czech Science foundation GACR grant; European Social Fund (ESF); Ministry of Education, Youth & Sports - Czech Republic; French National Research Agency (ANR); Research Council of Norway; Grant Agency of the Czech Republi
Arthropod distribution in a tropical rainforest: tackling a four dimensional puzzle
Quantifying the spatio-temporal distribution of arthropods in tropical rainforests represents
a first step towards scrutinizing the global distribution of biodiversity on Earth. To date moststudies have focused on narrow taxonomic groups or lack a design that allows partitioning
of the components of diversity. Here, we consider an exceptionally large dataset (113,952
individuals representing 5,858 species), obtained from the San Lorenzo forest in Panama,
where the phylogenetic breadth of arthropod taxa was surveyed using 14 protocols targeting
the soil, litter, understory, lower and upper canopy habitats, replicated across seasons
in 2003 and 2004. This dataset is used to explore the relative influence of horizontal, vertical
and seasonal drivers of arthropod distribution in this forest. We considered arthropod abundance,
observed and estimated species richness, additive decomposition of species richness,
multiplicative partitioning of species diversity, variation in species composition,
species turnover and guild structure as components of diversity. At the scale of our study
(2km of distance, 40m in height and 400 days), the effects related to the vertical and seasonal
dimensions were most important. Most adult arthropods were collected from the soil/
litter or the upper canopy and species richness was highest in the canopy. We compared
the distribution of arthropods and trees within our study system. Effects related to the seasonal
dimension were stronger for arthropods than for trees. We conclude that: (1) models
of beta diversity developed for tropical trees are unlikely to be applicable to tropical arthropods;
(2) it is imperative that estimates of global biodiversity derived from mass collecting of
arthropods in tropical rainforests embrace the strong vertical and seasonal partitioning
observed here; and (3) given the high species turnover observed between seasons, global
climate change may have severe consequences for rainforest arthropods
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