Drosophila melanogaster como modelo de la pérdida sináptica temprana en la enfermedad de Alzheimer

Tesis doctoral inédita leída en la Universidad Autónoma de Madrid. Facultad de Ciencias, Departamento de Biología. Fecha de lectura:19-07-201

Elucidating historical fisheries’ networks in the Iberian Peninsula using stable isotopes

[EN] Processed fish is known to have been systematically traded in Europe since the Early Middle Ages to meet increasing demand of incipient markets and religious precepts, yet specific details regarding the supply of different fish in Southern Europe is often missing. Here, we address this gap through an integrated approach involving bone metrics and stable isotopic analysis of archaeological European hake (Merluccius merluccius, Merluccidae) from the Iberian Peninsula. The results offer new insights regarding historical fisheries production systems, including the regions exploited and commercial routes, whilst also revealing biological differences between archaeological and present-day hake populations in the North-east Atlantic and the Mediterranean Sea. This research highlights the fruitful result of combining ichthyoarchaeological and historical data for tracking down the origin and development of historical fisheries in south-western Europe and their bearing on modern fisheries.SIThis research was mainly supported by the European Commission through the FISHARC-IF 658022 Marie-Curie-Sklodowska-IF fellowship for Career development. Additional support was received by the Spanish Ministry of Economy and Competitiveness [HAR 2014-55722-P (Ictioarqueologia de la Prehistoria cantábrica: Modelos para la caracterización de las primeras pesquerías europeas), HAR 2017-88325-P (Historical archaeobiology of the European hake (Merluccius merluccius, L.1758) in the NE Atlantic: The Iberian evidence (AD 10th–18th)), and the European Molecular Biology Organization (ASTF 354-2016). The research is also under the framework of the the Leidse Universiteit Fonds-Bakels 2021 fund, the PID-118662GB-100 (FISHCIIS - Fishing Isotopes) project from the Ministry of Science and Innovation, and the Research Group of Cultural Landscapes and Heritage of the University of the Basque Country. We finally want to thank two anonymous reviewers for their helpful suggestions that have improved the quality of this paper. This work contributes to the “María de Maeztu” Programme for Units of Excellence of the Spanish Ministry of Science and Innovation (CEX2019-000940-M)

Ancient DNA evidence for the ecological globalization of cod fishing in medieval and post-medieval Europe

Understanding the historical emergence and growth of long-range fisheries can provide fundamental insights into the timing of ecological impacts and the development of coastal communities during the last millennium. Whole-genome sequencing approaches can improve such understanding by determining the origin of archaeological fish specimens that may have been obtained from historic trade or distant water. Here, we used genome-wide data to individually infer the biological source of 37 ancient Atlantic cod specimens (ca 1050-1950 CE) from England and Spain. Our findings provide novel genetic evidence that eleventh- to twelfth-century specimens from London were predominantly obtained from nearby populations, while thirteenth- to fourteenth-century specimens were derived from distant sources. Our results further suggest that Icelandic cod was indeed exported to London earlier than previously reported. Our observations confirm the chronology and geography of the trans-Atlantic cod trade from Newfoundland to Spain starting by the early sixteenth century. Our findings demonstrate the utility of whole-genome sequencing and ancient DNA approaches to describe the globalization of marine fisheries and increase our understanding regarding the extent of the North Atlantic fish trade and long-range fisheries in medieval and early modern time

Estudio del uso de dispositivos absorbentes en pacientes incontinentes institucionalizados aplicando una escala de valoración de dermatitis de pañal por humedad

La incontinencia es un problema con elevada presencia en la población geriátrica. La lesiones cutáneas que se desarrollan con más frecuencia son las causadas por la humedad y el contacto directo de la orina y/o las heces sobre la piel. Nuestro objetivo fue diseñar una escala de valoración de la dermatitis del pañal por humedad para desarrollar un plan de cuidados sobre el uso adecuado de los dispositivos absorbentes en personas incontinentes y el tratamiento de lesiones producidas por la humedad. Metodología del estudio: Para el diseño de la escala visual iconográfica de la dermatitis del pañal por humedad (DPH) se realizó un estudio retrospectivo, observacional descriptivo sobre 140 Historias Clínicas. Para la aplicación de la escala de humedad y su tratamiento sobre ancianos con uso de dispositivos absorbentes se realizó un estudio prospectivo multicéntrico, durante una semana, sobre 134 pacientes institucionalizados. Los dispositivos absorbentes utilizados fueron proporcionados por el equipo investigador por las características del producto y para evitar el sesgo en la investigación. Resultados: El 45% de la población a estudio era mayor de 80 años y el 87% eran mujeres. El 88% tenía incontinencia mixta y más del 50% riesgo medio-alto de desarrollar úlceras por presión. Para 78% de los casos el dispositivo absorbente utilizado no presentó incidencias por uso. Con relación a los factores relacionados con la humedad las lesiones que más mejoraron fueron el eritema y la sequedad. Conclusiones: La utilización de escalas de valoración cutánea adecuadas al uso de dispositivos absorbentes favorece el cuidado de la integridad de la piel en pacientes incontinentes.Incontinence is a problem with strong presence in geriatric population. The most common skin lesions are the ones caused by the moisture and by the direct contact with urine and/or feces. Our goal was to design a diaper rash scale for developing a care guide about the proper use of absorbent dressings within incontinent elderly and about the treatment of wounds caused by moisture. Methodology: A retrospective, observational, descriptive study was conducted to design the iconographic visual scale of the diaper rash caused by moisture. A total of 140 patients were screened in this study. A multicenter, prospective study was conducted to apply the moisture scale within the treatment of elderlies with the use of absorbents dressings. A total of 134 institutionalized patients were screened in this study during a week. The absorbent dressings were provided by the investigation team in order to avoid bias in the investigation. Results: 45% of studied patients was over 80 years old, and 87% was women. 88% experienced mixed incontinence, and more than 50% ran the risk of developing pressure ulcers. The absorbent dressing used did not have any problem by its use in the 78% of the studied cases. Regarding the humidity-related factors, erythema and dryness were the most improved lesions. Conclusion: The use of skin lesion scales based on absorbent dressings stimulates the care of the skin integrity in incontinent elderlies.Enfermerí

Ancient DNA evidence for the ecological globalization of cod fishing in medieval and post-medieval Europe

Understanding the historical emergence and growth of long-range fisheries can provide fundamental insights into the timing of ecological impacts and the development of coastal communities during the last millennium. Whole-genome sequencing approaches can improve such understanding by determining the origin of archaeological fish specimens that may have been obtained from historic trade or distant water. Here, we used genome-wide data to individually infer the biological source of 37 ancient Atlantic cod specimens (ca 1050-1950 CE) from England and Spain. Our findings provide novel genetic evidence that eleventh- to twelfth-century specimens from London were predominantly obtained from nearby populations, while thirteenth- to fourteenth-century specimens were derived from distant sources. Our results further suggest that Icelandic cod was indeed exported to London earlier than previously reported. Our observations confirm the chronology and geography of the trans-Atlantic cod trade from Newfoundland to Spain starting by the early sixteenth century. Our findings demonstrate the utility of whole-genome sequencing and ancient DNA approaches to describe the globalization of marine fisheries and increase our understanding regarding the extent of the North Atlantic fish trade and long-range fisheries in medieval and early modern times

Population-based multicase-control study in common tumors in Spain (MCC-Spain): rationale and study design

Introduction: We present the protocol of a large population-based case-control study of 5 common tumors in Spain (MCC-Spain) that evaluates environmental exposures and genetic factors. Methods: Between 2008-2013, 10,183 persons aged 20-85 years were enrolled in 23 hospitals and primary care centres in 12 Spanish provinces including 1,115 cases of a new diagnosis of prostate cancer, 1,750 of breast cancer, 2,171 of colorectal cancer, 492 of gastro-oesophageal cancer, 554 cases of chronic lymphocytic leukaemia (CLL) and 4,101 population-based controls matched by frequency to cases by age, sex and region of residence. Participation rates ranged from 57% (stomach cancer) to 87% (CLL cases) and from 30% to 77% in controls. Participants completed a face-to-face computerized interview on sociodemographic factors, environmental exposures, occupation, medication, lifestyle, and personal and family medical history. In addition, participants completed a self-administered food-frequency questionnaire and telephone interviews. Blood samples were collected from 76% of participants while saliva samples were collected in CLL cases and participants refusing blood extractions. Clinical information was recorded for cases and paraffin blocks and/or fresh tumor samples are available in most collaborating hospitals. Genotyping was done through an exome array enriched with genetic markers in specific pathways. Multiple analyses are planned to assess the association of environmental, personal and genetic risk factors for each tumor and to identify pleiotropic effects. Discussion: This study, conducted within the Spanish Consortium for Biomedical Research in Epidemiology & Public Health (CIBERESP), is a unique initiative to evaluate etiological factors for common cancers and will promote cancer research and prevention in Spain.The study was partially funded by the “Accion Transversal del Cancer”, approved on the Spanish Ministry Council on the 11th October 2007, by the Instituto de Salud Carlos III-FEDER (PI08/1770, PI08/0533, PI08/1359, PS09/00773, PS09/01286, PS09/01903, PS09/02078, PS09/01662, PI11/01403, PI11/01889, PI11/00226, PI11/01810, PI11/02213, PI12/00488, PI12/00265, PI12/01270, PI12/00715, PI12/00150), by the Fundación Marqués de Valdecilla (API 10/09), by the ICGC International Cancer Genome Consortium CLL, by the Junta de Castilla y León (LE22A10-2), by the Consejería de Salud of the Junta de Andalucía (PI-0571), by the Conselleria de Sanitat of the Generalitat Valenciana (AP 061/10), by the Recercaixa (2010ACUP 00310), by the Regional Government of the Basque Country by European Commission grants FOOD-CT- 2006-036224-HIWATE, by the Spanish Association Against Cancer (AECC) Scientific Foundation, by the The Catalan Government DURSI grant 2009SGR1489

A randomized, double-blind study on the efficacy of oral domperidone versus placebo for reducing SARS-CoV-2 viral load in mild-to-moderate COVID-19 patients in primary health care

15 p.-3 fig.-3 tab.Introduction:The clinical effect of domperidone against COVID-19 has been investigated in a double-blind phase III clinical trial (EudraCT number 2021-001228-17). Domperidone has shown in vitro antiviral activity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and potential immudolatory properties through the stimulation of prolactin secretion.Patients and methods:The efficacy of oral domperidone plus standard of care (SOC; n = 87) versus placebo plus SOC (n = 86) was evaluated in a 28-day randomized double-blind multicentre study in primary health care centres. A total of 173 outpatients with mild-to-moderate COVID-19 were included. Three daily doses of 10 mg (30 mg/day) of domperidone or placebo were administered for 7 days. Reduction of viral load on day 4 was the primary efficay endpoint. It was estimated in saliva samples by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), as the cycle thresholds detected ORF1ab, N Protein and S Protein genes.Results:A significant reduction in the viral load was observed (p < 0.001) from baseline to days 4, 7 and 14 of the three genes studied with non-significant differences between domperidone and placebo groups. Twenty-three patients (13.3%) experienced adverse events, 14 patients in the domperidone group (16.1%) and 9 patients in the placebo group (10.5%). No patients needed to be hospitalized.Conclusion: Results do not prove the use of domperidone as antiviral in patients with COVID-19.This research was funded by CSIC (grant no. PIE 201980E024) and by the European Commission: NextGeneration EU (Regulation EU 2020/2094) through CSIC’s Global Health Platform (PTI Salud Global). The study sponsor was Agencia Estatal Consejo Superior de Investigaciones Científicas, M.P. (CSIC), Madrid, Spain. The sponsor was involved in the design, data interpretation, manuscript review and the decision to submit the article for publication.Peer reviewe

Crítica, periodismo y divulgación musical en espacios digitales

Mediante el presente proyecto se ha ofrecido formación en recursos y prácticas de crítica y periodismo musical, profundizando en la importancia de la divulgación en espacios digitales. Se trata de la continuación del anterior proyecto de innovación no 21 "Herramientas de comunicación y divulgación musical orientadas a la transferencia: entornos digitales 2.0". El nuevo proyecto se ha centrado en los ámbitos de la crítica y el periodismo, concretamente en la actualidad de la prensa escrita y la radio, así como ha tenido en cuenta el uso extendido de redes sociales y plataformas online en tareas de divulgación de contenidos. Dicha formación ha querido dar respuesta a la necesidad de que el profesorado y el alumnado en Musicología actualice y refuerce su conocimiento sobre los códigos, recursos, y entornos en los que se desarrollan estos campos, ya que se trata actualmente de uno de los ámbitos principales de proyección profesional de los egresados de Musicología, a través de la demanda de publicaciones digitales que requieren especialización en música, , radios - en las cuales la interacción con el usuario de internet es crucial-, e instituciones culturales y musicales que necesitan reseñas, crónicas, y breves escritos enfocados a sus espacios de difusión y promoción en internet. Se trata de un proyecto interfacultativo e interdepartamental ya que ha implicado a profesionales, docentes, investigadores y alumnado del Departamento de Musicología, el ICCMU (ambos de la Facultad de Geografía e Historia), y el Departamento de Periodismo y Nuevos medios (Facultad de Ciencias de la Información)