Features of organ-saving foot amputation associated with ozone therapy, local application of autologous platelet-rich plasma and vacuum sanitization of postoperative wound in patients with ischemic-gangrenous form of diabetic foot syndrome

Improvement of reparative process of the chronic wounds with the insufficiency of peripheral blood suppling in patients suffering from diabetes mellitus is an important issue requiring further investigation. An optimal choice of surgery, effective renewal of blood supply and active stimulation of tissue repair on the cellular level are essential components of success treatment of this problem.Objective of research: From 2017 to 2020, the efficiency of one of the variants of organ-saving surgery performed on 210 patients with an ischemic-gangrenous form of diabetic foot syndrome was studied.Materials and methods. Control group included 104 patients (49.52%), were the treatment carried out in accordance with the standard scheme. The main group (106 patients - 50.47%) in addition to the standard therapy have used regional ozone therapy, vacuum sanitization and local application of autologous platelet-rich plasma (APRP).Results. These proposed measures reduced the period of clinical treatment to 24±1,2 days in the main group compared to 37±2,4 in the control one. 2 years later, patients of the main group confirmed the formation of a foot stump functionally adapted for walking with angular dislocation of fragments of the cuboid and scaphoid bones.Two years after foot amputation due to the ischemic-gangrenous form of diabetic foot syndrome (DFS), the amount of repeated above knee amputations was 1.8 times higher in the control group in comparison with the main group, which is indicative of a reasonable use of the complex of activate a reparative process in the wound.Conclusions: The suggested modification of the organ-saving and partial foot amputation in patients with IV degree of ischemia and DFS is indicative of a possibility to perform such kind of surgery as a variant of choice.The complex of proposed additional measures including regional ozone therapy, vacuum sanitization and local administration of autologous platelet-rich plasma activates a reparative process of a chronic foot wound and promotes an effective treatment of patients with ischemic-gangrenous form of DFS

Laser photoplethysmography in integrated evaluation of collateral circulation of lower extremities

The paper evaluated the diagnostic value of laser photoplethysmography when examining patients with chronic lower limb ischemia. A statistical analysis of the research results was made, and diagrams of relationship between the degrees of ischemia and blood flow are presente

The Global Alliance for Infections in Surgery: Defining a Model for Antimicrobial Stewardship-Results From an International Cross-Sectional Survey

BACKGROUND: Antimicrobial Stewardship Programs (ASPs) have been promoted to optimize antimicrobial usage and patient outcomes, and to reduce the emergence of antimicrobial-resistant organisms. However, the best strategies for an ASP are not definitively established and are likely to vary based on local culture, policy, and routine clinical practice, and probably limited resources in middle-income countries. The aim of this study is to evaluate structures and resources of antimicrobial stewardship teams (ASTs) in surgical departments from different regions of the world. METHODS: A cross-sectional web-based survey was conducted in 2016 on 173 physicians who participated in the AGORA (Antimicrobials: A Global Alliance for Optimizing their Rational Use in Intra-Abdominal Infections) project and on 658 international experts in the fields of ASPs, infection control, and infections in surgery. RESULTS: The response rate was 19.4%. One hundred fifty-six (98.7%) participants stated their hospital had a multidisciplinary AST. The median number of physicians working inside the team was five [interquartile range 4-6]. An infectious disease specialist, a microbiologist and an infection control specialist were, respectively, present in 80.1, 76.3, and 67.9% of the ASTs. A surgeon was a component in 59.0% of cases and was significantly more likely to be present in university hospitals (89.5%, p \u3c 0.05) compared to community teaching (83.3%) and community hospitals (66.7%). Protocols for pre-operative prophylaxis and for antimicrobial treatment of surgical infections were respectively implemented in 96.2 and 82.3% of the hospitals. The majority of the surgical departments implemented both persuasive and restrictive interventions (72.8%). The most common types of interventions in surgical departments were dissemination of educational materials (62.5%), expert approval (61.0%), audit and feedback (55.1%), educational outreach (53.7%), and compulsory order forms (51.5%). CONCLUSION: The survey showed a heterogeneous organization of ASPs worldwide, demonstrating the necessity of a multidisciplinary and collaborative approach in the battle against antimicrobial resistance in surgical infections, and the importance of educational efforts towards this goal

Intercultural Communication Skills as an International Tool for the Development of English-Language Communicative Competencies

Мета цього експериментального дослідження полягала в тому, щоб з'ясувати, чи можна використовувати навички міжкультурного спілкування як інструмент розвитку комунікативних англомовних компетенцій. Для цього використовувався досвід трьох країн: Нідерландів, Сінгапуру та Бельгії. Населення цих країн має високий рівень володіння англійською як іноземною. У ході дослідження було проведено педагогічний експеримент. Проведено опитування 137 учасників експерименту. Результати обробляли математичними методами дисперсійного аналізу, коефіцієнту Коена та критерію Пірсона. Також використовувалося програмне забезпечення статистики. Виявлено, що використання таких методів навчання англійської мови як перегляд англомовних фільмів, читання англомовних художніх творів мовою оригіналу сприяє розвитку навичок міжкультурної комунікації. Ефективна участь у живому спілкуванні студентів з англомовними іноземними однокласниками та віртуальний обмін. Однак найкращих результатів можна досягти, послідовно застосовуючи ці три методи. Дослідження показало, що використовувані методи навчання, які сприяли розвитку навичок міжкультурного спілкування, також позитивно вплинули на розвиток англомовних комунікативних компетенцій. Встановлений зв'язок дає підстави стверджувати, що навички міжкультурної комунікації можна використовувати як інструмент розвитку англомовних комунікативних компетенцій. Подальші дослідження мають бути зосереджені на пошуку способів оптимізації навчальних програм, пошуку ефективних методів та орієнтації учасників на розвиток навичок міжкультурного спілкування.Цель этого экспериментального исследования состояла в том, чтобы выяснить, можно ли использовать навыки межкультурного общения в качестве инструмента для развития коммуникативных англоязычных компетенций. Для этого использовался опыт трех стран: Нидерландов, Сингапура и Бельгии. Население этих стран имеет высокий уровень владения английским языком как иностранным. В ходе исследования был проведен педагогический эксперимент. Был проведен опрос 137 участников эксперимента. Результаты обрабатывали математическими методами дисперсионного анализа, коэффициента Коэна и критерия Пирсона. Также использовалось программное обеспечение для статистики. Выявлено, что использование таких методов обучения английскому языку, как просмотр англоязычных фильмов, чтение англоязычных художественных произведений на языке оригинала, способствует развитию навыков межкультурной коммуникации. Эффективны участие в живом общении студентов с англоязычными иностранными студентами и виртуальный обмен. Однако наилучших результатов можно добиться, последовательно применяя эти три метода. Исследование показало, что используемые методы обучения, которые способствовали развитию навыков межкультурного общения, также оказали положительное влияние на развитие англоязычных коммуникативных компетенций. Установленная связь дает основание утверждать, что навыки межкультурной коммуникации могут быть использованы как инструмент развития англоязычных коммуникативных компетенций. Дальнейшие исследования должны быть сосредоточены на поиске способов оптимизации учебных программ, поиске эффективных методов и ориентации участников на развитие навыков межкультурного общения.The aim of this experimental study was to find out whether intercultural communication skills can be used as a tool to develop communicative English-speaking competencies. The experience of three countries: the Netherlands, Singapore, and Belgium was used for this purpose. The population of these countries has a high level of English as a foreign language. The pedagogical experiment was conducted in the course of research. A survey of the 137 participants in the experiment was conducted. The results were processed by mathematical methods of analysis of variance, Cohen’s coefficient and Pearson’s chi-squared test. Statistics software was also used. It was found that the use of such English teaching methods as watching English-language films, and reading English-language art works in the original language promotes the development of intercultural communication skills. Participation in live communication between students and English-speaking foreign classmates and virtual exchange are effective. However, the best results can be achieved by applying these three methods in sequence. The study showed that the teaching methods used, which contributed to the development of intercultural communication skills, also had a positive impact on the development of English-language communicative competencies. The established connection gives grounds to claim that the intercultural communication skills can be used as a tool for the development of English-language communicative competencies. Further research should focus on finding ways to optimize curricula, finding effective methods and focusing participants on the development of intercultural communication skills

Device to determine the level of peripheral blood circulation and saturation

The paper evaluated the diagnostic value of laser photoplethysmography when examining patients with chronic lower limb ischemia. A statistical analysis of the research results was made, and diagrams of relationship between the degrees of ischemia and blood flow are presented. Development of the device to determine the level of peripheral blood circulation and saturation was presented. Also additional accessories in the form of optical fibers for different applications were suggested

Оцінка забруднення підземних вод іонами важких металів при поводженні з пально-мастильними матеріалами

Козловська Т. Ф. Оцінка забруднення підземних вод іонами важких металів при поводженні з пально-мастильними матеріалами / Т. Ф. Козловська , В. М. Сиволожська, В. І. Панченко / Авіація, промисловість, суспільство : матеріали ІІІ Міжнар. наук.-практ. конф. (м. Кременчук, 12 трав. 2022 р.) / МВС України, Харків. нац. ун-т внутр. справ, Кременчуц. льотний коледж, Наук. парк «Наука та безпека». – Харків : ХНУВС, 2022. – С. 151-154.Зазначено, що розташування джерела забруднення в екологічному відношенні становить серйозну загрозу для оточуючого середовища, особливо для підземних вод, а відсутність надійного водоупору і високі ухили сприяють розтіканню забрудненого потоку до селітебної зони. Отмечено, что расположение источника загрязнения в экологическом отношении представляет серьезную угрозу окружающей среде, особенно для подземных вод, а отсутствие надежного водоупора и высокие уклоны способствуют растеканию загрязненного потока в селитебную зону. It is noted that the location of the source of pollution in ecological terms poses a serious threat to the environment, especially for groundwater, and the lack of a reliable aquiclude and high slopes contribute to the spread of the polluted stream into the residential area

High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model

Copyright: Copyright 2014 Elsevier B.V., All rights reserved.Background: The combination of virotherapy and chemotherapy may enable efficient tumor regression that would be unachievable using either therapy alone. In this study, we investigated the efficiency of transgene delivery and the cytotoxic effects of alphaviral vector in combination with 5-fluorouracil (5-FU) in a mouse mammary tumor model (4 T1).Methods: Replication-deficient Semliki Forest virus (SFV) vectors carrying genes encoding fluorescent proteins were used to infect 4 T1 cell cultures treated with different doses of 5-FU. The efficiency of infection was monitored via fluorescence microscopy and quantified by fluorometry. The cytotoxicity of the combined treatment with 5-FU and alphaviral vector was measured using an MTT-based cell viability assay. In vivo experiments were performed in a subcutaneous 4 T1 mouse mammary tumor model with different 5-FU doses and an SFV vector encoding firefly luciferase.Results: Infection of 4 T1 cells with SFV prior to 5-FU treatment did not produce a synergistic anti-proliferative effect. An alternative treatment strategy, in which 5-FU was used prior to virus infection, strongly inhibited SFV expression. Nevertheless, in vivo experiments showed a significant enhancement in SFV-driven transgene (luciferase) expression upon intratumoral and intraperitoneal vector administration in 4 T1 tumor-bearing mice pretreated with 5-FU: here, we observed a positive correlation between 5-FU dose and the level of luciferase expression.Conclusions: Although 5-FU inhibited SFV-mediated transgene expression in 4 T1 cells in vitro, application of the drug in a mouse model revealed a significant enhancement of intratumoral transgene synthesis compared with 5-FU untreated mice. These results may have implications for efficient transgene delivery and the development of potent cancer treatment strategies using alphaviral vectors and 5-FU.publishersversionPeer reviewe

VLPs and particle strategies for cancer vaccines

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The Global Alliance for Infections in Surgery : defining a model for antimicrobial stewardship-results from an international cross-sectional survey

Background: Antimicrobial Stewardship Programs (ASPs) have been promoted to optimize antimicrobial usage and patient outcomes, and to reduce the emergence of antimicrobial-resistant organisms. However, the best strategies for an ASP are not definitively established and are likely to vary based on local culture, policy, and routine clinical practice, and probably limited resources in middle-income countries. The aim of this study is to evaluate structures and resources of antimicrobial stewardship teams (ASTs) in surgical departments from different regions of the world. Methods: A cross-sectional web-based survey was conducted in 2016 on 173 physicians who participated in the AGORA (Antimicrobials: A Global Alliance for Optimizing their Rational Use in Intra-Abdominal Infections) project and on 658 international experts in the fields of ASPs, infection control, and infections in surgery. Results: The response rate was 19.4%. One hundred fifty-six (98.7%) participants stated their hospital had a multidisciplinary AST. The median number of physicians working inside the team was five [interquartile range 4-6]. An infectious disease specialist, a microbiologist and an infection control specialist were, respectively, present in 80.1, 76.3, and 67.9% of the ASTs. A surgeon was a component in 59.0% of cases and was significantly more likely to be present in university hospitals (89.5%, p <0.05) compared to community teaching (83.3%) and community hospitals (66.7%). Protocols for pre-operative prophylaxis and for antimicrobial treatment of surgical infections were respectively implemented in 96.2 and 82.3% of the hospitals. The majority of the surgical departments implemented both persuasive and restrictive interventions (72.8%). The most common types of interventions in surgical departments were dissemination of educational materials (62.5%), expert approval (61.0%), audit and feedback (55.1%), educational outreach (53.7%), and compulsory order forms (51.5%). Conclusion: The survey showed a heterogeneous organization of ASPs worldwide, demonstrating the necessity of a multidisciplinary and collaborative approach in the battle against antimicrobial resistance in surgical infections, and the importance of educational efforts towards this goal.Peer reviewe

СПОСІБ ПЕРЕДОПЕРАЦІЙНОЇ ПІДГОТОВКИ ХВОРИХ НА ХРОНІЧНІ ТРІЩИНИ ВІДХІДНИКА

The use of intratissue electrophoresis with a dioksyzol solution as a preoperative preparation of patients with chronic anal fissures of the void causes reduction of the manifestation of hemodynamic disorder, acute inflammatory responses in tissues anal fissure and encourages the development of young granulation tissue in the area of the wound edges and the edge of the resection, contributes to the reduction of the intensity of the pain syndrome, reduces expression of inflammation, stimulates reparative processes in the area of the crack, accelerates epithelization and the healing of surgical wounds, quickly removes spasm of the sphincter and anal pain, tearing main pathogenetic mechanism of the disease.Применение внутритканевого электрофореза с раствором диоксизоля в качестве предоперационной подготовки больных хроническими анальными трещинами приводит к уменьшению проявления гемодинамического расстройства, острых воспалительных реакций в тканях анальной трещины и стимулирует развитие молодой грануляционной ткани в области краев раны и края резекции, способствует уменьшению интенсивности болевого синдрома, уменьшает проявление воспалительного процесса, стимулирует репаративные процессы в области трещины, ускоряет эпителизацию и срок заживления послеоперационной раны, быстро устраняет спазм анального сфинктера и болевой синдром, разрывая основной патогенетический механизм развития данного заболевания.Застосування внутрішньотканинного електрофорезу з розчином діоксизолю в якості передопераційної підготовки хворих на хронічні тріщини відхідника зумовлює зменшення прояву гемодинамічного розладу, гострих запальних реакцій у тканинах анальної тріщини і стимулює розвиток молодої грануляційної тканини в ділянці країв рани і краю резекції, сприяє зменшенню інтенсивності больового синдрому, стимулює репаративні процеси в ділянці тріщини, пришвидшує епітелізацію і термін загоєння післяопераційної рани, швидко усуває спазм анального сфінктера та больовий синдром, розриваючи основний патогенетичний механізм розвитку досліджуваного захворювання