Evaluation of Sesquiterpenes from Ferula assa-foetida on inflamatory parameters and study of biding modes using computational methods

introduction: Ferula assafoetida is a source of sesquiterpenes [1]. According to an investigation, phenolic compounds at physiological concentration can inhibit inflammatory enzymes [2]. These enzymes digest the extracellular matrix and provide the conditions for activation and migration and proliferation of endothelial cells. Reported studies on medicinal plants for their inhibitory effect on MMP are very limited. Methods and Results: Acetone extract of plant was prepared and Sesquiterpenes were purified using HPLC preparative analyses and their structures were elucidated. After culturing the cell at confluence, cells were isolated and the supernatant was removed. The pure substances were applied on cell lines U87MG and Wehi activities. Besides the structure has been docked in the active site of metalloproteinase, and significant interactions were determined.Subsequently, ligand-protein complexes were subjected to molecular dynamics simulation in water and thermodynamic properties were calculated. In the phytochemistry field galbanic acid, mogoltadone, kellerin, polyanthin and polyanthininwere produced from F. assafoetida. The results of celluar toxicity study shows that IC50 of Galbanic acid, Mogoltadone and Polyanthin in Wehi cell line were 925.2703, 721.86, and 680.3 µg/ml in U87MG cell line were 952.193, 752.352, 678.742. Galbanic acid, mogoltadone, kellerin, polyanthin and polyanthininwere solated from F. assafoetida. The results of celluar toxicity study show that IC50 of Galbanic acid, Mogoltadone and Polyanthin in Wehi cell line were 925.2703, 721.86, and 680.3 µg/ml in U87MG cell line were 952.193, 752.352, 678.742 Conclusion: Investigation revealed that the coumarins have inhibitory effects on the content and activity of MMP 2.9 and showed anti-angiogenetic effect. So, they can be potentially effective in the treatment of cancer. Interactive and competitive binding between MMP-9 and Galbanic acid were studied with FT-IR, UV-Vis and fluorescence methods and MMP-9 structure was changed in these interactions

Short and long term complications after pediatric liver transplantation: A review and literature

Background Pediatric liver transplant (PLT) is a complex surgical procedure. Pediatric liver transplantation has evolved over the last two decades into an effective and widely accepted therapy for infants and children. We aimed to review the short and long term complications after oediatric liver transplantation. Materials and Methods The literature research was conducted in EMBASE, PubMed, Scopus, ISI Web of Science, Cochrane Library, and Google Scholar; were searched from January 1984 to January 2016. The following keywords were used: Liver transplantation, Pediatric Liver transplantation, Risk factors, Complication, and Mortality. Results The main complications after pediatric liver transplantation were: infections (51.4) and surgical complications including (biliary complications 41.2%, and postoperative bleeding 27%). In general, vascular complications were observed in 35% of studied children. Conclusion Infections and biliary complications were the most common outcome occurring in children after LT. Advances in post-transplant care and monitoring of the recipients, technical refinements enable the better results

Short and long term complications after pediatric liver transplantation: A review and literature

Background Pediatric liver transplant (PLT) is a complex surgical procedure. Pediatric liver transplantation has evolved over the last two decades into an effective and widely accepted therapy for infants and children. We aimed to review the short and long term complications after oediatric liver transplantation. Materials and Methods The literature research was conducted in EMBASE, PubMed, Scopus, ISI Web of Science, Cochrane Library, and Google Scholar; were searched from January 1984 to January 2016. The following keywords were used: Liver transplantation, Pediatric Liver transplantation, Risk factors, Complication, and Mortality. Results The main complications after pediatric liver transplantation were: infections (51.4) and surgical complications including (biliary complications 41.2%, and postoperative bleeding 27%). In general, vascular complications were observed in 35% of studied children. Conclusion Infections and biliary complications were the most common outcome occurring in children after LT. Advances in post-transplant care and monitoring of the recipients, technical refinements enable the better results

MUSiC : a model-unspecific search for new physics in proton-proton collisions at root s=13TeV

Results of the Model Unspecific Search in CMS (MUSiC), using proton-proton collision data recorded at the LHC at a centre-of-mass energy of 13 TeV, corresponding to an integrated luminosity of 35.9 fb(-1), are presented. The MUSiC analysis searches for anomalies that could be signatures of physics beyond the standard model. The analysis is based on the comparison of observed data with the standard model prediction, as determined from simulation, in several hundred final states and multiple kinematic distributions. Events containing at least one electron or muon are classified based on their final state topology, and an automated search algorithm surveys the observed data for deviations from the prediction. The sensitivity of the search is validated using multiple methods. No significant deviations from the predictions have been observed. For a wide range of final state topologies, agreement is found between the data and the standard model simulation. This analysis complements dedicated search analyses by significantly expanding the range of final states covered using a model independent approach with the largest data set to date to probe phase space regions beyond the reach of previous general searches.Peer reviewe

Measurement of prompt open-charm production cross sections in proton-proton collisions at root s=13 TeV

The production cross sections for prompt open-charm mesons in proton-proton collisions at a center-of-mass energy of 13TeV are reported. The measurement is performed using a data sample collected by the CMS experiment corresponding to an integrated luminosity of 29 nb(-1). The differential production cross sections of the D*(+/-), D-+/-, and D-0 ((D) over bar (0)) mesons are presented in ranges of transverse momentum and pseudorapidity 4 < p(T) < 100 GeV and vertical bar eta vertical bar < 2.1, respectively. The results are compared to several theoretical calculations and to previous measurements.Peer reviewe

Search for new particles in events with energetic jets and large missing transverse momentum in proton-proton collisions at root s=13 TeV

A search is presented for new particles produced at the LHC in proton-proton collisions at root s = 13 TeV, using events with energetic jets and large missing transverse momentum. The analysis is based on a data sample corresponding to an integrated luminosity of 101 fb(-1), collected in 2017-2018 with the CMS detector. Machine learning techniques are used to define separate categories for events with narrow jets from initial-state radiation and events with large-radius jets consistent with a hadronic decay of a W or Z boson. A statistical combination is made with an earlier search based on a data sample of 36 fb(-1), collected in 2016. No significant excess of events is observed with respect to the standard model background expectation determined from control samples in data. The results are interpreted in terms of limits on the branching fraction of an invisible decay of the Higgs boson, as well as constraints on simplified models of dark matter, on first-generation scalar leptoquarks decaying to quarks and neutrinos, and on models with large extra dimensions. Several of the new limits, specifically for spin-1 dark matter mediators, pseudoscalar mediators, colored mediators, and leptoquarks, are the most restrictive to date.Peer reviewe

Combined searches for the production of supersymmetric top quark partners in proton-proton collisions at root s=13 TeV

A combination of searches for top squark pair production using proton-proton collision data at a center-of-mass energy of 13 TeV at the CERN LHC, corresponding to an integrated luminosity of 137 fb(-1) collected by the CMS experiment, is presented. Signatures with at least 2 jets and large missing transverse momentum are categorized into events with 0, 1, or 2 leptons. New results for regions of parameter space where the kinematical properties of top squark pair production and top quark pair production are very similar are presented. Depending on themodel, the combined result excludes a top squarkmass up to 1325 GeV for amassless neutralino, and a neutralinomass up to 700 GeV for a top squarkmass of 1150 GeV. Top squarks with masses from 145 to 295 GeV, for neutralino masses from 0 to 100 GeV, with a mass difference between the top squark and the neutralino in a window of 30 GeV around the mass of the top quark, are excluded for the first time with CMS data. The results of theses searches are also interpreted in an alternative signal model of dark matter production via a spin-0 mediator in association with a top quark pair. Upper limits are set on the cross section for mediator particle masses of up to 420 GeV

Development and validation of HERWIG 7 tunes from CMS underlying-event measurements

This paper presents new sets of parameters (“tunes”) for the underlying-event model of the HERWIG7 event generator. These parameters control the description of multiple-parton interactions (MPI) and colour reconnection in HERWIG7, and are obtained from a fit to minimum-bias data collected by the CMS experiment at s=0.9, 7, and 13Te. The tunes are based on the NNPDF 3.1 next-to-next-to-leading-order parton distribution function (PDF) set for the parton shower, and either a leading-order or next-to-next-to-leading-order PDF set for the simulation of MPI and the beam remnants. Predictions utilizing the tunes are produced for event shape observables in electron-positron collisions, and for minimum-bias, inclusive jet, top quark pair, and Z and W boson events in proton-proton collisions, and are compared with data. Each of the new tunes describes the data at a reasonable level, and the tunes using a leading-order PDF for the simulation of MPI provide the best description of the dat