VIPACFood: valorização de subprodutos alimentares para desenvolvimento de filmes e revestimentos que visem aumentar a vida útil de alimentos

O projeto VIPACFood é o acrónimo de “Valorization of Industrial fruits by Products and algae biomass waste: Development of Active Coatings to extend Food shelf life and reduce food losses”. Trata-se de um projeto de 3 anos que teve início no dia 1 de Junho de 2017 e é financiado pelo ARIMNet2. O consórcio do projeto é formado por oito organizações de investigação e governamentais, abrangendo quatro países, Tunísia, Itália, Portugal e Espanha. O coordenador é a Doutora Khaoula Khwaldia (National Institute of Research and Physicochemical Analysis, Tunísia). O projeto visa desenvolver tecnologias pós-colheita seguras e acessíveis que conduzam à redução de perdas, à melhoria da segurança alimentar e prazo de validade, reduzindo a quantidade de embalagem usada para preservar os alimentos, das quais resultam benefícios sociais, ambientais e económicos. O projeto também tem o objetivo de valorizar subprodutos industriais de frutas e resíduos de biomassa de algas, extraindo componentes ativos e funcionais com elevado valor e pela conceção de novos produtos transformados com grande apelo, estabilidade e potencialidade de comercialização que pode ajudar na redução dos subprodutos e resíduos eliminados e terá um impacto positivo na sustentabilidade das indústrias de transformação. Além de aumentar a eficiência económica e aumentar a competitividade dos produtores locais e das pequenas e médias empresas, espera-se que o projeto tenha impacto ambiental e na saúde devido à valorização de subprodutos, à redução significativa dos resíduos alimentares e à melhoria da qualidade dos alimentos e vida útil dos mesmosO presente trabalho insere-se no projeto VIPACFood. Este projeto é financiado pelo ARIMNet2 (Coordination of Agricultural Research in the Mediterranean; 2014-2017), uma ação ERA-NET financiada pela União Europeia (7º Programa Quadro).N/

Effects of oleuropein-rich olive leaf extract on the oxidative stability of refined sunflower oil

The aim of this study is to investigate the ability of oleuropein-rich olive leaf extract (OLE) to improve the quality and oxidative stability of sunflower oil subjected to accelerated thermal oxidation. Oleuropein was the major phenolic compound determined by HPLC-DAD with a content ranging from 20.81 to 32.56 mg·g-1 of dry extract (DE). The evaluation of the in vitro antioxidant capacity of OLE showed good scavenging capacity of the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and hydrogen peroxide (H2O2) (1.01 and 0.96 mmol Trolox equivalents (ET·g-1 DE, respectively). The enrichment of sunflower oil with 0.1, 0.25 and 0.5% OLE (w/v) significantly inhibited thermal-induced peroxidation in a dose-dependent fashion. 0.25% OLE was the most effective concentration and showed a significant reduction in peroxide value and conjugated dienes by 61.4 and 17.4%. These results indicate that OLE can be considered a good natural alternative for extending the shelf-life of polyunsaturated vegetable oils

Citrus By-Products: Valuable Source of Bioactive Compounds for Food Applications

This article belongs to the Special Issue Antioxidant Compounds Recovered from Food Wastes.ReviewCitrus production produces about 15 million tons of by-products/waste worldwide every year. Due to their high content of bioactive compounds, several extraction techniques can be applied to obtain extracts rich in valuable compounds and further application into food applications. Distillation and solvent extraction continues to be the most used and applied extraction techniques, followed by newer techniques such as microwave-assisted extraction and pulsed electric field extraction. Although the composition of these extracts and essential oils directly depends on the edaphoclimatic conditions to which the fruit/plant was exposed, the main active compounds are D-limonene, carotenoids, and carbohydrates. Pectin, one of the most abundant carbohydrates present in Citrus peels, can be used as a biodegradable polymer to develop new food packaging, and the extracted bioactive compounds can be easily added directly or indirectly to foods to increase their shelf-life. One of the applications is their incorporation in active food packaging for microbiological and/or oxidation inhibition, prolonging foods’ shelf-life and, consequently, contributing to reducing food spoilage. This review highlights some of the most used and effective extraction techniques and the application of the obtained essential oils and extracts directly or indirectly (through active packaging) to foods.This paper was carried out under the VIPACFood project, funded by ARIMNet2 (Coordina tion of Agricultural Research in the Mediterranean; 2014–2017), an ERA-NET Action financed by the European Union under the Seventh Framework Programme, and by the Programa de Cooperación Interreg V-A España–Portugal (POCTEP) 2014–2020 (project 0377_IBERPHENOL_6_E). Mariana A. Andrade is grateful for her research grant (SFRH/BD/138730/2018) funded by the Foundation for Science and Technology (FCT). Cássia H. Barbosa is grateful for her research grant in the frame of the VIPACFood project (ARIMNET2/0003/2016) and the Foundation for Science and Technology (FCT), Portugal for the Ph.D. Grant 2021.08154.BD. This work was financially supported by the Mechanical Engineering and Resource Sustainability Center—MEtRICs, which is financed by national funds from the FCT/MCTES (UIDB/50006/2020, UIDB/04077/2020 and UIDP/04077/2020).info:eu-repo/semantics/publishedVersio

Preparation, Characterization and Effectiveness to Delay Lipid Oxidation in Almonds and Beef Meat

Funding Information: This study was carried out under the VIPACFood project, funded by ARIMNet2 (Coordination of Agricultural Research in the Mediterranean; 2014–2017), an ERA-NET Action financed by the European Union under the Seventh Framework Programme, and by the Programa de Cooperación Interreg V-A España–Portugal (POCTEP) 2014–2020 (project 0377_IBERPHENOL_6_E). Cássia H. Barbosa is grateful for her research grant in the frame of the VIPACFood project (ARIMNET2/0003/2016) and the Foundation for Science and Technology (FCT), Portugal, for the Ph.D. grant 2021.08154.BD. This research was also funded by PT national funds (FCT/MCTES, Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through grants UIDB/50006/2020 and UIDB/00211/2020. L. Barbosa-Pereira is grateful to the Spanish Ministry of Science, Innovation and Universities for her “Juan de la Cierva-Incorporación” grant (Agreement No. IJCI-2017-31665). Publisher Copyright: © 2023 by the authors.Low-density polyethylene-based packaging with 4% lemon extract (LDPE/4LE) and two polylactic-based (PLA) packaging materials with 4% and 6% lemon extract (PLA/PEG/4LE and PLA/6LE) were produced. O2 and water permeability tests were performed, the total and individual phenolic compounds content were measured, and the films’ antioxidant activities were determined. The films’ ability to delay lipid oxidation was tested in two model foods: almonds, packaged with LDPE/4LE, PLA/4LE and PLA/6LE for a maximum period of 60 days at 40 °C (accelerated assay); and beef meat, packaged with the PLA/6LE for a maximum period of 11 days at 4 °C. The LE improved the WVP in all of the active films by 33%, 20% and 60% for the LDPE/4LE, PLA/4LE and PLA/6LE films, respectively. At the end of 10 days, the migration of phenolic compounds through the PLA films was measured to be 142.27 and 114.9 μg/dm2 for the PLA/4LE and PLA/6LE films, respectively, and was significantly higher than phenolic compounds migration measured for the LDPE/4LE (15.97 μg/dm2). Naringenin, apigenin, ferulic acid, eriocitrin, hesperidin and 4-hydroxybenzoic acid were the main identified compounds in the PLA, but only 4-hydroxybenzoic acid, naringenin and p-coumaric acid were identified in the LDPE films. Regarding the films’ ability to delay lipid oxidation, LDPE/4LE presented the best results, showing a capacity to delay lipid oxidation in almonds for 30 days. When applied to raw beef meat, the PLA/6LE packaging was able to significantly inhibit lipid oxidation for 6 days, and successfully inhibited total microorganisms’ growth until the 8th day of storage.publishersversionpublishe

Physical effects upon whey protein aggregation for nano-coating production

Production of edible nanostructures constitutes a major challenge in food nanotechnology, and has attracted a great deal of interest from several research fields — including (but not limited to) food packaging. Furthermore, whey proteins are increasingly used as nutritional and functional ingredients owing to their important biological, physical and chemical functionalities. Besides their technological and functional characteristics, whey proteins are generally recognized as safe (GRAS). Denaturation and aggregation kinetics behavior of such proteins are of particular relevance toward manufacture of novel nanostructures possessing a number of potential uses. When these processes are properly engineered and controlled, whey proteins may form nanostructures useful as carriers of bioactive compounds (e.g. antimicrobials, antioxidants and nutraceuticals). This review discusses the latest advances in nano-scale phenomena involved in protein thermal aggregation aiming at formation of bio-based nano-coating networks. The extent of aggregation is dependent upon a balance between molecular interactions and environmental factors; therefore, the impact of these conditions is addressed in a critical manner. A particular emphasis is given to the effect of temperature as long as being one of the most critical variables. The application of moderate electric fields (MEF), an emergent approach, as such or combined with conventional heating is considered as it may inhibit/prevent excessive denaturation and aggregation of whey proteins — thus opening new perspectives for development of innovative protein nanostructures (i.e. nano-coatings). A better understanding of the mechanism(s) involved in whey protein denaturation and aggregation is crucial as it conveys information relevant to select methods for manipulating interactions between molecules, and thus control their functional properties in tailor-made applications in the food industry.Oscar L. Ramos and Ricardo N. Pereira gratefully acknowledge Post-Doctoral grants (SFRH/BPD/80766/2011 and SFRH/BPD/81887/2011, respectively) by Fundacao para a Ciencia e Tecnologia (FCT, Portugal). All authors thank the FCT Strategic Project PEst-OE/EQB/LA0023/2013 and Project "BioEnv - Biotechnology and Bioengineering for a sustainable world", REF. NORTE-07-0124-FEDER-000048, co-funded by Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER

Water-repellent cellulose fiber networks with multifunctional properties.

We demonstrate a simple but highly efficient technique to introduce multifunctional properties to cellulose fiber networks by wetting them with ethyl-cyanoacrylate monomer solutions containing various suspended organic submicrometer particles or inorganic nanoparticles. Solutions can be applied on cellulosic surfaces by simple solution casting techniques or by dip coating, both being suitable for large area applications. Immediately after solvent evaporation, ethyl-cyanoacrylate starts cross-linking around cellulose fibers under ambient conditions because of naturally occurring surface hydroxyl groups and adsorbed moisture, encapsulating them with a hydrophobic polymer shell. Furthermore, by dispersing various functional particles in the monomer solutions, hydrophobic ethyl-cyanoacrylate nanocomposites with desired functionalities can be formed around the cellulose fibers. To exhibit the versatility of the method, cellulose sheets were functionalized with different ethyl-cyanoacrylate nanocomposite shells..

Active Whey Protein Edible Films and Coatings Incorporating Lactobacillus buchneri for Penicillium nordicum Control in Cheese

Fungal contamination of food is responsible for health issues and food waste. In this work, the incorporation of a lactic acid bacteria (LAB) with antifungal properties (Lactobacillus buchneri UTAD104) into whey protein-based films and coatings was tested for the control of an ochratoxigenic fungi (Penicillium nordicum) in a cheese matrix. The incorporation of L. buchneri cells resulted in thicker films with less luminosity than control films and colour alteration. Nevertheless, cells inclusion did not alter moisture content, water vapour permeability, mechanical properties, hydrophobicity and chemical structure of the films. Whey protein films were able to maintain the viability of L. buchneri UTAD104 cells in 105 CFU/mL after 30 days of storage at 25 \textdegreeC. When applied in cheese, films and coatings containing L. buchneri cells prevented fungal contamination for at least 30 days, while control cheeses with films and coatings either without LAB or with Lactobacillus casei UM3 (a strain without antifungal ability) showed fungal contamination during that period. Ochratoxin A was not found in cheeses treated with films and coatings containing L. buchneri UTAD104. Results showed that the inclusion of a LAB with antifungal properties in edible films and coatings can help to reduce or eliminate P. nordicum contamination in cheeses.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Ana Guimarães received support through grant SFRH/BD/103245/2014 from the Portuguese FCT.info:eu-repo/semantics/publishedVersio

Mise au point, caractérisation et application d'un emballage biodégradable complexe à base de fibres cellulosiques (papier) et de protéines animales (caséinate de sodium)

Des dispersions de couchage composées de substances naturelles (mica, cire de carnauba, glycérol et caséinate de sodium) ont été préparées et ensuite déposées sur la surface poreuse du papier. L'influence de chaque constituant des dispersions de couchage sur les propriétés barrières à l'oxygène et à la vapeur d'eau et les propriétés mécaniques a été étudiée. L'utilisation de la méthode des surfaces de réponse permet de quantifier l'influence et les interactions des facteurs (concentration en mica, concentration en cire, concentration en glycérol et concentration en caséinate de sodium). La modélisation par méthode empirique des propriétés barrières à la vapeur d'eau et à l'oxygène et les propriétés mécaniques des papiers, utilise une matrice de Doehlert et conduit à l'optimisation des facteurs étudiés. Nos résultats ont montré que l'enduction à base de caséinate de sodium améliore considérablement les propriétés barrières à l'oxygène du papier. La perméabilité à l'oxygène des papiers couchés (10 g/m2) est 13 à 90 fois inférieure à celle des papiers non couchés (0 g/m1. En effet, l'enduit protéique rend la surface du papier plus lisse et plus homogène que la surface rugueuse du papier non enduit. L'enduit déposé sur la surface du papier améliore également la résistance mécanique et l'élongation du papier en remplissant les pores et les espaces interfibrillaires de la structure fibreuse du papier.The combined effects of mica (0-1.2% (w/w)), carnauba wax (0-0.8% (w/w)), glycerol (0-6% (w/w)) and sodium caseinate (10-13% (w/w)) concentrations on barrier and mechanical properties of coated paper were studied. ln the first part of this work, the use of Response Surface Methodology led to quantify the effects and the interactions of different variables (mica, carnauba wax, glycerol and sodium caséinate concentrations). An empirical model using a Doehlert matrix was developed to account for the overall interactions between parameters and allow selection of the most efficient monitoring parameters on a large scale. Results showed that coating significantly increased oxygen barrier property. Indeed, protein coating increased homogeneity and smoothness of the paper surface by filling the porous structure of the paper. The tensile strength and elongation of coated paper, having a 10 g.m-2 coating weight were greater than that ofuncoated paper, which means that coating increased paper strength and ductility.NANCY/VANDOEUVRE-INPL (545472102) / SudocSudocFranceF