Guest Editorial

This is an editorial which introduces original papers produced on the theme of the supervision of social work practiceThis is an Accepted Manuscript of an article published by Taylor & Francis in Practice: Social Work in Action on September 2015, available online: http://dx.doi.org/10.1080/09503153.2015.1048053This guest editorial introduces the special edition on the supervision of social work practic

Containment - exploring the concept of agency in children’s statutory encounters with social workers

This article examines children’s agency in their interactions with social workers during statutory encounters in a child protection context. It draws from a UK wide ethnographic study. It finds that much of social workers’ responses to children’s agency in this context are best understood as a form of ‘containment’. In doing so, it offers an original and significant contribution to the theoretical understanding of children’s agency, as well as its application in social work practice

Tracking intracellular uptake and localisation of alkyne tagged fatty acids using Raman spectroscopy

Intracellular uptake, distribution and metabolism of lipids is a tightly regulated characteristic in healthy cells. An analytical technique capable of understanding these characteristics with a high level of species specificity in a minimally invasive manner is highly desirable in order to understand better how these become disrupted during disease. In this study, the uptake and distribution of three different alkyne tagged fatty acids in single cells was monitored and compared, highlighting the ability of Raman spectroscopy combined with alkyne tags for better understanding of the fine details with regards to uptake, distribution and metabolism of very chemically specific lipid species. This indicates the promise of using Raman spectroscopy directly with alkyne tagged lipids for cellular studies as opposed to subsequently clicking of a fluorophore onto the alkyne for fluorescence imaging

Public Acceptance of Nature-based Solutions (NbS): a Framework for Successful NbS and its Application in Three European Case Studies

Nature-based solutions (NbS) are increasingly recognized as sustainable approaches to address socio-environmental challenges. Disaster risk reduction (DRR) has benefited by increasingly moving away from purely ‘grey’ infrastructure measures towards NbS, which can better provide cultural, aesthetic, and recreational co-benefits that are highly valued among European citizens. Public acceptance is of particular importance for NbS since they often rely on collaborative implementation, management, and monitoring, as well as protection against competing land uses. Although public engagement is a common goal of NbS projects and found in the IUCN’s core principles of NbS, outreach efforts are rarely based on a sufficient understanding of the (de)motivating factors tied to public perceptions. As a first step, we conducted a systematic literature review to examine how unique NbS characteristics relate to public acceptance through a comparison with grey measures. We identified influential acceptance factors related to individuals, society, and DRR measures. Based on the review, we introduce the PA-NbS framework as a starting point for the systematic consideration, design, and testing of strategies for increasing public acceptance. The PA-NbS highlights the roles of trust, competing interests, and effectiveness of NbS, as well as public perceptions of risk, nature and place. A lack of consideration of these factors may lead to misaligned public expectations and failed participatory initiatives, while jeopardizing the success of NbS projects. Therefore, as a second step, we conducted citizen surveys within three NbS host communities. Distinct NbS being implemented within the OPERANDUM project aim to reduce risk from (socio-)natural hazards in Scotland (landslides and coastal erosion), Finland (eutrophication and algal blooms) and Greece (river flooding). Associations of factors related to risk, nature, and place perceptions with pro-NbS attitudes and behavior were tested to determine how these may be leveraged to increase acceptance. We find that trust is a consistent factor for defining attitudes towards the NbS across the sites. Attitudes are strongly associated with respondents’ commitment to nature and concern for risk, while some skepticism of NbS effectiveness leads to high public demand for relevant evidence. Risk perception and particularly the potential for future impacts are associated with behavioural acceptance (i.e. willingness to engage), along with responsibility for nature and connectedness to place. Current data collection efforts to demonstrate NbS effectiveness for risk reduction are well-positioned to increase public acceptance in Europe. However, recognizing the differences among segments of the public within each site along with distinct hazard types, proposed NbS, and historical, social, and cultural characteristics across the sites is crucial for designing strategies that increase acceptance. An overview of these dynamics leads to evidence-based recommendations for the case-study sites and for successful NbS in Europe

Public acceptance of nature-based solutions for natural hazard risk reduction: survey findings from three study sites in europe

Climate change is one factor increasing the risk of hydro-meteorological hazards globally. The use of nature-based solutions (NbS), and more specifically ecosystem-based disaster risk reduction measures (Eco-DRR), has become a popular response for risk reduction that also provides highly-valued co-benefits. Public acceptance is of particular importance for NbS since they often rely on local collaborative implementation, management, and monitoring, as well as long-term protection against competing societal interests. Although public engagement is a common goal of NbS projects, it is rarely carried out with a sufficient understanding of the (de)motivating factors tied to public perceptions. Successful collaboration demands consideration of societal attitudes and values in relation to risk, nature, and place. However, existing research does not sufficiently explore these themes together, their interactions, and their implications for the public acceptance of NbS. This may lead to misaligned public expectations and failed participatory initiatives, while jeopardizing the success of NbS projects and their continued funding and uptake. We conducted citizen surveys within local NbS “host” communities to determine the degree of pro-NbS attitudes and behavior, associated variables, and how these may be leveraged to increase acceptance. We compared results across sites, relying primarily on correlations and regression models along with survey comments and expert knowledge. Three distinct rural NbS being implemented within the OPERANDUM project aim to reduce risk from (socio-)natural hazards in Scotland (landslides and coastal erosion; n = 66 respondents), Finland (eutrophication and algal blooms; n = 204) and Greece (river flooding and water scarcity; n = 84). Our research thus centers on rural NbS for risk reduction within a large EU project. Trust in implementers is a consistent factor for defining attitudes towards the NbS across the sites, and attitudes are strongly associated with respondents’ commitment to nature and behavioral acceptance (i.e., willingness to engage). Behaviorial acceptance is most consistently predicted by connectedness to place and the extent of expected future impacts. Skepticism of NbS effectiveness leads to high public demand for relevant evidence. To increase public acceptance, we recommend greater framing of NbS in relation to place-based values as well as demonstration of the effectiveness of NbS for risk reduction. However, distinct hazard types, proposed NbS, and historical characteristics must be considered for developing strategies aimed at increasing acceptance. An understanding of these characteristics and their interactions leads to evidence-based recommendations for our study sites and for successful NbS deployment in Europe and beyond

Microglia regulate myelin growth and integrity in the central nervous system

Myelin is required for the function of neuronal axons in the central nervous system, but the mechanisms that support myelin health are unclear. Although macrophages in the central nervous system have been implicated in myelin health(1), it is unknown which macrophage populations are involved and which aspects they influence. Here we show that resident microglia are crucial for the maintenance of myelin health in adulthood in both mice and humans. We demonstrate that microglia are dispensable for developmental myelin ensheathment. However, they are required for subsequent regulation of myelin growth and associated cognitive function, and for preservation of myelin integrity by preventing its degeneration. We show that loss of myelin health due to the absence of microglia is associated with the appearance of a myelinating oligodendrocyte state with altered lipid metabolism. Moreover, this mechanism is regulated through disruption of the TGFβ1–TGFβR1 axis. Our findings highlight microglia as promising therapeutic targets for conditions in which myelin growth and integrity are dysregulated, such as in ageing and neurodegenerative disease(2,3)

Exploring the potential of civic engagement to strengthen mental health systems in Indonesia (IGNITE) : a study protocol

Background Indonesia has the highest rate of years of life lost to disability or early death from Schizophrenia than any other country in the world. More than 90% of people with mental illness do not get any treatment and tens of thousands of people with psychosis are illegally detained ('pasung') in the family home. Civic engagement, a core part of the recent World Health Organisation global strategy, has the potential to address some of these challenges through the development of person-centered models of care. The aim of the study is to develop a testable systems level, culturally appropriate, civic engagement framework for use in Jakarta and Bogor, Indonesia to strengthen local mental health services. Methods A mixed methods study underpinned by a realist approach will be undertaken across four phases in two study sites in Indonesia (Jakarta and Bogor). Phase 1 will explore the use of civic engagement across South East Asia by conducting a systematic review of existing evidence. By surveying 300 mental health professionals, phase 2 will identify the stakeholders, the sources of collaboration and the evidence used by professionals in decision making within local mental health systems and identify potential opportunities for civic engagement within the system. In order to explore the potential use of civic engagement within Indonesian mental health services and identify priorities for a culturally appropriate framework, phase 3 will undertake two focus groups with participants with experience of psychosis or caring for someone with psychosis (n = 20–30). Professionals and other key decision makers in a range of roles across the system at a national (n = 5) and local level (n = 10–15/site) will also take part in semi-structured interviews. Phase 4 will co-produce a civic engagement framework for use in Indonesia by synthesising evidence from phases 1–3 collaboratively with key stakeholders. Discussion Civic engagement is a potential way in which health services in low and middle income countries can address the burden of mental health conditions through the development of person-centred models of care. However, such approaches are underexplored in Indonesia. This study will work with local stakeholders to design a testable civic engagement framework for use in mental health services in Indonesia

Engineering Yarrowia lipolytica to Produce Glycoproteins Homogeneously Modified with the Universal Man3GlcNAc2 N-Glycan Core

Yarrowia lipolytica is a dimorphic yeast that efficiently secretes various heterologous proteins and is classified as “generally recognized as safe.” Therefore, it is an attractive protein production host. However, yeasts modify glycoproteins with non-human high mannose-type N-glycans. These structures reduce the protein half-life in vivo and can be immunogenic in man. Here, we describe how we genetically engineered N-glycan biosynthesis in Yarrowia lipolytica so that it produces Man3GlcNAc2 structures on its glycoproteins. We obtained unprecedented levels of homogeneity of this glycanstructure. This is the ideal starting point for building human-like sugars. Disruption of the ALG3 gene resulted in modification of proteins mainly with Man5GlcNAc2 and GlcMan5GlcNAc2 glycans, and to a lesser extent with Glc2Man5GlcNAc2 glycans. To avoid underoccupancy of glycosylation sites, we concomitantly overexpressed ALG6. We also explored several approaches to remove the terminal glucose residues, which hamper further humanization of N-glycosylation; overexpression of the heterodimeric Apergillus niger glucosidase II proved to be the most effective approach. Finally, we overexpressed an α-1,2-mannosidase to obtain Man3GlcNAc2 structures, which are substrates for the synthesis of complex-type glycans. The final Yarrowia lipolytica strain produces proteins glycosylated with the trimannosyl core N-glycan (Man3GlcNAc2), which is the common core of all complex-type N-glycans. All these glycans can be constructed on the obtained trimannosyl N-glycan using either in vivo or in vitro modification with the appropriate glycosyltransferases. The results demonstrate the high potential of Yarrowia lipolytica to be developed as an efficient expression system for the production of glycoproteins with humanized glycans

Why Are Outcomes Different for Registry Patients Enrolled Prospectively and Retrospectively? Insights from the Global Anticoagulant Registry in the FIELD-Atrial Fibrillation (GARFIELD-AF).

Background: Retrospective and prospective observational studies are designed to reflect real-world evidence on clinical practice, but can yield conflicting results. The GARFIELD-AF Registry includes both methods of enrolment and allows analysis of differences in patient characteristics and outcomes that may result. Methods and Results: Patients with atrial fibrillation (AF) and ≥1 risk factor for stroke at diagnosis of AF were recruited either retrospectively (n = 5069) or prospectively (n = 5501) from 19 countries and then followed prospectively. The retrospectively enrolled cohort comprised patients with established AF (for a least 6, and up to 24 months before enrolment), who were identified retrospectively (and baseline and partial follow-up data were collected from the emedical records) and then followed prospectively between 0-18 months (such that the total time of follow-up was 24 months; data collection Dec-2009 and Oct-2010). In the prospectively enrolled cohort, patients with newly diagnosed AF (≤6 weeks after diagnosis) were recruited between Mar-2010 and Oct-2011 and were followed for 24 months after enrolment. Differences between the cohorts were observed in clinical characteristics, including type of AF, stroke prevention strategies, and event rates. More patients in the retrospectively identified cohort received vitamin K antagonists (62.1% vs. 53.2%) and fewer received non-vitamin K oral anticoagulants (1.8% vs . 4.2%). All-cause mortality rates per 100 person-years during the prospective follow-up (starting the first study visit up to 1 year) were significantly lower in the retrospective than prospectively identified cohort (3.04 [95% CI 2.51 to 3.67] vs . 4.05 [95% CI 3.53 to 4.63]; p = 0.016). Conclusions: Interpretations of data from registries that aim to evaluate the characteristics and outcomes of patients with AF must take account of differences in registry design and the impact of recall bias and survivorship bias that is incurred with retrospective enrolment. Clinical Trial Registration: - URL: http://www.clinicaltrials.gov . Unique identifier for GARFIELD-AF (NCT01090362)