Inhibition of cell proliferation, migration and colony formation of LS174T Cells by carbonic anhydrase inhibitor

Background: Metastasis is the leading cause of cancer deaths. Migration of tumor cells is an important stage in metastasis. Therefore, recent studies have focused on clarifying migration and migration-dependent cell functions such as angiogenesis, wound healing, and invasion. Objectives: In the present study, we aimed to investigate the effect of acetazolamide, which is a classical carbonic anhydrase inhibitor, on the cell viability, migration, and colony forming capacity of human LS174T colorectal cancer cells. Methods: Three different cell culture techniques (MTT test, wound healing and clonogenic assay) were performed in this in vitro study on colorectal cancer cells. Results: Acetazolamide reduced the cell viability, migration and colony formation ability of cells depending on dose. There was no significant difference between the cells treated with acetazolamide with 1 \u3bcM dose and the control. However, it can be concluded that acetazolamide exerts its effect on human colorectal cancer cells at 10-1000 \ub5M concentrations. Conclusion: Acetazolamide was observed to significantly inhibit the cell viability, colony forming capacity, and migration ability in the culture medium of LS174T cells. This inhibitor effect of acetazolamide was observed to be dependent on the concentration in medium

The effects of docking on performance and carcass characteristics of male Karaka lambs

The effects of tail docking at birth on growth performance and carcass characteristics of fat-tailed male Karakas lambs were investigated. A total of 23 Karakas single-born male lambs was used in this study. Nine were docked at one day of age using rubber elastrator rings and the rest was left intact. After weaning, the lambs were fed a finishing diet for 70 days, and then slaughtered to evaluate carcass characteristics. Total weight gains during the finishing period were 14.3 + 1.00 and 18.9 + 0.82 kg and average daily live weight gains 204.7 + 14.41 and 269.9 + 11.72 g for the undocked vs. the docked group, respectively. The carcasses of the docked group contained more kidney, pelvic and internal fat than the intact lambs as well as a higher percentage of subcutaneous and intramuscular fat. The weights of the different carcass cuts of the docked lambs were also heavier that those of the intact group. The results indicated that the tail docking of fat-tailed Karakas lambs at birth improved weight gain and desirable carcass characteristics compared to those of undocked lambs. Keywords: Karakas lambs, Fat tail docking, Growth performances, Slaughter South African Journal of Animal Science Vol.33(3) 2003: 185-19

Examining the method of proofs and refutations in pre-service teachers education

There is some evidence in the mathematics education literature that Lakatos' proofs and refutation methods can be useful to examining students' conjecture production and proof construction process. The purpose of this study was to determine how the Lakatos method goes and which steps of the method works in the teacher education program. The population sample for this study consists of 24 senior pre-service teachers in elementary mathematics education in Turkey (16 women and 8 men). Pre-service teachers were given a problem in which they examined the relation between perimeter and area of a rectangle. Data was collected with a camera, field notes, and groups' written solutions and analyzed on the basis of framework included in Larsen and Zandieh's (2008) study. The finding revealed that Lakatos' method was usable in the teacher education program. But some steps of the method described in Lakatos' (1976) historical case study were not provided in the real classroom environment

Crystal And Molecular Structures Of Trans-Nickel(Ii)-Bis[(O-Propyl(N))(P-Methoxyphenyl)Dithiophosphonate]

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