3D reconstruction of magnetization from dichroic soft X-ray transmission tomography

The development of magnetic nanostructures for applications in spintronics requires methods capable of visualizing their magnetization. Soft X‐ray magnetic imaging combined with circular magnetic dichroism allows nanostructures up to 100–300 nm in thickness to be probed with resolutions of 20–40 nm. Here a new iterative tomographic reconstruction method to extract the three‐dimensional magnetization configuration from tomographic projections is presented. The vector field is reconstructed by using a modified algebraic reconstruction approach based on solving a set of linear equations in an iterative manner. The application of this method is illustrated with two examples (magnetic nano‐disc and micro‐square heterostructure) along with comparison of error in reconstructions, and convergence of the algorithm

Purification and genetic characterization of gassericin E, a novel co-culture inducible bacteriocin from Lactobacillus gasseri EV1461 isolated from the vagina of a healthy woman

This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) through the Project AGL2012-33400 and AGL2013-41980-P, and by the Junta de Andalucía Excellence Project AGR-07345. These projects included FEDER funds. AMB was the recipient of a post-doctoral grant awarded by the Junta de Andalucía as part of the Project AGR-07345.Background: Lactobacillus gasseri is one of the dominant Lactobacillus species in the vaginal ecosystem. Some strains of this species have a high potential for being used as probiotics in order to maintain vaginal homeostasis, since they may confer colonization resistance against pathogens in the vagina by direct inhibition through production of antimicrobial compounds, as bacteriocins. In this work we have studied bacteriocin production of gassericin E (GasE), a novel bacteriocin produced by L. gasseri EV1461, a strain isolated from the vagina of a healthy woman, and whose production was shown to be promoted by the presence of certain specific bacteria in co-culture. Biochemical and genetic characterization of this novel bacteriocin are addressed. Results: We found that the inhibitory spectrum of L. gasseri EV1461 was broad, being directed to species both related and non-related to the producing strain. Interestingly, L. gasseri EV1461 inhibited the grown of pathogens usually associated with bacterial vaginosis (BV). The antimicrobial activity was due to the production of a novel bacteriocin, gassericin E (GasE). Production of this bacteriocin in broth medium only was achieved at high cell densities. At low cell densities, bacteriocin production ceased and only was restored after the addition of a supernatant from a previous bacteriocin-producing EV1461 culture (autoinduction), or through co-cultivation with several other Gram-positive strains (inducing bacteria). DNA sequence of the GasE locus revealed the presence of two putative operons which could be involved in biosynthesis and immunity of this bacteriocin (gaeAXI), and in regulation, transport and processing (gaePKRTC). The gaePKR encodes a putative three-component regulatory system, involving an autoinducer peptide (GaeP), a histidine protein kinase (GaeK) and a response regulator (GaeR), while the gaeTC encodes for an ABC transporter (GaeT) and their accessory protein (GaeC), involved in transport and processing of the bacteriocin. The gaeAXI, encodes for the bacteriocin gassericin E (GasE), a putative peptide bacteriocin (GaeX), and their immunity protein (GaeI). Conclusions: The origin of the strain (vagina of healthy woman) and its ability to produce bacteriocins with inhibitory activity against vaginal pathogens may be an advantage for using L. gasseri EV1461 as a probiotic strain to fight and/or prevent bacterial infections as bacterial vaginosis (BV), since it could be better adapted to live and compete into the vaginal environment.Publisher PDFPeer reviewe

Empirical Wavelet Transform-based Detection of Anomalies in ULF Geomagnetic Signals Associated to Seismic Events with a Fuzzy Logic-based System for Automatic Diagnosis

Owing to the relevance and severity of damages caused by earthquakes (EQs), the development and application of new methods for seismic activity detection that offer an efficient and reliable diagnosis in terms of processing and performance are still demanding tasks. In this work, the application of the Empirical Wavelet Transform (EWT) for seismic detection in ultra-low-frequency (ULF) geomagnetic signals is presented. For this, several ULF signals associated to seismic activities and random calm periods are analysed. These signals have been obtained through a tri-axial fluxgate magnetometer at the Juriquilla station localized in Queretaro, Mexico, longitude -100.45° N and latitude 20.70°E. In order to show the advantages of the proposal, a comparison with the discrete wavelet transform (DWT) is presented. The results shown a better detection capability of seismic signals before, during, and after the main shock than the ones obtained by the DWT, which makes the proposal a more suitable and reliable tool for this task. Finally, a fuzzy logic (FL)-based system for automatic diagnosis using the variance of the EWT outputs for the tri-axial fluxgate magnetometer signals is also proposed

A1298C polymorphism in the MTHFR gene predisposes to cardiovascular risk in rheumatoid arthritis

8 páginas, 1 figura, 3 tablas.-- et al.[Introduction]: We determined the contribution of the methylene tetrahydrofolate reductase (MTHFR) 677 C>T and 1298 A>C gene polymorphisms to the susceptibility to rheumatoid arthritis (RA). We also assessed whether these two MTHFR gene polymorphisms may be implicated in the development of cardiovascular (CV) events and subclinical atherosclerosis manifested by the presence of endothelial dysfunction, in a series of Spanish patients with RA. [Methods]: Six hundred and twelve patients fulfilling the 1987 American College of Rheumatology classification criteria for RA, seen at the rheumatology outpatient clinics of Hospital Xeral-Calde, Lugo and Hospital San Carlos, Madrid, were studied. Patients and controls (n = 865) were genotyped using predesigned TaqMan SNP genotyping assays. [Results]: No significant differences in allele or genotype frequencies for the MTHFR gene polymorphisms between RA patients and controls were found. Also, no association between the MTHFR 677 C>T polymorphism and CV events or endothelial dysfunction was observed. However, the MTHFR 1298 allele C frequency was increased in patients with CV events after 5 years (38.7% versus 30.3%; odds ratio = 1.45; 95% confidence interval = 1.00 to 2.10; P = 0.04) and 10 years (42.2% versus 31.0%; odds ratio = 1.62; 95% confidence interval = 1.08 to 2.43; P = 0.01) follow up. Moreover, patients carrying the MTHFR 1298 AC and CC genotypes had a significantly decreased flow-mediated endothelium-dependent vasodilatation (4.3 ± 3.9%) compared with those carrying the MTHFR 1298 AA genotype (6.5 ± 4.4%) (P = 0.005). [Conclusions]: Our results show that the MTHFR 1298 A>C gene polymorphism confers an increased risk for subclinical atherosclerosis and CV events in patients with RA.The present study was supported by two grants from Fondo de Investigaciones Sanitarias PI06-0024 and PS09/00748 (Spain). This work was partially supported by RETICS Program RD08/0075 (RIER) from the Instituto de Salud Carlos III.Peer reviewe

Synthesis by wet chemistry and characterization of LiNbO3 nanoparticles

Actually, lithium niobate (LiNbO3) has been used for optical wavelength conversion and ultrafast optical signal processing because of its outstanding rapid nonlinear optical response behavior, low switching power and broad conversion bandwidth. LiNbO3nanoparticles, which belong to the ferroelectric oxide class, were synthesized by chemical reaction with wetchemistry. Their sizedistributionwascenteredaround200 nm. Xray diffraction (XRD) and scanning electron microscopy (SEM) were used to further investigate the quality of the obtained LiNbO3powders.The present work shows thatby employingthis chemical method the correct stoichiometric phasewas obtained. This wascorroborated by XPS (X-Ray Photoelectron Spectroscopy) results. Also, the nanoparticles showed a defined crystallinity and uniform morphology. This way of obtaining nanoparticles is innovative because of its low cost and simple way to reproduce it. It isan important method of increasing the surfacearea, controlling thephase purityand reducing theparticle size distribution. The samples were obtained under low temperature annealing at500, 650 and 800 ºC. Those features can be controlled using variables such temperature, time of synthesis,and calcination. In previous worksit wasfound that hydrothermal methods offer many advantages over conventional ceramic synthesis methods

Effect of Natural Turf, Artificial Turf, and Sand Surfaces on Sprint Performance. A Systematic Review and Meta-Analysis

The aim of this study was to analyze the influence of natural turf, artificial turf, and sand on sprint performance in different sports and to determine how the sport surface affects sprint performance. A systematic search was conducted in Pubmed, Web of Sciences, and SPORTDiscus databases. Out of 5644 studies, 11 studies were included in the meta-analysis. The studies were very heterogeneous, as they examined different structural characteristics or quality parameters. The studies on natural turf and sand showed significant improvements on sprint speed during training. On the other hand, the analysis of fatigue did not reveal significant differences in the deterioration of sprint speed on both natural and artificial turf. Significance was set at p < 0.05. In conclusion, although lower performance in sprint was reported on sand, further studies are needed to explain the differences in sprint on natural and artificial turf

The Effects of Football Practice on Nutritional Status and Body Composition in Children: A Systematic Review and Meta-Analysis

Dietary patterns, exercise, sport, and physical activity have been shown to improve body composition in children. This systematic review with meta-analysis analyzed the effects of practicing football on body composition (fat mass, lean body mass, and bone mineral content) in children. An initial search in PubMed, Web of Science, and SPORTDiscus was carried out in April 2021 to identify relevant articles. Inclusion criteria required children up to 12 years of age with a minimum football intervention duration of 10 weeks. Methodological quality of the articles was evaluated using the PEDro scale. Between the 1803 articles originally founded, only 14 articles were included in the meta-analysis. A total of 1643 subjects between the 14 studies were identified. The review and meta-analysis were conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and used Review Manager and Full Meta-Analysis software. The results between the control and experimental groups showed significantly better lean body mass and fat mass values in the experimental group (p < 0.05). Football practice was positively associated with increases in lean body mass (mean difference of 1.55; 95% CI, 0.96, 2.15), decreases in fat mass (mean difference of −0.81; 95% CI, −1.49, −0.13), and increases in whole body bone mineral content (mean difference of 117.68; 95% CI, 83.69, 151.67). In conclusion, the results of this systematic review with meta-analysis suggest that football positively affects body composition in children. However, further research is needed to confirm the results for bone mineral content.A.H.-M. acknowledges the Spanish Ministry of Science, Innovation and Universities for funding the development of his PhD. (Grant no.: FPU18/03222). S.M.-C. acknowledges the University of Castilla-La Mancha for funding the development of his PhD. (2019/5964). J.G.-U. acknowledges “Fondo Europeo de Desarrollo Regional, Programa Operativo de la Región de Castilla-La Mancha” (2018/11744) for funding the development of his research

Association of acid phosphatase locus 1*C allele with the risk of cardiovascular events in rheumatoid arthritis patients

Introduction: Acid phosphatase locus 1 (ACP1) encodes a low molecular weight phosphotyrosine phosphatase implicated in a number of different biological functions in the cell. The aim of this study was to determine the contribution of ACP1 polymorphisms to susceptibility to rheumatoid arthritis (RA), as well as the potential contribution of these polymorphisms to the increased risk of cardiovascular disease (CV) observed in RA patients. Methods: A set of 1,603 Spanish RA patients and 1,877 healthy controls were included in the study. Information related to the presence/absence of CV events was obtained from 1,284 of these participants. All individuals were genotyped for four ACP1 single-nucleotide polymorphisms (SNPs), rs10167992, rs11553742, rs7576247, and rs3828329, using a predesigned TaqMan SNP genotyping assay. Classical ACP1 alleles (*A, *B and *C) were imputed with SNP data. Results: No association between ACP1 gene polymorphisms and susceptibility to RA was observed. However, when RA patients were stratified according to the presence or absence of CV events, an association between rs11553742*T and CV events was found (P = 0.012, odds ratio (OR) = 2.62 (1.24 to 5.53)). Likewise, the ACP1*C allele showed evidence of association with CV events in patients with RA (P = 0.024, OR = 2.43). Conclusions: Our data show that the ACP1*C allele influences the risk of CV events in patients with R

Personalized Management of Fatigue in Individuals With Myalgic Encephalomyelitis/Chronic Fatigue Syndrome and Long COVID Using a Smart Digital mHealth Solution: Protocol for a Participatory Design Approach

Fatigue; Myalgic encephalomyelitis/chronic fatigue syndrome; Personalized self-managementFatiga; Encefalomielitis miálgica/síndrome de fatiga crónica; Autogestión personalizadaFatiga; Encefalomielitis miàlgica/síndrome de fatiga crònica; Autogestió personalitzadaBackground: Fatigue is the most common symptom in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) and long COVID, impacting patients’ quality of life; however, there is currently a lack of evidence-based context-aware tools for fatigue self-management in these populations. Objective: This study aimed to (1) address fatigue in ME/CFS and long COVID through the development of digital mobile health solutions for self-management, (2) predict perceived fatigue severity using real-time data, and (3) assess the feasibility and potential benefits of personalized digital mobile health solutions. Methods: The MyFatigue project adopts a patient-centered approach within the participatory health informatics domain. Patient representatives will be actively involved in decision-making processes. This study combines inductive and deductive research approaches, using qualitative studies to generate new knowledge and quantitative methods to test hypotheses regarding the relationship between factors like physical activity, sleep behaviors, and perceived fatigue in ME/CFS and long COVID. Co-design methods will be used to develop a personalized digital solution for fatigue self-management based on the generated knowledge. Finally, a pilot study will evaluate the feasibility, acceptance, and potential benefits of the digital health solution. Results: The MyFatigue project opened to enrollment in November 2023. Initial results are expected to be published by the end of 2024. Conclusions: This study protocol holds the potential to expand understanding, create personalized self-management approaches, engage stakeholders, and ultimately improve the well-being of individuals with ME/CFS and long COVID.This study received funding from the Spanish Ministry of Science and Innovation (Knowledge Generation Project Grant PID-2021-125528OB-I00), and it was cofunded by the 2020 European Union’s Research and Innovation Funding Program