299 research outputs found
Deppining of a Superfluid Vortex Inside a Circular Defect
In this work we study the process of depinning of a quantum of circulation
trapped inside a disk by an applied two dimensional superflow. We use the
Gross-Pitaevskii model to describe the neutral superfluid. The collective
coordinate dynamics is derived directly from the condensate equation of motion,
the nonlinear Schroedinger equation, and it is used to obtain an expression for
the critical velocity as a function of the defect radius. This expression is
compared with a numerical result obtained from the time independent nonlinear
Schroedinger equation. Below the critical velocity, we obtain the dependence of
the semiclassical nucleation rate with the flow velocity at infinity. Above the
critical velocity, the classical vortex depinning is illustrated with a
numerical simulation of the time dependent nonlinear Schroedinger equation.Comment: 8 pages, 5 figures, uses revtex and epsf.st
one-step extraction and separation of betalains and chlorophylls using thermoreversible aqueous biphasic systems
This work was partly developed within the scope of the projects CICECO-Aveiro Institute of Materials, LA/P/0006/2020 and the Associate Laboratory for Green Chemistry-LA/P/0008/2020, financed by national funds through the FCT/MCTES (PIDDAC).
Publisher Copyright:
© 2023 The Royal Society of Chemistry.Globally, up to 50% of root crops, fruits and vegetables produced is wasted. Beetroot stems and leaves fit into this scenario, with only a small fraction being used in cattle food. One way of approaching this problem is through their valorisation, by extracting and recovering valuable compounds present in this type of waste that could be used in other applications, while contributing towards a circular economy. In this work, a new integrated process using thermoreversible aqueous biphasic systems (ABS) composed of quaternary ammonium-based ionic liquids (ILs) and polypropyleneglycol 400 g mol−1 (PPG) is shown to allow the one-step extraction and separation of two pigment classes—betalains and chlorophylls—from red beet stems and leaves. The pigment extraction was carried out with a monophasic aqueous solution of the IL and PPG, whose phase separation was then achieved by a temperature switch, resulting in the simultaneous separation of chlorophylls and betalains into opposite phases. A central composite design was used to optimise the extraction parameters (time, temperature, and solid : liquid (S/L) ratio) of both pigment extraction yields, reaching at 20 °C, 70 min and a S/L ratio of 0.12 a maximum extraction yield of 6.67 wt% for betalains and 1.82 wt% for chlorophylls (per weight of biomass). Moreover, it is shown that aqueous solutions of ILs better stabilise betalains than the gold standard solvent used for the extraction method. Among the studied systems, the ABS comprising the IL N-ethyl-N-methyl-N,N-bis(2-hydroxyethyl) bromide ([N21(2OH)(2OH)]Br) presented the best separation performance, with an extraction efficiency of 92% and 95% for chlorophylls and betalains, respectively, for opposite phases. The pigments were removed from the respective phases using affinity resins, with high recoveries: 96% for betalains and 98% for chlorophylls, further allowing the IL reuse. Finally, the cyto- and ecotoxicities of the quaternary ammonium-based ILs were determined. The obtained results disclosed low to negligible toxicity in the thousands of mg L−1 range, with [N21(2OH)(2OH)]Br being harmless from an ecotoxicological point of view. Overall, it is shown here that the developed process is an innovative approach for the one-step extraction and selective separation of pigments contributing to the valorisation of waste biomass.publishersversionpublishe
Image processing techniques applied to concrete’s porosity determination / Técnicas de processamento de imagem aplicadas à determinação da porosidade do concreto
A porosidade é uma das propriedades dos concretos que está associada à sua qualidade por influenciar diretamente na sua resistência. A microtomografia é uma técnica não destrutiva para caracterização de materiais que permite a obtenção de imagens da sua estrutura interna. Informações sobre esta estrutura interna podem ser extraídas e analisadas utilizando técnicas de processamento e análise de imagens. O objetivo deste trabalho é implementar uma metodologia que, usando técnicas de processamento de imagens, permita analisar imagens geradas por microtomografia computadorizada com o intuito de determinar a porosidade em concretos
ATP - bioluminescence as a technique to evaluate the microbiological quality of water in food industry
A genome-wide association study follow-up suggests a possible role for PPARG in systemic sclerosis susceptibility
Introduction:
A recent genome-wide association study (GWAS) comprising a French cohort of systemic sclerosis (SSc) reported several non-HLA single-nucleotide polymorphisms (SNPs) showing a nominal association in the discovery phase. We aimed to identify previously overlooked susceptibility variants by using a follow-up strategy.<p></p>
Methods:
Sixty-six non-HLA SNPs showing a P value <10-4 in the discovery phase of the French SSc GWAS were analyzed in the first step of this study, performing a meta-analysis that combined data from the two published SSc GWASs. A total of 2,921 SSc patients and 6,963 healthy controls were included in this first phase. Two SNPs, PPARG rs310746 and CHRNA9 rs6832151, were selected for genotyping in the replication cohort (1,068 SSc patients and 6,762 healthy controls) based on the results of the first step. Genotyping was performed by using TaqMan SNP genotyping assays.
Results:
We observed nominal associations for both PPARG rs310746 (PMH = 1.90 × 10-6, OR, 1.28) and CHRNA9 rs6832151 (PMH = 4.30 × 10-6, OR, 1.17) genetic variants with SSc in the first step of our study. In the replication phase, we observed a trend of association for PPARG rs310746 (P value = 0.066; OR, 1.17). The combined overall Mantel-Haenszel meta-analysis of all the cohorts included in the present study revealed that PPARG rs310746 remained associated with SSc with a nominal non-genome-wide significant P value (PMH = 5.00 × 10-7; OR, 1.25). No evidence of association was observed for CHRNA9 rs6832151 either in the replication phase or in the overall pooled analysis.<p></p>
Conclusion:
Our results suggest a role of PPARG gene in the development of SSc
Institutional assessment as an instrument of rationalization back to the school as a formal organisation
O papel central da avaliação, da avaliação institucional e dos
processos de garantia da qualidade de escolas e universidades
nas políticas educacionais é objeto de discussão, também
considerando a reforma do Estado. As teorias da nova gestão
pública e as perspectivas gestionárias são consideradas como
algumas das principais fontes de inspiração e de legitimação no
contexto dominante de uma educação contábil. Dados empíricos
preliminares resultantes do processo de avaliação externa de
escolas básicas e secundárias portuguesas são apresentados
e interpretados de acordo com os principais conceitos e
representações organizacionais de escola presentes nos relatórios
externos. Examinando algumas das imagens e dos significados de
escola, cultura de escola, autonomia, objetivos, liderança e eficácia
presentes nos relatórios de avaliação, o autor releva a importância
das imagens formais, racionais e burocráticas de escolas. Várias
questões de investigação são apresentadas tendo por base aquilo
a que o autor chama o processo da hiperburocratização das
organizações educativas. Algumas dimensões do conceito de
burocracia de Max Weber são revisitadas, em articulação com
perspectivas neocientíficas de garantia da qualidade e com as
tecnologias da informação e comunicação. O autor sugere que
maior relevância deve ser atribuída aos modelos formais e racionais
de interpretação das organizações educativas, pois os processos de
avaliação e de garantia da qualidade estão a contribuir para a
formalização de escolas e universidades e para a intensificação do
seu processo de racionalização, isto é, para a emergência de uma
imagem analítica das escolas como hiperburocracias.The central role of evaluation, institutional assessment and quality
assurance processes of schools and universities in education policy
is object of discussion also considering the reform of the state. New
Public Management theories and managerialist perspectives are
considered some of the main sources of inspiration and legitimation
in the dominant context of an audit education. Preliminary
empirical data from external assessment of Portuguese primary and
secondary schools are introduced and interpreted according to the
main concepts and organizational representations of school found
in external reports. Examining some of the central organizational
images and meanings of school, school culture, autonomy, goals,
leadership and effectiveness included in the external reviews, the
author stresses the importance of formal, rational and bureaucratic
images of schools. Several new research questions are presented
for further inquiry based on the hypothesis of what it is called by
the author the process of hyperbureaucratization of educational
organizations. Some of the main dimensions of the concept of
bureaucracy as presented by Max Weber are revisited in close
relation with neo-scientific approaches of quality assurance and
taking in consideration the use of information and communication
technologies. Accordingly the author suggests that much more
importance must be given to formal and rational models of
interpreting educational organizations because assessment and
quality assurance procedures are contributing to the formalization
of schools and universities and to the intensification of their process
of rationalization, i. e., to the emergence of an analytic image of
schools as hyperbureaucracies.(undefined
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Association of Genetic Variants With Primary Open-Angle Glaucoma Among Individuals With African Ancestry.
Importance:Primary open-angle glaucoma presents with increased prevalence and a higher degree of clinical severity in populations of African ancestry compared with European or Asian ancestry. Despite this, individuals of African ancestry remain understudied in genomic research for blinding disorders. Objectives:To perform a genome-wide association study (GWAS) of African ancestry populations and evaluate potential mechanisms of pathogenesis for loci associated with primary open-angle glaucoma. Design, Settings, and Participants:A 2-stage GWAS with a discovery data set of 2320 individuals with primary open-angle glaucoma and 2121 control individuals without primary open-angle glaucoma. The validation stage included an additional 6937 affected individuals and 14 917 unaffected individuals using multicenter clinic- and population-based participant recruitment approaches. Study participants were recruited from Ghana, Nigeria, South Africa, the United States, Tanzania, Britain, Cameroon, Saudi Arabia, Brazil, the Democratic Republic of the Congo, Morocco, Peru, and Mali from 2003 to 2018. Individuals with primary open-angle glaucoma had open iridocorneal angles and displayed glaucomatous optic neuropathy with visual field defects. Elevated intraocular pressure was not included in the case definition. Control individuals had no elevated intraocular pressure and no signs of glaucoma. Exposures:Genetic variants associated with primary open-angle glaucoma. Main Outcomes and Measures:Presence of primary open-angle glaucoma. Genome-wide significance was defined as P < 5 × 10-8 in the discovery stage and in the meta-analysis of combined discovery and validation data. Results:A total of 2320 individuals with primary open-angle glaucoma (mean [interquartile range] age, 64.6 [56-74] years; 1055 [45.5%] women) and 2121 individuals without primary open-angle glaucoma (mean [interquartile range] age, 63.4 [55-71] years; 1025 [48.3%] women) were included in the discovery GWAS. The GWAS discovery meta-analysis demonstrated association of variants at amyloid-β A4 precursor protein-binding family B member 2 (APBB2; chromosome 4, rs59892895T>C) with primary open-angle glaucoma (odds ratio [OR], 1.32 [95% CI, 1.20-1.46]; P = 2 × 10-8). The association was validated in an analysis of an additional 6937 affected individuals and 14 917 unaffected individuals (OR, 1.15 [95% CI, 1.09-1.21]; P < .001). Each copy of the rs59892895*C risk allele was associated with increased risk of primary open-angle glaucoma when all data were included in a meta-analysis (OR, 1.19 [95% CI, 1.14-1.25]; P = 4 × 10-13). The rs59892895*C risk allele was present at appreciable frequency only in African ancestry populations. In contrast, the rs59892895*C risk allele had a frequency of less than 0.1% in individuals of European or Asian ancestry. Conclusions and Relevance:In this genome-wide association study, variants at the APBB2 locus demonstrated differential association with primary open-angle glaucoma by ancestry. If validated in additional populations this finding may have implications for risk assessment and therapeutic strategies
Prophylactic and Therapeutic Use of Strontium Ranelate Reduces the Progression of Experimental Osteoarthritis
Introduction: Strontium ranelate (SrRan) has the potential to interfere in the progression of osteoarthritis (OA), multifactorial disease associated with mechanical problems and articular inflammatory changes.Objectives: This study aimed to test the effects of prophylactic and therapeutic use of SrRan on clinical parameters of pain, the inflammatory process, and degradation of the articular cartilage.Methods: This was an experimental study, using a model of knee OA induced by intra-articular injection of monoiodoacetate. Thirty Wistar rats were divided into five groups and treated as indicated: control, without intervention; prophylactic, received SrRan at a daily oral dose of 250 mg/kg for 28 days before OA induction; SrRan treatments, administered 250 or 500 mg/kg/day for 28 days after the induction; and model control, received saline solution after the induction. Behavioral tests (joint incapacity, mechanical hyperalgesia, tactile sensitivity, and forced ambulation), histological evaluation of articular cartilage, and determination of inflammatory cytokines in the synovial fluid (interleukin [IL]-6, IL-10, tumor necrosis factor [TNF]-α, and interferon [INF]-γ) were performed.Results: Both prophylactic and therapeutic treatments improved the articular discomfort. A prophylactic dose of 500 mg/kg/day also improved mechanical hyperalgesia and the same dose was beneficial on tactile sensitivity. SrRan did not improve ambulation. Levels of IL-6, IL-10, TNF-α, and IFN-γ in SrRan-treated groups with OA were not significantly different compared with those in the normal control animals. The histopathological evaluation showed less articular damage in the SrRan-treated and control groups compared to the saline-treated group.Conclusion: The prophylactic and therapeutic administration of SrRan was associated with improved behavioral patterns of pain, especially joint discomfort. SrRan administration mitigated histological changes in the articular cartilage and reduced the inflammatory process, which beneficially reduced the progression of OA in the experimental model studied
High prevalence and mortality due to Histoplasma capsulatum in the Brazilian Amazon: An autopsy study
Background: Histoplasmosis is acquired by inhalation of spores of the dimorphic fungus Histoplasma spp. Although this pathogen is distributed worldwide, it is more prevalent in the Americas. However, the real burden of histoplasmosis remains undefined in many endemic regions. Methodology: We conducted a series of 61 autopsies to individuals who died in a hospital in the Brazilian Amazon focused on infectious diseases. We performed a detailed histological and microbiological evaluation with genetic characterization of Histoplasma strains with the aim to evaluate the contribution of histoplasmosis to morbidity and mortality. Additionally, we assessed the clinicopathological correlation. Principal findings: Evidence of Histoplasma infection was detected in 21 patients (34%). Eight cases were disseminated infections, all of them occurred in HIV-positive patients. Six cases were localized histoplasmosis, limited to the lungs. In seven patients Histoplasma DNA was detected by PCR in patients with no histological lesions. Histoplasma infection was detected in 38% of HIV-positive patients and was a major contributor to death in 22% of them. Lungs, liver and spleen were affected in all cases of disseminated histoplasmosis. Phylogenetic analysis of the strains suggested a high diversity of Histoplasma species circulating in the Brazilian Amazon. Histoplasmosis was clinically missed in 75% of the disseminated infections. Conclusions: The high incidence of histoplasmosis, the low index of clinical suspicion, and the severity of the disseminated disease highlight the need of proactively implementing sensitive routine screening methods for this pathogen in endemic areas. Antifungal prophylaxis against Histoplasma should be encouraged in the severely immunocompromised HIV patients in these areas. In conclusion, substantial mortality is associated with disseminated histoplasmosis among HIV-positive patients in the Brazilian Amazon
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