67 research outputs found
Disponibilidade, acesso e apropriação da leitura no contexto do programa "Literatura em Minha Casa"
JABUTICABA PEEL IN THE PRODUCTION OF COOKIES FOR SCHOOL FOOD: TECHNOLOGICAL AND SENSORY ASPECTS
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes.
The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological
substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos
as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene
delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and
mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can
cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the
expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those
embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the
embryonic development. This study shows the potential utility of the MWNT for the development of
new method for delivery of DNA into bovine embryos
Safety and Quality in the Agricultural Product Chain in Brazil
An agriculture-intensive country should be aware of natural toxins, including both mycotoxins and cyanotoxins, which are closely associated with the quality of raw materials, for food safety and industry. The major production chains – corn, wheat, beef, and broiler chicken – are the top components of agribusiness, and they should be tracked by reliable and practical tools. The corn chain is of particular concern in food production; intensive controls, multi-year mycotoxin monitoring, and improved harmless/sustainable management methods for uninterrupted farming in the tropic-subtropics are needed to achieve a long-lasting trend. The rapid control of natural toxins (mycotoxin and cyanotoxin) has focused on immunochemical methods developed with highly specific monoclonal antibodies (mAb) matched with chromatographic methods. In parallel, the promising widespread application of non-destructive analytical methods based on NIR (Near Infrared Reflectance) spectroscopy, computer vision and hyperspectral imaging coupled with multivariate analyses have been introduced as an alternative for the prediction of quality and compositional parameters. Rapid quality control and product traceability are discussed, as well as accurate monitoring, which is essential for potentially launching an innovative system for food production in Brazil
Cell-Free Antigens from Paracoccidioides brasiliensis Drive IL-4 Production and Increase the Severity of Paracoccidioidomycosis
The thermally dimorphic fungus Paracoccidioides brasiliensis (Pb) is the causative agent of paracoccidioidomycosis (PCM), one of the most frequent systemic mycosis that affects the rural population in Latin America. PCM is characterized by a chronic inflammatory granulomatous reaction, which is consequence of a Th1-mediated adaptive immune response. In the present study we investigated the mechanisms involved in the immunoregulation triggered after a prior contact with cell-free antigens (CFA) during a murine model of PCM. The results showed that the inoculation of CFA prior to the infection resulted in disorganized granulomatous lesions and increased fungal replication in the lungs, liver and spleen, that paralleled with the higher levels of IL-4 when compared with the control group. The role of IL-4 in facilitating the fungal growth was demonstrated in IL-4-deficient- and neutralizing anti-IL-4 mAb-treated mice. The injection of CFA did not affect the fungal growth in these mice, which, in fact, exhibited a significant diminished amount of fungus in the tissues and smaller granulomas. Considering that in vivo anti-IL-4-application started one week after the CFA-inoculum, it implicates that IL-4-CFA-induced is responsible by the mediation of the observed unresponsiveness. Further, the characterization of CFA indicated that a proteic fraction is required for triggering the immunosuppressive mechanisms, while glycosylation or glycosphingolipids moieties are not. Taken together, our data suggest that the prior contact with soluble Pb antigens leads to severe PCM in an IL-4 dependent manner
O processo de construção do perfil de competências gerenciais para enfermeiros coordenadores de área hospitalar
Genome-wide association for mapping QTLs linked to protein and oil contents in soybean
Carbono do solo e atributos de fertilidade em resposta à calagem superficial em plantio direto
The genetic architecture of the human cerebral cortex
The cerebral cortex underlies our complex cognitive capabilities, yet little is known about the specific genetic loci that influence human cortical structure. To identify genetic variants that affect cortical structure, we conducted a genome-wide association meta-analysis of brain magnetic resonance imaging data from 51,665 individuals. We analyzed the surface area and average thickness of the whole cortex and 34 regions with known functional specializations. We identified 199 significant loci and found significant enrichment for loci influencing total surface area within regulatory elements that are active during prenatal cortical development, supporting the radial unit hypothesis. Loci that affect regional surface area cluster near genes in Wnt signaling pathways, which influence progenitor expansion and areal identity. Variation in cortical structure is genetically correlated with cognitive function, Parkinson's disease, insomnia, depression, neuroticism, and attention deficit hyperactivity disorder
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