Insulin-Associated Weight Gain in Type 2 Diabetes Is Associated With Increases in Sedentary Behavior.

Contains fulltext : 177371.pdf (publisher's version ) (Closed access)01 september 201

Phylogenetic Analyses of Vitis (Vitaceae) Based on Complete Chloroplast Genome Sequences: Effects of Taxon Sampling and Phylogenetic Methods on Resolving Relationships Among Rosids

Background The Vitaceae (grape) is an economically important family of angiosperms whose phylogenetic placement is currently unresolved. Recent phylogenetic analyses based on one to several genes have suggested several alternative placements of this family, including sister to Caryophyllales, asterids, Saxifragales, Dilleniaceae or to rest of rosids, though support for these different results has been weak. There has been a recent interest in using complete chloroplast genome sequences for resolving phylogenetic relationships among angiosperms. These studies have clarified relationships among several major lineages but they have also emphasized the importance of taxon sampling and the effects of different phylogenetic methods for obtaining accurate phylogenies. We sequenced the complete chloroplast genome of Vitis vinifera and used these data to assess relationships among 27 angiosperms, including nine taxa of rosids. Results The Vitis vinifera chloroplast genome is 160,928 bp in length, including a pair of inverted repeats of 26,358 bp that are separated by small and large single copy regions of 19,065 bp and 89,147 bp, respectively. The gene content and order of Vitis is identical to many other unrearranged angiosperm chloroplast genomes, including tobacco. Phylogenetic analyses using maximum parsimony and maximum likelihood were performed on DNA sequences of 61 protein-coding genes for two datasets with 28 or 29 taxa, including eight or nine taxa from four of the seven currently recognized major clades of rosids. Parsimony and likelihood phylogenies of both data sets provide strong support for the placement of Vitaceae as sister to the remaining rosids. However, the position of the Myrtales and support for the monophyly of the eurosid I clade differs between the two data sets and the two methods of analysis. In parsimony analyses, the inclusion of Gossypium is necessary to obtain trees that support the monophyly of the eurosid I clade. However, maximum likelihood analyses place Cucumis as sister to the Myrtales and therefore do not support the monophyly of the eurosid I clade. Conclusion Phylogenies based on DNA sequences from complete chloroplast genome sequences provide strong support for the position of the Vitaceae as the earliest diverging lineage of rosids. Our phylogenetic analyses support recent assertions that inadequate taxon sampling and incorrect model specification for concatenated multi-gene data sets can mislead phylogenetic inferences when using whole chloroplast genomes for phylogeny reconstruction

The complete chloroplast genome sequence of Gossypium hirsutum: organization and phylogenetic relationships to other angiosperms

BACKGROUND: Cotton (Gossypium hirsutum) is the most important fiber crop grown in 90 countries. In 2004–2005, US farmers planted 79% of the 5.7-million hectares of nuclear transgenic cotton. Unfortunately, genetically modified cotton has the potential to hybridize with other cultivated and wild relatives, resulting in geographical restrictions to cultivation. However, chloroplast genetic engineering offers the possibility of containment because of maternal inheritance of transgenes. The complete chloroplast genome of cotton provides essential information required for genetic engineering. In addition, the sequence data were used to assess phylogenetic relationships among the major clades of rosids using cotton and 25 other completely sequenced angiosperm chloroplast genomes. RESULTS: The complete cotton chloroplast genome is 160,301 bp in length, with 112 unique genes and 19 duplicated genes within the IR, containing a total of 131 genes. There are four ribosomal RNAs, 30 distinct tRNA genes and 17 intron-containing genes. The gene order in cotton is identical to that of tobacco but lacks rpl22 and infA. There are 30 direct and 24 inverted repeats 30 bp or longer with a sequence identity ≥ 90%. Most of the direct repeats are within intergenic spacer regions, introns and a 72 bp-long direct repeat is within the psaA and psaB genes. Comparison of protein coding sequences with expressed sequence tags (ESTs) revealed nucleotide substitutions resulting in amino acid changes in ndhC, rpl23, rpl20, rps3 and clpP. Phylogenetic analysis of a data set including 61 protein-coding genes using both maximum likelihood and maximum parsimony were performed for 28 taxa, including cotton and five other angiosperm chloroplast genomes that were not included in any previous phylogenies. CONCLUSION: Cotton chloroplast genome lacks rpl22 and infA and contains a number of dispersed direct and inverted repeats. RNA editing resulted in amino acid changes with significant impact on their hydropathy. Phylogenetic analysis provides strong support for the position of cotton in the Malvales in the eurosids II clade sister to Arabidopsis in the Brassicales. Furthermore, there is strong support for the placement of the Myrtales sister to the eurosid I clade, although expanded taxon sampling is needed to further test this relationship

Complete Plastid Genome Sequence of Daucus Carota: Implications for Biotechnology and Phylogeny of Angiosperms

Background Carrot (Daucus carota) is a major food crop in the US and worldwide. Its capacity for storage and its lifecycle as a biennial make it an attractive species for the introduction of foreign genes, especially for oral delivery of vaccines and other therapeutic proteins. Until recently efforts to express recombinant proteins in carrot have had limited success in terms of protein accumulation in the edible tap roots. Plastid genetic engineering offers the potential to overcome this limitation, as demonstrated by the accumulation of BADH in chromoplasts of carrot taproots to confer exceedingly high levels of salt resistance. The complete plastid genome of carrot provides essential information required for genetic engineering. Additionally, the sequence data add to the rapidly growing database of plastid genomes for assessing phylogenetic relationships among angiosperms. Results The complete carrot plastid genome is 155,911 bp in length, with 115 unique genes and 21 duplicated genes within the IR. There are four ribosomal RNAs, 30 distinct tRNA genes and 18 intron-containing genes. Repeat analysis reveals 12 direct and 2 inverted repeats ≥ 30 bp with a sequence identity ≥ 90%. Phylogenetic analysis of nucleotide sequences for 61 protein-coding genes using both maximum parsimony (MP) and maximum likelihood (ML) were performed for 29 angiosperms. Phylogenies from both methods provide strong support for the monophyly of several major angiosperm clades, including monocots, eudicots, rosids, asterids, eurosids II, euasterids I, and euasterids II. Conclusion The carrot plastid genome contains a number of dispersed direct and inverted repeats scattered throughout coding and non-coding regions. This is the first sequenced plastid genome of the family Apiaceae and only the second published genome sequence of the species-rich euasterid II clade. Both MP and ML trees provide very strong support (100% bootstrap) for the sister relationship of Daucus with Panax in the euasterid II clade. These results provide the best taxon sampling of complete chloroplast genomes and the strongest support yet for the sister relationship of Caryophyllales to the asterids. The availability of the complete plastid genome sequence should facilitate improved transformation efficiency and foreign gene expression in carrot through utilization of endogenous flanking sequences and regulatory elements

The XMM–NEWTON Ω Project: I. The X-ray luminosity – temperature relation at z>0.4

We describe XMM-Newton Guaranteed Time observations of a sample of eight high redshift (0.45 < z < rvirial) bolometric luminosities, performed β-model fits to the radial surface profiles and made spectral fits to a single temperature isothermal model. We describe data analysis techniques that pay particular attention to background mitigation. We have also estimated temperatures and luminosities for two known clusters (Abell 2246 and RXJ1325.0-3814), and one new high redshift cluste r candidate (XMMU J084701.8 +345117), that were detected o ff-axis. Characterizing the L x − Tx relation as L x = L 6 ( T 6keV ) α , we find L 6 = 15 . 9 + 7 . 6 − 5 . 2 × 1044erg s − 1 and α =2.7 ±0.4 for an Ω Λ = 0 . 0 , Ω M = 1 .0, H0 = 50 km s − 1 Mpc − 1 cosmology at a typical redshift z ∼ 0 .55. Comparing with the low redshift study by Markevitch, 1998, we find α to be in agreement, and assuming L x − Tx to evolve as (1 + z ) A , we find A =0.68 ±0.26 for the same cosmology and A = 1 .52 + 0 .26 − 0 .27 for an Ω Λ = 0 . 7 , Ω M = 0 . 3 cosmology. Our A values are very similar to those found previously by Vikhlinin et al., 2002 using a compilation of Chandra observations of 0 .39 < z < 1 .26 clusters. We conclude that there is now evidence from both XMM-Newton and Chandra for an evolutionary trend in the L x − Tx relation. This evolution is significantly below the level expected from the predictions of the self-similar model for an Ω Λ = 0 . 0 , Ω M = 1 .0, cosmology, but consistent with self-similar model in an Ω Λ = 0 . 7 , Ω M = 0 . 3 cosmology. Our observations lend support to the robustness and completeness of the SHARC and 160SD surveys

Антицитрулінові антитіла в діагностиці артритів у дітей

Віходячи з проведеного дослідження, описаного в роботі, можна дійти висновку, що серед дітей із різними формами артритів тільки 4,8% пацієнтів були серопозитивними за РФ, та 23,8% дітей – за рівнем АЦЦП. Прогнастичне значення підвищеного рівня АЦЦП у дітей із РеА полягає в високій вірогідності розвитку ЮРА, що обумовлює більш ретельне їх спостереження із застосуванням індивідуальних схем профілактичного лікування. При цитуванні документа, використовуйте посилання http://essuir.sumdu.edu.ua/handle/123456789/1128

XMM-Newton discovery of an X-ray filament in Coma

XMM-Newton observations of the outskirts of the Coma cluster of galaxies confirm the existence of a soft X-ray excess claimed previously and show it comes from warm thermal emission. Our data provide a robust estimate of its temperature (~0.2 keV) and oxygen abundance (~0.1 solar). Using a combination of XMM-Newton and ROSAT All-Sky Survey data, we rule out a Galactic origin of the soft X-ray emission. Associating this emission with a 20 Mpc region in front of Coma, seen in the skewness of its galaxy velocity distribution, yields an estimate of the density of the warm gas of ~50 f_baryon rho_critical, where f_baryon is the baryon fraction of the gas and rho_critical is the critical density needed to halt the expansion of the universe. Our measurement of the gas mass associated with the warm emission strongly support its nonvirialized nature, suggesting that we are observing the warm-hot intergalactic medium (WHIM). Our measurements provide a direct estimate of the O, Ne and Fe abundance of the WHIM. Differences with the reported Ne/O ratio for some OVI absorbers hints at a different origin of the OVI absorbers and the Coma filament. We argue that the Coma filament has likely been preheated, but at a substantially lower level compared to what is seen in the outskirts of groups. The thermodynamic state of the gas in the Coma filament reduces the star-formation rate in the embedded spiral galaxies, providing an explanation for the presence of passive spirals observed in this and other clusters.Comment: 9 pages, 5 figures, accepted by A&

White Matter Connectivity Abnormalities in Prediabetes and Type 2 Diabetes:The Maastricht Study

OBJECTIVE: Prediabetes and type 2 diabetes are associated with structural brain abnormalities, often observed in cognitive disorders. Besides visible lesions, (pre)diabetes might also be associated with alterations of the intrinsic organization of the white matter. In this population-based cohort study, the association of prediabetes and type 2 diabetes with white matter network organization was assessed. RESEARCH DESIGN AND METHODS: In the Maastricht Study, a type 2 diabetes-enriched population-based cohort study (1,361 normal glucose metabolism, 348 prediabetes, and 510 type 2 diabetes assessed by oral glucose tolerance test; 52% men; aged 59 ± 8 years), 3 Tesla structural and diffusion MRI was performed. Whole-brain white matter tractography was used to assess the number of connections (node degree) between 94 brain regions and the topology (graph measures). Multivariable linear regression analyses were used to investigate the associations of glucose metabolism status with network measures. Associations were adjusted for age, sex, education, and cardiovascular risk factors. RESULTS: Prediabetes and type 2 diabetes were associated with lower node degree after full adjustment (standardized [st]βPrediabetes = -0.055 [95% CI -0.172, -0.062], stβType2diabetes = -0.256 [-0.379, -0.133], Ptrend < 0.001). Prediabetes was associated with lower local efficiency (stβ = -0.084 [95% CI -0.159, -0.008], P = 0.033) and lower clustering coefficient (stβ = -0.097 [95% CI -0.189, -0.005], P = 0.049), whereas type 2 diabetes was not. Type 2 diabetes was associated with higher communicability (stβ = 0.148 [95% CI 0.042, 0.253], P = 0.008). CONCLUSIONS: These findings indicate that prediabetes and type 2 diabetes are associated with fewer white matter connections and weaker organization of white matter networks. Type 2 diabetes was associated with higher communicability, which was not yet observed in prediabetes and may reflect the use of alternative white matter connections

Star-forming galaxies in SDSS: signs of metallicity evolution

Evolution of galaxies through cosmic time has been widely studied at high redshift, but there are a few studies in this field at lower redshifts. However, low-redshifts studies will provide important clues to the evolution of galaxies, furnishing the required link between local and high-redshift universe. In this work we focus on the metallicity of the gas in spiral galaxies at low redshift looking for signs of chemical evolution. We analyze the metallicity contents of star forming galaxies of similar luminosities at different redshifts, we studied the metallicity of star forming galaxies from SDSS-DR5 (Sloan Digital Sky Survey-Data Release 5), using different redshift intervals from 0.1 to 0.4. We used the public data of SDSS-DR5 processed with the STARLIGHT spectral synthesis code, correcting the fluxes for dust extinction, estimating metallicities using the R23 method, and analyzing the samples with respect to the [NII]6583/[OII]3727 line ratio. From a final sample of 207 galaxies, we find a decrement in 12+log(O/H) corresponding to the redshift interval 0.3 < z < 0.4 of ~0.1 dex with respect to the rest of the sample, which can be interpreted as evidence of the metallicity evolution in low-z galaxies.Comment: 4 pages, 3 figure

RXJ 1821.6+6827: a Cool Cluster at z=0.81 from the ROSAT NEP Survey

We present an analysis of the properties of the cluster of galaxies RXJ 1821.6+6827, or NEP 5281, at a redshift z=0.816+/-0.001. RXJ 1821.6+6827 was discovered during the optical identification of the X-ray sources in the North Ecliptic Pole (NEP) region of the ROSAT All-Sky Survey and it is the highest redshift cluster of galaxies of the NEP survey. We have measured spectroscopic redshifts for twenty cluster galaxies using the Keck-I and the Canada-France-Hawai'i (CFH) telescopes. The value for the cluster velocity dispersion is sigma_V=775(+182,-113) km s-1. The cluster was also observed by XMM-Newton. Both the optical and X-ray data are presented in this paper. The cluster has an unabsorbed X-ray flux in the 2-10 keV energy band of F(2-10 keV)=1.24(+0.16,-0.23) x 10-13 erg cm2 s-1 and a K-corrected luminosity in the same band of L(2-10 keV)=6.32(+76,-0.73)x10^44 h_50^-2 erg s-1 (90% confidence level). The cluster X-ray bolometric luminosity is L(BOL,X)=1.35(+0.08,-0.21)x10^45 h_50^-2 erg s-1. The data do not allow fitting both metal abundance and temperature at the same time. The abundance is unconstrained and can vary in the range 0.28-1.42 Z_sun while the best fit X-ray temperature is T=4.7(+1.2,-0.7) keV. This emission weighted X-ray temperature is a little lower, barely within the uncertainties, than the predicted temperature, T=6.34(+0.13,-0.35) keV, from the L_X-T_X relation of local clusters published in the literature. The optically measured velocitydispersion is consistent with the velocity dispersion expected from the sigma_V-T_X relationship. We also examine the point X-ray source RXJ1821.9+6818, or NEP 5330, located to the south east of the cluster which was identified as a QSO at z=1.692+/-0.008 in the ROSAT NEP survey.Comment: 13 pages including 5 postscript figures and 3 tables. Accepted for publication in the A&A main Journal. A postscript version with Fig. 2 can be downloaded from http://www.ira.cnr.it/~gioia/PUB/publications.htm