Downsizing the Channel Length of Vertical Organic Electrochemical Transistors

Organic electrochemical transistors (OECTs) are promising building blocks for bioelectronic devices such as While the majority of OECTs use simple planar geometry, there is interest in exploring how these devices operate with much shorter channels on the submicron scale. Here, we show a practical route toward the minimization of the channel length of the transistor using traditional photolithography, enabling large-scale utilization. We describe the fabrication of such transistors using two types of conducting polymers. First, commercial solution-processed poly(dioxyethylenethiophene):poly(styrene sulfonate), PEDOT:PSS. Next, we also exploit the short channel length to support easy in situ electropolymerization of poly(dioxyethylenethiophene):tetrabutyl ammonium hexafluorophosphate, PEDOT:PF6. Both variants show different promising features, leading the way in terms of transconductance (gm), with the measured peak gm up to 68 mS for relatively thin (280 nm) channel layers on devices with the channel length of 350 nm and with widths of 50, 100, and 200 m. This result suggests that the use of electropolymerized semiconductors, which can be easily customized, is viable with vertical geometry, as uniform and thin layers can be created. Spin-coated PEDOT:PSS lags behind with the lower values of gm; however, it excels in terms of the speed of the device and also has a comparably lower off current (300 nA), leading to unusually high on/off ratio, with values up to 8.6 × 104. Our approach to vertical gap devices is simple, scalable, and can be extended to other applications where small electrochemical channels are desired

Reconstructing Evolving Tree Structures in Time Lapse Sequences by Enforcing Time-Consistency

We propose a novel approach to reconstructing curvilinear tree structures evolving over time, such as road networks in 2D aerial images or neural structures in 3D microscopy stacks acquired in vivo. To enforce temporal consistency, we simultaneously process all images in a sequence, as opposed to reconstructing structures of interest in each image independently. We formulate the problem as a Quadratic Mixed Integer Program and demonstrate the additional robustness that comes from using all available visual clues at once, instead of working frame by frame. Furthermore, when the linear structures undergo local changes over time, our approach automatically detects them

Direct measurement of oxygen reduction reactions at neurostimulation electrodes

Objective. Electric stimulation delivered by implantable electrodes is a key component of neural engineering. While factors affecting long-term stability, safety, and biocompatibility are a topic of continuous investigation, a widely-accepted principle is that charge injection should be reversible, with no net electrochemical products forming. We want to evaluate oxygen reduction reactions (ORR) occurring at different electrode materials when using established materials and stimulation protocols. Approach. As stimulation electrodes, we have tested platinum, gold, tungsten, nichrome, iridium oxide, titanium, titanium nitride, and poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate). We use cyclic voltammetry and voltage-step amperometry in oxygenated versus inert conditions to establish at which potentials ORR occurs, and the magnitudes of diffusion-limited ORR currents. We also benchmark the areal capacitance of each electrode material. We use amperometric probes (Clark-type electrodes) to quantify the O-2 and H2O2 concentrations in the vicinity of the electrode surface. O-2 and H2O2 concentrations are measured while applying DC current, or various biphasic charge-balanced pulses of amplitude in the range 10-30 mu C cm(-2)/phase. To corroborate experimental measurements, we employ finite element modelling to recreate 3D gradients of O-2 and H2O2. Main results. All electrode materials support ORR and can create hypoxic conditions near the electrode surface. We find that electrode materials differ significantly in their onset potentials for ORR, and in the extent to which they produce H2O2 as a by-product. A key result is that typical charge-balanced biphasic pulse protocols do lead to irreversible ORR. Some electrodes induce severely hypoxic conditions, others additionally produce an accumulation of hydrogen peroxide into the mM range. Significance. Our findings highlight faradaic ORR as a critical consideration for neural interface devices and show that the established biphasic/charge-balanced approach does not prevent irreversible changes in O-2 concentrations. Hypoxia and H2O2 can result in different (electro)physiological consequences

Electrospun PLLA Nanofiber Scaffolds and Their Use in Combination with BMP-2 for Reconstruction of Bone Defects

Introduction Adequate migration and differentiation of mesenchymal stem cells is essential for regeneration of large bone defects. To achieve this, modern graft materials are becoming increasingly important. Among them, electrospun nanofiber scaffolds are a promising approach, because of their high physical porosity and potential to mimic the extracellular matrix (ECM). Materials and Methods The objective of the present study was to examine the impact of electrospun PLLA nanofiber scaffolds on bone formation in vivo, using a critical size rat calvarial defect model. In addition we analyzed whether direct incorporation of bone morphogenetic protein 2 (BMP-2) into nanofibers could enhance the osteoinductivity of the scaffolds. Two critical size calvarial defects (5 mm) were created in the parietal bones of adult male Sprague-Dawley rats. Defects were either (1) left unfilled, or treated with (2) bovine spongiosa, (3) PLLA scaffolds alone or (4) PLLA/BMP-2 scaffolds. Cranial CT-scans were taken at fixed intervals in vivo. Specimens obtained after euthanasia were processed for histology, histomorphometry and immunostaining (Osteocalcin, BMP-2 and Smad5). Results PLLA scaffolds were well colonized with cells after implantation, but only showed marginal ossification. PLLA/BMP-2 scaffolds showed much better bone regeneration and several ossification foci were observed throughout the defect. PLLA/BMP-2 scaffolds also stimulated significantly faster bone regeneration during the first eight weeks compared to bovine spongiosa. However, no significant differences between these two scaffolds could be observed after twelve weeks. Expression of osteogenic marker proteins in PLLA/BMP-2 scaffolds continuously increased throughout the observation period. After twelve weeks osteocalcin, BMP-2 and Smad5 were all significantly higher in the PLLA/BMP-2 group than in all other groups. Conclusion Electrospun PLLA nanofibers facilitate colonization of bone defects, while their use in combination with BMP-2 also increases bone regeneration in vivo and thus combines osteoconductivity of the scaffold with the ability to maintain an adequate osteogenic stimulus

The IceCube Data Acquisition System: Signal Capture, Digitization, and Timestamping

IceCube is a km-scale neutrino observatory under construction at the South Pole with sensors both in the deep ice (InIce) and on the surface (IceTop). The sensors, called Digital Optical Modules (DOMs), detect, digitize and timestamp the signals from optical Cherenkov-radiation photons. The DOM Main Board (MB) data acquisition subsystem is connected to the central DAQ in the IceCube Laboratory (ICL) by a single twisted copper wire-pair and transmits packetized data on demand. Time calibration is maintained throughout the array by regular transmission to the DOMs of precisely timed analog signals, synchronized to a central GPS-disciplined clock. The design goals and consequent features, functional capabilities, and initial performance of the DOM MB, and the operation of a combined array of DOMs as a system, are described here. Experience with the first InIce strings and the IceTop stations indicates that the system design and performance goals have been achieved.Comment: 42 pages, 20 figures, submitted to Nuclear Instruments and Methods

Snow research in Svalbard : an overview

This paper summarizes the most significant snow-related research that has been conducted in Svalbard. Most of the research has been performed during the 1990s and includes investigations of snow distribution, snow-melt, snow pack characteristics, remote sensing of snow and biological studies where snow conditions play an important role. For example, studies have shown regional trends with about 50% higher amounts of snow accumulation at the east coast of Spitsbergen compared to the west coast. Further, the accumulation rates are about twice as high in the south compared to the north. On average, the increase in accumulation with elevation is 97 mm water equivalents per 100 m increase in elevation. Several researchers reported melt rates, which are primarily driven by incoming short-wave radiation, in the range of 10-20 mm/day during spring. Maximum melt rates close to 70 mm/day have been measured. In addition to presenting an overview of research activities, we discuss new, unpublished results in areas where considerable progress is being made. These are i) modelling of snow distribution, ii) modelling of snowmelt runoff and iii) monitoring of snow coverage by satellite imagery. We also identify some weaknesses in current research activities. They are lacks of i) integration between various studies, ii) comparative studies with other Arctic regions, iii) applying local field studies in models that can be used to study larger areas of Svalbard and, finally, iv) using satellite remote sensing data for operational monitoring purposes

Robust Non-Rigid Registration of 2D and 3D Graphs

We present a new approach to matching graphs embedded in R 2 or R 3. Unlike earlier methods, our approach does not rely on the similarity of local appearance features, does not require an initial alignment, can handle partial matches, and can cope with non-linear deformations and topological differences. To handle arbitrary non-linear deformations, we represent them as Gaussian Processes. In the absence of appearance information, we iteratively establish correspondences between graph nodes, update the structure accordingly, and use the current mapping estimate to find the most likely correspondences that will be used in the next iteration. This makes the computation tractable. We demonstrate the effectiveness of our approach first on synthetic cases and then on angiography data, retinal fundus images, and microscopy image stacks acquired at very different resolutions. 1

Robust non-rigid registration of 2D and 3D graphs

We present a new approach to matching graphs embedded in R2 or R3. Unlike earlier methods, our approach does not rely on the similarity of local appearance features, does not require an initial alignment, can handle partial matches, and can cope with non-linear deformations and topological differences. To handle arbitrary non-linear deformations, we represent them as Gaussian Processes. In the absence of appearance information, we iteratively establish correspondences between graph nodes, update the structure accordingly, and use the current mapping estimate to find the most likely correspondences that will be used in the next iteration. This makes the computation tractable. We demonstrate the effectiveness of our approach first on synthetic cases and then on angiography data, retinal fundus images, and microscopy image stacks acquired at very different resolutions.Peer Reviewe

Comparative Study of the Continuous and Batch Thermal Processing of MgB2 Wires

The last stage of the manufacturing process requires complex reactive diffusion formation process of MgB2 in the presence of SiC nanoparticles. Continuous thermal processing was adopted to produce long length MgB2 in situ wires with a homogeneous mixture of micron-sized Mg, nanosized B, as well as SiC dopant powders. This process has enabled the formation of MgB2 superconducting compound in a relatively short time. Traditional superconductor batch processing requires the wire batch to be heat treated in dedicated large furnaces. Additionally, such a batch process requires controllable slow heating-up, dwelling, and cooling down procedures to ensure uniformity of the superconducting properties along the wire length. Such a prolonged reactive diffusion process does require lower dwelling temperature and can potentially prevent full utilization of the doping materials, resulting in less effective pinning centers formation. On the other hand, continuous wire thermal processing enables rapid formation of the doped MgB2 with full utilization of the dopant. Also, in the continuous process, the moving thermal front brings complex dynamics to Mg-B, C-B, Mg-Si interaction during MgB2 formation processes. The manuscript presents a comparative study of the reactive diffusion kinetics, the microstructural formation of the doped MgB2 compound, and their Jc(B, T) characteristics