Evaluation of the occurrence of Salmonella serovars and its Antibiotic Susceptibility in Apparently Healthy domestic animals in rural areas of East Azerbaijan province

Background and Aim: Salmonellosis is one of the more common zoonotic diseases that cause diarrhea and systemic infections. Today, antimicrobial resistances are one of the major health problems in human and veterinary medicine which are more in rural areas. The aim of this study was investigation of presence and antimicrobial resistance profile of Salmonella species in domestic animals which were herd in rural areas (East Azerbaijan province). Material and Methods: In this study a total number of 250 samples were obtained from domestic ruminant (Including 100 cows, 50 sheep, 50 goat and 50 buffalo) in rural areas. The feces and rectal swab specimens cultured in enrichment and selective media and isolates were characterized by biochemical test and stereotyped by O and H antisera. Salmonella isolates were tested for sensitivities to routine antibiotic by method of Kirby-Bauer disc diffusion method. Results and Conclusions: Out of two hundred and fifty samples tested, 15 (6%) were identified as Salmonella strains including S. dublin (7 cases), S. typhimurium (5 cases) and S. enteritidis (3 cases). Many isolates proved multi antibiotic resistance (MAR). In overall, isolating multiple antibiotic resistance Salmonella species at high rates showed apparently healthy ruminant might act as reservoirs and could pose an important public health threat

Comparison of the Photobleaching and Photostability Traits of Alexa Fluor 568- and Fluorescein Isothiocyanate- conjugated Antibody

Objective: Synthetic fluorescent dyes that are conjugated to antibodies are useful tools toprobe molecules. Based on dye chemical structures, their photobleaching and photostabilityindices are quite diverse. It is generally believed that among different fluorescent dyes,Alexa Fluor family has greater photostability than traditional dyes like fluorescein isothiocyanate(FITC) and Cy5. Alexa Fluor 568 is a member of Alexa Fluor family presumed tohave superior photostability and photobleahing profiles than FITC.Materials and Methods: In this experimental study, we conjugated Alexa Fluor 568 andFITC dyes to a mouse anti-human nestin monoclonal antibody (ANM) to acquire their photobleachingprofiles and photostability indices. Then, the fluorophore/antibody ratios werecalculated using a spectrophotometer. The photobleaching profiles and photostability indicesof conjugated antibodies were subsequently studied by immunocytochemistry (ICC).Samples were continuously illuminated and digital images acquired under a fluorescentmicroscope. Data were processed by ImageJ software.Results: Alexa Fluor 568 has a brighter fluorescence and higher photostability thanFITC.Conclusion: Alexa Fluor 568 is a capable dye to use in photostaining techniques and ithas a longer photostability when compared to FITC

Integrated bio- and carbon-isotope stratigraphy of the Upper Cretaceous Gurpi Formation (Iran):A new reference for the eastern Tethys and its implications for large-scale correlation of stage boundaries

29 pagesInternational audienceA high-resolution stratigraphic analysis of the Upper Cretaceous Gurpi Formation has been undertaken in the Shahneshin section (Zagros Basin, Iran). New results on calcareous nannofossils, planktic foraminifers, dinoflagellate cysts and high-resolution carbon and oxygen stable isotopes form the basis of a reference section for the eastern Tethys that spans the upper Coniacian to the late Danian. Carbon-isotope correlation to Gubbio, Italy and the NW German chalk allows for the identification of many isotopic events as well as for the definition of new events in the Campanian and Maastrichtian. Our results allow for a review of the accurate position of the Coniacian/Santonian, Santonian/Campanian, and Campanian/Maastrichtian stage boundaries relative to carbon isotopes and plankton bioevents. The reliability of Coniacian to Maastrichtian planktic foraminifer, dinoflagellate cysts and calcareous nannofossil biohorizons is assessed