Pilot Test of Dosage Effects in HEXORR II for Robotic Hand Movement Therapy in Individuals With Chronic Stroke

Impaired use of the hand in functional tasks remains difficult to overcome in many individuals after a stroke. This often leads to compensation strategies using the less-affected limb, which allows for independence in some aspects of daily activities. However, recovery of hand function remains an important therapeutic goal of many individuals, and is often resistant to conventional therapies. In prior work, we developed HEXORR I, a robotic device that allows practice of finger and thumb movements with robotic assistance. In this study, we describe modifications to the device, now called HEXORR II, and a clinical trial in individuals with chronic stroke. Fifteen individuals with a diagnosis of chronic stroke were randomized to 12 or 24 sessions of robotic therapy. The sessions involved playing several video games using thumb and finger movement. The robot applied assistance to extension movement that was adapted based on task performance. Clinical and motion capture evaluations were performed before and after training and again at a 6-month followup. Fourteen individuals completed the protocol. Fugl-Meyer scores improved significantly at the 6 month time point compared to baseline, indicating reductions in upper extremity impairment. Flexor hypertonia (Modified Ashworth Scale) also decreased significantly due to the intervention. Motion capture found increased finger range of motion and extension ability after the intervention that continued to improve during the followup period. However, there was no change in a functional measure (Action Research Arm Test). At the followup, the high dose group had significant gains in hand displacement during a forward reach task. There were no other significant differences between groups. Future work with HEXORR II should focus on integrating it with functional task practice and incorporating grip and squeezing tasks.Trial Registration:ClinicalTrials.gov, NCT04536987. Registered 3 September 2020 - Retrospectively registered, https://clinicaltrials.gov/ct2/show/record/NCT04536987

Acoustic modelling of exhaust devices with nonconforming finite element meshes and transfer matrices

[EN] Transfer matrices are commonly considered in the numerical modelling of the acoustic behaviour associated with exhaust devices in the breathing system of internal combustion engines, such as catalytic converters, particulate filters, perforated mufflers and charge air coolers. In a multidimensional finite element approach, a transfer matrix provides a relationship between the acoustic fields of the nodes located at both sides of a particular region. This approach can be useful, for example, when one-dimensional propagation takes place within the region substituted by the transfer matrix. As shown in recent investigations, the sound attenuation of catalytic converters can be properly predicted if the monolith is replaced by a plane wave four-pole matrix. The finite element discretization is retained for the inlet/outlet and tapered ducts, where multidimensional acoustic fields can exist. In this case, only plane waves are present within the capillary ducts, and three-dimensional propagation is possible in the rest of the catalyst subcomponents. Also, in the acoustic modelling of perforated mufflers using the finite element method, the central passage can be replaced by a transfer matrix relating the pressure difference between both sides of the perforated surface with the acoustic velocity through the perforations. The approaches in the literature that accommodate transfer matrices and finite element models consider conforming meshes at connecting interfaces, therefore leading to a straightforward evaluation of the coupling integrals. With a view to gaining flexibility during the mesh generation process, it is worth developing a more general procedure. This has to be valid for the connection of acoustic subdomains by transfer matrices when the discretizations are nonconforming at the connecting interfaces. In this work, an integration algorithm similar to those considered in the mortar finite element method, is implemented for nonmatching grids in combination with acoustic transfer matrices. A number of numerical test problems related to some relevant exhaust devices are then presented to assess the accuracy and convergence performance of the proposed procedure.Authors gratefully acknowledge the financial support of Ministerio de Ciencia e Innovacion and the European Regional Development Fund by means of the Projects DPI2007-62635 and DPI2010-15412.Denia, F.; Martínez-Casas, J.; Baeza, L.; Fuenmayor, F. (2012). Acoustic modelling of exhaust devices with nonconforming finite element meshes and transfer matrices. Applied Acoustics. 73(8):713-722. https://doi.org/10.1016/j.apacoust.2012.02.003S71372273

The nutraceutical antihypertensive action of C-phycocyanin in chronic kidney disease is related to the prevention of endothelial dysfunction

C-phycocyanin (CPC) is an antihypertensive that is not still wholly pharmacologically described. The aim of this study was to evaluate whether CPC counteracts endothelial dysfunction as an antihypertensive mechanism in rats with 5/6 nephrectomy (NFx) as a chronic kidney disease (CKD) model. Twenty-four male Wistar rats were divided into four groups: sham control, sham-treated with CPC (100 mg/Kg/d), NFx, and NFx treated with CPC. Blood pressure was measured each week, and renal function evaluated at the end of the treatment. Afterward, animals were euthanized, and their thoracic aortas were analyzed for endothelium functional test, oxidative stress, and NO production. 5/6 Nephrectomy caused hypertension increasing lipid peroxidation and ROS production, overexpression of inducible nitric oxide synthase (iNOS), reduction in the first-line antioxidant enzymes activities, and reduced-glutathione (GSH) with a down-expression of eNOS. The vasomotor response reduced endothelium-dependent vasodilation in aorta segments exposed to acetylcholine and sodium nitroprusside. However, the treatment with CPC prevented hypertension by reducing oxidative stress, NO system disturbance, and endothelial dysfunction. The CPC treatment did not prevent CKD-caused disturbance in the antioxidant enzymes activities. Therefore, CPC exhibited an antihypertensive activity while avoiding endothelial dysfunction

Potential contribution of distant sources to airborne Betula pollen levels in Northeastern Iberian Peninsula

Betula (birch) pollen is one of the most important causes of respiratory allergy in Northern and Central Europe. While birch trees are abundant inCentral, Northern, and Eastern Europe,theyare scarce inthe Mediterranean territories, especially in the Iberian Peninsula (IP), where they grow only in the northern regions and as ornamental trees in urban areas. However, the airborne birch pollen patterns in Catalonia (Northeastern IP) show abrupt high concentrations in areas withusually low local influence.The intensity of the derived health problemscan beincreasedbyoutbreaksdue to long-range pollen transport. The present work evaluates the different potential contributions to Catalonia from the main source regions: Pyrenees, Cantabria, and the forests of France and Central Europe. To this end, we computed the Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) back trajectories of air masses associated with the main Betula pollen peaks occurring simultaneously over different Catalan monitoring stations, and we studied their provenance over a 15-year period. The Vielha aerobiological station on the northern slopes of the Central Pyrenees was used to identify the dates of the pollen season in the Pyrenean region. In order to better understand the role of the Pyrenees, whichis thenearest of thefourbirch forested regions, weclassifiedthepollenpeaksinthe other Catalan stations into three groups based onthe relationship between the peak andthe pollenseason in thePyrenees. Our analysis of back-trajectory residence time, combined with the associated pollen concentration, reveals that two principal routes other than the Pyrenean forest sustain the northerly fluxes that enter Catalonia and carry significant concentrations of Betula pollen. This study has also allowed quantifying the differentiated contributions of the potential sourcePeer ReviewedObjectius de Desenvolupament Sostenible::3 - Salut i BenestarObjectius de Desenvolupament Sostenible::15 - Vida d'Ecosistemes TerrestresPostprint (published version

The see-saw mechanism: neutrino mixing, leptogenesis and lepton flavor violation

The see-saw mechanism to generate small neutrino masses is reviewed. After summarizing our current knowledge about the low energy neutrino mass matrix we consider reconstructing the see-saw mechanism. Low energy neutrino physics is not sufficient to reconstruct see-saw, a feature which we refer to as ``see-saw degeneracy''. Indirect tests of see-saw are leptogenesis and lepton flavor violation in supersymmetric scenarios, which together with neutrino mass and mixing define the framework of see-saw phenomenology. Several examples are given, both phenomenological and GUT-related. Variants of the see-saw mechanism like the type II or triplet see-saw are also discussed. In particular, we compare many general aspects regarding the dependence of LFV on low energy neutrino parameters in the extreme cases of a dominating conventional see-saw term or a dominating triplet term. For instance, the absence of mu -> e gamma or tau -> e gamma in the pure triplet case means that CP is conserved in neutrino oscillations. Scanning models, we also find that among the decays mu -> e gamma, tau -> e gamma and tau -> mu gamma the latter one has the largest branching ratio in (i) SO(10) type I see-saw models and in (ii) scenarios in which the triplet term dominates in the neutrino mass matrix.Comment: 26 pages, 7 figures. Expanded version of talk given at 10th Workshop In High Energy Physics Phenomenology (WHEPP 10), January 2008, Chennai, India. Typos corrected, comments and references adde

Functionalization of Pyrene To Prepare Luminescent Materials—Typical Examples of Synthetic Methodology

Pyrene-based π-conjugated materials are considered to be an ideal organic electro-luminescence material for application in semiconductor devices, such as organic light-emitting diodes (OLEDs), organic field-effect transistors (OFETs) and organic photovoltaics (OPVs), and so forth. However, the great drawback of employing pyrene as an organic luminescence material is the formation of excimer emission, which quenches the efficiency at high concentration or in the solid-state. Thus, in order to obtain highly efficient optical devices, scientists have devoted much effort to tuning the structure of pyrene derivatives in order to realize exploitable properties by employing two strategies, 1) introducing a variety of moieties at the pyrene core, and 2) exploring effective and convenient synthetic strategies to functionalize the pyrene core. Over the past decades, our group has mainly focused on synthetic methodologies for functionalization of the pyrene core; we have found that formylation/acetylation or bromination of pyrene can selectly lead to functionalization at K-region by Lewis acid catalysis. Herein, this Minireview highlights the direct synthetic approaches (such as formylation, bromination, oxidation, and de-tert-butylation reactions, etc.) to functionalize the pyrene in order to advance research on luminescent materials for organic electronic applications. Further, this article demonstrates that the future direction of pyrene chemistry is asymmetric functionalization of pyrene for organic semiconductor applications and highlights some of the classical asymmetric pyrenes, as well as the latest breakthroughs. In addition, the photophysical properties of pyrene-based molecules are briefly reviewed. To give a current overview of the development of pyrene chemistry, the review selectively covers some of the latest reports and concepts from the period covering late 2011 to the present day

Structural and dynamical properties of superfluid helium: a density functional approach

We present a novel density functional for liquid 4He, properly accounting for the static response function and the phonon-roton dispersion in the uniform liquid. The functional is used to study both structural and dynamical properties of superfluid helium in various geometries. The equilibrium properties of the free surface, droplets and films at zero temperature are calculated. Our predictions agree closely to the results of ab initio Monte Carlo calculations, when available. The introduction of a phenomenological velocity dependent interaction, which accounts for backflow effects, is discussed. The spectrum of the elementary excitations of the free surface and films is studied.Comment: 37 pages, REVTeX 3.0, figures on request at [email protected]

Progressive Purkinje Cell Degeneration in tambaleante Mutant Mice Is a Consequence of a Missense Mutation in HERC1 E3 Ubiquitin Ligase

The HERC gene family encodes proteins with two characteristic domains: HECT and RCC1-like. Proteins with HECT domains have been described to function as ubiquitin ligases, and those that contain RCC1-like domains have been reported to function as GTPases regulators. These two activities are essential in a number of important cellular processes such as cell cycle, cell signaling, and membrane trafficking. Mutations affecting these domains have been found associated with retinitis pigmentosa, amyotrophic lateral sclerosis, and cancer. In humans, six HERC genes have been reported which encode two subgroups of HERC proteins: large (HERC1-2) and small (HERC3-6). The giant HERC1 protein was the first to be identified. It has been involved in membrane trafficking and cell proliferation/growth through its interactions with clathrin, M2-pyruvate kinase, and TSC2 proteins. Mutations affecting other members of the HERC family have been found to be associated with sterility and growth retardation. Here, we report the characterization of a recessive mutation named tambaleante, which causes progressive Purkinje cell degeneration leading to severe ataxia with reduced growth and lifespan in homozygous mice aged over two months. We mapped this mutation in mouse chromosome 9 and then performed positional cloning. We found a G⇔A transition at position 1448, causing a Gly to Glu substitution (Gly483Glu) in the highly conserved N-terminal RCC1-like domain of the HERC1 protein. Successful transgenic rescue, with either a mouse BAC containing the normal copy of Herc1 or with the human HERC1 cDNA, validated our findings. Histological and biochemical studies revealed extensive autophagy associated with an increase of the mutant protein level and a decrease of mTOR activity. Our observations concerning this first mutation in the Herc1 gene contribute to the functional annotation of the encoded E3 ubiquitin ligase and underline the crucial and unexpected role of this protein in Purkinje cell physiology

The Myostatin gene: an overview of mechanisms of action and its relevance to livestock animals

Myostatin, also known as Growth Differentiation Factor 8, a member of the Transforming Growth Factor-beta (TGF-β) super-family is a negative regulator of muscle development. Myostatin acts at key points during pre- and post-natal life of amniotes which ultimately determine the overall muscle mass of an animal. Mutations have already demonstrated the impact of attenuating Myostatin activity on muscle development. A number of large animals including cattle, sheep, dogs and humans display the ‘double muscled’ phenotype due to mutations in the Myostatin gene. Here we firstly give an overview of the molecular pathways regulated by Myostatin that control muscle development. Then we describe the natural mutations and their associated phenotypes as well as the physiological influence of altering Myostatin expression in livestock animals (cattle, sheep, goat, horse, pig, rabbit and chicken). Knowledge of null alleles and polymorphisms in the Myostatin gene are of great interest in the animal breeding field and it could be utilized to improve meat production in livestock animals