Thermodynamics of the ATPase Cycle of GlcV, the Nucleotide-Binding Domain of the Glucose ABC Transporter of Sulfolobus solfataricus

ATP-binding cassette transporters drive the transport of substrates across the membrane by the hydrolysis of ATP. They typically have a conserved domain structure with two membrane-spanning domains that form the transport channel and two cytosolic nucleotide-binding domains (NBDs) that energize the transport reaction. Binding of ATP to the NBD monomer results in formation of a NBD dimer. Hydrolysis of the ATP drives the dissociation of the dimer. The thermodynamics of distinct steps in the ATPase cycle of GlcV, the NBD of the glucose ABC transporter of the extreme thermoacidophile Sulfolobus solfataricus, were studied by isothermal titration calorimetry using the wild-type protein and two mutants, which are arrested at different steps in the ATP hydrolytic cycle. The G144A mutant is unable to dimerize, while the E166A mutant is defective in dimer dissociation. The ATP, ADP, and AMP-PNP binding affinities, stoichiometries, and enthalpies of binding were determined at different temperatures. From these data, the thermodynamic parameters of nucleotide binding, NBD dimerization, and ATP hydrolysis were calculated. The data demonstrate that the ATP hydrolysis cycle of isolated NBDs consists of consecutive steps where only the final step of ADP release is energetically unfavorable.

Finding robust investments for the Dutch gas distribution infrastructure in 2050 by a scenario study

In the changing Dutch energy market, the ageing gas distribution grid needs investments. There is, however, a large uncertainty regarding certain aspects that affect the future role of this gas distribution grid, such as the eventual share of biomethane in the gas mix, whether power-to-gas will take-off and the expected lifetime of the grid. Hence, it is currently unclear what investments need to be made for the gas distribution infrastructure in order to cope with future changes. To find out how the future may unfold for the Dutch gas distribution infrastructure in 2050, four scenarios have been developed. Two key forces – a key force is a factor that has a large impact on the gas distribution grid but great uncertainty exists regarding its outcome – were chosen, namely “perceived energy resource scarcity” and “willingness and ability to reduce greenhouse gas emissions”. Each scenario sketches the future Dutch gas infrastructure by defining, among others, the extent to which biomass will be used to produce biomethane, whether fossil fuel is allowed to be burned locally, and what types of gas will flow through the gas grid. With these scenarios at our disposal, in the next research step, we will establish the impact of the scenarios on the gas distribution grid – i.e. what the layout will be and the corresponding costs – and the accompanying biomethane infrastructure. For this, 3 typical locations were chosen: a rural region, an urban region, and an intermediate region. Through a multi-objective optimization – with maximizing net present value, maximizing biomethane production, and maximizing CO2 emission reduction as objectives – the possible layout of the gas distribution grid and the biomethane infrastructure will be determined for each region. The study’s aim is to find similarities in investments among the different layouts, to come to robust investments for the gas distribution infrastructure

Gravitomagnetism and the Clock Effect

The main theoretical aspects of gravitomagnetism are reviewed. It is shown that the gravitomagnetic precession of a gyroscope is intimately connected with the special temporal structure around a rotating mass that is revealed by the gravitomagnetic clock effect. This remarkable effect, which involves the difference in the proper periods of a standard clock in prograde and retrograde circular geodesic orbits around a rotating mass, is discussed in detail. The implications of this effect for the notion of ``inertial dragging'' in the general theory of relativity are presented. The theory of the clock effect is developed within the PPN framework and the possibility of measuring it via spaceborne clocks is examined.Comment: 27 pages, LaTeX, submitted to Proc. Bad Honnef Meeting on: GYROS, CLOCKS, AND INTERFEROMETERS: TESTING GENERAL RELATIVITY IN SPACE (22 - 27 August 1999; Bad Honnef, Germany

Possibilities and challenges of the potato genome sequence

This paper describes the progress that has been made since the draft genome sequence of potato has been obtained and the analyses that need to be done to make further progress. Although sequencing has become less expensive and read lengths have increased, making optimal use of the information obtained is still difficult, certainly in the tetraploid potato crop. Major challenges in potato genomics are standardized genome assembly and haplotype analysis. Sequencing methods need to be improved further to achieve precision breeding. With the current new generation sequencing technology, the focus in potato breeding will shift from phenotype improvement to genotype improvement. In this respect, it is essential to realize that different alleles of the same gene can lead to different phenotypes depending on the genetic background and that there is significant epistatic interaction between different alleles. Genome-wide association studies will gain statistical power when binary single nucleotide polymorphism (SNP) data can be replaced with multi-allelic haplotype data. Binary SNP can be distributed across the many different alleles per locus or may be haplotype-specific, and potentially tag specific alleles which clearly differ in their contribution to a certain trait value. Assembling reads from the same linkage phase proved to allow constructing sufficiently long haplotype tracts to ensure their uniqueness. Combining large phenotyping data sets with modern approaches to sequencing and haplotype analysis and proper software will allow the efficiency of potato breeding to increase

Search for the Flavor-Changing Neutral Current Decay D0→μ+μ−D^0 \to \mu^+\mu^- with the HERA-B Detector

We report on a search for the flavor-changing neutral current decay $D^0 \to \mu^+\mu^-$ using $50 \times 10^6$ events recorded with a dimuon trigger in interactions of 920 GeV protons with nuclei by the HERA-B experiment. We find no evidence for such decays and set a 90% confidence level upper limit on the branching fraction $Br(D^0 \to \mu^+\mu^-) <2.0 \times 10^{-6}$.Comment: 17 pages, 4 figures (of which 1 double), paper to be submitted to Physics Letters

Measurement of the J/Psi Production Cross Section in 920 GeV/c Fixed-Target Proton-Nucleus Interactions

The mid-rapidity (dsigma_(pN)/dy at y=0) and total sigma_(pN) production cross sections of J/Psi mesons are measured in proton-nucleus interactions. Data collected by the HERA-B experiment in interactions of 920 GeV/c protons with carbon, titanium and tungsten targets are used for this analysis. The J/Psi mesons are reconstructed by their decay into lepton pairs. The total production cross section obtained is sigma_(pN)(J/Psi) = 663 +- 74 +- 46 nb/nucleon. In addition, our result is compared with previous measurements

Hadron Energy Reconstruction for the ATLAS Calorimetry in the Framework of the Non-parametrical Method

This paper discusses hadron energy reconstruction for the ATLAS barrel prototype combined calorimeter (consisting of a lead-liquid argon electromagnetic part and an iron-scintillator hadronic part) in the framework of the non-parametrical method. The non-parametrical method utilizes only the known $e/h$ ratios and the electron calibration constants and does not require the determination of any parameters by a minimization technique. Thus, this technique lends itself to an easy use in a first level trigger. The reconstructed mean values of the hadron energies are within $\pm 1$ of the true values and the fractional energy resolution is $[(58\pm3)/\sqrt{E}+(2.5\pm0.3)$. The value of the $e/h$ ratio obtained for the electromagnetic compartment of the combined calorimeter is $1.74\pm0.04$ and agrees with the prediction that $e/h > 1.7$ for this electromagnetic calorimeter. Results of a study of the longitudinal hadronic shower development are also presented. The data have been taken in the H8 beam line of the CERN SPS using pions of energies from 10 to 300 GeV.Comment: 33 pages, 13 figures, Will be published in NIM