Comparison of vapour pressure deficit patterns during cucumber cultivation in a traditional high PE tunnel greenhouse and a tunnel greenhouse equipped with a heat accumulator

Plant productivity in protected cultivation is highly influenced by air temperature and humidity. The conditions relating to the moisture content of the air in protected plant cultivation are preferably defined by vapour pressure deficit (VPD), which describes the difference between the maximal and actual water vapour pressure (kPa). VPD is widely used as the parameter describing the climate conditions favourable for the development of fungal diseases and for highlighting conditions unfavourable for plant development. In protected cultivation, both the air temperature and the humidity are influenced by heating systems, and one such system is a heat accumulator, which may store the excessive heat produced during the day by converting the solar energy inside the plastic tunnel, and using it when plant heating is required. The tunnel equipped with a heat accumulator maintained an optimal level of humidity for a longer period, and significantly reduced the time of excessive air humidity. The longest time with an optimal VPD was recorded in August in a tunnel with an accumulator – 30.5% of total time vs. 22.3% of time for control tunnel. The highest difference of total time where the VPD was too low (below 0.2 kPa) was recorded in July – 12.4% of time in a tunnel with an accumulator vs. 39.1% of time for control tunnel. The highest difference of total time with an excessive VPD (over 1.4 kPa) was recorded in May – 12.1% of time in a tunnel with an accumulator vs. 17.9% of time for control tunnel. However, a situation beneficial for plant growth occurred every month during the investigated season

Estimates of energy consumption by building type and end use at U.S. Army installations

This report discusses the use of LBNL`s End-use Disaggregation Alogrithm (EDA) to 12 US Army installations nationwide in order to obtain annual estimates of electricity use for all major building types and end uses. The building types include barrack, dining hall, gymnasium, administration, vehicle maintenance, hospital, residential, warehouse, and misc. Up to 8 electric end uses for each type were considered: space cooling, ventilation (air handling units, fans, chilled and hot water pumps), cooking, misc./plugs, refrigeration, exterior and interior lighting, and process loads. Through building simulations, we also obtained estimates of natural gas space heating energy use. Average electricity use for these 12 installations and Fort Hood are: HVAC, misc., and indoor lighting end uses consumed the most electricity (28, 27, and 26% of total[3.8, 3.5, and 3.3 kWh/ft{sup 2}]). Refrigeration, street lighting, exterior lighting, and cooking consumed 7, 7, 3, and 2% of total (0.9, 0.9, 0.4, and 0.3 kWh/ft{sup 2}

Phase-amplitude coupled persistent theta and gamma oscillations in rat primary motor cortex in vitro

In vivo, theta (4-7 Hz) and gamma (30-80 Hz) neuronal network oscillations are known to coexist and display phase-amplitude coupling (PAC). However, in vitro, these oscillations have for many years been studied in isolation. Using an improved brain slice preparation technique we have, using co-application of carbachol (10 μM) and kainic acid (150 nM), elicited simultaneous theta (6.6 ± 0.1 Hz) and gamma (36.6 ± 0.4 Hz) oscillations in rodent primary motor cortex (M1). Each oscillation showed greatest power in layer V. Using a variety of time series analyses we detected significant cross-frequency coupling 74% of slice preparations. Differences were observed in the pharmacological profile of each oscillation. Thus, gamma oscillations were reduced by the GABAA receptor antagonists, gabazine (250 nM and 2 μM), and picrotoxin (50 μM) and augmented by AMPA receptor antagonism with SYM2206 (20 μM). In contrast, theta oscillatory power was increased by gabazine, picrotoxin and SYM2206. GABAB receptor blockade with CGP55845 (5 μM) increased both theta and gamma power, and similar effects were seen with diazepam, zolpidem, MK801 and a series of metabotropic glutamate receptor antagonists. Oscillatory activity at both frequencies was reduced by the gap junction blocker carbenoxolone (200 μM) and by atropine (5 μM). These data show theta and gamma oscillations in layer V of rat M1 in vitro are cross-frequency coupled, and are mechanistically distinct. The development of an in vitro model of phase-amplitude coupled oscillations will facilitate further mechanistic investigation of the generation and modulation of coupled activity in mammalian cortex

Ferritin immobilization on patterned poly(2-hydroxyethyl methacrylate) brushes on silicon surfaces from colloid system

In this paper, we describe a graft polymerization/solvent immersion method for generating poly(2-hydroxyethyl methacrylate) (PHEMA) brushes in various patterns. We used a novel fabrication process, involving very-large-scale integration and oxygen plasma treatment, to generate well-defined patterns of polymerized PHEMA on patterned Si(100) surfaces. We observed brush- and mushroom-like regions for the PHEMA brushes, with various pattern resolutions, after immersing wafers presenting lines of these polymers in MeOH and n-hexane, respectively. The interaction between PHEMA and ferritin protein sheaths in MeOH and n-hexane (good and poor solvent for PHEMA, respectively) was used to capture and release ferritins from fluidic system. The “tentacles” behaver for PHEMA brushes was found through various solvents in fluidic system. Using high-resolution scanning electron microscopy, we observed patterned ferritin Fe cores on the Si surface after pyrolysis of the patterned PHEMA brushes and ferritin protein sheaths, which verify the “tentacles” behaver for PHEMA brushes

Dopamine acting at D1-like, D2-like and α1-adrenergic receptors differentially modulates theta and gamma oscillatory activity in primary motor cortex

The loss of dopamine (DA) in Parkinson’s is accompanied by the emergence of exaggerated theta and beta frequency neuronal oscillatory activity in the primary motor cortex (M1) and basal ganglia. DA replacement therapy or deep brain stimulation reduces the power of these oscillations and this is coincident with an improvement in motor performance implying a causal relationship. Here we provide in vitro evidence for the differential modulation of theta and gamma activity in M1 by DA acting at receptors exhibiting conventional and non-conventional DA pharmacology. Recording local field potentials in deep layer V of rat M1, co-application of carbachol (CCh, 5 μM) and kainic acid (KA, 150 nM) elicited simultaneous oscillations at a frequency of 6.49 ± 0.18 Hz (theta, n = 84) and 34.97 ± 0.39 Hz (gamma, n = 84). Bath application of DA resulted in a decrease in gamma power with no change in theta power. However, application of either the D1-like receptor agonist SKF38393 or the D2-like agonist quinpirole increased the power of both theta and gamma suggesting that the DA-mediated inhibition of oscillatory power is by action at other sites other than classical DA receptors. Application of amphetamine, which promotes endogenous amine neurotransmitter release, or the adrenergic α1-selective agonist phenylephrine mimicked the action of DA and reduced gamma power, a result unaffected by prior co-application of D1 and D2 receptor antagonists SCH23390 and sulpiride. Finally, application of the α1-adrenergic receptor antagonist prazosin blocked the action of DA on gamma power suggestive of interaction between α1 and DA receptors. These results show that DA mediates complex actions acting at dopamine D1-like and D2-like receptors, α1 adrenergic receptors and possibly DA/α1 heteromultimeric receptors to differentially modulate theta and gamma activity in M1

Severe Aplastic Anemia and PNH

Severe aplastic anemia (SAA) is an autoimmune disorder (AID) due to the attack of autoreactive cytotoxic T lymphocytes to the hematopoietic component of the bone marrow

Augmented reality as a tool supporting assessment and decision-making processes in architecture and spatial planning

Artykuł przedstawia na przykładach wdrożeń metodę i zastosowania technologii rozszerzonej rzeczywistości jako narzędzia wspomagającego podejmowanie decyzji lokalizacyjnych obiektów architektonicznych oraz doboru formy w kontekście zastanej kompozycji. Wskazuje wady i określa zalety zastosowanych metod. Opisuje eksperyment badający przydatność autorskich rozwiązań systemowych eliminujących aktualnie występujące ograniczania, które mogą mieć wpływ na efektywność pracy i jakość osiąganych rozwiązań przestrzennych.The article presents examples of implementation of the augmented reality technology as a tool supporting making decisions on location of architectural objects and selection of form thereof in the context of the existing composition of the site. It points out to the deficiencies as well as to the merits of the applied methods. It describes an experiment examining the usefulness of the author’s solutions eliminating the current limitations of the technology, which can affect the effectiveness of work and the quality of the achieved spatial solutions