Experiments on standing bubbles in a vertical pipe

We present a series of laboratory experiments in which a steady stream of air is supplied through a small hole in the wall of a vertical pipe of rectangular cross-section down which there is a steady flux of water. For a range of liquid flow rates, the air forms a steady standing bubble whose nose is attached to the point of air supply. The steady bubble sheds a flux of much smaller air bubbles at its base, located downstream of the air injection point. The minimum liquid speed for which steady standing bubbles develop occurs at a particular Froude number of the liquid flow, Frd = U/$/sqrt{\it gd}$ = 0.38, where U is the upstream speed, g the acceleration due to gravity and d the width of the cell. These trapped bubbles are distinct from the freely rising Taylor bubble, in that the Froude number at the nose is variable. Also, on a length scale greater than that influenced by surface tension, we find that the bubble nose asymptotes to a cusp-like shape, with an angle that decreases with Frd. We show that numerical solutions of the potential flow equations replicate the bubble shape and angle of the cusp, which appear independent of the gas flux. We also find that there is a minimum gas flux for which these standing bubbles develop. As the gas flux decreases below this threshold, the standing bubbles become unstable and, instead, a much shorter oscillating bubble develops. This produces a wake which has similarities with that formed downstream of a cylinder in a confined channel, but which also carries bubbles downstream. We also find that with sufficiently small gas flux, no bubble develops. For liquid flow rates smaller than the critical value, Frd < 0.38, we find that the bubbles become unstable and detach from the injection point and rise up the tube

The maize root stem cell niche: a partnership between two sister cell populations

Using transcript profile analysis, we explored the nature of the stem cell niche in roots of maize (Zea mays). Toward assessing a role for specific genes in the establishment and maintenance of the niche, we perturbed the niche and simultaneously monitored the spatial expression patterns of genes hypothesized as essential. Our results allow us to quantify and localize gene activities to specific portions of the niche: to the quiescent center (QC) or the proximal meristem (PM), or to both. The data point to molecular, biochemical and physiological processes associated with the specification and maintenance of the niche, and include reduced expression of metabolism-, redox- and certain cell cycle-associated transcripts in the QC, enrichment of auxin-associated transcripts within the entire niche, controls for the state of differentiation of QC cells, a role for cytokinins specifically in the PM portion of the niche, processes (repair machinery) for maintaining DNA integrity and a role for gene silencing in niche stabilization. To provide additional support for the hypothesized roles of the above-mentioned and other transcripts in niche specification, we overexpressed, in Arabidopsis, homologs of representative genes (eight) identified as highly enriched or reduced in the maize root QC. We conclude that the coordinated changes in expression of auxin-, redox-, cell cycle- and metabolism-associated genes suggest the linkage of gene networks at the level of transcription, thereby providing additional insights into events likely associated with root stem cell niche establishment and maintenance

The bHLH transcription factor SPATULA enables cytokinin signaling, and both activate auxin biosynthesis and transport genes at the medial domain of the gynoecium

[EN] Fruits and seeds are the major food source on earth. Both derive from the gynoecium and, therefore, it is crucial to understand the mechanisms that guide the development of this organ of angiosperm species. In Arabidopsis, the gynoecium is composed of two congenitally fused carpels, where two domains: medial and lateral, can be distinguished. The medial domain includes the carpel margin meristem (CMM) that is key for the production of the internal tissues involved in fertilization, such as septum, ovules, and transmitting tract. Interestingly, the medial domain shows a high cytokinin signaling output, in contrast to the lateral domain, where it is hardly detected. While it is known that cytokinin provides meristematic properties, understanding on the mechanisms that underlie the cytokinin signaling pattern in the young gynoecium is lacking. Moreover, in other tissues, the cytokinin pathway is often connected to the auxin pathway, but we also lack knowledge about these connections in the young gynoecium. Our results reveal that cytokinin signaling, that can provide meristematic properties required for CMM activity and growth, is enabled by the transcription factor SPATULA (SPT) in the medial domain. Meanwhile, cytokinin signaling is confined to the medial domain by the cytokinin response repressor ARABIDOPSIS HISTIDINE PHOSPHOTRANSFERASE 6 (AHP6), and perhaps by ARR16 (a type-A ARR) as well, both present in the lateral domains (presumptive valves) of the developing gynoecia. Moreover, SPT and cytokinin, probably together, promote the expression of the auxin biosynthetic gene TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1 (TAA1) and the gene encoding the auxin efflux transporter PIN-FORMED 3 (PIN3), likely creating auxin drainage important for gynoecium growth. This study provides novel insights in the spatiotemporal determination of the cytokinin signaling pattern and its connection to the auxin pathway in the young gynoecium.IRO, VMZM, HHU and PLS were supported by the Mexican National Council of Science and Technology (CONACyT) with a PhD fellowship (210085, 210100, 243380 and 219883, respectively). Work in the SDF laboratory was financed by the CONACyT grants CB-2012-177739, FC-2015-2/1061, and INFR-2015-253504, and NMM by the CONACyT grant CB-2011-165986. SDF, CF and LC acknowledge the support of the European Union FP7-PEOPLE-2009-IRSES project EVOCODE (grant no. 247587) and H2020-MSCARISE-2015 project ExpoSEED (grant no. 691109). SDF also acknowledges the Marine Biological Laboratory (MBL) in Woods Hole for a scholarship for the Gene Regulatory Networks for Development Course 2015 (GERN2015). IE acknowledges the International European Fellowship-METMADS project and the Universita degli Studi di Milano (RTD-A; 2016). Research in the laboratory of MFY was funded by NSF (grant IOS-1121055), NIH (grant 1R01GM112976-01A1) and the Paul D. Saltman Endowed Chair in Science Education (MFY). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Reyes Olalde, J.; Zuñiga, V.; Serwatowska, J.; Chávez Montes, R.; Lozano-Sotomayor, P.; Herrera-Ubaldo, H.; Gonzalez Aguilera, K.... (2017). The bHLH transcription factor SPATULA enables cytokinin signaling, and both activate auxin biosynthesis and transport genes at the medial domain of the gynoecium. PLoS Genetics. 13(4):1-31. https://doi.org/10.1371/journal.pgen.1006726S131134Reyes-Olalde, J. I., Zuñiga-Mayo, V. M., Chávez Montes, R. A., Marsch-Martínez, N., & de Folter, S. (2013). Inside the gynoecium: at the carpel margin. Trends in Plant Science, 18(11), 644-655. doi:10.1016/j.tplants.2013.08.002Alvarez-Buylla, E. 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Current Opinion in Plant Biology, 11(1), 42-48. doi:10.1016/j.pbi.2007.10.009Schuster, C., Gaillochet, C., Medzihradszky, A., Busch, W., Daum, G., Krebs, M., … Lohmann, J. U. (2014). A Regulatory Framework for Shoot Stem Cell Co

Genome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch

Silver birch (Betula pendula) is a pioneer boreal tree that can be induced to flower within 1 year. Its rapid life cycle, small (440-Mb) genome, and advanced germplasm resources make birch an attractive model for forest biotechnology. We assembled and chromosomally anchored the nuclear genome of an inbred B. pendula individual. Gene duplicates from the paleohexaploid event were enriched for transcriptional regulation, whereas tandem duplicates were overrepresented by environmental responses. Population resequencing of 80 individuals showed effective population size crashes at major points of climatic upheaval. Selective sweeps were enriched among polyploid duplicates encoding key developmental and physiological triggering functions, suggesting that local adaptation has tuned the timing of and cross-talk between fundamental plant processes. Variation around the tightly-linked light response genes PHYC and FRS10 correlated with latitude and longitude and temperature, and with precipitation for PHYC. Similar associations characterized the growth-promoting cytokinin response regulator ARR1, and the wood development genes KAK and MED5A.Peer reviewe

Plant vascular development: from early specification to differentiation.

Vascular tissues in plants are crucial to provide physical support and to transport water, sugars and hormones and other small signalling molecules throughout the plant. Recent genetic and molecular studies have identified interconnections among some of the major signalling networks that regulate plant vascular development. Using Arabidopsis thaliana as a model system, these studies enable the description of vascular development from the earliest tissue specification events during embryogenesis to the differentiation of phloem and xylem tissues. Moreover, we propose a model for how oriented cell divisions give rise to a three-dimensional vascular bundle within the root meristem

Experiments on standing bubbles in a vertical pipe

We present a series of laboratory experiments in which a steady stream of air is supplied through a small hole in the wall of a vertical pipe of rectangular cross-section down which there is a steady flux of water. For a range of liquid flow rates, the air forms a steady standing bubble whose nose is attached to the point of air supply. The steady bubble sheds a flux of much smaller air bubbles at its base, located downstream of the air injection point. The minimum liquid speed for which steady standing bubbles develop occurs at a particular Froude number of the liquid flow, Frd = U/ = 0.38, where U is the upstream speed, g the acceleration due to gravity and d the width of the cell. These trapped bubbles are distinct from the freely rising Taylor bubble, in that the Froude number at the nose is variable. Also, on a length scale greater than that influenced by surface tension, we find that the bubble nose asymptotes to a cusp-like shape, with an angle that decreases with Frd. We show that numerical solutions of the potential flow equations replicate the bubble shape and angle of the cusp, which appear independent of the gas flux. We also find that there is a minimum gas flux for which these standing bubbles develop. As the gas flux decreases below this threshold, the standing bubbles become unstable and, instead, a much shorter oscillating bubble develops. This produces a wake which has similarities with that formed downstream of a cylinder in a confined channel, but which also carries bubbles downstream. We also find that with sufficiently small gas flux, no bubble develops. For liquid flow rates smaller than the critical value, Frd < 0.38, we find that the bubbles become unstable and detach from the injection point and rise up the tube

A small cog in a large wheel: crucial role of miRNAs in root apical meristem patterning

In both animal and plants, establishment of body axes is fundamental for proper organ development. Plant roots show two main developmental axes: the proximo-distal axis, which spans from the hypocotyl-root junction to the root tip, and the radial axis, which traverses from the vascular tissue to the epidermis.Root axes are determined in the root meristem. The root meristem occupies the tip of the root and contains self-renewing stem cells, which continuously produce new root cells. An intricate network of signalling pathways regulates meristem function and patterning to ensure proper root development and growth. In the last decade, microRNAs (miRNAs), 20-21 nucleotide-long molecules with morphogenetic activity, emerged as central regulators of root cell patterning. Their activity intersects with master regulators of meristematic activity, including phytohormones. In this review we discuss the latest findings about miRNAs activity and their interaction with other molecular networks in the formation of root meristem axes. Furthermore, we describe how these small molecules allow root growth to adapt to changes in the environment, while maintaining a correct patterning

EXPERIMENTAL RESULTS ON A SHARP DOUBLE CONE IN A MACH 9 NONEQUILIBRIUM FLOW

The results of a new experimental campaign on a sharp double cone model placed within Alta’s HEAT pulsed hypersonic tunnel in a Mach 9 non-equilibrium air flow are presented. The model geometry has cone semiaperture angles of 25 and 55 degrees, in order to allow the comparison with other literature experiments and simulations; the maximum diameter is of 70 mm. The model is instrumented on one side with 10 fast pressure sensors and 7 thermocouples, while the other side is left untouched in order to perform fast infrared thermography. The nozzle was realized and characterised in detail in order to provide good quality data regarding the free stream for the numerical reconstruction. The results allowed to compare surface pressure, temperature and heat flux as well as the extension of the recirculation zone with previous literature result