Influence of acoustic cavitation on the controlled ultrasonic dispersion of carbon nanotubes.

Ultrasonication is the most widely used technique for the dispersion of a range of nanomaterials, but the intrinsic mechanism which leads to stable solutions is poorly understood with procedures quoted in the literature typically specifying only extrinsic parameters such as nominal electrical input power and exposure time. Here we present new insights into the dispersion mechanism of a representative nanomaterial, single-walled carbon nanotubes (SW-CNTs), using a novel up-scalable sonoreactor and an in situ technique for the measurement of acoustic cavitation activity during ultrasonication. We distinguish between stable cavitation, which leads to chemical modification of the surface of the CNTs, and inertial cavitation, which favors CNT exfoliation and length reduction. Efficient dispersion of CNTs in aqueous solution is found to be dominated by mechanical forces generated via inertial cavitation, which in turn depends critically on surfactant concentration. This study highlights that careful measurement and control of cavitation rather than blind application of input power is essential in the large volume production of nanomaterial dispersions with tailored properties

Hollow silica capsules for amphiphilic transport and sustained delivery of antibiotic and anticancer drugs

Hollow mesoporous silica capsules HMSC are potential drug transport vehicles due to their biocompatibility, high loading capacity and sufficient stability in biological milieu. Herein, we report the synthesis of ellipsoid shaped HMSC aspect ratio amp; 8764;2 performed using hematite particles as solid templates that were coated with a conformal silica shell through cross condensation reactions. For obtaining hollow silica capsules, the iron oxide core was removed by acidic leaching. Gas sorption studies on HMSC revealed mesoscopic pores main pore width amp; 8764;38 and a high surface area of 308.8 m2 g amp; 8722;1. Cell uptake of dye labeled HMSC was confirmed by incubating them with human cervical cancer HeLa cells and analyzing the internalization through confocal microscopy. The amphiphilic nature of HMSC for drug delivery applications was tested by loading antibiotic ciprofloxacin and anticancer curcumin compounds as model drugs for hydrophilic and hydrophobic therapeutics, respectively. The versatility of HMSC in transporting hydrophilic as well as hydrophobic drugs and a pH dependent drug release over several days under physiological conditions was demonstrated in both cases by UV vis spectroscopy. Ciprofloxacin loaded HMSC were additionally evaluated towards Gram negative E. coli bacteria and demonstrated their efficacy even at low concentrations 10 amp; 956;g ml amp; 8722;1 in inhibiting complete bacterial growth over 18 hour

Targeting CD22 Reprograms B-Cells and Reverses Autoimmune Diabetes

OBJECTIVES—To investigate a B-cell–depleting strategy to reverse diabetes in naïve NOD mice

The Planetary Fourier Spectrometer (PFS) onboard the European Mars Express mission

International audience; The Planetary Fourier Spectrometer (PFS) for the Mars Express mission is an infrared spectrometer optimised for atmospheric studies. This instrument has a short wave (SW) channel that covers the spectral range from 1700 to 8200.0cm-1 (1.2- 5.5mum) and a long-wave (LW) channel that covers 250- 1700cm-1 (5.5- 45mum). Both channels have a uniform spectral resolution of 1.3cm-1. The instrument field of view FOV is about 1.6o (FWHM) for the Short Wavelength channel (SW) and 2.8o (FWHM) for the Long Wavelength channel (LW) which corresponds to a spatial resolution of 7 and 12 km when Mars is observed from an height of 250 km. PFS can provide unique data necessary to improve our knowledge not only of the atmosphere properties but also about mineralogical composition of the surface and the surface-atmosphere interaction. The SW channel uses a PbSe detector cooled to 200-220 K while the LW channel is based on a pyroelectric ( LiTaO3) detector working at room temperature. The intensity of the interferogram is measured every 150 nm of physical mirrors displacement, corresponding to 600 nm optical path difference, by using a laser diode monochromatic light interferogram (a sine wave), whose zero crossings control the double pendulum motion. PFS works primarily around the pericentre of the orbit, only occasionally observing Mars from large distances. Each measurements take 4 s, with a repetition time of 8.5 s. By working roughly 0.6 h around pericentre, a total of 330 measurements per orbit will be acquired 270 looking at Mars and 60 for calibrations. PFS is able to take measurements at all local times, facilitating the retrieval of surface temperatures and atmospheric vertical temperature profiles on both the day and the night side

Treatment of substance abuse in dual diagnosis

Interventions for substance use–related problems are limited for individuals with intellectual disability (ID). This is problematic, as the lack of interventions can lead to substance use initiation, progression of substance use into substance use disorder, poorer outcomes of treatment, and stigmatization of individuals with dual diagnosis. Additionally, staff who work with individuals with ID and addiction treatment lack resources to effectively help substance use in individuals with ID. Nevertheless, there has been an increase in studies assessing the feasibility and outcomes of interventions for substance use and abuse in individuals with ID. This chapter reviews psychological and pharmacological interventions for individuals with dual diagnosis of substance abuse and ID

Mathematical Modelling of Cell-Fate Decision in Response to Death Receptor Engagement

Cytokines such as TNF and FASL can trigger death or survival depending on cell lines and cellular conditions. The mechanistic details of how a cell chooses among these cell fates are still unclear. The understanding of these processes is important since they are altered in many diseases, including cancer and AIDS. Using a discrete modelling formalism, we present a mathematical model of cell fate decision recapitulating and integrating the most consistent facts extracted from the literature. This model provides a generic high-level view of the interplays between NFκB pro-survival pathway, RIP1-dependent necrosis, and the apoptosis pathway in response to death receptor-mediated signals. Wild type simulations demonstrate robust segregation of cellular responses to receptor engagement. Model simulations recapitulate documented phenotypes of protein knockdowns and enable the prediction of the effects of novel knockdowns. In silico experiments simulate the outcomes following ligand removal at different stages, and suggest experimental approaches to further validate and specialise the model for particular cell types. We also propose a reduced conceptual model implementing the logic of the decision process. This analysis gives specific predictions regarding cross-talks between the three pathways, as well as the transient role of RIP1 protein in necrosis, and confirms the phenotypes of novel perturbations. Our wild type and mutant simulations provide novel insights to restore apoptosis in defective cells. The model analysis expands our understanding of how cell fate decision is made. Moreover, our current model can be used to assess contradictory or controversial data from the literature. Ultimately, it constitutes a valuable reasoning tool to delineate novel experiments

Immunoproteasome LMP2 60HH Variant Alters MBP Epitope Generation and Reduces the Risk to Develop Multiple Sclerosis in Italian Female Population

Background: Albeit several studies pointed out the pivotal role that CD4+T cells have in Multiple Sclerosis, the CD8+ T cells involvement in the pathology is still in its early phases of investigation. Proteasome degradation is the key step in the production of MHC class I-restricted epitopes and therefore its activity could be an important element in the activation and regulation of autoreactive CD8+ T cells in Multiple Sclerosis. Methodology/Principal Findings: Immunoproteasomes and PA28-ab regulator are present in MS affected brain area and accumulated in plaques. They are expressed in cell types supposed to be involved in MS development such as neurons, endothelial cells, oligodendrocytes, macrophages/macroglia and lymphocytes. Furthermore, in a genetic study on 1262 Italian MS cases and 845 controls we observed that HLA-A*02+ female subjects carrying the immunoproteasome LMP2 codon 60HH variant have a reduced risk to develop MS. Accordingly, immunoproteasomes carrying the LMP2 60H allele produce in vitro a lower amount of the HLA-A*0201 restricted immunodominant epitope MBP111\u2013119. Conclusion/Significance: The immunoproteasome LMP2 60HH variant reduces the risk to develop MS amongst Italian HLAA* 02+ females. We propose that such an effect is mediated by the altered proteasome-dependent production of a specific MBP epitope presented on the MHC class I. Our observations thereby support the hypothesis of an involvement of immunoproteasome in the MS pathogenesis