Changes in IP3 receptor expression and function in aortic smooth muscle of atherosclerotic mice

Peroxynitrite is an endothelium - independent vasodilator which induces relaxation via membrane hyperpolarisation. A ctivation of IP3 receptors triggers opening of potassium channels and hyperpolarisation. Previously we found that relaxation to peroxynitrite was maintained during development of atherosclerosis due to changes in expression of calcium regulatory proteins. In this study we investigated 1) the mechanism of peroxynitrite - induced relaxation in mouse aorta 2) the effect of atherosclerosis on relaxation to peroxynitrite and other vasodilators 3) the effect of atherosclerosis on expression and function of the IP3 receptor. Aortic function was studied using wire myography and atherosclerosis was induced by fat - feeding ApoE - / - mice . Expression of IP3 receptors was studied using Western blotting and immunohistochemistry . Relaxation to peroxynitrite was attenuated by the IP3 antagonists 2 - APB and xestospongin C and also the Kv channel blocker 4 - AP. Atherosclerosis attenuated vasodilation to cromakalim and the AMPK activator A769662 but not peroxynitrite. Relaxation was attenuated to a greater extent by 2 - APB in atherosclerotic aortae despite reduced expression of IP3 receptors. 4 - AP was less effective in 4 month fat fed ApoE - / - mice. Peroxynitrite relaxation involves IP3 - induced calcium release and K V channel activation. This mechanism becomes less important as atherosclerosis develops and relaxation to peroxynitrite may be maintained by increased calcium extrusion

MODERN METHODOLOGICAL APPROACHES TO THE INVESTIGATION OF ENSURING AND PROTECTION OF HUMAN RIGHT TO ACCESS TO PUBLIC INFORMATION

The purpose of the paper is to study the preconditions of the necessity to use new scientific tools during the study of issues related to the ensuring and protection of human right to access to public information; to characterize the main scientific approaches to modern researches in the sphere of safeguarding human right to access to public information; to identify promising directions for further research on this topic. Methodology. The paper presents the examples of a negative use of scientific methodology in conducting research in the area of administrative law and procedure and also draws attention to the need to reconsider an obsolete scientific methodology that does not correspond to the current realities and negatively affects the development of legal science. It indicates and characterizes the main preconditions of the necessity to use new scientific tools while investigating the issues related to ensuring and protection of human right to access to public information. In the context of the research of peculiarities of administrative and legal coverage and protection of human right to access to public information, it is proposed to use the systems approach, which is extremely effective in analysing complicated legal phenomena and processes and permits to study the peculiarities of substantive, procedure and procedural legal regulation as a cohesive whole. Practical implications. The system approach is an extremely promising technique for studying legal reality. Its use allows synthesizing all the knowledge about legal phenomena (legal rules, legal relations, legal consciousness, etc.), which is accumulated during the past stages of formation and development of domestic legal science, for a deeper insight into the development and functioning of law in general and administrative law in particular. Value/originality. Problems that exist in legal science in general and in the science of administrative law, in particular, can be solved only with the help of new scientific tools, specifically, by applying the system approach. The use of the systems approach in case of studying the issues of ensuring and protection of the human right to access to public information would allow combining all studies that were performed on this issue in substantive, procedure or procedural parts of the administrative law

Accumulation of natural and artificial radionuclides in water and hydrobionts of fishing ponds of Dnipropetrovsk region

The paper presents the results of radioecological studies conducted in fish farms of the Dnipropetrovsk region. The distribution of radionuclides of natural and artificial origin is an urgent problem for various types of ecosystems, given the different routes of entry of these substances. The level of accumulation of radionuclides in the waters of the Zelenodolsk, Krynychansk and Kryvorizk fish farms was determined in a course of the presented study prior to the 2022 russian invasion of Ukraine. Content indicator of radium-226 in water ranged from 3.8 to 7.4 Bq/kg, thorium-232 - from 2.95 to 44.4 Bq/kg, potassium-40 - from 74.2 to 82.4 Bq/kg. Obtained in the present study results evidence that bottom sediments significantly accumulate the radionuclides. The registered content of radium-226 in them was from 30.1 to 14.8 Bq/kg, thorium-232 - from 9.6 to 41.5 Bq/kg, potassium-40 from 134.8 to 182.2 Bq/kg. The coefficients of accumulation of natural radionuclides in water and bottom sediments was registered as ranging from 1.4 to 5 for radium-226, from 0.82 to 3.3 for thorium-232, from 1.72 to 2.26 for potassium-40. The content of artificial radionuclides in water and bottom sediments determined in the study corresponded with their biochemical properties. In water, their value ranged from 0.13 to 1.8 Bq/kg for cesium-137 and from 0.05 to 0.08 Bq/kg for strontium-90. Taking into account the fact that bottom sediments accumulate a significant proportion of pollutants from water, the determined indicators were significantly higher than those in water, namely, from 5.6 to 10.4 Bq/kg for cesium-137 and from 1.5 to 2, 4 Bq/kg for strontium-90. The coefficients of accumulation of radionuclides of artificial origin in water and bottom sediments ranged from 3.3 to 80 for the content of cesium-137 and from 18.7 to 48 for the content of strontium-90. The levels of accumulation of natural radionuclides in the body of silver carp and carp were studied. The level of potassium-40 in the body of silver carp is lower than that of radium-226 and thorium-232 and ranges from 20.2 to 34.4 Bq/kg in all three fish farms. The same amount of radium-226 and thorium-232 was noted in the silver carp of the Krynychan fish farm which was on the level of 47 Bq/kg. In carp, the levels of natural radionuclides were significantly higher in all three fish farms. The content of cesium-137 was registered as approximately the same in silver carp and carp, with the exception of the Kryvyi Rih fish farm, where its level was significantly lower in the body of carp. Strontium-90 content was found in similar amounts in both types of fish. The concentration of radionuclides did not exceed the permissible levels

Information Technologies in Improving Crime Prevention Mechanisms in the Border Regions of Southern Ukraine

The purpose of this study is theoretical and methodological substantiation, deepening of the conceptual provisions and development of the scientific and practical recommendations on the formation of mechanisms for crime prevention in the border regions of the southern of Ukraine. The relevance of this study is due to the need to solve the problem crime by introducing precautionary measures in the border regions of the southern of Ukraine (Bondarenko et al., 2021).The study proved the direct dependence of the level and intensity of crime on the proximity of areas to areas of significant social and criminological tension, to areas of instability and armed conflict, which contributes to the militarization of the population and increasing psychological preparedness for conflict and its antisocial methods (Iatsyshyn et al., 2020). It is established that in the border regions of the south of Ukraine the growth of criminal crime for the studied period significantly exceeds the data for Ukraine.The threats to irredentism and separatism, international terrorism, organized crime of a transnational global scale are real for the southern regions of Ukraine: economic smuggling, smuggling of weapons, ammunition and means of terror, drug trafficking, unregulated migration, trafficking in human beings and their organs.Priorities for Ukraine have been set task of crime prevention: introduction of the newest technologies of protection of a law and order, attraction of scientific and industrial potential of regions, establishment of coordination and interaction between subjects of maintenance of frontier safety at all levels (frontier, national, interstate and international) and cross-border interaction with the international organizations of fight against organized crime.Based on international experience, crime prevention measures in the border regions of southern Ukraine are proposed in the following areas: general social precautionary measures; special criminological precautions;international cooperation in the development of cross-border cooperation;introduction of automated security systems and crime prevention; informational and educational activities of formationlegal culture among the population and the publi

Microfluidic contact lenses

Contact lens is a ubiquitous technology used for vision correction and cosmetics. Sensing in contact lenses has emerged as a potential platform for minimally invasive point-of-care diagnostics. Here, a microlithography method is developed to fabricate microconcavities and microchannels in a hydrogel-based contact lens via a combination of laser patterning and embedded templating. Optical microlithography parameters influencing the formation of microconcavities including ablation power (4.3 W) and beam speed (50 mm s−1) are optimized to control the microconcavity depth (100 µm) and diameter (1.5 mm). The fiber templating method allows the production of microchannels having a diameter range of 100–150 µm. Leak-proof microchannel and microconcavity connections in contact lenses are validated through flow testing of artificial tear containing fluorescent microbeads (Ø = 1–2 µm). The microconcavities of contact lenses are functionalized with multiplexed fluorophores (2 µL) to demonstrate optical excitation and emission capability within the visible spectrum. The fabricated microfluidic contact lenses may have applications in ophthalmic monitoring of metabolic disorders at point-of-care settings and controlled drug release for therapeutics

науково-практичний коментар

Закони України «Про державний захист працівників суду і правоохоронних органів», «Про державну охорону органів державної влади україни та посадових осіб»: науково-практичний коментар : станом на 27 травня 2020 року. – Київ : ВД «Професіонал», 2020. – 640 с. – ISBN 978-966-370-396-1.Законодавство України встановлює систему особливих заходів державного захисту працівників суду та правоохоронних органів від перешкоджання виконанню покладених на них законом обов'язків і здійсненню наданих прав, а так само від посягань на життя, здоров'я, житло та майно зазначених осіб, їх близьких родичів у зв'язку зі службовою діяльністю цих працівників. Основним правовим документом, що регламентує правовідносини у цій сфері є коментований Закон України «Про державний захист працівників суду і правоохоронних органів». Державною охороною органів державної влади України та посадових осіб (далі - державна охорона) вважається система організаційно-правових, режимних, оперативно-розшукових, інженерно-технічних та інших заходів, які здійснюються спеціально уповноваженими державними органами з метою забезпечення нормального функціонування органів державної влади України, безпеки посадових осіб та об’єктів, визначених законом. Таким законом є коментований Закон України «Про державну охорону органів державної влади України та посадових осіб». Окрім коментарів ця збірка законодавства пропонує актуальну судову практику та посилання на інше профільне законодавство із зазначених правовідносин.The commented Law of Ukraine "On state protection of employees of the court and law enforcement bodies" and the Law of Ukraine "On state protection of state authorities of Ukraine and officials" were provided. And also the collection offers current judicial practice and links to other specialized legislation on the specified legal relationship.Предоставлен комментируемый Закон Украины «О государственной защите работников суда и правоохранительных органов» и Закон Украины «О государственной охране органов государственной власти Украины и должностных лиц». А также в сборнике предложена актуальная судебная практика и ссылки на другое профильное законодательство по указанным правоотношениям

Wearables in medicine

Wearables as medical technologies are becoming an integral part of personal analytics, measuring physical status, recording physiological parameters, or informing schedule for medication. These continuously evolving technology platforms do not only promise to help people pursue a healthier life style, but also provide continuous medical data for actively tracking metabolic status, diagnosis, and treatment. Advances in the miniaturization of flexible electronics, electrochemical biosensors, microfluidics, and artificial intelligence algorithms have led to wearable devices that can generate real-time medical data within the Internet of things. These flexible devices can be configured to make conformal contact with epidermal, ocular, intracochlear, and dental interfaces to collect biochemical or electrophysiological signals. This article discusses consumer trends in wearable electronics, commercial and emerging devices, and fabrication methods. It also reviews real-time monitoring of vital signs using biosensors, stimuli-responsive materials for drug delivery, and closed-loop theranostic systems. It covers future challenges in augmented, virtual, and mixed reality, communication modes, energy management, displays, conformity, and data safety. The development of patient-oriented wearable technologies and their incorporation in randomized clinical trials will facilitate the design of safe and effective approaches

Financial information systems of the company Wall Street Systems

Bakalářská práce je zaměřena na finanční informační systémy - predevším na objasnění základních pojmů jako jsou informace, informační systémy a finanční informační systémy. Dále se tato práce popisuje finanční informační systémy podle světových výrobců jako SAP, Oracle, Microsoft. Hlavní část zahrnuje popis finančního informačního systému Wallstreet Suite, interakce komponentů této systémy a informační toky dat mezi komponenty. Na závěr je dáno porovnání vybranych finančních informačních systémů podle jejich možností.Bachelor's work is focused on financial information systems - especially in the clarification of basic terms such as information, information systems and financial information systems. Furthermore, this work describes the financial information systems in accordance with world's leading companies such as SAP, Oracle, Microsoft. The main part includes a description of the financial information system Wallstreet Suite, the interaction of components of the systems and information flows between components. The comparison of selected financial information systems according to their possibilities is given in the conclusion of the work.Ústav systémového inženýrství a informatikyDokončená práce s úspěšnou obhajobo

CONDUCTING FINANCIAL AND ECONOMIC EXAMINATIONS RELATED TO BANK FAILURE

According to experience of the banking system existence of Ukraine it is obvious that every year an increasing number of banks are negatively affected by the financial crisis, which is the result of a wave of liquidation and bankruptcy of banking institutions.&#x0D; Recently, a large number of requests have been sent to forensic institutions for conducting forensic economic examinations on the issues of a comprehensive analysis of the financial and economic activities of banks as well as on establishing features of concealing bankruptcy, fictitious bankruptcy or leading to bankruptcy. This is primarily due to the need to establish the possibility or impossibility of paying off monetary obligations including on deposits in the bank; analysis of the impact of certain management policies on the financial condition of banks; search for directions for optimizing costs and income of banks etc.&#x0D; While performing a forensic examination on concealing bankruptcy, fictitious bankruptcy or bringing bankruptcy within the scope of his competence, a forensic&#x0D;  expert shall give an opinion on the presence or absence at the enterprise of economic signs of fictitious bankruptcy, leading to bankruptcy or concealment of bankruptcy on the basis of the criteria defined in Guidelines No. 14. The calculation of these indicators for a bank is impossible due to the fact that there are differences in the determination of insolvency of the bank and the business entity due to the economic and legal nature of banks and the procedural steps for determining such insolvency.&#x0D; Currently, there is no legislatively approved methodology for determining the economic signs of bankruptcy, fictitious bankruptcy, hidden bankruptcy specifically for banks, which makes it impossible to calculate such financial and economic indicators of the bank as bankruptcy, fictitious bankruptcy, hidden bankruptcy.</jats:p