Increasing Entrepreneurship in Agriculture in an Economically Depressed Region

The article describes the purpose, conception, development, implementation, and evaluation of a series of workshops to support small/medium farms in a five-county area of northeastern North Carolina. Each of the workshops had a variety of topics to interest small and medium-sized farmers or those entering agriculture or agribusiness fields. The workshops, under a grant by the Golden Leaf Foundation, were to spur entrepreneurship within a rural, economically depressed region and implemented by an alliance of the Pasquotank County Center of the North Carolina Cooperative Extension Service and the School of Business & Economics at Elizabeth City State University

From Information Lost to Knowledge Gained: The Benefits of Analyzing all the Research Evidence

Data analyses should reveal truths about data. To the extent possible analyses should tell a complete picture. Data analyses should not inadvertently ignore phenomena that might be discovered in sample data sets. However, common univariate or multivariate data analysis methods tend to be based on only the means, standard deviations, and Pearson correlations. The result is that many important truths are discovered, but not the whole truth. This article illustrates in a sample data set that (a) data analyses of other properties of variables and groups are feasible and practical, and (b) such analyses may reveal important information not otherwise detectable. These extensions of common statistical methods are applicable to data analysis and interpretation issues in the social and behavioral sciences

One is Not Enough: The Need for Multiple Respondents in Survey Research of Organizations

The need for multiple respondents per organization in organizational survey research is supported. Leadership teams’ ratings of their implementations of market orientation are examined, along with learning orientation, entrepreneurial management, and organizational flexibility. Sixty diverse organizations, including not-for-profit organizations in education and healthcare as well as manufacturing and service businesses, were included. The major finding was the large rating variance within the leadership teams of each organization. The results are enlightening and have definite implications for improved design of survey research on organizations

Political consumerism as a neoliberal response to youth political disengagement

Recent trends indicate diminishing public engagement with formal electoral politics in many advanced liberal democracies, especially among the younger generations. However, evidence also suggests that there has been a simultaneous interest by many young citizens in political consumerism. In large part, this interest is shaped as a response to the individualisation and strict ‘economism’ driven by the underlying forces of neoliberalism. Disenfranchised and disillusioned by the seeming incapacity of the purely political sphere to respond to their individualised claims, and having internalised the neoliberal critique of democracy, these young empowered citizen-consumers often search for the ‘political’ within the bounds of the marketplace and are increasingly attracted to consumerist methods of political participation, such as boycotting and buycotting. Given the susceptibility of political consumerism to a neoliberal modus operandi, the lack of available literature problematising its emergence as a response to neoliberal principles is somewhat surprising. The present article will address this gap by connecting the declining levels of electoral participation among younger generations in post-crisis Europe to the rise of political consumerism within the neoliberal ideological hegemony of the ‘marketopoly’. We distinguish between two antithetical, but complimentary effects. Firstly, the internalised neoliberal critique of democracy emphasises the ‘push’ out of the public into the commercial sphere. Secondly, the emerging individualisation of modern ‘liquid’ politics advanced by the postmaterialist sensitivities of young people’s previously affluent socialisation call attention to the existence of a parallel ‘pull’ effect into the ‘marketopoly’, as a habitus of youth political participation. In both cases, the reorganisation of political participation as consumption, and the re-styling of young citizens as ‘empowered’ consumers, delineates political consumerism as an efficacious response to their political disengagement in an increasingly marketised world

Hormone replacement therapy and false positive recall in the Million Women Study: patterns of use, hormonal constituents and consistency of effect

INTRODUCTION: Current and recent users of hormone replacement therapy (HRT) have an increased risk of being recalled to assessment at mammography without breast cancer being diagnosed ('false positive recall'), but there is limited information on the effects of different patterns of HRT use on this. The aim of this study is to investigate in detail the relationship between patterns of use of HRT and false positive recall. METHODS: A total of 87,967 postmenopausal women aged 50 to 64 years attending routine breast cancer screening at 10 UK National Health Service Breast Screening Units from 1996 to 1998 joined the Million Women Study by completing a questionnaire before screening and were followed for their screening outcome. RESULTS: Overall, 399 (0.5%) participants were diagnosed with breast cancer and 2,629 (3.0%) had false positive recall. Compared to never users of HRT, the adjusted relative risk (95% CI) of false positive recall was: 1.62 (1.43–1.83), 1.80 (1.62–2.01) and 0.76 (0.52–1.10) in current users of oestrogen-only HRT, oestrogen-progestagen HRT and tibolone, respectively (p (heterogeneity) < 0.0001); 1.65 (1.43–1.91), 1.49 (1.22–1.81) and 2.11 (1.45–3.07) for current HRT used orally, transdermally or via an implant, respectively (p (heterogeneity) = 0.2); and 1.84 (1.67–2.04) and 1.75 (1.49–2.06) for sequential and continuous oestrogen-progestagen HRT, respectively (p (heterogeneity) = 0.6). The relative risk of false positive recall among current users appeared to increase with increasing time since menopause, but did not vary significantly according to any other factors examined, including duration of use, hormonal constituents, dose, whether single- or two-view screening was used, or the woman's personal characteristics. CONCLUSION: Current use of oestrogen-only and oestrogen-progestagen HRT, but not tibolone, increases the risk of false positive recall at screening

Alpha-2-Macroglobulin Is Acutely Sensitive to Freezing and Lyophilization: Implications for Structural and Functional Studies.

Alpha-2-macroglobulin is an abundant secreted protein that is of particular interest because of its diverse ligand binding profile and multifunctional nature, which includes roles as a protease inhibitor and as a molecular chaperone. The activities of alpha-2-macroglobulin are typically dependent on whether its conformation is native or transformed (i.e. adopts a more compact conformation after interactions with proteases or small nucleophiles), and are also influenced by dissociation of the native alpha-2-macroglobulin tetramer into stable dimers. Alpha-2-macroglobulin is predominately present as the native tetramer in vivo; once purified from human blood plasma, however, alpha-2-macroglobulin can undergo a number of conformational changes during storage, including transformation, aggregation or dissociation. We demonstrate that, particularly in the presence of sodium chloride or amine containing compounds, freezing and/or lyophilization of alpha-2-macroglobulin induces conformational changes with functional consequences. These conformational changes in alpha-2-macroglobulin are not always detected by standard native polyacrylamide gel electrophoresis, but can be measured using bisANS fluorescence assays. Increased surface hydrophobicity of alpha-2-macroglobulin, as assessed by bisANS fluorescence measurements, is accompanied by (i) reduced trypsin binding activity, (ii) increased chaperone activity, and (iii) increased binding to the surfaces of SH-SY5Y neurons, in part, via lipoprotein receptors. We show that sucrose (but not glycine) effectively protects native alpha-2-macroglobulin from denaturation during freezing and/or lyophilization, thereby providing a reproducible method for the handling and long-term storage of this protein.Early Career Fellowship from the National Health and Medical Research Council GNT1012521(A.R.W.); Wellcome Trust Programme Grant (J.R.K., C.M.D.) 094425/Z/10/Z; Samsung GRO Grant (M.R.W.)This is the final version of the article. It first appeared from PLoS via http://dx.doi.org/10.1371/journal.pone.013003

Host Genetic Factors and Vaccine-Induced Immunity to HBV Infection: Haplotype Analysis

Hepatitis B virus (HBV) infection remains a significant health burden world-wide, although vaccines help decrease this problem. We previously identified associations of single nucleotide polymorphisms in several candidate genes with vaccine-induced peak antibody level (anti-HBs), which is predictive of long-term vaccine efficacy and protection against infection and persistent carriage; here we report on a haplotype-based analysis. A total of 688 SNPs from 117 genes were examined for a two, three and four sliding window haplotype analysis in a Gambian cohort. Analysis was performed on 197 unrelated individuals, 454 individuals from 174 families, and the combined sample (N = 651). Global and individual haplotype association tests were carried out (adjusted for covariates), employing peak anti-HBs level as outcome. Five genes (CD44, CD58, CDC42, IL19 and IL1R1) had at least one significant haplotype in the unrelated or family analysis as well as the combined analysis. Previous single locus results were confirmed for CD44 (combined global p = 9.1×10−5 for rs353644-rs353630-rs7937602) and CD58 (combined global p = 0.008 for rs1414275-rs11588376-rs1016140). Haplotypes in CDC42, IL19 and IL1R1 also associated with peak anti-HBs level. We have identified strong haplotype effects on HBV vaccine-induced antibody level in five genes, three of which, CDC42, IL19 and IL1R1, did not show evidence of association in a single SNP analyses and corroborated the majority of these effects in two datasets. The haplotype analysis identified associations with HBV vaccine-induced immunity in several new genes

Ploidy variation in fungi: Polyploidy, aneuploidy, and genome evolution

The ability of an organism to replicate and segregate its genome with high fidelity is vital to its survival and for the production of future generations. Errors in either of these steps (replication or segregation) can lead to a change in ploidy or chromosome number. While these drastic genome changes can be detrimental to the organism, resulting in decreased fitness, they can also provide increased fitness during periods of stress. A change in ploidy or chromosome number can fundamentally change how a cell senses and responds to its environment. Here, we discuss current ideas in fungal biology that illuminate how eukaryotic genome size variation can impact the organism at a cellular and evolutionary level. One of the most fascinating observations from the past 2 decades of research is that some fungi have evolved the ability to tolerate large genome size changes and generate vast genomic heterogeneity without undergoing canonical meiosis

Patient blood management: A solution for South Africa

For more than 70 years the default therapy for anaemia and blood loss was mostly transfusion. Accumulating evidence demonstrates a significant dose-dependent relationship between transfusion and adverse outcomes. This and other transfusion-related challenges led the way to a new paradigm. Patient blood management (PBM) is the application of evidence-based practices to optimise patient outcomes by managing and preserving the patient’s own blood. ‘Real-world’ studies have shown that PBM improves patient outcomes and saves money. The prevalence of anaemia in adult South Africans is 31% in females and 17% in males. Improving the management of anaemia will firstly improve public health, secondly relieve the pressure on the blood supply, and thirdly improve the productivity of the nation’s workforce. While high-income countries are increasingly implementing PBM, many middle- and low-income countries are still trying to upscale their transfusion services. The implementation of PBM will improve South Africa’s health status while saving costs