Ethical conflicts among physicians and nurses during the COVID‐19 pandemic: A qualitative study

Rationale, Aims and Objectives The healthcare system and professionals working in the sector have experienced a high caseload during the coronavirus disease 2019 (COVID-19) pandemic. This has increased the potential for morally harmful events that violate professionals' moral codes and values. The aim of this study was to understand and explore experiences of new moral challenges emerging among physicians and nurses caring for individuals during the COVID-19 pandemic. Method The consolidated criteria for reporting qualitative research (COREQ) checklist was used in this qualitative study based on Gadamer's phenomenology. Participants were selected using a convenience sampling method. Thirteen medicine and nursing graduates were interviewed in depth. The participants all worked on the frontline at the start of the COVID-19 pandemic. Data were gathered in two basic healthcare districts in Spain, encompassing both primary care and hospital care. Results Four main themes emerged from the data analysis: (1) Betrayal of moral and ethical values as a key source of suffering; (2) Ethical and moral sense of failure accompanying loss of meaning; (3) Lack of confidence in performance; (4) Self-demand and self-punishment as personal condemnation among healthcare workers. Conclusions Health institutions must implement interventions for health professionals to help mitigate the consequences of experiencing complex ethical scenarios during the pandemic. In addition, they should promote training in moral and ethical deliberation and prepare them to make decisions of great ethical significance

Use of image analysis to evaluate the effect of high hydrostatic pressure and pasteurization as preservation treatments on the microstructure of red sweet pepper

[EN] The aim of this work was to evaluate the effect of HHP treatment and PAST on the microstructure of red Lamuyotype sweet peppers using image analysis and to determine the parameters that allow characterizing the changes observed on the structure using different magnifications (100×, 200×, and 350×). The results show that all the preservation treatments evaluated caused structural modifications on the microstructure of red sweet pepper, but HHP at 500 MPa and PAST had less impact. Fractal dimension texture, contrast, inverse difference moment, and entropy are texture features that are appropriate for characterizing the effect of HHP and PAST on red pepper texture. In this context, it is important to consider the magnification at which red pepper texture is evaluated because cell damage caused by treatments is best observed at low magnification. Consequently, image analysis could be used in future studies to relate microstructure to the functionality of products subject to HHP. Industrial relevance: Red sweet peppers (Capsicum annuum) are an excellent source of essential nutrients and bioactive compounds. High hydrostatic pressure (HHP) applied during food processing can improve the retention of food quality attributes and nutritional and organoleptic properties better than pasteurization (PAST). Image analysis is a non-invasive technique that allows to provide objective evaluations from digitalized images. There are no studies that quantify the effect of HHP and PAST using image texture parameters and that evaluate the effect of the magnification used on these texture features. These features are critical factors that determine food acceptance or rejection by the consumers. Thus, texture measurement has gained much attention from food science and industry. Therefore, it would be interesting to study the effect of these treatments on the microstructure of red sweet pepper tissue using image analysis. Thereby, it would be possible to relate the image information to structural modifications and to the extractability of bioactive compounds or acceptance of preservation processes by consumers.The authors wish to acknowledge the Spanish Ministry of Science and Innovation for the financial support (project AGL2011-30064-C02) and to the Universitat Politecnica de Valencia (UPV) for the FPI grant given to Maria Hernandez Carrion. The authors also thank University of La Sabana for let Maria Hernandez Carrion to do a stay of research and for access to their materials and methods. Moreover, the authors wish to thank Elsevier for assistance with the English manuscriptHernández-Carrión, M.; Hernando Hernando, MI.; Sotelo-Díaz, I.; Quintanilla-Carvajal, M.; Quiles Chuliá, MD. (2015). Use of image analysis to evaluate the effect of high hydrostatic pressure and pasteurization as preservation treatments on the microstructure of red sweet pepper. Innovative Food Science & Emerging Technologies. 27:69-78. https://doi.org/10.1016/j.ifset.2014.10.011S69782

Strawberry tree honey in combination with 5-fluorouracil enhances chemosensitivity in human colon adenocarcinoma cells

The authors would like to thank Prof. Gavino Sanna, Department of Chemistry and Pharmacy (University of Sassari, Italy), for providing STH samples. Tamara Y. Forbes-Hernandez is supported by a "Juan de la Cierva-Formacion" post-doctoral contract.Colorectal cancer remains a challenging health burden worldwide. This study aimed to assess the potentiality of Strawberry tree honey (STH), a polyphenol-enriched food, to increase the effectiveness of 5-Fluorouracil (5-FU) in adenocarcinoma (HCT-116) and metastatic (LoVo) colon cancer cell lines. The combined treatment reduced cell viability and caused oxidative stress, by increasing oxidative biomarkers and decreasing antioxidant defence, in a more potent way compared to 5-FU alone. The expression of endoplasmic reticulum (ATF-6, XBP-1) and MAPK (p-p38 MAPK, p-ERK1/2) markers were also elevated after the combined treatment, enhancing the cell cycle arrest through the modulation of regulatory genes (i.e., cyclins and CDKs). Apoptotic gene (i.e., caspases) expressions were also increased after the combined treatment, while those of proliferation (i.e., EGFR), cell migration, invasion (i.e., matrix metallopeptidase) and epithelial–mesenchymal transition (N-cadherin, β-catenin) were suppressed. Finally, the combined treatment led cell metabolism towards a quiescent stage, by reducing mitochondrial respiration and glycolysis. In conclusion, this work represents an initial step to highlight the possibility to use STH in combination with 5-FU in the treatment of colon cancer, even if further in vitro an in vivo studies are strongly needed to confirm the possible chemo-sensitizing effects of STH."Juan de la Cierva-Formacion" post-doctoral contrac

Oxidative stress status in an institutionalised elderly group after the intake of a phenolic-rich dessert

The present study was supported by a grant from Hero España S.A. M. C. R.-T. and J. L. Q. are recipients of a ‘Ramón y Cajal’ contract from the Ministry of Science and Technology and the University of Granada, Spain. We also thank the Fundacio´n Se´neca of the C.A.R.M. (Murcia, Spain) for the fellowship of J. G.-A.The elderly population undergoes a series of physiological and sociological changes common to old age with a high probability of suffering degenerative illness and malnutrition. A dessert rich in phenolic compounds has been designed by using concentrated juices of grape, cherry, blackberry, blackcurrant and raspberry with the aim of it being used as a complementary food in adulthood. In the present study, we investigated the effect of the intake of this dessert (a jar of 200 g daily for a period of 2 weeks), with an antioxidant activity equivalent to ten servings of fruits and vegetables, on several markers of oxidative and antioxidant status in DNA and plasma in a group of elderly individuals. Non-smoking institutionalised elderly subjects were recruited from a pool of volunteers in an old-age home in Murcia (Spain). Twenty-two subjects (six men and sixteen women) participated in the study. The study was designed as a randomised intervention trial with a period of 2 weeks. At days 1 and 15, blood samples were collected to analyse total antioxidant capacity, biochemical parameters, antioxidant vitamins, LDL peroxidation, and DNA damage in peripheral blood lymphocytes. The conclusion of the present study is that a 2-week intervention with our dessert enriched with natural polyphenol compounds in elderly individuals does not give enough time to find changes in the antioxidant and oxidative status. Also, the view that the marked antioxidant ability of polyphenols in vitro does not translate to analogous effects in vivo was confirmed. Moreover, a highly oxidative stress status during ageing was confirmed, together with the need to perform follow-up nutritional studies to improve this situation

On natural metalinguistic abilities in aphasia: a preliminary study

Natural metalinguistic abilities, which are put into play without explicit instructions, constitute the cognitive basis for a 'reflexive' use of language, a particular manifestation of the executive function when applied to language and verbal behaviour. This reflexive use entails a specific attentional activity by speakers and hearers with regard to linguistic outputs, and an intentional experience-based control over the language use. Putting into play natural metalinguistic abilities can be considered a significant factor for explaining different kinds of adaptive processes. Our results permit us to conclude that an impairment of metalinguistic abilities is involved in aphasia to different degrees. Moreover, the examination of preserved metalinguistic abilities provides an alternative way for assessing the degree of severity of impaired communicative behaviour by people with aphasia. Our procedure, presumably, will also be useful for suggesting new factors when designing therapeutic programmes

Ethical conflicts among physicians and nurses during the COVID‐19 pandemic: A qualitative study

Rationale, Aims and Objectives The healthcare system and professionals working in the sector have experienced a high caseload during the coronavirus disease 2019 (COVID-19) pandemic. This has increased the potential for morally harmful events that violate professionals' moral codes and values. The aim of this study was to understand and explore experiences of new moral challenges emerging among physicians and nurses caring for individuals during the COVID-19 pandemic. Method The consolidated criteria for reporting qualitative research (COREQ) checklist was used in this qualitative study based on Gadamer's phenomenology. Participants were selected using a convenience sampling method. Thirteen medicine and nursing graduates were interviewed in depth. The participants all worked on the frontline at the start of the COVID-19 pandemic. Data were gathered in two basic healthcare districts in Spain, encompassing both primary care and hospital care. Results Four main themes emerged from the data analysis: (1) Betrayal of moral and ethical values as a key source of suffering; (2) Ethical and moral sense of failure accompanying loss of meaning; (3) Lack of confidence in performance; (4) Self-demand and self-punishment as personal condemnation among healthcare workers. Conclusions Health institutions must implement interventions for health professionals to help mitigate the consequences of experiencing complex ethical scenarios during the pandemic. In addition, they should promote training in moral and ethical deliberation and prepare them to make decisions of great ethical significance

Amyloid β-but not Tau-induced neurotoxicity is suppressed by Manuka honey via HSP-16.2 and SKN-1/Nrf2 pathways in an in vivo model of Alzheimer’s disease

Alzheimer’s is a chronic degenerative disease of the central nervous system considered the leading cause of dementia in the world. It is characterized by two etiopathological events related to oxidative stress: the aggregation of β-amyloid peptide and the formation of neurofibrillary tangles of hyperphosphorylated Tau protein in the brain. The incidence of this disease increases with age and has been associated with inadequate lifestyles. Some natural compounds have been shown to improve the hallmarks of the disease. However, despite its potential, there is no scientific evidence about Manuka honey (MH) in this regard. In the present work we evaluated the effect of MH on the toxicity induced by Aβ aggregation and Tau in a Caenorhabditis elegans model. Our results demonstrated that MH was able to improve indicators of oxidative stress and delayed Aβ-induced paralysis in the AD model CL4176 through HSP-16.2 and SKN-1/ NRF2 pathways. Nevertheless, its sugar content impaired the indicators of locomotion (an indicator of tau neurotoxicity) in both the transgenic strain BR5706 and in the wild-type N2 worms.MCIN/AEI FPU2017/04358FSE "El FSE invierte en tu futuro" FPU2018/05301JdC-I post-doctoral contract - NextGenerationEU IJC2020-043910-IFEDER/Junta de Andalucia-Consejeria de Economia y Conocimiento B-AGR-193-UGR1

Comprehensive profiling of mammalian Tribbles interactomes implicates TRIB3 in gene repression

The three human Tribbles (TRIB) pseudokinases have been implicated in a plethora of signaling and metabolic processes linked to cancer initiation and progression and can potentially be used as biomarkers of disease and prognosis. While their modes of action reported so far center around protein–protein interactions, the comprehensive profiling of TRIB interactomes has not been reported yet. Here, we have developed a robust mass spectrometry (MS)-based proteomics approach to characterize Tribbles’ interactomes and report a comprehensive assessment and comparison of the TRIB1, -2 and -3 interactomes, as well as domain-specific interactions for TRIB3. Interestingly, TRIB3, which is predominantly localized in the nucleus, interacts with multiple transcriptional regulators, including proteins involved in gene repression. Indeed, we found that TRIB3 repressed gene transcription when tethered to DNA in breast cancer cells. Taken together, our comprehensive proteomic assessment reveals previously unknown interacting partners and functions of Tribbles proteins that expand our understanding of this family of proteins. In addition, our findings show that MS-based proteomics provides a powerful tool to unravel novel pseudokinase biology

La enseñanza de la lengua de signos en el Espacio Europeo de Educación Superior: Adaptaciones al Marco Común Europeo de Referencia para las Lenguas

La red de investigación que, desde la Universidad de Alicante, analiza la enseñanza de la lengua de signos española (LSE) en el marco del Espacio Europeo de Educación Superior (EEES), se ha articulado en torno a tres ejes: la propia enseñanza de la lengua y la cultura de la Comunidad Sorda española en el ámbito universitario y, más concretamente, en la Universidad de Alicante; el aprendizaje por parte del alumnado que ha cursado esta materia, y el papel de las intérpretes de lengua de signos que desarrollan su labor en este contexto. En los tres casos, presentamos la investigación que hemos llevado a cabo desde la red gracias a la colaboración de profesorado, alumnado e intérpretes, y que supone una completa panorámica de la situación actual de la LSE en el EEES. Una de las principales conclusiones es la necesidad de armonizar su enseñanza bajo las directrices europeas de enseñanza de idiomas que dicta el Marco Común Europeo de Referencia para las Lenguas (MCER, 2001), lo cual presupone una estandarización lingüística de la LSE que aún está en vías de desarrollo

Decapping Protein Edc4 Regulates Dna Repair And Phenocopies Brca1

BRCA1 is a tumor suppressor that regulates DNA repair by homologous recombination. Germline mutations in BRCA1 are associated with increased risk of breast and ovarian cancer and BRCA1 deficient tumors are exquisitely sensitive to poly (ADP-ribose) polymerase (PARP) inhibitors. Therefore, uncovering additional components of this DNA repair pathway is of extreme importance for further understanding cancer development and therapeutic vulnerabilities. Here, we identify EDC4, a known component of processing-bodies and regulator of mRNA decapping, as a member of the BRCA1-BRIP1-TOPBP1 complex. EDC4 plays a key role in homologous recombination by stimulating end resection at double-strand breaks. EDC4 deficiency leads to genome instability and hypersensitivity to DNA interstrand cross-linking drugs and PARP inhibitors. Lack-of-function mutations in EDC4 were detected in BRCA1/2-mutation-negative breast cancer cases, suggesting a role in breast cancer susceptibility. Collectively, this study recognizes EDC4 with a dual role in decapping and DNA repair whose inactivation phenocopies BRCA1 deficiency