Interdisziplinäre Handlungsfähigkeit

Die Förderung interdisziplinärer Handlungsfähigkeit bei  Studierenden bereitet auf Herausforderungen der postmodernen Gesellschaft sowie auf komplexe berufliche Aufgaben vor und ergänzt hochspezialisierte Studiengänge. In diesem Artikel werden vier an der Hochschule Coburg erprobte Module zum Erwerb interdisziplinärer Handlungsfähigkeit (IHA) mithilfe des IHA-Modells systematisierend beschrieben. Damit wird die Grundlage für einen Dialog über geeignete Lehr-Lern-Settings für interdisziplinäre Lehre geschaffen

Graphene Liquid Enclosure for Single-Molecule Analysis of Membrane Proteins in Whole Cells Using Electron Microscopy.

Membrane proteins govern many important functions in cells via dynamic oligomerization into active complexes. However, analytical methods to study their distribution and functional state in relation to the cellular structure are currently limited. Here, we introduce a technique for studying single-membrane proteins within their native context of the intact plasma membrane. SKBR3 breast cancer cells were grown on silicon microchips with thin silicon nitride windows. The cells were fixed, and the epidermal growth factor receptor ErbB2 was specifically labeled with quantum dot (QD) nanoparticles. For correlative fluorescence- and liquid-phase electron microscopy, we enclosed the liquid samples by chemical vapor deposited (CVD) graphene films. Depending on the local cell thickness, QD labels were imaged with a spatial resolution of 2 nm at a low electron dose. The distribution and stoichiometric assembly of ErbB2 receptors were determined at several different cellular locations, including tunneling nanotubes, where we found higher levels of homodimerization at the connecting sites. This experimental approach is applicable to a wide range of cell lines and membrane proteins and particularly suitable for studies involving both inter- and intracellular heterogeneity in protein distribution and expression

Giant radiolytic dissolution rates of aqueous ceria observed in-situ by liquid-cell TEM

Dynamics of cerium oxide nanoparticle aqueous corrosion are revealed in-situ. We use innovative liquid-cell transmission electron microscopy (TEM) combined with deliberate high-intensity electron-beam irradiation of nanoparticle suspensions. This enables life video-recording of materials reactions in liquid, with nm-resolution. We introduce image-quantification to measure detailed rates of dissolution as a function of time and particle size to be compared with literature data. Giant dissolution rates, exceeding any previous reports for chemical dissolution rates at room temperature by many orders of magnitude, are discovered. Reasons for accelerated dissolution are outlined, including the importance of radiolysis of water preceding ceria-attack. Electron-water interaction generates radicals, ions and hydrated electrons, which assist in hydration and reductive dissolution of oxide minerals. The presented methodology has the potential to become a novel accelerated testing procedure to compare multiple nanoscale materials for relative aqueous durability. The ceria-water system is of crucial importance for the fields of catalysis, abrasive polishing, environmental remediation, and as simulant for actinide-oxide behaviour in contact with liquid for nuclear engineering

Post-functionalized iridium Zr-MOF as a promising recycle catalyst for the hydrogenation of aromatics

[EN] The multifunctional heterogeneous catalyst iridium–Zr-based MOF is able to effectively catalyze the hydrogenation of aromatic compounds in high yields under mild conditions. The catalyst was found to be highly active and reusable, giving similar reactivity and selectivity after at least five catalytic uses.We thank the MINECO of Spain (project MAT2011-29020-C02-02), Consolider-Ingenio 2010-(CSD-0050-MULTICAT). for financial support. A.M.R.A. thanks MINECO for the FPI program.Rasero Almansa, AM.; Corma Canós, A.; Iglesias, M.; Sánchez Alonso, F. (2014). Post-functionalized iridium Zr-MOF as a promising recycle catalyst for the hydrogenation of aromatics. Green Chemistry. 16(7):3522-3527. https://doi.org/10.1039/c4gc00581cS3522352716

Mission Analysis of the Nanosatellite Sonate

The objective of this thesis is the development of a software application facilitating a quick and extensive mission analysis including all subsystems of the satellite. Furthermore mission analysis for the Sonate mission is done, utilizing the developed program. Usually mission analysis is done by applying separately different tools which are specific for each subsystem. The drawback of this method is that the system is not analyzed as a whole; rather all subsystems are investigated separately from each other. In case one or more system parameters should change, the effect of this variation onto other parts of the system will not directly be accessible and visible. A simulation tool considering all these subsystems overcomes this weaknesses and helps to develop a balanced system. Different system configurations and operation scenarios can be evaluated quickly and compared to each other in order to find an optimal solution. The simulation system developed during this thesis exhibits a client-server structure, where the subsystems are separated modules acting as clients. The program has a highly modular structure which renders it possible to have an arbitrary extension in the future. Aside from the server, connecting all modules, the following modules were implemented: orbital dynamics module containing orbit propagation and attitude determination power subsystem consisting of the subparts power production, power consumption and power storage archiving module saving all results generated for further evaluation simulation clock providing the simulation time configuration module with a GUI to facilitate the configuration of all simulation parameters simple thermal module providing temperatures for the satellite panels Mission analysis was done for the Sonate satellite utilizing the developed program in order to find a configuration for the solar panels of the satellite such that all the subsystems of the satellite can be supplied with sufficient power during the mission

Studying dynamic processes of nano-sized objects in liquid using scanning transmission electron microscopy

Samples fully embedded in liquid can be studied at a nanoscale spatial resolution with Scanning Transmission Electron Microscopy (STEM) using a microfluidic chamber assembled in the specimen holder for Transmission Electron Microscopy (TEM) and STEM. The microfluidic system consists of two silicon microchips supporting thin Silicon Nitride (SiN) membrane windows. This article describes the basic steps of sample loading and data acquisition. Most important of all is to ensure that the liquid compartment is correctly assembled, thus providing a thin liquid layer and a vacuum seal. This protocol also includes a number of tests necessary to perform during sample loading in order to ensure correct assembly. Once the sample is loaded in the electron microscope, the liquid thickness needs to be measured. Incorrect assembly may result in a too-thick liquid, while a too-thin liquid may indicate the absence of liquid, such as when a bubble is formed. Finally, the protocol explains how images are taken and how dynamic processes can be studied. A sample containing AuNPs is imaged both in pure water and in saline