EcoRI restriction endonuclease cleavage site map of bacteriophage P22 DNA

The seven EcoRI restriction endonuclease cleavage sites in bacteriophage P22 DNA have been mapped. The cleavage site map of circularly permuted P22 linear DNA is a circle. The positions of EcoRI sites in the early region of the P22 genome were determined by comparing products of EcoRI digestion of mature linear P22 chromosomes with the EcoRI cleavage fragments of DNA of three [lambda]immP22 hybrid phages and a P22 deletion mutant. An EcoRI site in the late region was mapped by partial digestion of the P22 deletion mutant DNA. Since the P22 genes included in the [lambda]immP22 hybrid phages, and the genetic region missing in the P22 deletion mutant are known, some P22 genes can be positioned on this initial physical map of the P22 genome relative to EcoRI cleavage sites.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/22676/1/0000229.pd

Magnetic Flux Density Sensor Analysis

Undergraduate Basi

The Asian decapod Hemigrapsus penicillatus (de Haan, 1835) (Grapsidae, Decapoda) introduced in European waters: status quo and future perspectives

The Asian decapod Hemigrapsus penicillatus (de Haan, 1835) was first recorded in European waters in 1994. The first specimens were collected in the estuary of Charente Maritime on the west coast of France close to La Rochelle. The current range in Europe covers Spanish shallow water habitats of the Bay of Biscay to areas north of La Rochelle (France). Densities of up to 20 specimens per square metre occur. This species has a high temperature and salinity tolerace and will expand its distribution in European waters. It is not clear whether this crab was introduced by shipping in ballast water or as a fouling organism. Based on a study of ship hull fouling in German dry docks this account provides evidence that hull fouling is a likely vector for the introduction of this crab. In August 1993, six juvenile specimens of H. penicillatus were removed from the hull of a car-carrier. After its journey from Japan into European waters this vessel docked in the port of Bremerhaven (Germany) for a routine inspection and coating with antifouling paint