Application of Luminex Gastrointestinal Pathogen Panel to human stool samples from Côte d'Ivoire

Gastrointestinal infections caused by viruses, bacteria, and parasites are endemic in most developing countries due to inadequate provision of safe water supplies, sanitation, and hygiene. To investigate the enteric pathogens infecting people living in Côte d'Ivoire, the Luminex Gastrointestinal Pathogen Panel (xTAG GPP) assay was used to analyze 34 human fecal samples. This study represents the first application of this technology to samples from a sub-Saharan African country

Technical note: Introduction of a superconducting gravimeter as novel hydrological sensor for the Alpine research catchment Zugspitze

GFZ (German Research Centre for Geosciences) set up the Zugspitze Geodynamic Observatory Germany with a worldwide unique installation of a superconducting gravimeter at the summit of Mount Zugspitze on top of the Partnach spring catchment. This high alpine catchment is well instrumented, acts as natural lysimeter and has significant importance for water supply to its forelands, with a large mean annual precipitation of 2080ĝ€¯mm and a long seasonal snow cover period of 9 months, while showing a high sensitivity to climate change. However, regarding the majority of alpine regions worldwide, there is only limited knowledge on temporal water storage variations due to sparsely distributed hydrological and meteorological sensors and the large variability and complexity of signals in alpine terrain. This underlines the importance of well-equipped areas such as Mount Zugspitze serving as natural test laboratories for improved monitoring, understanding and prediction of alpine hydrological processes. The observatory superconducting gravimeter, OSG 052, supplements the existing sensor network as a novel hydrological sensor system for the direct observation of the integral gravity effect of total water storage variations in the alpine research catchment at Zugspitze. Besides the experimental set-up and the available data sets, the gravimetric methods and gravity residuals are presented based on the first 27 months of observations from 29 December 2018 to 31 March 2021. The snowpack is identified as being a primary contributor to seasonal water storage variations and, thus, to the gravity residuals with a signal range of up to 750ĝ€¯nms-2 corresponding to 1957ĝ€¯mm snow water equivalent measured with a snow scale at an altitude of 2420ĝ€¯m at the end of May 2019. Hydro-gravimetric sensitivity analysis reveal a snow-gravimetric footprint of up to 4ĝ€¯km distance around the gravimeter, with a dominant gravity contribution from the snowpack in the Partnach spring catchment. This shows that the hydro-gravimetric approach delivers representative integral insights into the water balance of this high alpine site. © Copyright

Role of blood pressure in mediating carotid artery dilation in response to sympathetic stimulation in healthy, middle-aged individuals

Objectives: Carotid artery diameter responses to sympathetic stimulation, i.e., carotid artery reactivity (CAR), represent a novel test of vascular health and relates to cardiovascular disease (CVD)/risk. This study aims to understand the relationship between the increase in blood pressure and carotid artery diameter response during the CAR-test in healthy, middle-aged men. Methods: Sample consisted of 40 normotensive men (aged 31–59 years) with no history of CVD of currently taking medication. Noninvasive ultrasound was used to measure carotid artery diameter during the cold pressor test (CPT), with CAR% being calculated as the relative change from baseline (%). Mean arterial pressure (MAP) was measured with beat-to-beat blood pressure recording. Results: CAR% was 4.4 ± 5.4%, peaking at 92 ± 43 seconds. MAP increased from 88 ± 9 mmHg to 110 ± 15 mmHg, peaked at 112 ± 38 seconds, which was significantly later than the diameter peak (P = 0.04). The correlation between resting MAP and CAR% was weak (r = 0.209 P = 0.197). Tertiles based on resting MAP or MAP-increase revealed no significant differences between groups in subject characteristics including age, body mass index, or CAR% (all P > 0.05). Subgroup analysis of individuals with carotid constriction (n = 6) vs. dilation (n = 34), revealed no significant difference in resting MAP or increase in MAP (P = 0.209 and 0.272, respectively). Conclusion: Our data suggest that the characteristic increase in MAP during the CPT does not mediate carotid artery vasomotion

Role of the 5-Lipoxygenase–activating Protein (FLAP) in Murine Acute Inflammatory Responses

Leukotrienes are potent inflammatory mediators synthesized from arachidonic acid (AA) predominately by cells of myeloid origin. The synthesis of these lipids is believed to be dependent not only on the expression of the enzyme 5-lipoxygenase (5-LO), which catalyzes the first steps in the synthesis of leukotrienes, but also on expression of a nuclear membrane protein termed the 5-LO–activating protein (FLAP). To study the relationship of these two proteins in mediating the production of leukotrienes in vivo and to determine whether the membrane protein FLAP has additional functions in various inflammatory processes, we have generated a mouse line deficient in this protein. FLAP-deficient mice develop normally and are healthy. However, an array of assays comparing inflammatory reactions in FLAP-deficient mice and in normal controls revealed that FLAP plays a role in a subset of these reactions. Although examination of DTH and IgE-mediated passive anaphylaxis showed no difference between wild-type and FLAP-deficient animals, mice without FLAP possessed a blunted inflammatory response to topical AA and had increased resistance to platelet-activating factor–induced shock compared to controls. Also, edema associated with Zymosan A–induced peritonitis was markedly reduced in animals lacking FLAP. To determine whether these differences relate solely to a deficit in leukotriene production, or whether they reflect an additional role for FLAP in inflammation, we compared the FLAP-deficient mice to 5-LO–deficient animals. Evaluation of mice lacking FLAP and 5-LO indicated that production of leukotrienes during inflammatory responses is dependent upon the availability of FLAP and did not support additional functions for FLAP beyond its role in leukotriene production

Final report of EURAMET.M.G-K3 regional comparison of absolute gravimeters

The regional key comparison of absolute gravimeters, EURAMET.M.G-K3 and the simultaneously organized additional comparison, was held in Germany at the Geodetic Observatory Wettzell of the German Federal Agency for Cartography and Geodesy in the spring of 2018.Here we present the list of the participants who actually performed measurements during the comparison, the data submitted by the operators as well as the results of the determination of the gravity as a function of height at the comparison sites. The measurement strategy is briefly discussed and the results of the data harmonization is documented. Finally, the results of the constrained least squares adjustment are presented including the degrees of equivalence of each gravimeter and the key comparison reference values.Main textTo reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.The final report has been peer-reviewed and approved for publication by the CCM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).Export citation and abstract BibTeX RIS

Dietary fish oil supplements depress milk fat yield and alter milk fatty acid composition in lactating cows fed grass silage-based diets

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Geodesy and metrology with a transportable optical clock

partially_open24openGrotti, Jacopo; Koller, Silvio; Vogt, Stefan; Häfner, Sebastian; Sterr, Uwe; Lisdat, Christian; Denker, Heiner; Voigt, Christian; Timmen, Ludger; Rolland, Antoine; Baynes, Fred N.; Margolis, Helen S.; Zampaolo, Michel; Thoumany, Pierre; Pizzocaro, Marco; Rauf, Benjamin; Bregolin, Filippo; Tampellini, Anna; Barbieri, Piero; Zucco, Massimo; Costanzo, Giovanni A.; Clivati, Cecilia; Levi, Filippo; Calonico, DavideGrotti, Jacopo; Koller, Silvio; Vogt, Stefan; Häfner, Sebastian; Sterr, Uwe; Lisdat, Christian; Denker, Heiner; Voigt, Christian; Timmen, Ludger; Rolland, Antoine; Baynes, Fred N.; Margolis, Helen S.; Zampaolo, Michel; Thoumany, Pierre; Pizzocaro, Marco; Rauf, Benjamin; Bregolin, Filippo; Tampellini, Anna; Barbieri, Piero; Zucco, Massimo; Costanzo, Giovanni A.; Clivati, Cecilia; Levi, Filippo; Calonico, David

Altered mRNA expression of genes related to nerve cell activity in the fracture callus of older rats: A randomized, controlled, microarray study

BACKGROUND: The time required for radiographic union following femoral fracture increases with age in both humans and rats for unknown reasons. Since abnormalities in fracture innervation will slow skeletal healing, we explored whether abnormal mRNA expression of genes related to nerve cell activity in the older rats was associated with the slowing of skeletal repair. METHODS: Simple, transverse, mid-shaft, femoral fractures with intramedullary rod fixation were induced in anaesthetized female Sprague-Dawley rats at 6, 26, and 52 weeks of age. At 0, 0.4, 1, 2, 4, and 6 weeks after fracture, a bony segment, one-third the length of the femur, centered on the fracture site, including the external callus, cortical bone, and marrow elements, was harvested. cRNA was prepared and hybridized to 54 Affymetrix U34A microarrays (3/age/time point). RESULTS: The mRNA levels of 62 genes related to neural function were affected by fracture. Of the total, 38 genes were altered by fracture to a similar extent at the three ages. In contrast, eight neural genes showed prolonged down-regulation in the older rats compared to the more rapid return to pre-fracture levels in younger rats. Seven genes were up-regulated by fracture more in the younger rats than in the older rats, while nine genes were up-regulated more in the older rats than in the younger. CONCLUSIONS: mRNA of 24 nerve-related genes responded differently to fracture in older rats compared to young rats. This differential expression may reflect altered cell function at the fracture site that may be causally related to the slowing of fracture healing with age or may be an effect of the delayed healing

Observed secular gravity trend at Onsala station with the FG5 gravimeter from Hannover

Annual absolute gravity measurements with a FG5 instrument were performed in Onsala Space Observatory by the Institute of Geodesy of the Leibniz Universität Hannover from 2003 to 2011 and have been continued with the upgraded meter FG5X in 2014. Lantmäteriet, Gävle, with their FG5 absolute gravimeter have visited Onsala since 2007. Because small systematic errors may be inherent in each absolute gravimeter, their measuring level and a resulting bias (offset) between the instruments must be controlled over time by means of inter-comparison. From 2007 to 2014, 8 direct comparisons took place well distributed over the time span. A complete re-processing of the absolute gravity observations with the Hannover instrument has been conducted to improve the reduction of unwanted gravity effects. A new tidal model is based on continuous time series recorded with the GWR superconducting gravimeter at Onsala since 2009. The loading effect of the Kattegat is described with a varying sea bottom pressure (water and air mass load) and has been validated with the continuous gravity measurements. For the land uplift,which is a result of the still ongoing glacial isostatic adjustment in Fennoscandia, a secular gravity trend of −0.22 μGal/yr was obtained with a standard deviation of 0.17 μGal/yr. That indicates a slight uplift but is still not significantly different from zero