An automated microreactor for semi-continuous biosensor measurements.

Living bacteria or yeast cells are frequently used as bioreporters for the detection of specific chemical analytes or conditions of sample toxicity. In particular, bacteria or yeast equipped with synthetic gene circuitry that allows the production of a reliable non-cognate signal (e.g., fluorescent protein or bioluminescence) in response to a defined target make robust and flexible analytical platforms. We report here how bacterial cells expressing a fluorescence reporter ("bactosensors"), which are mostly used for batch sample analysis, can be deployed for automated semi-continuous target analysis in a single concise biochip. Escherichia coli-based bactosensor cells were continuously grown in a 13 or 50 nanoliter-volume reactor on a two-layered polydimethylsiloxane-on-glass microfluidic chip. Physiologically active cells were directed from the nl-reactor to a dedicated sample exposure area, where they were concentrated and reacted in 40 minutes with the target chemical by localized emission of the fluorescent reporter signal. We demonstrate the functioning of the bactosensor-chip by the automated detection of 50 μgarsenite-As l(-1) in water on consecutive days and after a one-week constant operation. Best induction of the bactosensors of 6-9-fold to 50 μg l(-1) was found at an apparent dilution rate of 0.12 h(-1) in the 50 nl microreactor. The bactosensor chip principle could be widely applicable to construct automated monitoring devices for a variety of targets in different environments

Search for radiative pumping lines of OH masers: I. The 34.6um absorption line towards 1612 MHz OH maser sources

The 1612 MHz hydroxyl maser in circumstellar envelopes has long been thought to be pumped by 34.6um photons. Only recently, the Infrared Space Observatory has made possible spectroscopic observations which enable the direct confirmation of this pumping mechanism in a few cases. To look for the presence of this pumping line, we have searched the Infrared Space Observatory Data Archive and found 178 spectra with data around 34.6um for 87 galactic 1612MHz masers. The analysis performed showed that the noise level and the spectral resolution of the spectra are the most important factors affecting the detection of the 34.6um absorption line. Only 5 objects from the sample (3 red supergiants and 2 galactic center sources) are found to show clear 34.6um absorption (all of them already known) while two additional objects only tentatively show this line. The 3 supergiants show similar pump rates and their masers might be purely radiatively pumped. The pump rates of OH masers in late type stars are found to be about 0.05, only 1/5 of the theoretical value of 0.25 derived by Elitzur (1992). We have also found 16 maser sources which, according to the analysis assuming Elitzur's pump rate, should show the 34.6 $\mu$m absorption line but do not. These non-detections can be tentatively explained by far-infrared photon pumping, clumpy nature of the OH masing region or a limb-filling emission effect in the OH shell.Comment: 11 pages, 8 figures, 3 table

OH-selected AGB and post-AGB objects I.Infrared and maser properties

Using 766 compact objects from a survey of the galactic Plane in the 1612-MHz OH line, new light is cast on the infrared properties of evolved stars on the TP-AGB and beyond. The usual mid-infrared selection criteria, based on IRAS colours, largely fail to distinguish early post-AGB stages. A two-colour diagram from narrower-band MSX flux densities, with bimodal distributions, provides a better tool to do the latter. Four mutually consistent selection criteria for OH-masing red PPNe are given, as well as two for early post-AGB masers and one for all post--AGB masers, including the earliest ones. All these criteria miss a group of blue, high-outflow post-AGB sources with 60-mum excess; these will be discussed in detail in Paper II. The majority of post-AGB sources show regular double-peaked spectra in the OH 1612-MHz line, with fairly low outflow velocities, although the fractions of single peaks and irregular spectra may vary with age and mass. The OH flux density shows a fairly regular relation with the stellar flux and the envelope optical depth, with the maser efficiency increasing with IRAS colour R21. The OH flux density is linearly correlated with the 60-mum flux density.Comment: 16 pages, LaTex, 22 figures, AJ (accepted

Equilibrium Models for two Samples of OH/IR Stars

Wetensch. publicatieFaculteit der Wiskunde en Natuurwetenschappe

H2O Maser Observations of Candidate Post-AGB Stars and Discovery of Three High-velocity Water Sources

We present the results of 22 GHz H_2O maser observations of a sample of 85 post-Asymptotic Giant Branch (post-AGB) candidate stars, selected on the basis of their OH 1612 MHz maser and far-infrared properties. All sources were observed with the Tidbinbilla 70-m radio telescope and 21 detections were made. 86 GHz SiO Mopra observations of a subset of the sample are also presented. Of the 21 H_2O detections, 15 are from sources that are likely to be massive AGB stars and most of these show typical, regular H_2O maser profiles. In contrast, nearly all the detections of more evolved stars exhibited high-velocity H_2O maser emission. Of the five sources seen, v223 (W43A, IRAS 18450-0148) is a well known `water-fountain' source which belongs to a small group of post-AGB stars with highly collimated, high-velocity H_2O maser emission. A second source in our sample, v270 (IRAS 18596+0315), is also known to have high-velocity emission. We report the discovery of similar emission from a further three sources, d46 (IRAS 15445-5449), d62 (IRAS 15544-5332) and b292 (IRAS 18043-2116). The source d46 is an evolved post-AGB star with highly unusual maser properties. The H_2O maser emission from d62 is probably associated with a massive star. The source b292 is a young post-AGB star that is highly likely to be a water-fountain source, with masers detected over a velocity range of 210 km s^{-1}.Comment: 47 pages, 9 figures, 4 tables, accepted by Ap

The distribution of maser stars in the inner Milky Way: the effect of a weak, rotating bar

We derive the distribution of maser stars in the inner Milky Way (MW) based on an analysis of lV-diagrams (lVd) for two samples of maser stars: 771 OH/IR stars and 363 SiO-maser stars. They are all close to the plane of the MW and have long. from -45 to +45deg. The two lVds are compared and found to be very similar. They also compare well with the lVd of interstellar CO, but there are significant differences in detail between the stellar lVds and that of the ISM. Based on the qualitative discussion we divide the lVds into seven areas. In each area we compare the number of stars observed with those predicted by an assumed set of orbits in a galactic potential. This potential is axially symmetric but a weak rotating bar has been added. We conclude that the maser stars move on almost circular orbits outside of about 3.5 kpc, but that the orbits become more and more elongated when one goes deep inside our MW. We find a strong effect of the Corotation (CR) resonance (res) at 3.3 kpc, we see a small but noticeable effect of the Outer Lindblad res at 5 kpc and no effect of the Inner Lindblad res at r=0.8 kpc. We find a set of 6 groups of orbits that together predict counts in agreement with the counts of stars observed. We then calculate the trajectory of each orbit and so find the distribution of the maser stars in the plane of the MWG. This distribution has two new (but not unexpected) features. The first is a bar-like distribution within 2 kpc from the GC outlined. These orbits explain the high-vel stars near l=0deg in the forbidden and the permitted quadrants. The second feature are two "croissant"-like voids in the distribution close to the CR radius (3.3 kpc), which are the consequence of the presence of the CR res. We find excellent agreement with an earlier reconstruction by Sevenster (1999)

Accurate OH maser positions from the SPLASH pilot region

We report on high spatial resolution observations, using the Australia Telescope Compact Array (ATCA), of ground-state OH masers. These observations were carried out toward 196 pointing centers previously identified in the Southern Parkes Large-Area Survey in Hydroxyl (SPLASH) pilot region, between Galactic longitudes of 334 and 344 and Galactic latitudes of -2 and +2. Supplementing our data with data from the MAGMO (Mapping the Galactic Magnetic field through OH masers) survey, we find maser emission toward 175 of the 196 target fields. We conclude that about half of the 21 nondetections were due to intrinsic variability. Due to the superior sensitivity of the followup ATCA observations, and the ability to resolve nearby sources into separate sites, we have identified 215 OH maser sites toward the 175 fields with detections. Among these 215 OH maser sites, 111 are new detections. After comparing the positions of these 215 maser sites to the literature, we identify 122 (57%) sites associated with evolved stars (one of which is a planetary nebula), 64 (30%) with star formation, two sites with supernova remnants, and 27 (13%) of unknown origin. The infrared colors of evolved star sites with symmetric maser profiles tend to be redder than those of evolved star sites with asymmetric maser profiles, which may indicate that symmetric sources are generally at an earlier evolutionary stage. © 2016. The American Astronomical Society. All rights reserved

86 GHz SiO maser survey of late-type stars in the Inner Galaxy II. Infrared photometry of the SiO Target Stars

We present a compilation and study of DENIS, 2MASS, ISOGAL, MSX and IRAS 1--25 micron photometry for a sample of 441 late-type stars in the inner Galaxy, which we previously searched for 86 GHz SiO maser emission (Messineo et al.2002). The comparison of the DENIS and 2MASS J and Ks magnitudes shows that most of the SiO targets are indeed variable stars. The MSX colours and the IRAS [12]-[25] colour of our SiO targets are consistent with those of Mira type stars with dust silicate feature at 9.7 micron feature in emission, indicating only a moderate mass-loss rate.Comment: 16 pages, 17 figures. Accepted for publication in A&

Molecules in bipolar proto-planetary nebulae

Two bipolar proto-planetary nebulae, IRAS16594-4656 and IRAS17150-3224, have been detected in various molecular lines, namely CO, ^{13}CO, HCN and CN, and remain undetected in several other species. CO(J=2-1) and CO(J=3-2) line profiles are compared to new spectra of similar PPN candidates, previously undetected in CO(J=2-1): CPD-53^{o}5736, IRAS17106-3046, IRAS17245-3951 and IRAS17441-2411. CO(J=2-1) maps of IRAS16594-4656 and IRAS17150-3224 show that both PPNe have little separation between blue, centre and red-shifted emission, and also that the CO(J=2-1) emission is of a similar size to the telescope beam. Fractional abundances of all detected molecules (except CO) are calculated using the results of CO line modelling and a simple photodissociation model. For those species not detected, upper limits are derived. Comparisons between these fractional abundances and those of other PPNe show that IRAS16594-4656 and IRAS17150-3224 are quite under-abundant when compared to molecule-rich sources like CRL618, CRL2688 and OH231.8+4.2. As a reason for this deficit, the difference in circumstellar envelope/torus density between the molecule-rich sources and the molecule-poor sources is proposed, and supported by a chemical model which follows the transition of a circumstellar envelope through the AGB phase and into the PPN phase of evolution. The model includes the effects of UV radiation, cosmic rays and also X-rays. Finally, the post-AGB ages of these two objects (200-400 yr) are estimated using CN/HCN and HCN/CO ratios and both ages are found to be in agreement with previous figures cited in the literature, IRAS17150-3224 being the younger of the two PPNe.Comment: Accepted by Astronomy & Astrophysics. 21 pages, 11 figure