2,553 research outputs found
STIM map: detection map for exoplanets imaging beyond asymptotic Gaussian residual speckle noise
Direct imaging of exoplanets is a challenging task as it requires to reach a
high contrast at very close separation to the star. Today, the main limitation
in the high-contrast images is the quasi-static speckles that are created by
residual instrumental aberrations. They have the same angular size as planetary
companions and are often brighter, hence hindering our capability to detect
exoplanets. Dedicated observation strategies and signal processing techniques
are necessary to disentangle these speckles from planetary signals. The output
of these methods is a detection map in which the value of each pixel is related
to a probability of presence of a planetary signal. The detection map found in
the literature relies on the assumption that the residual noise is Gaussian.
However, this is known to lead to higher false positive rates, especially close
to the star. In this paper, we re-visit the notion of detection map by
analyzing the speckle noise distribution, namely the Modified Rician
distribution. We use non-asymptotic analysis of the sum of random variables to
show that the tail of the distribution of the residual noise decays as an
exponential distribution, hence explaining the high false detection rate
obtained with the Gaussian assumption. From this analysis, we introduce a novel
time domain detection map and we demonstrate its capabilities and the relevance
of our approach through experiments on real data. We also provide an empirical
rule to determine detection threshold providing a good trade off between true
positive and false positive rates for exoplanet detection
Carbon-fiber tips for scanning probe microscopes and molecular electronics experiments
We fabricate and characterize carbon-fiber tips for their use in combined
scanning tunneling and force microscopy based on piezoelectric quartz tuning
fork force sensors. An electrochemical fabrication procedure to etch the tips
is used to yield reproducible sub-100-nm apex. We also study electron transport
through single-molecule junctions formed by a single octanethiol molecule
bonded by the thiol anchoring group to a gold electrode and linked to a carbon
tip by the methyl group. We observe the presence of conductance plateaus during
the stretching of the molecular bridge, which is the signature of the formation
of a molecular junction.Comment: Conference Proceeding (Trends in NanoTechnology 2011, Tenerife
SPAIN); Nanoscale Research Letters, (2012) 7:25
Differential cognitive impairment for diverse forms of multiple sclerosis
BACKGROUND: Cognitive impairment is a common feature in multiple sclerosis (MS) patients and occurs in 60% of all cases. Unfortunately, neurological examination does not always agree with the neuropsychological evaluation in determining the cognitive profile of the patient. On the other hand, psychophysiological techniques such as event-related potentials (ERPs) can help in evaluating cognitive impairment in different pathologies. Behavioural responses and EEG signals were recorded during the experiment in three experimental groups: 1) a relapsing-remitting group (RRMS), 2) a benign multiple sclerosis group (BMS) and 3) a Control group. The paradigm employed was a spatial attention task with central cues (Posner experiment). The main aim was to observe the differences in the performance (behavioural variables) and in the latency and amplitude of the ERP components among these groups. RESULTS: Our data indicate that both MS groups showed poorer task performance (longer reaction times and lower percentage of correct responses), a latency delay for the N1 and P300 component, and a different amplitude for the frontal N1. Moreover, the deficit in the BMS group, indexed by behavioural and pyschophysiological variables, was more pronounced compared to the RRMS group. CONCLUSION: The present results suggest a cognitive impairment in the information processing in all of these patients. Comparing both pathological groups, cognitive impairment was more accentuated in the BMS group compared to the RMSS group. This suggests a silent deterioration of cognitive skills for the BMS that is not usually treated with pharmacological or neuropsychological therapy
Multidrug transporter MRP4/ABCC4 as a key determinant of pancreatic cancer aggressiveness
Recent findings show that MRP4 is critical for pancreatic ductal adenocarcinoma (PDAC) cell proliferation. Nevertheless, the significance of MRP4 protein levels and function in PDAC progression is still unclear. The aim of this study was to determine the role of MRP4 in PDAC tumor aggressiveness. Bioinformatic studies revealed that PDAC samples show higher MRP4 transcript levels compared to normal adjacent pancreatic tissue and circulating tumor cells express higher levels of MRP4 than primary tumors. Also, high levels of MRP4 are typical of high-grade PDAC cell lines and associate with an epithelial-mesenchymal phenotype. Moreover, PDAC patients with high levels of MRP4 depict dysregulation of pathways associated with migration, chemotaxis and cell adhesion. Silencing MRP4 in PANC1 cells reduced tumorigenicity and tumor growth and impaired cell migration. Transcriptomic analysis revealed that MRP4 silencing alters PANC1 gene expression, mainly dysregulating pathways related to cell-to-cell interactions and focal adhesion. Contrarily, MRP4 overexpression significantly increased BxPC-3 growth rate, produced a switch in the expression of EMT markers, and enhanced experimental metastatic incidence. Altogether, our results indicate that MRP4 is associated with a more aggressive phenotype in PDAC, boosting pancreatic tumorigenesis and metastatic capacity, which could finally determine a fast tumor progression in PDAC patients.Fil: Sahores, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Carozzo, A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: May, M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Gomez, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Di Siervi, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: de Sousa Serro, Maximiliano Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Yaneff, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Rodriguez Gonzalez, Angela Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Shayo, Carina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Davio, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentin
The VORTEX project: first results and perspectives
(abridged) Vortex coronagraphs are among the most promising solutions to
perform high contrast imaging at small angular separations. They feature a very
small inner working angle, a clear 360 degree discovery space, have
demonstrated very high contrast capabilities, are easy to implement on
high-contrast imaging instruments, and have already been extensively tested on
the sky. Since 2005, we have been designing, developing and testing an
implementation of the charge-2 vector vortex phase mask based on concentric
subwavelength gratings, referred to as the Annular Groove Phase Mask (AGPM).
Science-grade mid-infrared AGPMs were produced in 2012 for the first time,
using plasma etching on synthetic diamond substrates. They have been validated
on a coronagraphic test bench, showing broadband peak rejection up to 500:1 in
the L band, which translates into a raw contrast of about at
. Three of them have now been installed on world-leading
diffraction-limited infrared cameras (VLT/NACO, VLT/VISIR and LBT/LMIRCam).
During the science verification observations with our L-band AGPM on NACO, we
observed the beta Pictoris system and obtained unprecedented sensitivity limits
to planetary companions down to the diffraction limit (). More recently,
we obtained new images of the HR 8799 system at L band during the AGPM first
light on LMIRCam. After reviewing these first results obtained with
mid-infrared AGPMs, we will discuss the short- and mid-term goals of the
on-going VORTEX project, which aims to improve the performance of our vortex
phase masks for future applications on second-generation high-contrast imagers
and on future extremely large telescopes (ELTs).Comment: To appear in SPIE proceedings vol. 914
Improving Cr (VI) Extraction through Electrodialysis
A laboratory study has been carried out to determine the feasibility of in situ remediation of chromium (VI)
contaminated soil using electrodialysis. In a classic setup, this technique implies the application of a low intensity direct
current to the soil, which is separated from the electrode compartments by ion-exchange membranes. If the pollutants
are ionic compounds, they can be forced to migrate to the oppositely charged electrode by electro-migration.
Membranes selectively impede the flow of ions in the electrode compartments back to the soil. If a metal species is
naturally present as an anion, mobilization from the soil at alkaline pH can be realized and, at the same time, the
mobilization of other metal cations that occur at low pH can be minimized.
Experiments have been carried out with clayey soils (kaolinite clay and soil clay mixtures) that have been
characterized and then contaminated by mixing with a potassium dichromate solution for several days. Initial Cr (VI)
content ranges from 500 to 4000 mg/kg. Treatment tests were carried out in an acrylic laboratory cells consisting of a
central soil compartment and two electrode compartments located at both ends of the column. Dimensionally stable
titanium electrodes coated with mixed metal oxides were placed in the electrode compartments. 0.01M Na2SO4
electrolytes were recirculated through them from two 1-liter deposits using a peristaltic pump. Two commercial ion
exchange membranes separated the anolyte and catholyte compartments from the soil in the standard configuration. A
programmable DC: power supply was connected to the electrodes and a computer for data acquisition.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
The authors acknowledge the financial support from the "Plan Propio de Investigación de la Universidad de Málaga" with project numbers PPIT.UMA.D1; PPIT.UMA.B1.2017/20 and PPIT.UMA.B5.2018/17. This work has also received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 778045
Three years of harvest with the vector vortex coronagraph in the thermal infrared
For several years, we have been developing vortex phase masks based on
sub-wavelength gratings, known as Annular Groove Phase Masks. Etched onto
diamond substrates, these AGPMs are currently designed to be used in the
thermal infrared (ranging from 3 to 13 {\mu}m). Our AGPMs were first installed
on VLT/NACO and VLT/VISIR in 2012, followed by LBT/LMIRCam in 2013 and
Keck/NIRC2 in 2015. In this paper, we review the development, commissioning,
on-sky performance, and early scientific results of these new coronagraphic
modes and report on the lessons learned. We conclude with perspectives for
future developments and applications.Comment: To appear in SPIE proceedings vol. 990
Neutrino Masses and Mixings from Supersymmetry with Bilinear R--Parity Violation: A Theory for Solar and Atmospheric Neutrino Oscillations
The simplest unified extension of the MSSM with bi-linear R--Parity violation
naturally predicts a hierarchical neutrino mass spectrum, in which one neutrino
acquires mass by mixing with neutralinos, while the other two get mass
radiatively. We have performed a full one-loop calculation of the
neutralino-neutrino mass matrix in the bi-linear \rp MSSM, taking special care
to achieve a manifestly gauge invariant calculation. Moreover we have performed
the renormalization of the heaviest neutrino, needed in order to get meaningful
results. The atmospheric mass scale and maximal mixing angle arise from
tree-level physics, while solar neutrino scale and oscillations follow from
calculable one-loop corrections. If universal supergravity assumptions are made
on the soft-supersymmetry breaking terms then the atmospheric scale is
calculable as a function of a single \rp violating parameter by the
renormalization group evolution due to the non-zero bottom quark Yukawa
coupling. The solar neutrino problem must be accounted for by the small mixing
angle (SMA) MSW solution. If these assumptions are relaxed then one can
implement large mixing angle solutions, either MSW or just-so. The theory
predicts the lightest supersymmetic particle (LSP) decay to be observable at
high-energy colliders, despite the smallness of neutrino masses indicated by
experiment. This provides an independent way to test this solution of the
atmospheric and solar neutrino anomalies.Comment: 46 pages, references added + several misprints correcte
Differential Role of Human Choline Kinase α and β Enzymes in Lipid Metabolism: Implications in Cancer Onset and Treatment
11 pages, 6 figures, 1 table.Background
The Kennedy pathway generates phosphocoline and phosphoethanolamine through its two branches. Choline Kinase (ChoK) is the first enzyme of the Kennedy branch of synthesis of 1phosphocholine, the major component of the plasma membrane. ChoK family of proteins is composed by ChoKα and ChoKβ isoforms, the first one with two different variants of splicing. Recently ChoKα has been implicated in the carcinogenic process, since it is over-expressed in a variety of human cancers. However, no evidence for a role of ChoKβ in carcinogenesis has been reported.
Methodology/Principal Findings
Here we compare the in vitro and in vivo properties of ChoKα1 and ChoKβ in lipid metabolism, and their potential role in carcinogenesis. Both ChoKα1 and ChoKβ showed choline and ethanolamine kinase activities when assayed in cell extracts, though with different affinity for their substrates. However, they behave differentially when overexpressed in whole cells. Whereas ChoKβ display an ethanolamine kinase role, ChoKα1 present a dual choline/ethanolamine kinase role, suggesting the involvement of each ChoK isoform in distinct biochemical pathways under in vivo conditions. In addition, while overexpression of ChoKα1 is oncogenic when overexpressed in HEK293T or MDCK cells, ChoKβ overexpression is not sufficient to induce in vitro cell transformation nor in vivo tumor growth. Furthermore, a significant upregulation of ChoKα1 mRNA levels in a panel of breast and lung cancer cell lines was found, but no changes in ChoKβ mRNA levels were observed. Finally, MN58b, a previously described potent inhibitor of ChoK with in vivo antitumoral activity, shows more than 20-fold higher efficiency towards ChoKα1 than ChoKβ.
Conclusion/Significance
This study represents the first evidence of the distinct metabolic role of ChoKα and ChoKβ isoforms, suggesting different physiological roles and implications in human carcinogenesis. These findings constitute a step forward in the design of an antitumoral strategy based on ChoK inhibition.This work has been supported by grants to JCL from Comunidad de Madrid (GR-SAL-0821-2004), Ministerio de Ciencia e Innovación (SAF2008-03750, RD06/0020/0016), Fundación Mutua Madrileña, and by a grant to ARM from Fundación Mutua Madrileña.Peer reviewe
Hypothalamic AMPK-ER Stress-JNK1 Axis Mediates the Central Actions of Thyroid Hormones on Energy Balance
Thyroid hormones (THs) act in the brain to modulate energy balance. We show that central triiodothyronine (T3) regulates de novo lipogenesis in liver and lipid oxidation in brown adipose tissue (BAT) through the parasympathetic (PSNS) and sympathetic nervous system (SNS), respectively. Central T3 promotes hepatic lipogenesis with parallel stimulation of the thermogenic program in BAT. The action of T3 depends on AMP-activated protein kinase (AMPK)-induced regulation of two signaling pathways in the ventromedial nucleus of the hypothalamus (VMH): decreased ceramide-induced endoplasmic reticulum(ER) stress, which promotes BAT thermogenesis, and increased c-Jun N-terminal kinase (JNK) activation, which controls hepatic lipid metabolism. Of note, ablation of AMPK alpha 1 in steroidogenic factor 1 (SF1) neurons of the VMH fully recapitulated the effect of central T3, pointing to this population in mediating the effect of central THs on metabolism. Overall, these findings uncover the underlying pathways through which central T3 modulates peripheral metabolism.Peer reviewe
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