Óleos essencias e fungos no manejo de mosca-das-frutas(Ceratitis capilata) (WIED, 1824) e influência na qualidade de frutos da goiabeira.

Brazil is one of the world's largest fruit producers, with a production of more than 41 million tons per year. However, several pests are responsible for causing negative impacts on Brazilian fruit crops, notably the damage caused by fruit fly Ceratitis capitata (WIED, 1824). Females lay their eggs in the fruits, which will be a food resource for the developing larvae, causing significant damage to their quality. New pest control techniques, such as the use of natural products, have been developed, mainly for their effectiveness and degradability. This work consists of two scientific articles. The first article evaluates the insecticidal action of essential oils and entomopathogenic fungi on different stages of fruit fly development, the second; verifies the repellent power of oils on the adult phase of insects and evaluates the chemical composition of guava fruits Psidium guajava L. variety "Paluma" treated with essential oils. The research was conducted at the Invertebrate Zoology Laboratory belonging to the Center of Agrarian Sciences of the Federal University of Paraíba. Citronella, Carnation and Copaíba essential oils and isolates of Metarhizium anisopliae and Beauveria bassiana were used. Topical applications of 2.0 μL of each product/concentration with micropipette aid were performed on 1st, 2nd and 3rd instar larvae and pupae. There was no significant difference between the different types of oils (citronella, clove and copaíba) on larval and pupal mortality when the larvae were treated in the 1st and 2nd instar. When treated in the 3rd instar, there was a significant effect on pupal mortality. The increase in citronella oil concentration continuously increased the repelling potential. The application of essential oils did not negatively influence fruit quality.O Brasil é um dos maiores produtores mundias de frutas, com uma produção da ordem de mais de 41 milhões de toneladas por ano. Contudo, diversas pragas são responsáveis por causarem impactos negativos à fruticultura brasileira, merecendo destaque os prejuízos causados pela mosca-das-frutas Ceratitis capitata (WIED, 1824). As fêmeas depositam seus ovos nos frutos, que serão recurso alimentar para as larvas em desenvolvimento, causando danos significativos na qualidade destes. Novas técnicas de controle de pragas, como o uso de produtos naturais, vêm sendo desenvolvidos, principalmente por sua eficácia e degradabilidade. Este trabalho é composto por dois artigos científicos. O primeiro artigo avalia a ação inseticida de óleos esseciais e fungos entomopatogênicos sobre diferentes estágios de desenvolvimento da mosca-das-frutas, o segundo; verifica o poder de repelência dos óleos sobre a fase adulta dos insetos e avalia a composição química dos frutos de goiabaira Psidium guajava L. variedade “Paluma” tratados com os óleos essenciais. A pesquisa foi conduzida no Laboratório de Zoologia de Invertebrados pertencente ao Centro de Ciências Agrárias da Universidade Federal da Paraíba. Foram utilizados óleos essenciais de Citronela, Cravo e Copaíba e isolados das espécies Metarhizium anisopliae e Beauveria bassiana. Efetuaram-se aplicações tópicas de 2,0 μL de cada produto/concentração com auxílio de micropipeta em larvas de 1°, 2° e 3° ínstar e pupas. Não houve diferença significativa entre os diferentes tipos de óleos (citronela, cravo e copaíba) sobre a mortalidade de larvas e pupas, quando as larvas foram tratadas nos 1° e 2° ínstar. Quando tratadas no 3° ínstar, houve efeito significativo sobre a mortalidade das pupas. O aumento na concentração do óleo de citronela aumentou continuamente o potencial de repelência. A aplicação dos óleos essenciais não influenciou negativamente na qualidade dos frutos

DINÂMICA POPULACIONAL DE MOSCAS-DAS-FRUTAS (Diptera: Tephritidae) E PARASITOIDES EM POMARES DOMÉSTICOS NOS MUNICÍPIOS DE BANANEIRAS E BORBOREMA – PB

The fruit industry in Brazil is considered one of the largest in the world, standing out as the third largest fruit producer, with an estimated production of more than 42 million tons. However, the production destined for the foreign market is very low, due to the low technological level used in the cultivation of the fruit trees added to the phytosanitary problems that directly reflect in the quality of the fruits. Infestation by fruit flies (Diptera: Tephritidae) is considered to be the major obstacle in the production, commercialization and export of fruit, characterizing itself as one of the biggest pests in the country. The aim of this study was to evaluate quantitatively the biodiversity of Tefritídeos and the occurrence of parasitoids associated to domestic orchards in the municipalities of Bananeiras and Borborema - PB. The fruits were obtained through biweekly collections in domestic orchards, preferentially picking ripe fruits or at the beginning of maturation, differentiating fruits of soil and plant. The monitoring of adult fruit flies was carried out using traps constructed from two-liter pet bottles containing as an attractive food 300 ml of aqueous solution of hydrolyzed protein at 5%. A total of 1,137 fruit flies were captured in the traps and fruits, seven of them belonging to the genus Anastrepha: A. fraterculus, A sororcula, A. obliqua, A. distincta, A. antunesi, A. zenildae and A. Barbiellinii and one belonging to the genus Ceratitis: C. capitata. A. fraterculus was the most dominant and constant species in both cities, fruit availability was the factor that determined the population peaks and the level of natural parasitism was considered relatively low.O setor da fruticultura no Brasil é considerado um dos maiores do mundo, destacando-se como o terceiro maior produtor de frutas, com produção estimada de mais de 42 milhões de toneladas. Contudo, a produção destinada para o mercado externo é muito reduzida, devido ao baixo nível tecnológico utilizado no cultivo das fruteiras somado aos problemas fitossanitários que refletem diretamente na qualidade dos frutos. A infestação por moscas-das-frutas (Diptera: Tephritidae) é considerado o maior entrave na produção, comercialização e exportação de frutíferas, caracterizando-se como uma das maiores pragas do país. O intuito desse trabalho foi avaliar quantitativamente a biodiversidade dos Tefritídeos e a ocorrência de parasitóides associados a pomares domésticos nos municípios de Bananeiras e Borborema – PB. Os frutos foram obtidos através de coletas quinzenais em pomares domésticos coletando-se preferencialmente frutos maduros ou em início de maturação, diferenciando frutos de solo e da planta. O monitoramento dos adultos de moscas-das-frutas foi realizado com auxílio de armadilhas construídas a partir de garrafas pet de dois litros, contendo como atrativo alimentar 300 ml de solução aquosa de proteína hidrolisada a 5%. Foram capturadas nas armadilhas e nos frutos um total de 1.137 moscas-das-frutas, dessas, sete pertencentes ao gênero Anastrepha: A. fraterculus, A sororcula, A. obliqua, A. distincta, A. antunesi, A. zenildae e A. barbiellinii e uma pertencente ao gênero Ceratitis: C. capitata. A A. fraterculus foi a espécie mais dominante e constante em ambas as cidades, a disponibilidade dos frutos foi o fator que determinou os picos populacionais e o nível de parasitismo natural foi considerado relativamente baixo

Productividad de la yuca en respuesta al espaciamiento y la fertilización con potasio de la cubierta en Brejo Paraibano

O presente trabalho objetivou analisar o desempenho produtivo da mandioca sob diferentes espaçamentos e a presença e ausência de adubação potássica de cobertura. O experimento foi conduzido em área experimental no município de Areia, Paraíba. Utilizou-se a variedade SRT 1105 roxinha, sendo empregado delineamento de blocos casualisados, com fatorial 3 x 2 (três espaçamentos com e sem adubação potássica em cobertura) em quatro repetições. As avaliações do desenvolvimento vegetativo da cultura foram feitas no momento da colheita, na qual foram mensurados a altura de planta (cm) e diâmetro do caule (cm). Para as raízes foram analisados o comprimento (cm), diâmetro (cm), peso individual (Kg) e a produtividade (t ha-1). Os dados foram submetidos a análise de variância e as médias comparadas pelo teste de Tukey a 5% de probabilidade. Observou-se efeito significativo a 5%, do espaçamento sob a altura de plantas (AP). Para a interação espaçamento versus Potássio, encontrou-se efeito significativo a 5% também para a altura de plantas (AP). Para as demais variáveis não se observou efeito significativo de nenhuma das fontes de variação. A aplicação de Potássio aos 180 dias e os espaçamentos de 0,7;10 e 1,3m não promoveram incremento de produtividade para a variedade Roxinha

Human genome meeting 2016 : Houston, TX, USA. 28 February - 2 March 2016

: O1 The metabolomics approach to autism: identification of biomarkers for early detection of autism spectrum disorder A. K. Srivastava, Y. Wang, R. Huang, C. Skinner, T. Thompson, L. Pollard, T. Wood, F. Luo, R. Stevenson O2 Phenome-wide association study for smoking- and drinking-associated genes in 26,394 American women with African, Asian, European, and Hispanic descents R. Polimanti, J. Gelernter O3 Effects of prenatal environment, genotype and DNA methylation on birth weight and subsequent postnatal outcomes: findings from GUSTO, an Asian birth cohort X. Lin, I. Y. Lim, Y. Wu, A. L. Teh, L. Chen, I. M. Aris, S. E. Soh, M. T. Tint, J. L. MacIsaac, F. Yap, K. Kwek, S. M. Saw, M. S. Kobor, M. J. Meaney, K. M. Godfrey, Y. S. Chong, J. D. Holbrook, Y. S. Lee, P. D. Gluckman, N. Karnani, GUSTO study group O4 High-throughput identification of specific qt interval modulating enhancers at the SCN5A locus A. Kapoor, D. Lee, A. Chakravarti O5 Identification of extracellular matrix components inducing cancer cell migration in the supernatant of cultivated mesenchymal stem cells C. Maercker, F. Graf, M. Boutros O6 Single cell allele specific expression (ASE) IN T21 and common trisomies: a novel approach to understand DOWN syndrome and other aneuploidies G. Stamoulis, F. Santoni, P. Makrythanasis, A. Letourneau, M. Guipponi, N. Panousis, M. Garieri, P. Ribaux, E. Falconnet, C. Borel, S. E. Antonarakis O7 Role of microRNA in LCL to IPSC reprogramming S. Kumar, J. Curran, J. Blangero O8 Multiple enhancer variants disrupt gene regulatory network in Hirschsprung disease S. Chatterjee, A. Kapoor, J. Akiyama, D. Auer, C. Berrios, L. Pennacchio, A. Chakravarti O9 Metabolomic profiling for the diagnosis of neurometabolic disorders T. R. Donti, G. Cappuccio, M. Miller, P. Atwal, A. Kennedy, A. Cardon, C. Bacino, L. Emrick, J. Hertecant, F. Baumer, B. Porter, M. Bainbridge, P. Bonnen, B. Graham, R. Sutton, Q. Sun, S. Elsea O10 A novel causal methylation network approach to Alzheimer’s disease Z. Hu, P. Wang, Y. Zhu, J. Zhao, M. Xiong, David A Bennett O11 A microRNA signature identifies subtypes of triple-negative breast cancer and reveals MIR-342-3P as regulator of a lactate metabolic pathway A. Hidalgo-Miranda, S. Romero-Cordoba, S. Rodriguez-Cuevas, R. Rebollar-Vega, E. Tagliabue, M. Iorio, E. D’Ippolito, S. Baroni O12 Transcriptome analysis identifies genes, enhancer RNAs and repetitive elements that are recurrently deregulated across multiple cancer types B. Kaczkowski, Y. Tanaka, H. Kawaji, A. Sandelin, R. Andersson, M. Itoh, T. Lassmann, the FANTOM5 consortium, Y. Hayashizaki, P. Carninci, A. R. R. Forrest O13 Elevated mutation and widespread loss of constraint at regulatory and architectural binding sites across 11 tumour types C. A. Semple O14 Exome sequencing provides evidence of pathogenicity for genes implicated in colorectal cancer E. A. Rosenthal, B. Shirts, L. Amendola, C. Gallego, M. Horike-Pyne, A. Burt, P. Robertson, P. Beyers, C. Nefcy, D. Veenstra, F. Hisama, R. Bennett, M. Dorschner, D. Nickerson, J. Smith, K. Patterson, D. Crosslin, R. Nassir, N. Zubair, T. Harrison, U. Peters, G. Jarvik, NHLBI GO Exome Sequencing Project O15 The tandem duplicator phenotype as a distinct genomic configuration in cancer F. Menghi, K. Inaki, X. Woo, P. Kumar, K. Grzeda, A. Malhotra, H. Kim, D. Ucar, P. Shreckengast, K. Karuturi, J. Keck, J. Chuang, E. T. Liu O16 Modeling genetic interactions associated with molecular subtypes of breast cancer B. Ji, A. Tyler, G. Ananda, G. Carter O17 Recurrent somatic mutation in the MYC associated factor X in brain tumors H. Nikbakht, M. Montagne, M. Zeinieh, A. Harutyunyan, M. Mcconechy, N. Jabado, P. Lavigne, J. Majewski O18 Predictive biomarkers to metastatic pancreatic cancer treatment J. B. Goldstein, M. Overman, G. Varadhachary, R. Shroff, R. Wolff, M. Javle, A. Futreal, D. Fogelman O19 DDIT4 gene expression as a prognostic marker in several malignant tumors L. Bravo, W. Fajardo, H. Gomez, C. Castaneda, C. Rolfo, J. A. Pinto O20 Spatial organization of the genome and genomic alterations in human cancers K. C. Akdemir, L. Chin, A. Futreal, ICGC PCAWG Structural Alterations Group O21 Landscape of targeted therapies in solid tumors S. Patterson, C. Statz, S. Mockus O22 Genomic analysis reveals novel drivers and progression pathways in skin basal cell carcinoma S. N. Nikolaev, X. I. Bonilla, L. Parmentier, B. King, F. Bezrukov, G. Kaya, V. Zoete, V. Seplyarskiy, H. Sharpe, T. McKee, A. Letourneau, P. Ribaux, K. Popadin, N. Basset-Seguin, R. Ben Chaabene, F. Santoni, M. Andrianova, M. Guipponi, M. Garieri, C. Verdan, K. Grosdemange, O. Sumara, M. Eilers, I. Aifantis, O. Michielin, F. de Sauvage, S. Antonarakis O23 Identification of differential biomarkers of hepatocellular carcinoma and cholangiocarcinoma via transcriptome microarray meta-analysis S. Likhitrattanapisal O24 Clinical validity and actionability of multigene tests for hereditary cancers in a large multi-center study S. Lincoln, A. Kurian, A. Desmond, S. Yang, Y. Kobayashi, J. Ford, L. Ellisen O25 Correlation with tumor ploidy status is essential for correct determination of genome-wide copy number changes by SNP array T. L. Peters, K. R. Alvarez, E. F. Hollingsworth, D. H. Lopez-Terrada O26 Nanochannel based next-generation mapping for interrogation of clinically relevant structural variation A. Hastie, Z. Dzakula, A. W. Pang, E. T. Lam, T. Anantharaman, M. Saghbini, H. Cao, BioNano Genomics O27 Mutation spectrum in a pulmonary arterial hypertension (PAH) cohort and identification of associated truncating mutations in TBX4 C. Gonzaga-Jauregui, L. Ma, A. King, E. Berman Rosenzweig, U. Krishnan, J. G. Reid, J. D. Overton, F. Dewey, W. K. Chung O28 NORTH CAROLINA macular dystrophy (MCDR1): mutations found affecting PRDM13 K. Small, A. DeLuca, F. Cremers, R. A. Lewis, V. Puech, B. Bakall, R. Silva-Garcia, K. Rohrschneider, M. Leys, F. S. Shaya, E. Stone O29 PhenoDB and genematcher, solving unsolved whole exome sequencing data N. L. Sobreira, F. Schiettecatte, H. Ling, E. Pugh, D. Witmer, K. Hetrick, P. Zhang, K. Doheny, D. Valle, A. Hamosh O30 Baylor-Johns Hopkins Center for Mendelian genomics: a four year review S. N. Jhangiani, Z. Coban Akdemir, M. N. Bainbridge, W. Charng, W. Wiszniewski, T. Gambin, E. Karaca, Y. Bayram, M. K. Eldomery, J. Posey, H. Doddapaneni, J. Hu, V. R. Sutton, D. M. Muzny, E. A. Boerwinkle, D. Valle, J. R. Lupski, R. A. Gibbs O31 Using read overlap assembly to accurately identify structural genetic differences in an ashkenazi jewish trio S. Shekar, W. Salerno, A. English, A. Mangubat, J. Bruestle O32 Legal interoperability: a sine qua non for international data sharing A. Thorogood, B. M. Knoppers, Global Alliance for Genomics and Health - Regulatory and Ethics Working Group O33 High throughput screening platform of competent sineups: that can enhance translation activities of therapeutic target H. Takahashi, K. R. Nitta, A. Kozhuharova, A. M. Suzuki, H. Sharma, D. Cotella, C. Santoro, S. Zucchelli, S. Gustincich, P. Carninci O34 The undiagnosed diseases network international (UDNI): clinical and laboratory research to meet patient needs J. J. Mulvihill, G. Baynam, W. Gahl, S. C. Groft, K. Kosaki, P. Lasko, B. Melegh, D. Taruscio O36 Performance of computational algorithms in pathogenicity predictions for activating variants in oncogenes versus loss of function mutations in tumor suppressor genes R. Ghosh, S. Plon O37 Identification and electronic health record incorporation of clinically actionable pharmacogenomic variants using prospective targeted sequencing S. Scherer, X. Qin, R. Sanghvi, K. Walker, T. Chiang, D. Muzny, L. Wang, J. Black, E. Boerwinkle, R. Weinshilboum, R. Gibbs O38 Melanoma reprogramming state correlates with response to CTLA-4 blockade in metastatic melanoma T. Karpinets, T. Calderone, K. Wani, X. Yu, C. Creasy, C. Haymaker, M. Forget, V. Nanda, J. Roszik, J. Wargo, L. Haydu, X. Song, A. Lazar, J. Gershenwald, M. Davies, C. Bernatchez, J. Zhang, A. Futreal, S. Woodman O39 Data-driven refinement of complex disease classification from integration of heterogeneous functional genomics data in GeneWeaver E. J. Chesler, T. Reynolds, J. A. Bubier, C. Phillips, M. A. Langston, E. J. Baker O40 A general statistic framework for genome-based disease risk prediction M. Xiong, L. Ma, N. Lin, C. Amos O41 Integrative large-scale causal network analysis of imaging and genomic data and its application in schizophrenia studies N. Lin, P. Wang, Y. Zhu, J. Zhao, V. Calhoun, M. Xiong O42 Big data and NGS data analysis: the cloud to the rescue O. Dobretsberger, M. Egger, F. Leimgruber O43 Cpipe: a convergent clinical exome pipeline specialised for targeted sequencing S. Sadedin, A. Oshlack, Melbourne Genomics Health Alliance O44 A Bayesian classification of biomedical images using feature extraction from deep neural networks implemented on lung cancer data V. A. A. Antonio, N. Ono, Clark Kendrick C. Go O45 MAV-SEQ: an interactive platform for the Management, Analysis, and Visualization of sequence data Z. Ahmed, M. Bolisetty, S. Zeeshan, E. Anguiano, D. Ucar O47 Allele specific enhancer in EPAS1 intronic regions may contribute to high altitude adaptation of Tibetans C. Zeng, J. Shao O48 Nanochannel based next-generation mapping for structural variation detection and comparison in trios and populations H. Cao, A. Hastie, A. W. Pang, E. T. Lam, T. Liang, K. Pham, M. Saghbini, Z. Dzakula O49 Archaic introgression in indigenous populations of Malaysia revealed by whole genome sequencing Y. Chee-Wei, L. Dongsheng, W. Lai-Ping, D. Lian, R. O. Twee Hee, Y. Yunus, F. Aghakhanian, S. S. Mokhtar, C. V. Lok-Yung, J. Bhak, M. Phipps, X. Shuhua, T. Yik-Ying, V. Kumar, H. Boon-Peng O50 Breast and ovarian cancer prevention: is it time for population-based mutation screening of high risk genes? I. Campbell, M.-A. Young, P. James, Lifepool O53 Comprehensive coverage from low DNA input using novel NGS library preparation methods for WGS and WGBS C. Schumacher, S. Sandhu, T. Harkins, V. Makarov O54 Methods for large scale construction of robust PCR-free libraries for sequencing on Illumina HiSeqX platform H. DoddapaneniR. Glenn, Z. Momin, B. Dilrukshi, H. Chao, Q. Meng, B. Gudenkauf, R. Kshitij, J. Jayaseelan, C. Nessner, S. Lee, K. Blankenberg, L. Lewis, J. Hu, Y. Han, H. Dinh, S. Jireh, K. Walker, E. Boerwinkle, D. Muzny, R. Gibbs O55 Rapid capture methods for clinical sequencing J. Hu, K. Walker, C. Buhay, X. Liu, Q. Wang, R. Sanghvi, H. Doddapaneni, Y. Ding, N. Veeraraghavan, Y. Yang, E. Boerwinkle, A. L. Beaudet, C. M. Eng, D. M. Muzny, R. A. Gibbs O56 A diploid personal human genome model for better genomes from diverse sequence data K. C. C. Worley, Y. Liu, D. S. T. Hughes, S. C. Murali, R. A. Harris, A. C. English, X. Qin, O. A. Hampton, P. Larsen, C. Beck, Y. Han, M. Wang, H. Doddapaneni, C. L. Kovar, W. J. Salerno, A. Yoder, S. Richards, J. Rogers, J. R. Lupski, D. M. Muzny, R. A. Gibbs O57 Development of PacBio long range capture for detection of pathogenic structural variants Q. Meng, M. Bainbridge, M. Wang, H. Doddapaneni, Y. Han, D. Muzny, R. Gibbs O58 Rhesus macaques exhibit more non-synonymous variation but greater impact of purifying selection than humans R. A. Harris, M. Raveenedran, C. Xue, M. Dahdouli, L. Cox, G. Fan, B. Ferguson, J. Hovarth, Z. Johnson, S. Kanthaswamy, M. Kubisch, M. Platt, D. Smith, E. Vallender, R. Wiseman, X. Liu, J. Below, D. Muzny, R. Gibbs, F. Yu, J. Rogers O59 Assessing RNA structure disruption induced by single-nucleotide variation J. Lin, Y. Zhang, Z. Ouyang P1 A meta-analysis of genome-wide association studies of mitochondrial dna copy number A. Moore, Z. Wang, J. Hofmann, M. Purdue, R. Stolzenberg-Solomon, S. Weinstein, D. Albanes, C.-S. Liu, W.-L. Cheng, T.-T. Lin, Q. Lan, N. Rothman, S. Berndt P2 Missense polymorphic genetic combinations underlying down syndrome susceptibility E. S. Chen P4 The evaluation of alteration of ELAM-1 expression in the endometriosis patients H. Bahrami, A. Khoshzaban, S. Heidari Keshal P5 Obesity and the incidence of apolipoprotein E polymorphisms in an assorted population from Saudi Arabia population K. K. R. Alharbi P6 Genome-associated personalized antithrombotical therapy for patients with high risk of thrombosis and bleeding M. Zhalbinova, A. Akilzhanova, S. Rakhimova, M. Bekbosynova, S. Myrzakhmetova P7 Frequency of Xmn1 polymorphism among sickle cell carrier cases in UAE population M. Matar P8 Differentiating inflammatory bowel diseases by using genomic data: dimension of the problem and network organization N. Mili, R. Molinari, Y. Ma, S. Guerrier P9 Vulnerability of genetic variants to the risk of autism among Saudi children N. Elhawary, M. Tayeb, N. Bogari, N. Qotb P10 Chromatin profiles from ex vivo purified dopaminergic neurons establish a promising model to support studies of neurological function and dysfunction S. A. McClymont, P. W. Hook, L. A. Goff, A. McCallion P11 Utilization of a sensitized chemical mutagenesis screen to identify genetic modifiers of retinal dysplasia in homozygous Nr2e3rd7 mice Y. Kong, J. R. Charette, W. L. Hicks, J. K. Naggert, L. Zhao, P. M. Nishina P12 Ion torrent next generation sequencing of recessive polycystic kidney disease in Saudi patients B. M. Edrees, M. Athar, F. A. Al-Allaf, M. M. Taher, W. Khan, A. Bouazzaoui, N. A. Harbi, R. Safar, H. Al-Edressi, A. Anazi, N. Altayeb, M. A. Ahmed, K. Alansary, Z. Abduljaleel P13 Digital expression profiling of Purkinje neurons and dendrites in different subcellular compartments A. Kratz, P. Beguin, S. Poulain, M. Kaneko, C. Takahiko, A. Matsunaga, S. Kato, A. M. Suzuki, N. Bertin, T. Lassmann, R. Vigot, P. Carninci, C. Plessy, T. Launey P14 The evolution of imperfection and imperfection of evolution: the functional and functionless fractions of the human genome D. Graur P16 Species-independent identification of known and novel recurrent genomic entities in multiple cancer patients J. Friis-Nielsen, J. M. Izarzugaza, S. Brunak P18 Discovery of active gene modules which are densely conserved across multiple cancer types reveal their prognostic power and mutually exclusive mutation patterns B. S. Soibam P19 Whole exome sequencing of dysplastic leukoplakia tissue indicates sequential accumulation of somatic mutations from oral precancer to cancer D. Das, N. Biswas, S. Das, S. Sarkar, A. Maitra, C. Panda, P. Majumder P21 Epigenetic mechanisms of carcinogensis by hereditary breast cancer genes J. J. Gruber, N. Jaeger, M. Snyder P22 RNA direct: a novel RNA enrichment strategy applied to transcripts associated with solid tumors K. Patel, S. Bowman, T. Davis, D. Kraushaar, A. Emerman, S. Russello, N. Henig, C. Hendrickson P23 RNA sequencing identifies gene mutations for neuroblastoma K. Zhang P24 Participation of SFRP1 in the modulation of TMPRSS2-ERG fusion gene in prostate cancer cell lines M. Rodriguez-Dorantes, C. D. Cruz-Hernandez, C. D. P. Garcia-Tobilla, S. Solorzano-Rosales P25 Targeted Methylation Sequencing of Prostate Cancer N. Jäger, J. Chen, R. Haile, M. Hitchins, J. D. Brooks, M. Snyder P26 Mutant TPMT alleles in children with acute lymphoblastic leukemia from México City and Yucatán, Mexico S. Jiménez-Morales, M. Ramírez, J. Nuñez, V. Bekker, Y. Leal, E. Jiménez, A. Medina, A. Hidalgo, J. Mejía P28 Genetic modifiers of Alström syndrome J. Naggert, G. B. Collin, K. DeMauro, R. Hanusek, P. M. Nishina P31 Association of genomic variants with the occurrence of angiotensin-converting-enzyme inhibitor (ACEI)-induced coughing among Filipinos E. M. Cutiongco De La Paz, R. Sy, J. Nevado, P. Reganit, L. Santos, J. D. Magno, F. E. Punzalan , D. Ona , E. Llanes, R. L. Santos-Cortes , R. Tiongco, J. Aherrera, L. Abrahan, P. Pagauitan-Alan; Philippine Cardiogenomics Study Group P32 The use of “humanized” mouse models to validate disease association of a de novo GARS variant and to test a novel gene therapy strategy for Charcot-Marie-Tooth disease type 2D K. H. Morelli, J. S. Domire, N. Pyne, S. Harper, R. Burgess P34 Molecular regulation of chondrogenic human induced pluripotent stem cells M. A. Gari, A. Dallol, H. Alsehli, A. Gari, M. Gari, A. Abuzenadah P35 Molecular profiling of hematologic malignancies: implementation of a variant assessment algorithm for next generation sequencing data analysis and clinical reporting M. Thomas, M. Sukhai, S. Garg, M. Misyura, T. Zhang, A. Schuh, T. Stockley, S. Kamel-Reid P36 Accessing genomic evidence for clinical variants at NCBI S. Sherry, C. Xiao, D. Slotta, K. Rodarmer, M. Feolo, M. Kimelman, G. Godynskiy, C. O’Sullivan, E. Yaschenko P37 NGS-SWIFT: a cloud-based variant analysis framework using control-accessed sequencing data from DBGAP/SRA C. Xiao, E. Yaschenko, S. Sherry P38 Computational assessment of drug induced hepatotoxicity through gene expression profiling C. Rangel-Escareño, H. Rueda-Zarate P40 Flowr: robust and efficient pipelines using a simple language-agnostic approach;ultraseq; fast modular pipeline for somatic variation calling using flowr S. Seth, S. Amin, X. Song, X. Mao, H. Sun, R. G. Verhaak, A. Futreal, J. Zhang P41 Applying “Big data” technologies to the rapid analysis of heterogenous large cohort data S. J. Whiite, T. Chiang, A. English, J. Farek, Z. Kahn, W. Salerno, N. Veeraraghavan, E. Boerwinkle, R. Gibbs P42 FANTOM5 web resource for the large-scale genome-wide transcription start site activity profiles of wide-range of mammalian cells T. Kasukawa, M. Lizio, J. Harshbarger, S. Hisashi, J. Severin, A. Imad, S. Sahin, T. C. Freeman, K. Baillie, A. Sandelin, P. Carninci, A. R. R. Forrest, H. Kawaji, The FANTOM Consortium P43 Rapid and scalable typing of structural variants for disease cohorts W. Salerno, A. English, S. N. Shekar, A. Mangubat, J. Bruestle, E. Boerwinkle, R. A. Gibbs P44 Polymorphism of glutathione S-transferases and sulphotransferases genes in an Arab population A. H. Salem, M. Ali, A. Ibrahim, M. Ibrahim P46 Genetic divergence of CYP3A5*3 pharmacogenomic marker for native and admixed Mexican populations J. C. Fernandez-Lopez, V. Bonifaz-Peña, C. Rangel-Escareño, A. Hidalgo-Miranda, A. V. Contreras P47 Whole exome sequence meta-analysis of 13 white blood cell, red blood cell, and platelet traits L. Polfus, CHARGE and NHLBI Exome Sequence Project Working Groups P48 Association of adipoq gene with type 2 diabetes and related phenotypes in african american men and women: The jackson heart study S. Davis, R. Xu, S. Gebeab, P Riestra, A Gaye, R. Khan, J. Wilson, A. Bidulescu P49 Common variants in casr gene are associated with serum calcium levels in koreans S. H. Jung, N. Vinayagamoorthy, S. H. Yim, Y. J. Chung P50 Inference of multiple-wave population admixture by modeling decay of linkage disequilibrium with multiple exponential functions Y. Zhou, S. Xu P51 A Bayesian framework for generalized linear mixed models in genome-wide association studies X. Wang, V. Philip, G. Carter P52 Targeted sequencing approach for the identification of the genetic causes of hereditary hearing impairment A. A. Abuzenadah, M. Gari, R. Turki, A. Dallol P53 Identification of enhancer sequences by ATAC-seq open chromatin profiling A. Uyar, A. Kaygun, S. Zaman, E. Marquez, J. George, D. Ucar P54 Direct enrichment for the rapid preparation of targeted NGS libraries C. L. Hendrickson, A. Emerman, D. Kraushaar, S. Bowman, N. Henig, T. Davis, S. Russello, K. Patel P56 Performance of the Agilent D5000 and High Sensitivity D5000 ScreenTape assays for the Agilent 4200 Tapestation System R. Nitsche, L. Prieto-Lafuente P57 ClinVar: a multi-source archive for variant interpretation M. Landrum, J. Lee, W. Rubinstein, D. Maglott P59 Association of functional variants and protein physical interactions of human MUTY homolog linked with familial adenomatous polyposis and colorectal cancer syndrome Z. Abduljaleel, W. Khan, F. A. Al-Allaf, M. Athar , M. M. Taher, N. Shahzad P60 Modification of the microbiom constitution in the gut using chicken IgY antibodies resulted in a reduction of acute graft-versus-host disease after experimental bone marrow transplantation A. Bouazzaoui, E. Huber, A. Dan, F. A. Al-Allaf, W. Herr, G. Sprotte, J. Köstler, A. Hiergeist, A. Gessner, R. Andreesen, E. Holler P61 Compound heterozygous mutation in the LDLR gene in Saudi patients suffering severe hypercholesterolemia F. Al-Allaf, A. Alashwal, Z. Abduljaleel, M. Taher, A. Bouazzaoui, H. Abalkhail, A. Al-Allaf, R. Bamardadh, M. Atha

The James Webb Space Telescope Mission

Twenty-six years ago a small committee report, building on earlier studies, expounded a compelling and poetic vision for the future of astronomy, calling for an infrared-optimized space telescope with an aperture of at least $4m$. With the support of their governments in the US, Europe, and Canada, 20,000 people realized that vision as the $6.5m$ James Webb Space Telescope. A generation of astronomers will celebrate their accomplishments for the life of the mission, potentially as long as 20 years, and beyond. This report and the scientific discoveries that follow are extended thank-you notes to the 20,000 team members. The telescope is working perfectly, with much better image quality than expected. In this and accompanying papers, we give a brief history, describe the observatory, outline its objectives and current observing program, and discuss the inventions and people who made it possible. We cite detailed reports on the design and the measured performance on orbit.Comment: Accepted by PASP for the special issue on The James Webb Space Telescope Overview, 29 pages, 4 figure

Effects of Once-Weekly Exenatide on Cardiovascular Outcomes in Type 2 Diabetes.

Abstract BACKGROUND: The cardiovascular effects of adding once-weekly treatment with exenatide to usual care in patients with type 2 diabetes are unknown. METHODS: We randomly assigned patients with type 2 diabetes, with or without previous cardiovascular disease, to receive subcutaneous injections of extended-release exenatide at a dose of 2 mg or matching placebo once weekly. The primary composite outcome was the first occurrence of death from cardiovascular causes, nonfatal myocardial infarction, or nonfatal stroke. The coprimary hypotheses were that exenatide, administered once weekly, would be noninferior to placebo with respect to safety and superior to placebo with respect to efficacy. RESULTS: In all, 14,752 patients (of whom 10,782 [73.1%] had previous cardiovascular disease) were followed for a median of 3.2 years (interquartile range, 2.2 to 4.4). A primary composite outcome event occurred in 839 of 7356 patients (11.4%; 3.7 events per 100 person-years) in the exenatide group and in 905 of 7396 patients (12.2%; 4.0 events per 100 person-years) in the placebo group (hazard ratio, 0.91; 95% confidence interval [CI], 0.83 to 1.00), with the intention-to-treat analysis indicating that exenatide, administered once weekly, was noninferior to placebo with respect to safety (P<0.001 for noninferiority) but was not superior to placebo with respect to efficacy (P=0.06 for superiority). The rates of death from cardiovascular causes, fatal or nonfatal myocardial infarction, fatal or nonfatal stroke, hospitalization for heart failure, and hospitalization for acute coronary syndrome, and the incidence of acute pancreatitis, pancreatic cancer, medullary thyroid carcinoma, and serious adverse events did not differ significantly between the two groups. CONCLUSIONS: Among patients with type 2 diabetes with or without previous cardiovascular disease, the incidence of major adverse cardiovascular events did not differ significantly between patients who received exenatide and those who received placebo. (Funded by Amylin Pharmaceuticals; EXSCEL ClinicalTrials.gov number, NCT01144338 .)

Dietas Artificiais para a Criação de Ceratitis capitata (Wiedemann 1824) (Diptera: Tephritidae)

Ceratitis capitata (Wiedemann 1824)(Diptera: Tephritidae) is a polyphagous insect responsible for severe damage to fruit production worldwide. Control strategies require laboratory studies in which large numbers of individuals of this pest are required, thus requiring the implementation of mass creations. The success of these creations depends on the use of artificial diets, which represent one of the main costs of this system. In this sense, this study aimed to evaluate the efficiency of different artificial diets based on regional foods in the development of C. capitata in the laboratory. Eggs of C. capitata were inoculated in artificial diets based on sweet potato, yam, carrot, pumpkin and cassava, all in the raw and cooked version, totaling ten treatments, with raw carrot being the control treatment. Variables related to the biometric and biological characteristics of C. capitata, such as larval and pupal viability, fecundity, fertility and sex ratio, in addition to pre-oviposition, oviposition and adult life times, were evaluated. Promising results were obtained with the use of artificial diets based on regional foods, such as sweet potato and pumpkin, in which higher pupae weight and size, good fecundity and fertility, insects with longer oviposition time and longer longevity were observed, whose results were similar or superior to those obtained with the raw carrot-based diet. In contrast, the raw cassava-based diet did not allow larvae to hatch. Unsatisfactory results were also obtained with diets based on yam, both raw and cooked, which makes its recommendation for use in artificial diets for this insect unfeasible. Artificial diets derived from pumpkin and sweet potato, raw or cooked, are efficient as carrot substitutes in artificial diets of C. capitata.Ceratitis capitata (Wiedemann 1824) (Diptera: Tephritidae) é um inseto polífago responsável por severos danos a fruticultura mundial. Estratégias de controle requerem estudos laboratoriais em que são demandadas grandes quantidades de indivíduos dessa praga, necessitando portanto, da implantação de criações massais. O sucesso dessas criações é dependente do uso de dietas artificiais, que representam um dos principais custos desse sistema. Nesse sentido, objetivou-se avaliar o crescimento e desenvolvimento de C. capitata sob diferentes dietas artificiais. A inoculação dos ovos de C. capitata foi realizado em dietas artificiais a base de batata-doce, cará, cenoura, jerimum e macaxeira, todos na versão crua e cozida, totalizando dez tratamentos, sendo a cenoura crua o tratamento controle. Observou-se as seguintes variáveis biométricas e biológicas de C. capitata: viabilidade larval e pupal, fecundidade, fertilidade e razão sexual, além dos tempos de pré-oviposição, oviposição e de vida dos adultos. Obteve-se resultados promissores com batata-doce e jerimum, proporcionando insetos maiores e mais pesados, boa fecundidade e fertilidade, maior tempo de oviposição e maior longevidade, cujos resultados foram semelhantes ou superiores aos obtidos com a dieta a base de cenoura crua. Em contraste, a dieta a base de macaxeira crua não permitiu a eclosão das larvas. Resultados insatisfatórios também foram obtidos com as dietas a base de cará, tanto cru como cozido, o que inviabiliza sua recomendação para utilização em dietas artificiais para esse inseto. As dietas artificiais derivadas de jerimum e batata-doce, crus ou cozidos, se mostram eficientes como substitutos da cenoura em dietas artificiais de C. capitata

CRESCIMENTO E PRODUÇÃO DA ALFACE SOB DIFERENTES REPOSIÇÕES HÍDRICAS E USO DE POLÍMERO HIDRORETENTOR

A alface &nbsp;é uma hortaliça bastante exigente em água, o que pode limitar sua produção em regiões com carência hídrica. Assim,&nbsp; o uso de tecnologias que permitam a maximização do uso da água nessas áreas se faz necessária. Nesse sentido, objetivou-se avaliar o crescimento e produção da alface utilizando diferentes reposições hídricas e polímero hidroretentor. O experimento foi conduzido em casa de vegetação em delineamento de blocos casualizados, utilizando-se esquema fatorial 4 x 2 + 2, referentes as seguintes lâminas de irrigação (100%; 80%; 60%; 40% da capacidade de campo), e dois volumes de polímero hidroretentor (50 e 25 mL por planta), além de duas testemunhas, &nbsp;com quatro repetições. Foram determinadas a altura da planta; diâmetro da cabeça; número de folhas por planta; massa fresca e massa da matéria seca da parte aérea (caule + folhas). As doses de polímero hidroretentor não influenciaram &nbsp;em nenhuma das variáveis avaliadas. Para as reposições hídricas utilizadas, a lâmina de água de 100% da capacidade de campo resultou nas maiores médias para todas as variáveis avaliadas